Product datasheet
Anti-CLEC2D antibody ab13664
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Overview
Product name
Anti-CLEC2D antibody
Description
Mouse monoclonal to CLEC2D
Tested applications
WB
Species reactivity
Reacts with: Mouse
Immunogen
Synthetic peptide:
CVTKASLPMLSPTGSPQ
, corresponding to amino acids 2-18 of CLEC2D (OCIL).
Run BLAST with
Positive control
Run BLAST with
Mouse spleen cell lysates.
General notes
Recently, Zhou et al have identified a protein that can inhibit multinucleate Osteoclast formation
in murine osteoblast and spleen cell co-cultures. This protein called, Osteoclast Inhibitory Lectin
(OCIL) can also inhibit osteoclast formation in spleen cell cultures treated with RANKL and MCSF. Furthermore, mOCIL acted directly on macrophage/monocyte cells as evidenced by its
inhibitory action on adherent spleen cell cultures, which were depleted of stromal and
lymphocytic cells. mOCIL completely inhibited osteoclast formation during the proliferative phase
of osteoclast formation and resulted in 70% inhibition during the differentiation phase.
Osteoblast OCIL mRNA expression was enhanced by parathyroid hormone, calcitriol,
interleukin-1 and IL-11, and retinoic acid. OCIL is expressed in osteoblasts and chondrocytes as
well as in a variety of extraskeletal tissues. Since OCIL expression was found to be very similar
to distribution for RANKL, it has been suggested that RANKl and OCIL may interact in skeleton
and in extracellular tissues.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks) (add antibacterial agent). Aliquot and store at -20°C or 80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Purity
IgG fraction
Primary antibody notes
Recently, Zhou et al have identified a protein that can inhibit multinucleate Osteoclast formation
in murine osteoblast and spleen cell co-cultures. This protein called, Osteoclast Inhibitory Lectin
(OCIL) can also inhibit osteoclast formation in spleen cell cultures treated with RANKL and M1
CSF. Furthermore, mOCIL acted directly on macrophage/monocyte cells as evidenced by its
inhibitory action on adherent spleen cell cultures, which were depleted of stromal and
lymphocytic cells. mOCIL completely inhibited osteoclast formation during the proliferative phase
of osteoclast formation and resulted in 70% inhibition during the differentiation phase.
Osteoblast OCIL mRNA expression was enhanced by parathyroid hormone, calcitriol,
interleukin-1 and IL-11, and retinoic acid. OCIL is expressed in osteoblasts and chondrocytes as
well as in a variety of extraskeletal tissues. Since OCIL expression was found to be very similar
to distribution for RANKL, it has been suggested that RANKl and OCIL may interact in skeleton
and in extracellular tissues.
Clonality
Monoclonal
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab13664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 25 kDa.
Target
Function
Receptor for KLRB1 that protects target cells against natural killer cell-mediated lysis. Inhibits
osteoclast formation. Inhibits bone resorption. Modulates the release of interferon-gamma. Binds
high molecular weight sulfated glycosaminoglycans.
Tissue specificity
Detected in peripheral blood leukocytes, osteoblasts, lymph node, thymus and spleen. Isoform 1,
isoform 2 and isoform 4 are expressed in T and B lymphocytes, and at lower levels in NK cells.
They are also expressed in B-cell lines and LPS-matured monocyte-derived dendritic cells.
Sequence similarities
Contains 1 C-type lectin domain.
Cellular localization
Endoplasmic reticulum and Cell membrane. Cell surface.
Anti-CLEC2D antibody images
Western blot analysis of CLEC2D (OCIL) in
mouse spleen cell lysates. Thirty microgram
of cell lysates per lane of mini gel was
analyzed in Western blotting using ab1664.
Western blot - CLEC2D antibody (ab13664)
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