Product datasheet
Multicolor Human B cell marker panel (CD5-PE, CD19APC, CD20-FITC and CD45-PE/Cy7®) ab106071
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Overview
Product name
Multicolor Human B cell marker panel (CD5-PE, CD19-APC, CD20-FITC and CD45-PE/Cy7®)
Product overview
ab106071 is a human B cell marker panel containing CD5 mouse monoclonal (PE-conjugated),
CD19 mouse monoclonal (APC-conjugated), CD20 mouse monoclonal (FITC-conjugated) and
CD45 mouse monoclonal (PE/Cy7-conjugated) antibodies, tested for use as a cocktail in flow
cytometry.
The human B cell marker panel contains a selection of the most popular B cell associated CD
markers, conjugated to a wide spectrum of fluorochromes for optimal detection using flow
cytometry. Matched isotype controls are available (ab91363, ab91358, ab81197, ab103534).
Components
Anti-CD19 antibody [LT19] (Allophycocyanin) (ab18224)
Flow Cyt, IP
Mouse monoclonal (50tests )
0.5mg/ml
Reacts with Hu
Anti-CD45 antibody [F10-89-4] (PE/Cy7 ®) (ab46729)
Flow Cyt, IHC-Fr, IP, WB
Mouse monoclonal (50tests )
Reacts with Hu
Anti-CD5 antibody [CRIS1] (Phycoerythrin) (ab1157)
Flow Cyt
Mouse monoclonal (50tests )
Reacts with Hu
Anti-CD20 antibody [B9E9] (FITC) (ab1169)
Flow Cyt
Mouse Monoclonal (50tests )
Reacts with Hu
Anti-CD20 antibody [B9E9] (FITC) (ab1169)
Flow Cyt
Mouse Monoclonal (50tests )
Reacts with Hu
Applications
Our Abpromise guarantee covers the use of ab106071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
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Application
Abreviews
Flow Cyt
Notes
Use at an assay dependent dilution. Typical usage: 2.5-5µl of each antibody for
106 cells. Optimal volumes should be determined by the end user.
Multicolor Human B cell marker panel (CD5-PE, CD19-APC, CD20-FITC and CD45-PE/Cy7®) images
ab106071 staining of B cells in whole human
blood by flow cytometry.
Peripheral blood was collected into Li
Heparin LH/9 ml monovettes. Red blood cells
were lysed with lysis buffer (for 5 ml: 41.45 g
NH4Cl, 5 g NaHCO3, 1 ml 0.5M EDTA in
H2O) for 20 min at room temperature. After
spinning down, the obtained cell pellet was
washed twice with PBS. The cells were
stained with the antibodies (2.5 – 5 µl of each
Flow Cytometry - Multicolor Human B cell marker
antibody/1x106 cells in 100 µl) for 20 min at
panel (CD5-PE, CD19-APC, CD20-FITC and
room temperature in the dark. Samples were
CD45-PE/Cy7®) (ab106071)
washed twice with cell sorting buffer (3 %
FCS in PBS) and resuspended. Flow
cytometry was performed on a FACSCalibur
(Becton Dickinson) at 488 nm (FL-1, 2, 3) and
633 nm (FL-4), and analysed using Flojo
software.
Matched isotype control antibodies were
used under the same conditions (ab81197,
ab91363, ab91358, ab103534).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
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Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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