Product datasheet
Anti-EPX antibody [EPO104] ab199005
Overview
Product name
Anti-EPX antibody [EPO104]
Description
Mouse monoclonal [EPO104] to EPX
Tested applications
IHC-Fr, Flow Cyt, ICC/IF
Species reactivity
Reacts with: Human
Immunogen
Tissue, cells or virus corresponding to Human EPX. Human eosinophils from a patient with
hypereosinophilic syndrome
Positive control
MCF-7 cells. Tonsil
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Purity
Protein G purified
Purification notes
purified from Bioreactor Concentrates
Clonality
Monoclonal
Clone number
EPO104
Isotype
IgG1
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab199005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-Fr
Abreviews
Notes
Use a concentration of 0.5 - 1 µg/ml.
30 mins room temperature
1
Application
Abreviews
Notes
Use 0.5-1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use
Flow Cyt
as an isotype control with this antibody.
ICC/IF
Use a concentration of 0.5 - 1 µg/ml.
Target
Function
Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows
significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial
fragmentation and lysis.
Involvement in disease
Defects in EPX are the cause of eosinophil peroxidase deficiency (EPD) [MIM:261500]. EPD is
an autosomal recessive defect where anomalous eosinophils are characterized by nuclear
hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining.
Sequence similarities
Belongs to the peroxidase family. XPO subfamily.
Cellular localization
Cytoplasmic granule. Cytoplasmic granules of eosinophils.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
2