Product datasheet
Anti-FBXL5 antibody ab140175
2 Abreviews 4 Images
Overview
Product name
Anti-FBXL5 antibody
Description
Rabbit polyclonal to FBXL5
Tested applications
ICC/IF, WB, IHC-P
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
Immunogen
Recombinant fragment, corresponding to amino acids 510-691 of Human FBXL5 (BC030656)
Positive control
Human fetal testis tissue.
Properties
Form
Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this
product in liquid form it has already been reconstituted as described and no further reconstitution
is necessary.
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab140175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
Use at an assay dependent concentration.
WB
1/1000 - 1/2000. Predicted molecular weight: 78 kDa.
IHC-P
1/100 - 1/500.
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Target
Function
Component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in
iron homeostasis by promoting the ubiquitination and subsequent degradation of IREB2/IRP2.
Upon high iron and oxygen level, it specifically recognizes and binds IREB2/IRP2, promoting its
ubiquitination and degradation by the proteasome. Promotes ubiquitination and subsequent
degradation of DCTN1/p150-glued.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 1 F-box domain.
Contains 7 LRR (leucine-rich) repeats.
Domain
The hemerythrin-like region acts as an oxygen and iron sensor by binding oxygen through a
diiron metal-center. In absence of oxygen and iron, the protein is ubiquitinated and degraded.
Post-translational
modifications
Ubiquitinated upon iron and oxygen depletion, leading to its degradation by the proteasome.
Ubiquitination is regulated by the hemerythrin-like region that acts as an oxygen and iron sensor.
Cellular localization
Cytoplasm > perinuclear region.
Anti-FBXL5 antibody images
ab140175 staining FBXL5 in Mouse 14.5dpc
tissue sections by Immunohistochemistry
(IHC-P - paraformaldehyde-fixed, paraffinembedded sections). Tissue was fixed with
formaldehyde and blocked with 1% BSA
for 1 hour at room temperature; antigen
retrieval was by heat mediation in Tris pH 9.
Samples were incubated with primary
antibody (1/100 in 1% BSA + 1%FBS in TBS)
Immunohistochemistry (Formalin/PFA-fixed
for 16 hours. An undiluted HRP-conjugated
paraffin-embedded sections) - Anti-FBXL5
Goat anti-rabbit IgG polyclonal was used as
antibody (ab140175)
the secondary antibody.
This image is courtesy of an anonymous Abreview
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ab140175 staining FBXL5 in MCF7 cells by
ICC/IF
(Immunocytochemistry/immunofluorescence).
Cells were fixed with formaldehyde,
Immunocytochemistry/ Immunofluorescence Anti-FBXL5 antibody (ab140175)
This image is courtesy of an anonymous Abreview
permeabilized with 0.5% Tween-20 in PBS
and blocked with 3% BSA for 1 hour at room
temperature. Samples were incubated with
primary antibody (1/50 in PBS + 0.02%
Tween-20 + 1% BSA) for 1 hour at 27°C. An
Alexa Fluor®-conjugated Donkey anti-rabbit
IgG polyclonal (1/500) was used as the
secondary antibody. Left - nuclei, middle FBXL5, right - merge.
Anti-FBXL5 antibody (ab140175) at 1/1000
dilution + Human fetal testis lysate
Predicted band size : 78 kDa
Western blot - Anti-FBXL5 antibody (ab140175)
Immunohistochemical analysis of formalinfixed, parafin-embedded Human fetal testis
tissue labelling FBXL5 with ab140175 at
1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-FBXL5
antibody (ab140175)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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