Sakaguchi Lab
Updated 03/2001
Tract-Tracing References
Some useful references for various tract-tracing procedures. This is not an all inclusive list.
The purpose of this reference list is to provide a starting point of some useful references. No
particular order to this list.
1: Ino T, Kaneko T, Mizuno N.
Projections from the hippocampal and parahippocampal regions to the entorhinal cortex. An
anterograde and retrograde tract-tracing study in the cat.
Neurosci Res. 2001 Jan;39(1):51-69.
2: Perez SE, Yanez J, Marin O, Anadon R, Gonzalez A, Rodriguez-Moldes I.
Distribution of choline acetyltransferase (ChAT) immunoreactivity in the brain of the adult trout
and tract-tracing observations on the connections of the nuclei of the isthmus.
J Comp Neurol. 2000 Dec 18;428(3):450-74.
3: Lanciego JL, Wouterlood FG, Erro E, Arribas J, Gonzalo N, Urra X, Cervantes S, GimenezAmaya JM.
Complex brain circuits studied via simultaneous and permanent detection of three transported
neuroanatomical tracers in the same histological section.
J Neurosci Methods. 2000 Nov 15;103(1):127-35.
4: Sparks DL, Lue LF, Martin TA, Rogers J.
Neural tract tracing using Di-I: a review and a new method to make fast Di-I faster in human brain.
J Neurosci Methods. 2000 Nov 15;103(1):3-10. Review.
5: Lanciego JL, Wouterlood FG.
Neuroanatomical tract-tracing methods beyond 2000: what's new and next
J Neurosci Methods. 2000 Nov 15;103(1):1-2. No abstract available.
6: Campeau S, Watson SJ.
Connections of some auditory-responsive posterior thalamic nuclei putatively involved in
activation of the hypothalamo-pituitary-adrenocortical axis in response to audiogenic stress in rats:
an anterograde and retrograde tract tracing study combined with Fos expression.
J Comp Neurol. 2000 Jul 31;423(3):474-91.
7: Kobbert C, Apps R, Bechmann I, Lanciego JL, Mey J, Thanos S.
Current concepts in neuroanatomical tracing.
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Sakaguchi Lab
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Prog Neurobiol. 2000 Nov;62(4):327-51. Review.
8: Chang SL, LoTurco JJ, Nisenbaum LK.
In vitro biocytin injection into perinatal mouse brain: a method for tract tracing in developing
tissue.
J Neurosci Methods. 2000 Apr 1;97(1):1-6.
9: Sequeira H, Poulain P, Ba-M'Hamed S, Viltart O.
Immunocytochemical detection of fos protein combined with anterograde tract-tracing using
biotinylated dextran.
Brain Res Brain Res Protoc. 2000 Feb;5(1):49-56.
10: Wang CC, Willis WD, Westlund KN.
Ascending projections from the area around the spinal cord central canal: A Phaseolus vulgaris
leucoagglutinin study in rats.
J Comp Neurol. 1999 Dec 20;415(3):341-67.
11: Kamada K, Pekar JJ, Kanwal JS.
Anatomical and functional imaging of the auditory cortex in awake mustached bats using magnetic
resonance technology.
Brain Res Brain Res Protoc. 1999 Dec;4(3):351-9.
12: Coolen LM, Jansen HT, Goodman RL, Wood RI, Lehman MN.
A new method for simultaneous demonstration of anterograde and retrograde connections in the
brain: co-injections of biotinylated dextran amine and the beta subunit of cholera toxin.
J Neurosci Methods. 1999 Sep 15;91(1-2):1-8.
13: Erro E, Lanciego JL, Gimenez-Amaya JM.
Relationships between thalamostriatal neurons and pedunculopontine projections to the thalamus: a
neuroanatomical tract-tracing study in the rat.
Exp Brain Res. 1999 Jul;127(2):162-70.
14: Jones EG.
Making brain connections: neuroanatomy and the work of TPS Powell, 1923-1996.
Annu Rev Neurosci. 1999;22:49-103. Review.
15: Romero MI, Romero MA, Smith GM.
Visualization of axonally transported horseradish peroxidase using enhanced immunocytochemical
detection: a direct comparison with the tetramethylbenzidine method.
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Sakaguchi Lab
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J Histochem Cytochem. 1999 Feb;47(2):265-72.
16: Bianchi R, Corsetti G, Rodella L, Tredici G, Gioia M.
Supraspinal connections and termination patterns of the parabrachial complex determined by the
biocytin anterograde tract-tracing technique in the rat.
J Anat. 1998 Oct;193 ( Pt 3):417-30.
17: Lanciego JL, Wouterlood FG, Erro E, Gimenez-Amaya JM.
Multiple axonal tracing: simultaneous detection of three tracers in the same section.
Histochem Cell Biol. 1998 Nov;110(5):509-15.
18: Norgren RB, Lehman MN.
Herpes simplex virus as a transneuronal tracer.
Neurosci Biobehav Rev. 1998 Oct;22(6):695-708. Review.
19: Pautler RG, Silva AC, Koretsky AP.
In vivo neuronal tract tracing using manganese-enhanced magnetic resonance imaging.
Magn Reson Med. 1998 Nov;40(5):740-8.
20: Zupanc GK.
An in vitro technique for tracing neuronal connections in the teleost brain.
Brain Res Brain Res Protoc. 1998 Sep;3(1):37-51.
21: Coolen LM, Wood RI.
Bidirectional connections of the medial amygdaloid nucleus in the Syrian hamster brain:
simultaneous anterograde and retrograde tract tracing.
J Comp Neurol. 1998 Sep 21;399(2):189-209.
22: Yanez J, Anadon R.
Neural connections of the pineal organ in the primitive bony fish Acipenser baeri: a carbocyanine
dye tract-tracing study.
J Comp Neurol. 1998 Aug 24;398(2):151-61.
23: Erisir A, Aoki C.
A method of combining biocytin tract-tracing with avidin-biotin-peroxidase complex
immunocytochemistry for pre-embedding electron microscopic labeling in neonatal tissue.
J Neurosci Methods. 1998 Jun 1;81(1-2):189-97.
24: Lanciego JL, Luquin MR, Guillen J, Gimenez-Amaya JM.
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Sakaguchi Lab
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Multiple neuroanatomical tracing in primates.
Brain Res Brain Res Protoc. 1998 Jun;2(4):323-32.
25: Chamberlin NL, Du B, de Lacalle S, Saper CB.
Recombinant adeno-associated virus vector: use for transgene expression and anterograde tract
tracing in the CNS.
Brain Res. 1998 May 18;793(1-2):169-75.
26: Franzoni MF, Jansen K, Feuilloley M, Vaudry H, Fasolo A.
Analysis of the projections to amphibian pars intermedia by combined tract tracing,
immunohistochemistry and confocal microscopy.
Ann N Y Acad Sci. 1998 May 15;839:351-2. No abstract available.
27: Hallbook F, Fritzsch B.
Distribution of BDNF and trkB mRNA in the otic region of 3.5 and 4.5 day chick embryos as
revealed with a combination of in situ hybridization and tract tracing.
Int J Dev Biol. 1997 Oct;41(5):725-32.
28: van der Want JJ, Klooster J, Cardozo BN, de Weerd H, Liem RS.
Tract-tracing in the nervous system of vertebrates using horseradish peroxidase and its conjugates:
tracers, chromogens and stabilization for light and electron microscopy.
Brain Res Brain Res Protoc. 1997 Aug;1(3):269-79.
29: Senatorov VV, Trudeau VL, Hu B.
Immunofluorescence in situ hybridization (IFISH) in neurones retrogradely labelled with
rhodamine latex microspheres.
Brain Res Brain Res Protoc. 1997 Feb;1(1):49-56.
30: Toth IE, Palkovits M.
Viral labelling of synaptically connected neurons.
Neurobiology (Bp). 1997;5(1):17-41. Review.
31: Van der Beek EM, Horvath TL, Wiegant VM, Van den Hurk R, Buijs RM.
Evidence for a direct neuronal pathway from the suprachiasmatic nucleus to the gonadotropinreleasing hormone system: combined tracing and light and electron microscopic
immunocytochemical studies.
J Comp Neurol. 1997 Aug 11;384(4):569-79.
32: Fritzsch B, Hallbook F.
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Sakaguchi Lab
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A simple and reliable technique to combine oligonucleotide probe in situ hybridization with
neuronal tract tracing in vertebrate embryos.
Biotech Histochem. 1996 Nov;71(6):289-94.
33: Dong K, Qu T, Ahmed FA, Zhang L, Yamada K, Guison NG, Miller M, Yamadori T.
Fluoro-Green and Fluoro-Red: two new fluorescent retrograde tracers with a number of unique
properties.
Brain Res. 1996 Oct 14;736(1-2):61-7.
34: Mcdonald AJ, Mascagni F, Guo L.
Projections of the medial and lateral prefrontal cortices to the amygdala: a Phaseolus vulgaris
leucoagglutinin study in the rat.
Neuroscience. 1996 Mar;71(1):55-75.
35: Strack AM.
Pseudorabies virus as a transneuronal tract tracing tool: specificity and applications to the
sympathetic nervous system.
Gene Ther. 1994;1 Suppl 1:S11-4.
36: Rajakumar N, Elisevich K, Flumerfelt BA.
Biotinylated dextran: a versatile anterograde and retrograde neuronal tracer.
Brain Res. 1993 Apr 2;607(1-2):47-53.
37: McDonald AJ.
Neuroanatomical labeling with biocytin: a review.
Neuroreport. 1992 Oct;3(10):821-7. Review.
38: Tourtellotte WG, Van Hoesen GW.
Computer-aided two-dimensional high-resolution axon tracing: an application using the
anterograde tracer Phaseolus vulgaris leucoagglutinin (PHAL).
J Neurosci Methods. 1992 Feb;41(2):101-12.
39: McDonald AJ.
Projection neurons of the basolateral amygdala: a correlative Golgi and retrograde tract tracing
study.
Brain Res Bull. 1992 Feb;28(2):179-85.
40: Huang Q, Zhou D, DiFiglia M.
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Sakaguchi Lab
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Neurobiotin, a useful neuroanatomical tracer for in vivo anterograde, retrograde and transneuronal
tract-tracing and for in vitro labeling of neurons.
J Neurosci Methods. 1992 Jan;41(1):31-43.
41: Lapper SR, Bolam JP.
The anterograde and retrograde transport of neurobiotin in the central nervous system of the rat:
comparison with biocytin.
J Neurosci Methods. 1991 Sep;39(2):163-74.
42: Dederen PJ, Joosten EA.
A triple-labeling method: HRP anterograde tract tracing combined with double immunofluorescent
cell staining in developing neural tissue of the rat.
J Neurosci Methods. 1989 Oct;30(1):71-6.
43: King MA, Louis PM, Hunter BE, Walker DW.
Biocytin: a versatile anterograde neuroanatomical tract-tracing alternative.
Brain Res. 1989 Sep 18;497(2):361-7.
44: Schmued LC, Kyriakidis K, Fallon JH, Ribak CE.
Neurons containing retrogradely transported Fluoro-Gold exhibit a variety of lysosomal profiles: a
combined brightfield, fluorescence, and electron microscopic study.
J Neurocytol. 1989 Jun;18(3):333-43.
45: Ter Horst GJ, Mast JG, Vaartjes K.
A device for enhancing the visibility of the tip of a glass micropipette: application in
neuroanatomy.
Brain Res Bull. 1988 Dec;21(6):917-8.
46: Thompson AM, Thompson GC.
Neural connections identified with PHA-L anterograde and HRP retrograde tract-tracing
techniques.
J Neurosci Methods. 1988 Aug;25(1):13-7.
47: Redgrave P, Mitchell IJ, Dean P.
Descending projections from the superior colliculus in rat: a study using orthograde transport of
wheatgerm-agglutinin conjugated horseradish peroxidase.
Exp Brain Res. 1987;68(1):147-67.
48: Bazer GT, Ebbesson SO.
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Sakaguchi Lab
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A simplified cobalt-lysine method for tracing axon trajectories in the central nervous system of
vertebrates.
Neurosci Lett. 1984 Oct 26;51(3):315-8.
49: Cuello AC, Priestley JV, Sofroniew MV.
Immunocytochemistry and neurobiology.
Q J Exp Physiol. 1983 Oct;68(4):545-78. Review.
50: de Olmos JS.
An improved HRP method for the study of central nervous connections.
Exp Brain Res. 1977 Sep 28;29(3-4):541-51.
51: Elberger AJ, Honig MG.
Double-labeling of tissue containing the carbocyanine dye DiI for immunocytochemistry.
J Histochem Cytochem. 1990 May;38(5):735-9.
52: Hilbig H, Schierwagen A.
Interlayer neurones in the rat superior colliculus: a tracer study using Dil/Di-ASP.
Neuroreport. 1994 Jan 12;5(4):477-80.
53: Honig MG, Hume RI.
Carbocyanine dyes. Novel markers for labelling neurons.
Trends Neurosci. 1989 Sep;12(9):336-8. No abstract available.
54: Honig MG, Hume RI.
Dil and diO: versatile fluorescent dyes for neuronal labelling and pathway tracing.
Trends Neurosci. 1989 Sep;12(9):333-5, 340-1. Review.
55: Kim GJ, Shatz CJ, McConnell SK.
Morphology of pioneer and follower growth cones in the developing cerebral cortex.
J Neurobiol. 1991 Sep;22(6):629-42.
56: Thanos S.
Bidirectional fluorescent labelling techniques for the developing and regenerating visual system.
Acta Histochem Suppl. 1990;38:259-67.
57: Thanos S.
Specific transcellular carbocyanine-labelling of rat retinal microglia during injury-induced neuronal
degeneration.
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Sakaguchi Lab
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Neurosci Lett. 1991 Jun 10;127(1):108-12.
58: von Bartheld CS, Cunningham DE, Rubel EW.
Neuronal tracing with DiI: decalcification, cryosectioning, and photoconversion for light and
electron microscopic analysis.
J Histochem Cytochem. 1990 May;38(5):725-33.
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