ER Stress pathway
Key
Kinase
Secretion
Phosphatase
Transcription
Factor
Ligand
ATF6
(p90)
Golgi Apparatus
Endoplasmic
Reticulum
Apoptos
Apoptosis
poptosiis
Caspase 3
Unfolded
Protein
ATF6
(p90)
IRE1
ROS
PERK
IRE1
P
Caspase 12
Bcl-2
Proteasomal
Degradation
Derlin
Pro Caspase 12
P
P
P
TRAF2
ASK1
Cytochrome C
ATF6
(p50)
elF-2α
Translation
ATF4
P
XBP1 mRNA splicing
PP1
GADD34
XBP1s
JNK
Mitochondria
ATF6
(p50)
CHOP
ATF4
Nucleus
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XBP1s
ERSEs
E
R
UPR genes (GRP78, PDI, GRP94, etc)
EDEM
ER Stress pathway
Antibody
Clonality
Application
Host
Species reactivity
t
uc ts
od gh
Pr hli
g
hi
Related antibodies
Product code
Bcl2 antibody [E17]
M
Flow Cyt, ICC/IF, IHC-P, IP, WB
Rb
Hu, Ms
ab32124
Caspase 3 antibody [E87]
M
ICC/IF, IHC-P, IP, WB
Rb
Hu, Rt
ab32351
Cytochrome C antibody [EPR1327]
M
ICC/IF, IHC-P, IP, WB
Rb
Hu, Ms, Rt
EIF2S1 (phospho S51) antibody [E90]
M
Dot Blot, ICC/IF, IHC-Fr, IHC-P,
WB
Rb
Hu, Ms, Rt, Dm,
AGMk, Ncra
GRP78 BiP antibody [EPR4040(2)]
M
IHC-P, WB
Rb
Hu, Ms, Rt
ab108613
ab133504
ab32157
GRP94 antibody [EPR3988]
M
ICC/IF, IHC-P, WB
Rb
Hu, Ms
ab108606
IRE1 (phospho S724) antibody [EPR5253]
M
WB
Rb
Hu
ab124945
JNK1 + JNK2 + JNK3 (phospho T183+T183+T221)
antibody [EPR5693]
M
ICC/IF, IHC-P, IP, WB
Rb
Hu, Ms, Rt
ab124956
PPP1A antibody [EP1511Y]
M
Flow Cyt, ICC/IF, IHC-P, IP, WB
Rb
Hu, Ms, Rt
ab52619
TRAF2 antibody [EPR6048]
M
Flow Cyt, ICC/IF, IHC-P, IP, WB
Rb
Hu, Ms, Rt
ab126758
XBP1 antibody [EPR4086]
M
Flow Cyt, ICC/IF, IHC-P, WB
Rb
Hu
ab109221
Related assay kits
Assay kit
Sample type
Product code
CytoPainter ER Staining Kit - Green Fluorescence
Adherent cells, suspension cells
ab139481
CytoPainter ER Staining Kit - Red Fluorescence
Adherent cells, suspension cells
ab139482
Related biochemicals
Substance
Description
SP600125
JNK inhibitor
Product code
ab120065
TCS JNK 5a
Selective JNK2/3 inhibitor
ab120835
Z-D(OMe)E(OMe)VD(OMe)-FMK
Cell permeable caspase-3 inhibitor
ab120488
Z-VAD(OMe)-FMK
Cell permeable, irreversible pan-caspase inhibitor
ab120487
Z-VAD-FMK
Irreversible general caspase inhibitor
ab120382
Related proteins
Protein
Expression system
Applications
ATF6 protein (Tagged)
Wheat germ
ELISA, SDS-PAGE, WB
Caspase 3 peptide
Blocking
Product code
ab131846
ab38283
NFAT5 protein
Wheat germ
ELISA, SDS-PAGE,
WB, other
ab112396
PERK protein (Active)
E. coli
Functional Studies,
SDS-PAGE, WB
ab101115
Secondary antibodies
Why choose Alexa Fluor® conjugated secondaries from Abcam?
• Tested extensively in our laboratories - guarantee bright staining and low background
• Large range of pre-adsorbed antibodies - ensure low species cross-reactivity
Immunohistochemical analysis of paraffin-embedded
human lung carcinoma tissue using ab45178 at 1/10
dilution.
ASK1 (MEKK5) antibody [EP553Y]
(ab45178)
star reviewed: Good
ASK1 is a component of a protein kinase
signal transduction cascade. It phosphorylates
and activates MAP2K4 and MAP2K6, which
in turn activate the JNK and p38 MAP
kinases respectively.
Clonality
M
Application
Flow Cyt, ICC/IF, IHC-P, WB
Host
Rb
Species reactivity
Hu, Ms
• Dilution range of 1/200 -1/1000 (10-2 µg/ml) in ICC/IF - perform at least 250 stainings
• Antibodies conjugated to Alexa Fluor®488/550/594/647 - for your multi-color imaging experiments
EXT_002_13
Key: AGMk - African Green Monkey, Dm - Fruit fly (Drosophila melanogaster), Hu - Human, Ms - Mouse, Ncra - Neurospora crassa, Rb - Rabbit, Rt - Rat
- Rabbit Monoclonal Antibodies (RabMAbs)
Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.
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