Overview
Product name
Description
Tested applications
Species reactivity
Immunogen
Positive control
General notes
Anti-Pentraxin 3 antibody [EPR6699]
Rabbit monoclonal [EPR6699] to Pentraxin 3
WB, Flow Cyt
Reacts with: Mouse, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
HL-60 treated with Dexamethasone, HL-60, Human spleen, NIH/3T3, and HUVEC lysates;
HUVEC cells
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
Form
Storage instructions
Dissociation constant (K
D
)
Liquid
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Stable for 12 months at -
20°C.
K
D
= 7.80 x 10
-11
M
Storage buffer
Learn more about K
D pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
1

Purity
Clonality
Clone number
Isotype
Protein A purified
Monoclonal
EPR6699
IgG
Applications
Our Abpromise guarantee covers the use of ab125007 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular
Flow Cyt
Application notes weight: 42 kDa).
1/10 - 1/100. ab172730 -Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for ICC or IHC-P.
Target
Function
Sequence similarities
Post-translational modifications
Cellular localization
Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.
Contains 1 pentaxin domain.
Glycosylated.
Secreted.
Anti-Pentraxin 3 antibody [EPR6699] images
Overlay histogram showing HUVEC cells stained with ab125007 (red line) at 1/40 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit
IgG at 1/150 dilution. Isotype control antibody
(black line) was rabbit monoclonal IgG used under the same conditions.
Flow Cytometry - Anti-Pentraxin 3 antibody
[EPR6699] (ab125007)
2

Anti-Pentraxin 3 antibody [EPR6699]
(ab125007) at 1/10000 dilution + NIH/3T3 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Predicted band size : 42 kDa
Western blot - Anti-Pentraxin 3 antibody
[EPR6699] (ab125007)
Anti-Pentraxin 3 antibody [EPR6699]
(ab125007) at 1/10000 dilution + HUVEC cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution
Predicted band size : 42 kDa
Western blot - Anti-Pentraxin 3 antibody
[EPR6699] (ab125007)
3

Western blot - Anti-Pentraxin 3 antibody
[EPR6699] (ab125007)
All lanes : Anti-Pentraxin 3 antibody
[EPR6699] (ab125007) at 1/1000 dilution
(unpurified)
Lane 1 : HL-60 treated with Dexamethasone lysate
Lane 2 : HL-60 lysate
Lane 3 : Human spleen lysate
Lane 4 : NIH/3T3 cell lysate
Lane 5 : HUVEC lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 42 kDa
Observed band size : 42 kDa
Flow cytometric analysis of permeabilized
HUVEC cells using ab125007, unpurified, at
1/10 dilution (red) or a rabbit IgG (negative)
(green).
Flow Cytometry - Anti-Pentraxin 3 antibody
[EPR6699] (ab125007)
Equilibrium disassociation constant (K
D
)
Learn more about K
D
Click here to learn more about K
D
Other-Anti-Pentraxin 3 antibody [EPR6699]
(ab125007)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
4

Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
5