Literature Building Mold Sampling Report University of California, San Diego Prepared By: Aurora Industrial Hygiene San Diego, CA Date: April 6, 2009 Prepared By: Karen G. Shockley, CIH #6766 TABLE OF CONTENTS USE OF THIS REPORT ............................................................................................................................................. 3 1.0 Introduction ..................................................................................................................................................... 3 2.0 Executive Summary ......................................................................................................................................... 3 3.0 Observations..................................................................................................................................................... 4 Table 1. Room Observations .............................................................................................................................. 4 4.0 Mold Sampling Methodology.......................................................................................................................... 5 Total Spore Air Samples............................................................................................................................................ 6 Viable Air Samples .................................................................................................................................................... 6 Moisture Readings ..................................................................................................................................................... 6 5.0 Laboratory Results for Mold Sampling ......................................................................................................... 7 Table 2. Indoor and Outdoor Total Spore Air Sampling Results – March 5, 2009 ...................................... 8 Table 3. Indoor and Outdoor Viable Spore Air Sampling Results – March 5, 2009 .................................... 9 Table 4. Indoor Total Spore Air Sampling Results – 4th Floor ..................................................................... 10 Table 5. Indoor Total Spore Air Sampling Results – 3rd Floor ..................................................................... 11 Table 6. Indoor Total Spore Air Sampling Results – 2nd & 1st Floors .......................................................... 12 Table 7. Indoor Total Spore Air Sampling Results – 1st Floor...................................................................... 13 Table 8. Outdoor Total Spore Air Sampling Results – March 10 and 12, 2009 .......................................... 14 6.0 Mold Discussion ............................................................................................................................................. 15 7.0 Limitations ..................................................................................................................................................... 16 Appendix One – Photographs ................................................................................................................................... 17 Appendix Two – Site Diagrams ................................................................................................................................ 27 Appendix Three – Laboratory Reports of Analysis and Chains of Custody ........................................................ 31 Literature Building University of California, San Diego Page 2 April 2009 USE OF THIS REPORT This report is intended to provide an understanding of the potential hazards that the property evaluated in this report may pose to human health due to airborne mold. This report is based primarily upon data and information obtained during a three site visits by Aurora Industrial Hygiene, Inc. (Aurora) to the property identified herein on March 5, 10, and 12, 2009 and is based solely upon the condition of the property on the dates of such assessments. Aurora has performed the work, made the findings, and proposed recommendations described in this report in accordance with generally accepted industrial hygiene and environmental science practices for mold in effect at the time the work was performed. This warrantee stands in lieu of all other warranties, expressed or implied. While this report can be used as a guide by the client, it must be understood that changing circumstances in the environment and in property usage can alter radically the conclusions and information contained in this report. 1.0 Introduction This report documents the findings from mold sampling conducted by Aurora in the Literature Building (#627) on the UCSD La Jolla Campus in San Diego, California. The purposes of the survey were to visually assess the condition of the rooms sampled for signs of water damage or intrusion, and to conduct sampling to indicate whether mold was compromising the indoor air quality in selected rooms throughout all four floors of the building. The survey was limited to a list of rooms provided by UCSD personnel. Per the scope of work as outlined by UCSD, the remainder of the Literature Building was not assessed. In the course of completing this inspection, Aurora met with Steve Benedict and Sarah Meyer, UCSD Environment, Health and Safety Department; and Nancy Ho-Wu and Nancy Daly, Literature Department. Karen Shockley and Rick Shockley visited the site on March 5, 2009. Rick Shockley returned to the site on March 10 and 12, 2009 to complete the inspection and collect samples. All work was completed under the supervision of Certified Industrial Hygienist Karen Shockley (CIH #6766). 2.0 Executive Summary Aurora was provided a list of rooms by UCSD. In each room, Aurora conducted a visual assessment and air sampling for mold. Moisture measurements were conducted if there was a visual indication of water intrusion and randomly of carpet and drywall walls in some of the rooms that were assessed. Air samples for total mold spores and viable mold spores were collected in the building and outdoors. The outdoor air samples were collected to provide an indication of background levels of airborne mold in the area. Surface tape lift samples for mold were not collected because no visible suspect mold was observed by Mr. Shockley. Literature Building University of California, San Diego Page 3 April 2009 Air sample results indicated that the airborne mold spores indoors were comparable to the outdoor/background sample locations. Results did not indicate any degradation of the air quality from mold. Moisture readings were all in the “safe/dry” range. 3.0 Observations Aurora conducted a visual assessment of the rooms on the list provided by UCSD. Aurora conducted moisture measurements if there was a visual indication of water intrusion and randomly of carpet and drywall walls in some of the rooms that were assessed. The visual assessment included looking above drop ceiling tiles only if stained tiles were noted. The following table indicates the number of operable windows, whether they were opened or closed during the assessment, and any other notable observations found by Mr. Shockley. Surface tape lift samples for mold were not collected because visible suspect mold was not observed by Mr. Shockley. Table 1. Room Observations Room # Number of Operable Window – Closed or Open 454 453 452 451 448 None 1 – Slightly open 1 – Closed 1 – Closed 1 – Closed 445 1 – Open 443 438 434 431 427 425 1 – Closed 1 – Open 1 – Closed 1 – Closed 1 – Closed 1 – Closed 410 None New office space 354 353 349 348 346 None 1 – Closed 1 – Closed 1 – Closed 1 – Closed Sink in the room 341 1 – Slightly open 337 None Literature Building University of California, San Diego Other Comments Office is dusty Could be a dog routinely in room, photo on shelf and bowl on floor Office is messy, dog food in room Tea cup and honey on a table Not used, pretty empty Office is messy, stain on carpet and wall near door appear to be from coffee Page 4 April 2009 Room # Number of Operable Window – Closed or Open 336 333 332 329 328 326 324 310 None 1 – Open 1 – Open 1 – Closed 1 – Closed 1 – Closed 1 – Closed None 254 None 251 242 240 223 1 – Closed 1 – Closed 1 – Slightly open None 210 None 110 116 117 123 124 128 129 130 133 134 137 138 155 139 140 141 150 None 1 – Closed 1 – Closed 1 – Closed 1 – Closed 1 – Closed 1 – Closed 1 – Closed 1 – Closed 1 – Closed Double door – Closed Double door – Open 2 Double doors – Closed 1 – Closed 1 – Closed 1 – Closed 1 – Closed 4.0 Other Comments Recent remodel (18 months), sink/kitchenette in area Sink in room, water staining on ceiling tile above sink but everything looked dry above it Fan on desk turned off prior to sampling There is a kitchen area with microwave and there was a food odor during sampling Dirty on top of filing cabinets Computer server room Sink in room Refrigerator and microwave in room Mold Sampling Methodology On March 5, 2009 Mr. Shockley collected air samples from ten sample locations, eight inside the building (two on each floor) and two outside of the building. On March 10, 2009 Mr. Shockley collected air samples from 45 sample locations, 42 inside the building (several on each floor) and Literature Building University of California, San Diego Page 5 April 2009 three outside of the building. On March 12, 2009 Mr. Shockley collected air samples from two sample locations, one inside room 242, which was inaccessible on March 10, 2009, and one outside of the building. The outdoor air samples were collected to provide an indication of background levels of airborne mold in the area. At each of the sample locations on March 5, one air sample was collected to assess total airborne spores and one was collected to assess viable airborne spores. On March 10 and 12, only air sampling for total mold spores was conducted. Surface tape lift samples for mold were not collected because visible suspect mold was not observed by Mr. Shockley. Moisture measurements were collected randomly of carpet and drywall walls in some of the rooms that were assessed and all of the readings were in the “Safe/Dry” range. Total Spore Air Samples Air-O-Cell spore trap 37-mm cassettes are used to assess total spores. A sampling pump calibrated to a flow rate of 15 liters per minute draws a known volume of air over a sampling period of up to five (5) minutes. Once collected, the sample is submitted to a microbial laboratory where a small glass slide is removed from the cassette for direct examination. Viable Air Samples Air sampling for viable airborne mold spores and bacteria is performed using the Aerotech A-6 Single Stage Microbial Sampler, through which a known volume of air is collected and recorded. A sampling pump calibrated to a flow rate of 28.3 liters per minute is used to draw air through the A-6 sampling device. Within the A-6 sampling device is a petri dish that contains a laboratory prepared agar gel. Airborne particulates are deposited onto the agar gel. The sample is then sealed and submitted to a microbial laboratory where it is incubated in a controlled environment for 7-14 days. The petri dish samples are then assessed for viable mold and/or bacteria colonies, and reported in colony forming units, or CFUs. All of the collected mold samples were assigned a unique sample identification number, placed in sealed containers, labeled, and hand-delivered by Aurora Industrial Hygiene personnel to EMLab P&K Laboratory (EMLab), located at 9089 Clairemont Mesa Blvd, Suite 106, San Diego, CA 92123. EMLab is accredited by the American Industrial Hygiene Association for microbiological analyses. Moisture Readings Moisture readings were collected using a Protometerī¤ penetrating moisture meter. In wood, the instrument measures the material’s actual percent moisture content (%H2O). When testing material other than wood, the meter measures the wood moisture equivalent (WME) value of the material. WME is the moisture level that would be attained by a piece of wood in equilibrium with the material being tested. As the critical moisture levels for wood are known, WME measurements enable the moisture meter user to establish if materials are in a safe air dry, borderline or damp condition. <18% (Green Zone) The material is in a Safe Dry condition, moisture related problems of decay/deterioration should not occur. Literature Building University of California, San Diego Page 6 April 2009 18 – 20% (Yellow Zone) The material is in a Borderline condition, decay/deterioration may occur under certain conditions. >20% (Red Zone) The material is in a Wet condition, decay/deterioration is inevitable in time unless the moisture level of the material is reduced. 5.0 Laboratory Results for Mold Sampling Tables 2, 3, 4, 5, 6, 7 and 8 summarize the laboratory results for mold air sampling. Photographs of some of the sample locations are included in Appendix One, a sample location diagram is included in Appendix Two and laboratory reports of analysis are included in Appendix Three. Literature Building University of California, San Diego Page 7 April 2009 Table 2. Indoor and Outdoor Total Spore Air Sampling Results – March 5, 2009 Sample Identification Fungi Alternaria Ascospores Basidiospores Cladosporium Other brown Penicillium/ Aspergillus types Pithomyces Rusts Smuts, Periconia, Myxomycetes 0305-01A Outdoors, West of Building 0305-02A Room 134 0305-03A Room 110 0305-04A Room 223 0305-05A Room 254 0305-06A Room 310 0305-07A Room 349 0305-08A Room 427 0305-09A Room 438 0305-10A Outdoors, South of Building Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 53 13 53 13 13 13 53 110 80 93 53 13 13 53 13 13 53 13 13 13 53 160 13 27 53 13 13 13 27 13 13 Background Debris1 1+ 2+ 2+ 2+ 2+ 2+ 2+ 2+ 2+ 1+ Sample Volume(liters) 75 75 75 75 75 75 75 75 75 75 Spores/ m3 250 120 13 120 80 80 13 250 40 170 Ulocladium 1 Background debris is an indication of the amount of non-biological particulate matter present on the slide (dust in the air) and is graded from 1+ to 4+ with 4+ indicating the largest amounts. To evaluate dust levels it is important to account for differences in sample volume. This background material is also an indication of visibility for the analyst and resultant difficulty reading the slide. For example, high background debris may obscure the small spores such as Penicillium/ Aspergillus group. Counts from areas with 4+ background debris should be regarded as minimal counts and may actually be higher than reported. Literature Building University of California, San Diego Page 8 April 2009 Table 3. Indoor and Outdoor Viable Spore Air Sampling Results – March 5, 2009 Sample Identification 0305-01B Outdoors, West of Building 0305-02B Room 134 0305-03B Room 110 0305-04B Room 223 0305-05B Room 254 0305-06B Room 310 0305-07B Room 349 0305-08B Room 427 0305-09B Room 438 0305-10B Outdoors, South of Building Fungi CFU2/m3 CFU/m3 CFU/m3 CFU/m3 CFU/m3 CFU/m3 CFU/m3 CFU/m3 CFU/m3 CFU/m3 12 Aureobasidium Cladosporium 47 24 Non-sporulating fungi Penicillium 24 Yeasts 12 Sample Volume(liters) CFU/ m3 2 12 82 12 12 12 24 24 130 12 24 12 12 59 84.9 84.9 84.9 84.9 84.9 84.9 84.9 84.9 84.9 84.9 82 24 24 120 12 <12 <12 35 59 190 CFU = colony forming units Literature Building University of California, San Diego Page 9 April 2009 Table 4. Indoor Total Spore Air Sampling Results – 4th Floor Sample Identification 0310-02 Room 454 0310-03 Room 453 0310-04 Room 452 0310-05 Room 451 0310-06 Room 448 0310-07 Room 445 0310-08 Room 443 0310-09 Room 434 0310-10 Room 431 0310-11 Room 425 0310-12 Room 410 Fungi Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 13 27 53 53 40 110 13 110 53 Alternaria Ascospores Basidiospores Cladosporium Other brown Penicillium/ Aspergillus types Pithomyces Rusts 13 53 53 13 53 53 27 53 53 53 13 Smuts, Periconia, Myxomycetes 13 Ulocladium Background Debris3 2+ 2+ 2+ 2+ 2+ 2+ 2+ 2+ 2+ 2+ 2+ Sample Volume(liters) 75 75 75 75 75 75 75 75 75 75 75 Spores/ m3 53 120 67 80 53 80 200 <13 110 160 <13 3 Background debris is an indication of the amount of non-biological particulate matter present on the slide (dust in the air) and is graded from 1+ to 4+ with 4+ indicating the largest amounts. To evaluate dust levels it is important to account for differences in sample volume. This background material is also an indication of visibility for the analyst and resultant difficulty reading the slide. For example, high background debris may obscure the small spores such as Penicillium/ Aspergillus group. Counts from areas with 4+ background debris should be regarded as minimal counts and may actually be higher than reported. Literature Building University of California, San Diego Page 10 April 2009 Table 5. Indoor Total Spore Air Sampling Results – 3rd Floor Sample Identification 0310-13 Room 354 0310-14 Room 353 0310-15 Room 348 0310-16 Room 346 0310-17 Room 341 0310-18 Room 337 0310-19 Room 336 0310-20 Room 333 0310-21 Room 332 0310-22 Room 329 0310-23 Room 328 0310-24 Room 326 0310-25 Room 324 Fungi Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Alternaria Basidiospores Bipolaris/ Drechlera group Cladosporium Other brown Penicillium/ Aspergillus types Rusts Smuts, Periconia, Myxomycetes Stachybotrys Torula Trichocladium Background Debris4 Sample Volume (liters) Spores/ m3 13 13 13 13 13 13 13 13 53 53 27 160 13 53 53 27 53 13 27 53 13 13 27 13 13 13 13 13 2+ 2+ 2+ 3+ 2+ 2+ 2+ 2+ 2+ 2+ 3+ 2+ 2+ 75 75 75 75 75 75 75 75 75 75 75 75 75 27 93 130 190 110 27 13 27 67 27 80 13 13 4 Background debris is an indication of the amount of non-biological particulate matter present on the slide (dust in the air) and is graded from 1+ to 4+ with 4+ indicating the largest amounts. To evaluate dust levels it is important to account for differences in sample volume. This background material is also an indication of visibility for the analyst and resultant difficulty reading the slide. For example, high background debris may obscure the small spores such as Penicillium/ Aspergillus group. Counts from areas with 4+ background debris should be regarded as minimal counts and may actually be higher than reported. Literature Building University of California, San Diego Page 11 April 2009 Table 6. Indoor Total Spore Air Sampling Results – 2nd & 1st Floors Sample Identification 0310-26 Room 251 0310-27 Room 240 0312-01 Room 242 0310-28 Room 210 0310-30 Room 116 0310-31 Room 117 0310-32 Room 123 0310-33 Room 124 0310-34 Room 128 Fungi Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Alternaria Ascospores Basidiospores 13 40 80 13 Bipolaris/ Drechlera group Cladosporium Epicoccum Other brown Penicillium/ Aspergillus types Rusts Smuts, Periconia, Myxomycetes 53 110 320 53 13 110 160 13 13 53 13 13 110 53 110 53 53 13 13 13 Background Debris5 2+ 3+ 3+ 2+ 3+ 2+ 3+ 2+ 2+ Sample Volume(liters) 75 75 75 75 75 75 75 75 75 Spores/ m3 110 320 350 110 230 53 190 67 67 Ulocladium 5 Background debris is an indication of the amount of non-biological particulate matter present on the slide (dust in the air) and is graded from 1+ to 4+ with 4+ indicating the largest amounts. To evaluate dust levels it is important to account for differences in sample volume. This background material is also an indication of visibility for the analyst and resultant difficulty reading the slide. For example, high background debris may obscure the small spores such as Penicillium/ Aspergillus group. Counts from areas with 4+ background debris should be regarded as minimal counts and may actually be higher than reported. Literature Building University of California, San Diego Page 12 April 2009 Table 7. Indoor Total Spore Air Sampling Results – 1st Floor Sample Identification 0310-35 Room 129 0310-36 Room 130 0310-37 Room 133 0310-38 Room 137 0310-39 Room 138 0310-40 Room 155 0310-41 Room 139 0310-42 Room 140 0310-43 Room 141 0310-44 Room 150 Fungi Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 Spores/m3 13 13 Alternaria Ascospores Basidiospores Cladosporium Curvularia Epicoccum Other brown Penicillium/ Aspergillus types Rusts 13 53 13 53 13 53 Smuts, Periconia, Myxomycetes 53 53 53 13 13 13 13 13 53 53 13 Ulocladium Background Debris6 2+ 2+ 2+ 2+ 2+ 2+ 2+ 3+ 2+ 2+ Sample Volume(liters) 75 75 75 75 75 75 75 75 75 75 Spores/ m3 <13 67 110 130 27 13 67 130 <13 27 6 Background debris is an indication of the amount of non-biological particulate matter present on the slide (dust in the air) and is graded from 1+ to 4+ with 4+ indicating the largest amounts. To evaluate dust levels it is important to account for differences in sample volume. This background material is also an indication of visibility for the analyst and resultant difficulty reading the slide. For example, high background debris may obscure the small spores such as Penicillium/ Aspergillus group. Counts from areas with 4+ background debris should be regarded as minimal counts and may actually be higher than reported. Literature Building University of California, San Diego Page 13 April 2009 Table 8. Outdoor Total Spore Air Sampling Results – March 10 and 12, 2009 Sample Identification Fungi Alternaria Ascospores Basidiospores 0310-01 Outdoors, East of building 0310-29 Outdoors, West of building 0310-45 Outdoors, South of building 0312-02 Outdoors, South of building Spores/m3 Spores/m3 Spores/m3 Spores/m3 13 53 93 40 67 110 53 160 53 Bipolaris/ Drechlera group Cladosporium Curvularia Epicoccum Other brown Penicillium/ Aspergillus types 27 450 53 Pithomyces Rusts Smuts, Periconia, Myxomycetes Stachybotrys Torula Trichocladium Ulocladium 7 Background Debris7 2+ 2+ 2+ 1+ Sample Volume(liters) 75 75 75 75 Spores/ m3 160 640 250 120 Background debris is an indication of the amount of non-biological particulate matter present on the slide (dust in the air) and is graded from 1+ to 4+ with 4+ indicating the largest amounts. To evaluate dust levels it is important to account for differences in sample volume. This background material is also an indication of visibility for the analyst and resultant difficulty reading the slide. For example, high background debris may obscure the small spores such as Penicillium/ Aspergillus group. Counts from areas with 4+ background debris should be regarded as minimal counts and may actually be higher than reported. Literature Building Page 14 University of California, San Diego April 2009 6.0 Mold Discussion No regulatory standards exist for air or surface mold spores. High variability in mold spore concentrations will exist in different geographic locations, seasons, local weather patterns, and on a diurnal basis. The following basic guide provided by ETA Laboratories was used as a reference. Typical Indoor Mold Spore Concentration Ranges Description Spores /m3 "Clean" building Less than 2,000 Less than 700 Less than 500 Predominant Types Total for all spore types Penicillium, Aspergillus "Outdoor spores": alternaria, ascospores, basidiospores, cladosporium Possible Indoor Amplification 1,000 - 5,000 Penicillium, Aspergillus, Cladosporium Indoor amplification present 5,000 - 10,000 Penicillium, Aspergillus, Cladosporium Chronic Indoor Amplification 10,000 6,000,000 Penicillium, Aspergillus, Cladosporium, Stachybotrys, Basidiospores As a general rule, indoor fungal problems are usually indicated when a significant difference is demonstrated between indoor and outdoor airborne spore concentrations or types of spore genera or species. Mold spores concentrations indoors were comparable to outdoor samples and results did not indicate an indoor amplification of mold. Surface tape lift samples for mold were not collected because visible suspect mold was not observed by Mr. Shockley. Moisture measurements were collected randomly of carpet and drywall walls in some of the rooms that were inspected and all of the readings were in the “Safe/Dry” range. Literature Building University of California, San Diego Page 15 April 2009 7.0 Limitations The data and observations collected during the course of this assessment have been gathered to provide the Client with information pertaining to the areas of the subject property identified in this report. Although Aurora believes that the findings and conclusions provided in this report are reasonable, the assessment is limited to the conditions observed and to the information available at the time of the work. Due to the nature of the work, there is a possibility that there may exist conditions which could not be identified within the scope of the assessment or which were not apparent at the time of our site work. The assessment is also limited to information available from the client at the time it was conducted. It is also possible that the testing methods employed at the time of the report may later be superceded by other methods. Aurora does not accept responsibility for changes in the state of the art. Aurora does not guarantee that all mold contaminated areas in the subject property were recognized during our evaluation. This report is limited to the samples taken and locations sampled. Additional sampling may be needed to further identify other pollutants or other mold contaminated areas at the subject property. Microbial growth may occur if sources of moisture are not remediated. We hope that this information is helpful. Please feel free to contact us at (619) 276-5901 if you have any questions. Literature Building University of California, San Diego Page 16 April 2009 Appendix One – Photographs Interstate 805 Interstate 5 Photo One – Satellite photo. The dashed box is around the section of the UCSD La Jolla Campus that includes the Literature Building. Literature Building University of California, San Diego Page 17 April 2009 Genesee Avenue Interstate 5 Photo Two – Another satellite photo. The red arrow indicates the Literature Building. Literature Building University of California, San Diego Page 18 April 2009 Sample location west of the building Sample location east of the building Sample location south of the building Photo Three – Close-up photo of the Literature Building. The locations of the outdoor samples are indicated. Literature Building University of California, San Diego Page 19 April 2009 Photo Four – Outdoor sample location east of the building. Photo Five – Outdoor sample location south of the building. Literature Building University of California, San Diego Page 20 April 2009 Photo Six – Outdoor sample location west of the building. Photo Seven – Samples 0305-09A and 09B collected in room 438. The red arrow points towards a water dish and cup of dog food found in the room. Literature Building University of California, San Diego Page 21 April 2009 Photo Eight – Sample 0310-08 collected in room 434. The red arrow points to the tea cup and honey on the table. Photo Nine – Samples 0305-06A and 06B collected in room 310. Literature Building University of California, San Diego Page 22 April 2009 Photo Ten – Sample 0310-17 collected in room 341. Photo Eleven – Staining on carpet and wall in room 341 that appear to be from coffee. Literature Building University of California, San Diego Page 23 April 2009 Photo Twelve – Samples 0305-05A and 05B collected in room 254. Photo Thirteen – Staining on ceiling tile in room 254, everything looked dry above the stain. Literature Building University of California, San Diego Page 24 April 2009 Photo Fourteen – Sample 0310-28 collected in room 210. Photo Fifteen – Samples 0305-02A and 02B collected in room 134. Literature Building University of California, San Diego Page 25 April 2009 Photo Sixteen – Sample 0310-32 collected in room 123. Literature Building University of California, San Diego Page 26 April 2009 Appendix Two – Site Diagrams DIAGRAM NOT TO SCALE 0310-30 0310-33 0305-03 0310-31 0310-29 0305-01 0310-32 0310-01 0310-34 0310-40 0305-02 0310-35 0310-41 0310-37 0310-36 0310-44 0310-38 0310-39 0310-42 0310-45 0305-10 0310-43 0312-02 Literature Building University of California, San Diego Page 27 April 2009 0310-28 0305-04 0305-05 0310-26 0310-27 0312-01 Literature Building University of California, San Diego Page 28 April 2009 0310-25 0305-06 0310-24 0310-19 0310-13 0310-14 0310-22 0310-20 0310-23 0310-21 0310-18 0305-07 0310-17 0310-15 0310-16 Literature Building University of California, San Diego Page 29 April 2009 0310-12 0310-11 0305-08 0310-02 0310-09 0310-03 0310-04 0310-10 0305-09 0310-05 0310-06 0310-08 0310-07 Literature Building University of California, San Diego Page 30 April 2009 Appendix Three – Laboratory Reports of Analysis and Chains of Custody Literature Building University of California, San Diego Page 31 April 2009 EMLab P&K Report for: Ms. Karen Shockley Aurora Industrial Hygiene, Inc. 1094 Cudahy Place Suite 120 San Diego, CA 92110 Regarding: Project: UCSD-Literature Building EML ID: 519266 Approved by: Dates of Analysis: Culturable air fungi (Incl. Asp spp.): 03-12-2009 Spore trap analysis: 03-09-2009 Lab Manager Malcolm Moody Project SOPs: Culturable air fungi (Incl. Asp spp.) (I100002), Spore trap analysis (I100000) This coversheet is included with your report in order to comply with AIHA and ISO accreditation requirements. For clarity, we report the number of significant digits as calculated; but, due to the nature of this type of biological data, the number of significant digits that is used for interpretation should generally be one or two. All samples were received in acceptable condition unless noted in the Report Comments portion in the body of the report. Due to the nature of the analyses performed, field blank corrections of results is not a standard practice. The results relate only to the items tested. EMLab P&K ("the Company") shall have no liability to the client or the client's customer with respect to decisions or recommendations made, actions taken or courses of conduct implemented by either the client or the client's customer as a result of or based upon the Test Results. In no event shall the Company be liable to the client with respect to the Test Results except for the Company's own willful misconduct or gross negligence nor shall the Company be liable for incidental or consequential damages or lost profits or revenues to the fullest extent such liability may be disclaimed by law, even if the Company has been advised of the possibility of such damages, lost profits or lost revenues. In no event shall the Company's liability with respect to the Test Results exceed the amount paid to the Company by the client therefor. Document Number: 200091 - Revision Number: 5 EMLab P&K 1010 N Central Avenue, Glendale, CA 91202 (866) 465-6653 Fax (858) 569-5806 www.emlab.com Date of Sampling: 03-05-2009 Date of Receipt: 03-05-2009 Date of Report: 03-09-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Building SPORE TRAP REPORT: NON-VIABLE METHODOLOGY Location: 0305-01A: Outdoors, near main entrance Comments (see below) Lab ID-Version‡: 0305-02A: Room 134 0305-03A: Room 110 0305-04A: Room 223 None None None None 2298722-1 2298723-1 2298724-1 2298725-1 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 Alternaria Arthrinium Ascospores* Aureobasidium Basidiospores* Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Myrothecium Nigrospora Other brown Other colorless Penicillium/Aspergillus types† Pithomyces Rusts* Smuts*, Periconia, Myxomycetes* 3 80 4 93 1 13 1 53 1 53 1 13 1 13 1 13 2 27 1 13 1 13 1 53 1 13 1 53 Stachybotrys Stemphylium Torula Ulocladium Zygomycetes Background debris (1-4+)†† 1+ 2+ 2+ 2+ Sample volume (liters) 75 75 75 75 § TOTAL SPORE/m3 250 120 13 120 Comments: * Most of these spore types are not seen with culturable methods (Andersen sampling), although some may appear as non-sporulating fungi. Most of the basidiospores are "mushroom" spores while the rusts and smuts are plant pathogens. † The spores of Aspergillus and Penicillium (and others such as Acremonium, Paecilomyces) are small and round with very few distinguishing characteristics. They cannot be differentiated by non-viable sampling methods. Also, some species with very small spores are easily missed, and may be undercounted. ††Background debris indicates the amount of non-biological particulate matter present on the trace (dust in the air) and the resulting visibility for the analyst. It is rated from 1+ (low) to 4+ (high). Counts from areas with 4+ background debris should be regarded as minimal counts and may be higher then reported. It is important to account for samples volumes when evaluating dust levels. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total Spores/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 519266, Page 1 of 3 EMLab P&K 1010 N Central Avenue, Glendale, CA 91202 (866) 465-6653 Fax (858) 569-5806 www.emlab.com Date of Sampling: 03-05-2009 Date of Receipt: 03-05-2009 Date of Report: 03-09-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Building SPORE TRAP REPORT: NON-VIABLE METHODOLOGY Location: 0305-05A: Room 254 0305-06A: Room 310 0305-07A: Room 349 0305-08A: Room 427 None None None None 2298726-1 2298727-1 2298728-1 2298729-1 Comments (see below) Lab ID-Version‡: raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 Alternaria Arthrinium Ascospores* Aureobasidium Basidiospores* Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Myrothecium Nigrospora Other brown Other colorless Penicillium/Aspergillus types† Pithomyces Rusts* 1 13 1 53 1 13 Smuts*, Periconia, Myxomycetes* 1 53 1 13 1 1 13 Stachybotrys Stemphylium Torula Ulocladium Zygomycetes Background debris (1-4+)†† 2+ 2+ 2+ Sample volume (liters) 75 75 75 § TOTAL SPORE/m3 80 13 80 3 160 1 13 1 53 1 1 13 13 2+ 75 13 250 Comments: * Most of these spore types are not seen with culturable methods (Andersen sampling), although some may appear as non-sporulating fungi. Most of the basidiospores are "mushroom" spores while the rusts and smuts are plant pathogens. † The spores of Aspergillus and Penicillium (and others such as Acremonium, Paecilomyces) are small and round with very few distinguishing characteristics. They cannot be differentiated by non-viable sampling methods. Also, some species with very small spores are easily missed, and may be undercounted. ††Background debris indicates the amount of non-biological particulate matter present on the trace (dust in the air) and the resulting visibility for the analyst. It is rated from 1+ (low) to 4+ (high). Counts from areas with 4+ background debris should be regarded as minimal counts and may be higher then reported. It is important to account for samples volumes when evaluating dust levels. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total Spores/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 519266, Page 2 of 3 EMLab P&K 1010 N Central Avenue, Glendale, CA 91202 (866) 465-6653 Fax (858) 569-5806 www.emlab.com Date of Sampling: 03-05-2009 Date of Receipt: 03-05-2009 Date of Report: 03-09-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Building SPORE TRAP REPORT: NON-VIABLE METHODOLOGY Location: Comments (see below) Lab ID-Version‡: raw ct. Alternaria Arthrinium Ascospores* Aureobasidium Basidiospores* Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Myrothecium Nigrospora Other brown Other colorless Penicillium/Aspergillus types† Pithomyces Rusts* 1 2 0305-09A: Room 428 0305-10A: Outdoors, near south end of bldg None None 2298730-1 2298731-1 spores/m3 13 raw ct. spores/m3 1 13 1 53 2 110 27 Smuts*, Periconia, Myxomycetes* Stachybotrys Stemphylium Torula Ulocladium Zygomycetes Background debris (1-4+)†† 2+ Sample volume (liters) 75 § TOTAL SPORE/m3 1+ 75 40 170 Comments: * Most of these spore types are not seen with culturable methods (Andersen sampling), although some may appear as non-sporulating fungi. Most of the basidiospores are "mushroom" spores while the rusts and smuts are plant pathogens. † The spores of Aspergillus and Penicillium (and others such as Acremonium, Paecilomyces) are small and round with very few distinguishing characteristics. They cannot be differentiated by non-viable sampling methods. Also, some species with very small spores are easily missed, and may be undercounted. ††Background debris indicates the amount of non-biological particulate matter present on the trace (dust in the air) and the resulting visibility for the analyst. It is rated from 1+ (low) to 4+ (high). Counts from areas with 4+ background debris should be regarded as minimal counts and may be higher then reported. It is important to account for samples volumes when evaluating dust levels. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total Spores/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 519266, Page 3 of 3 EMLab P&K 1150 Bayhill Drive, Suite 100, San Bruno, CA 94066 (866) 888-6653 Fax (650) 829-5852 www.emlab.com Date of Sampling: 03-05-2009 Date of Receipt: 03-05-2009 Date of Report: 03-12-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Building CULTURABLE AIR FUNGI REPORT Location: 0305-01B: Outdoors, near main entrance Comments (see below) Lab ID-Version‡: Acremonium Alternaria Aspergillus flavus Aspergillus fumigatus Aspergillus nidulans Aspergillus niger Aspergillus ochraceus Aspergillus versicolor Aureobasidium Basidiomycetes Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Non-sporulating fungi Paecilomyces Penicillium Phoma Rhizopus Stachybotrys chartarum Ulocladium Yeasts Positive Hole Sample volume (liters) 0305-03B: Room 110 0305-04B: Room 223 None None None None 2298712-1 2298713-1 2298714-1 2298715-1 raw ct. cfu*/m3 raw ct. cfu*/m3 raw ct. cfu*/m3 raw ct. cfu*/m3 4 47 2 24 1 12 7 82 1 12 1 12 2 24 2 24 1 12 400 84.9 § TOTAL CFU*/M3 * cfu = colony forming units Comments: 0305-02B: Room 134 400 84.9 82 400 84.9 24 400 84.9 24 120 Positive hole correction chart used for all calculations Note: Interpretation is left to the company and/or persons who conducted the field work. Variation is an inherent part of biological sampling. The presence or absence of a few genera in small numbers should not be considered abnormal. NORMAL SPORE LEVELS: Indoor spore levels usually average 30 to 80% of the outdoor spore level at the time of sampling, with the same general distribution of spore types. Filtered air, air-conditioned air, or air remote from outside sources may average 5 to 15% of the outside air at the time of sampling. (These percentages are guidelines, only. A major factor is the accessibility of outdoor air. A residence with open doors and windows and heavy foot traffic may average 95% of the outdoor level while high rise office buildings with little air exchange may average 2%. Dusty interiors may exceed 100% of the outdoors to some degree, but will still mirror the outdoor distribution of spore types.) PROBLEM INTERIORS: A substantial increase of one or two spore types which are inconsistent with and non-reflective of the outside distribution of spore types is usually indicative of an indoor reservoir of mold growth. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total CFU/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 519266, Page 1 of 3 EMLab P&K 1150 Bayhill Drive, Suite 100, San Bruno, CA 94066 (866) 888-6653 Fax (650) 829-5852 www.emlab.com Date of Sampling: 03-05-2009 Date of Receipt: 03-05-2009 Date of Report: 03-12-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Building CULTURABLE AIR FUNGI REPORT Location: 0305-05B: Room 254 0305-06B: Room 310 0305-07B: Room 349 0305-08B: Room 427 None None None None 2298716-1 2298717-1 2298718-1 2298719-1 Comments (see below) Lab ID-Version‡: Acremonium Alternaria Aspergillus flavus Aspergillus fumigatus Aspergillus nidulans Aspergillus niger Aspergillus ochraceus Aspergillus versicolor Aureobasidium Basidiomycetes Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Non-sporulating fungi Paecilomyces Penicillium Phoma Rhizopus Stachybotrys chartarum Ulocladium Yeasts Positive Hole Sample volume (liters) raw ct. cfu*/m3 1 12 400 84.9 § TOTAL CFU*/M3 * cfu = colony forming units Comments: raw ct. cfu*/m3 400 84.9 12 raw ct. cfu*/m3 400 84.9 < 12 raw ct. cfu*/m3 2 24 1 12 400 84.9 < 12 35 Positive hole correction chart used for all calculations Note: Interpretation is left to the company and/or persons who conducted the field work. Variation is an inherent part of biological sampling. The presence or absence of a few genera in small numbers should not be considered abnormal. NORMAL SPORE LEVELS: Indoor spore levels usually average 30 to 80% of the outdoor spore level at the time of sampling, with the same general distribution of spore types. Filtered air, air-conditioned air, or air remote from outside sources may average 5 to 15% of the outside air at the time of sampling. (These percentages are guidelines, only. A major factor is the accessibility of outdoor air. A residence with open doors and windows and heavy foot traffic may average 95% of the outdoor level while high rise office buildings with little air exchange may average 2%. Dusty interiors may exceed 100% of the outdoors to some degree, but will still mirror the outdoor distribution of spore types.) PROBLEM INTERIORS: A substantial increase of one or two spore types which are inconsistent with and non-reflective of the outside distribution of spore types is usually indicative of an indoor reservoir of mold growth. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total CFU/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 519266, Page 2 of 3 EMLab P&K 1150 Bayhill Drive, Suite 100, San Bruno, CA 94066 (866) 888-6653 Fax (650) 829-5852 www.emlab.com Date of Sampling: 03-05-2009 Date of Receipt: 03-05-2009 Date of Report: 03-12-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Building CULTURABLE AIR FUNGI REPORT Location: Comments (see below) Lab ID-Version‡: Acremonium Alternaria Aspergillus flavus Aspergillus fumigatus Aspergillus nidulans Aspergillus niger Aspergillus ochraceus Aspergillus versicolor Aureobasidium Basidiomycetes Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Non-sporulating fungi Paecilomyces Penicillium Phoma Rhizopus Stachybotrys chartarum Ulocladium Yeasts Positive Hole Sample volume (liters) 0305-10B: Outdoors, near south end of bldg None None 2298720-1 2298721-1 raw ct. cfu*/m3 1 12 2 24 1 12 1 12 400 84.9 § TOTAL CFU*/M3 * cfu = colony forming units Comments: 0305-09B: Room 428 raw ct. cfu*/m3 11 130 5 59 400 84.9 59 190 Positive hole correction chart used for all calculations Note: Interpretation is left to the company and/or persons who conducted the field work. Variation is an inherent part of biological sampling. The presence or absence of a few genera in small numbers should not be considered abnormal. NORMAL SPORE LEVELS: Indoor spore levels usually average 30 to 80% of the outdoor spore level at the time of sampling, with the same general distribution of spore types. Filtered air, air-conditioned air, or air remote from outside sources may average 5 to 15% of the outside air at the time of sampling. (These percentages are guidelines, only. A major factor is the accessibility of outdoor air. A residence with open doors and windows and heavy foot traffic may average 95% of the outdoor level while high rise office buildings with little air exchange may average 2%. Dusty interiors may exceed 100% of the outdoors to some degree, but will still mirror the outdoor distribution of spore types.) PROBLEM INTERIORS: A substantial increase of one or two spore types which are inconsistent with and non-reflective of the outside distribution of spore types is usually indicative of an indoor reservoir of mold growth. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total CFU/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 519266, Page 3 of 3 EMLab P&K Report for: Ms. Karen Shockley Aurora Industrial Hygiene, Inc. 1094 Cudahy Place Suite 120 San Diego, CA 92110 Regarding: Project: UCSD-Literature Bldg EML ID: 520993 Approved by: Dates of Analysis: Spore trap analysis: 03-13-2009 Lab Manager Malcolm Moody Project SOPs: Spore trap analysis (I100000) This coversheet is included with your report in order to comply with AIHA and ISO accreditation requirements. For clarity, we report the number of significant digits as calculated; but, due to the nature of this type of biological data, the number of significant digits that is used for interpretation should generally be one or two. All samples were received in acceptable condition unless noted in the Report Comments portion in the body of the report. Due to the nature of the analyses performed, field blank corrections of results is not a standard practice. The results relate only to the items tested. EMLab P&K ("the Company") shall have no liability to the client or the client's customer with respect to decisions or recommendations made, actions taken or courses of conduct implemented by either the client or the client's customer as a result of or based upon the Test Results. In no event shall the Company be liable to the client with respect to the Test Results except for the Company's own willful misconduct or gross negligence nor shall the Company be liable for incidental or consequential damages or lost profits or revenues to the fullest extent such liability may be disclaimed by law, even if the Company has been advised of the possibility of such damages, lost profits or lost revenues. In no event shall the Company's liability with respect to the Test Results exceed the amount paid to the Company by the client therefor. Document Number: 200091 - Revision Number: 5 EMLab P&K 1010 N Central Avenue, Glendale, CA 91202 (866) 465-6653 Fax (858) 569-5806 www.emlab.com Date of Sampling: 03-10-2009 Date of Receipt: 03-11-2009 Date of Report: 03-13-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Bldg SPORE TRAP REPORT: NON-VIABLE METHODOLOGY Location: 0310-01: Outdoors, east of bldg 0310-02: Room 454 0310-03: Room 453 0310-04: Room 452 0310-05: Room 451 None None None None None 2306287-1 2306288-1 2306289-1 2306290-1 2306291-1 Comments (see below) Lab ID-Version‡: raw ct. Alternaria Arthrinium Ascospores* Aureobasidium Basidiospores* Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Myrothecium Nigrospora Other brown Other colorless Penicillium/Aspergillus types† Pithomyces Rusts* spores/m3 1 53 2 110 raw ct. 1 spores/m3 53 raw ct. spores/m3 1 13 1 1 53 raw ct. spores/m3 1 13 1 53 raw ct. spores/m3 1 53 2 27 53 Smuts*, Periconia, Myxomycetes* Stachybotrys Stemphylium Torula Trichocladium Ulocladium Zygomycetes Background debris (1-4+)†† 2+ 2+ 2+ 2+ Sample volume (liters) 75 75 75 75 § TOTAL SPORE/m3 160 53 120 2+ 75 67 80 Comments: * Most of these spore types are not seen with culturable methods (Andersen sampling), although some may appear as non-sporulating fungi. Most of the basidiospores are "mushroom" spores while the rusts and smuts are plant pathogens. † The spores of Aspergillus and Penicillium (and others such as Acremonium, Paecilomyces) are small and round with very few distinguishing characteristics. They cannot be differentiated by non-viable sampling methods. Also, some species with very small spores are easily missed, and may be undercounted. ††Background debris indicates the amount of non-biological particulate matter present on the trace (dust in the air) and the resulting visibility for the analyst. It is rated from 1+ (low) to 4+ (high). Counts from areas with 4+ background debris should be regarded as minimal counts and may be higher then reported. It is important to account for samples volumes when evaluating dust levels. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total Spores/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 520993, Page 1 of 11 EMLab P&K 1010 N Central Avenue, Glendale, CA 91202 (866) 465-6653 Fax (858) 569-5806 www.emlab.com Date of Sampling: 03-10-2009 Date of Receipt: 03-11-2009 Date of Report: 03-13-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Bldg SPORE TRAP REPORT: NON-VIABLE METHODOLOGY Location: 0310-06: Room 448 0310-07: Room 445 0310-08: Room 443 0310-09: Room 434 None None None None 2306292-1 2306293-1 2306294-1 2306295-1 Comments (see below) Lab ID-Version‡: raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 Alternaria Arthrinium Ascospores* Aureobasidium Basidiospores* Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Myrothecium Nigrospora Other brown Other colorless Penicillium/Aspergillus types† Pithomyces Rusts* 1 1 13 53 1 53 1 13 Smuts*, Periconia, Myxomycetes* 2 27 1 53 2 110 1 13 Stachybotrys Stemphylium Torula Trichocladium Ulocladium Zygomycetes Background debris (1-4+)†† 2+ 2+ 2+ 2+ Sample volume (liters) 75 75 75 75 § TOTAL SPORE/m3 53 80 200 < 13 Comments: * Most of these spore types are not seen with culturable methods (Andersen sampling), although some may appear as non-sporulating fungi. Most of the basidiospores are "mushroom" spores while the rusts and smuts are plant pathogens. † The spores of Aspergillus and Penicillium (and others such as Acremonium, Paecilomyces) are small and round with very few distinguishing characteristics. They cannot be differentiated by non-viable sampling methods. Also, some species with very small spores are easily missed, and may be undercounted. ††Background debris indicates the amount of non-biological particulate matter present on the trace (dust in the air) and the resulting visibility for the analyst. It is rated from 1+ (low) to 4+ (high). Counts from areas with 4+ background debris should be regarded as minimal counts and may be higher then reported. It is important to account for samples volumes when evaluating dust levels. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total Spores/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 520993, Page 2 of 11 EMLab P&K 1010 N Central Avenue, Glendale, CA 91202 (866) 465-6653 Fax (858) 569-5806 www.emlab.com Date of Sampling: 03-10-2009 Date of Receipt: 03-11-2009 Date of Report: 03-13-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Bldg SPORE TRAP REPORT: NON-VIABLE METHODOLOGY Location: 0310-10: Room 431 0310-11: Room 425 0310-12: Room 410 0310-13: Room 354 None None None None 2306296-1 2306297-1 2306298-1 2306299-1 Comments (see below) Lab ID-Version‡: raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 Alternaria Arthrinium Ascospores* Aureobasidium Basidiospores* Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Myrothecium Nigrospora Other brown Other colorless Penicillium/Aspergillus types† Pithomyces Rusts* 1 1 53 3 40 2 110 1 13 1 13 1 13 53 Smuts*, Periconia, Myxomycetes* Stachybotrys Stemphylium Torula Trichocladium Ulocladium Zygomycetes Background debris (1-4+)†† 2+ 2+ 2+ 2+ Sample volume (liters) 75 75 75 75 § TOTAL SPORE/m3 110 160 < 13 27 Comments: * Most of these spore types are not seen with culturable methods (Andersen sampling), although some may appear as non-sporulating fungi. Most of the basidiospores are "mushroom" spores while the rusts and smuts are plant pathogens. † The spores of Aspergillus and Penicillium (and others such as Acremonium, Paecilomyces) are small and round with very few distinguishing characteristics. They cannot be differentiated by non-viable sampling methods. Also, some species with very small spores are easily missed, and may be undercounted. ††Background debris indicates the amount of non-biological particulate matter present on the trace (dust in the air) and the resulting visibility for the analyst. It is rated from 1+ (low) to 4+ (high). Counts from areas with 4+ background debris should be regarded as minimal counts and may be higher then reported. It is important to account for samples volumes when evaluating dust levels. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total Spores/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 520993, Page 3 of 11 EMLab P&K 1010 N Central Avenue, Glendale, CA 91202 (866) 465-6653 Fax (858) 569-5806 www.emlab.com Date of Sampling: 03-10-2009 Date of Receipt: 03-11-2009 Date of Report: 03-13-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Bldg SPORE TRAP REPORT: NON-VIABLE METHODOLOGY Location: 0310-14: Room 353 0310-15: Room 348 0310-16: Room 346 0310-17: Room 341 None None None None 2306300-1 2306301-1 2306302-1 2306303-1 Comments (see below) Lab ID-Version‡: raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 Alternaria Arthrinium Ascospores* Aureobasidium Basidiospores* Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Myrothecium Nigrospora Other brown Other colorless Penicillium/Aspergillus types† Pithomyces Rusts* Smuts*, Periconia, Myxomycetes* 1 1 2 13 53 1 13 1 13 1 13 1 53 3 160 1 53 2 27 1 13 2 27 1 53 27 Stachybotrys Stemphylium Torula Trichocladium Ulocladium Zygomycetes Background debris (1-4+)†† 2+ 2+ 3+ 2+ Sample volume (liters) 75 75 75 75 § TOTAL SPORE/m3 93 130 190 110 Comments: * Most of these spore types are not seen with culturable methods (Andersen sampling), although some may appear as non-sporulating fungi. Most of the basidiospores are "mushroom" spores while the rusts and smuts are plant pathogens. † The spores of Aspergillus and Penicillium (and others such as Acremonium, Paecilomyces) are small and round with very few distinguishing characteristics. They cannot be differentiated by non-viable sampling methods. Also, some species with very small spores are easily missed, and may be undercounted. ††Background debris indicates the amount of non-biological particulate matter present on the trace (dust in the air) and the resulting visibility for the analyst. It is rated from 1+ (low) to 4+ (high). Counts from areas with 4+ background debris should be regarded as minimal counts and may be higher then reported. It is important to account for samples volumes when evaluating dust levels. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total Spores/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 520993, Page 4 of 11 EMLab P&K 1010 N Central Avenue, Glendale, CA 91202 (866) 465-6653 Fax (858) 569-5806 www.emlab.com Date of Sampling: 03-10-2009 Date of Receipt: 03-11-2009 Date of Report: 03-13-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Bldg SPORE TRAP REPORT: NON-VIABLE METHODOLOGY Location: 0310-18: Room 337 0310-19: Room 336 0310-20: Room 333 0310-21: Room 332 None None None None 2306304-1 2306305-1 2306306-1 2306307-1 Comments (see below) Lab ID-Version‡: raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 Alternaria Arthrinium Ascospores* Aureobasidium Basidiospores* Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Myrothecium Nigrospora Other brown Other colorless Penicillium/Aspergillus types† Pithomyces Rusts* 1 1 1 13 1 13 1 53 13 13 Smuts*, Periconia, Myxomycetes* Stachybotrys Stemphylium Torula Trichocladium Ulocladium Zygomycetes Background debris (1-4+)†† 2+ 2+ 2+ 2+ Sample volume (liters) 75 75 75 75 § TOTAL SPORE/m3 1 13 1 13 27 13 27 67 Comments: * Most of these spore types are not seen with culturable methods (Andersen sampling), although some may appear as non-sporulating fungi. Most of the basidiospores are "mushroom" spores while the rusts and smuts are plant pathogens. † The spores of Aspergillus and Penicillium (and others such as Acremonium, Paecilomyces) are small and round with very few distinguishing characteristics. They cannot be differentiated by non-viable sampling methods. Also, some species with very small spores are easily missed, and may be undercounted. ††Background debris indicates the amount of non-biological particulate matter present on the trace (dust in the air) and the resulting visibility for the analyst. It is rated from 1+ (low) to 4+ (high). Counts from areas with 4+ background debris should be regarded as minimal counts and may be higher then reported. It is important to account for samples volumes when evaluating dust levels. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total Spores/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 520993, Page 5 of 11 EMLab P&K 1010 N Central Avenue, Glendale, CA 91202 (866) 465-6653 Fax (858) 569-5806 www.emlab.com Date of Sampling: 03-10-2009 Date of Receipt: 03-11-2009 Date of Report: 03-13-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Bldg SPORE TRAP REPORT: NON-VIABLE METHODOLOGY Location: 0310-22: Room 329 0310-23: Room 328 0310-24: Room 326 0310-25: Room 324 None None None None 2306308-1 2306309-1 2306310-1 2306311-1 Comments (see below) Lab ID-Version‡: raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 Alternaria Arthrinium Ascospores* Aureobasidium Basidiospores* Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Myrothecium Nigrospora Other brown Other colorless Penicillium/Aspergillus types† Pithomyces Rusts* Smuts*, Periconia, Myxomycetes* 1 1 13 1 13 1 53 2 27 13 Stachybotrys Stemphylium Torula Trichocladium Ulocladium Zygomycetes Background debris (1-4+)†† 2+ 3+ 2+ 2+ Sample volume (liters) 75 75 75 75 § TOTAL SPORE/m3 1 27 80 13 13 13 Comments: * Most of these spore types are not seen with culturable methods (Andersen sampling), although some may appear as non-sporulating fungi. Most of the basidiospores are "mushroom" spores while the rusts and smuts are plant pathogens. † The spores of Aspergillus and Penicillium (and others such as Acremonium, Paecilomyces) are small and round with very few distinguishing characteristics. They cannot be differentiated by non-viable sampling methods. Also, some species with very small spores are easily missed, and may be undercounted. ††Background debris indicates the amount of non-biological particulate matter present on the trace (dust in the air) and the resulting visibility for the analyst. It is rated from 1+ (low) to 4+ (high). Counts from areas with 4+ background debris should be regarded as minimal counts and may be higher then reported. It is important to account for samples volumes when evaluating dust levels. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total Spores/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 520993, Page 6 of 11 EMLab P&K 1010 N Central Avenue, Glendale, CA 91202 (866) 465-6653 Fax (858) 569-5806 www.emlab.com Date of Sampling: 03-10-2009 Date of Receipt: 03-11-2009 Date of Report: 03-13-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Bldg SPORE TRAP REPORT: NON-VIABLE METHODOLOGY Location: 0310-26: Room 251 0310-27: Room 240 0310-28: Room 210 0310-29: Outdoors, west of building None None None A 2306312-1 2306313-1 2306314-1 2306315-1 Comments (see below) Lab ID-Version‡: raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 Alternaria Arthrinium Ascospores* Aureobasidium Basidiospores* Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Myrothecium Nigrospora Other brown Other colorless Penicillium/Aspergillus types† Pithomyces Rusts* Smuts*, Periconia, Myxomycetes* 3 40 3 80 1 53 2 110 1 13 2 110 1 13 1 13 1 1 Stachybotrys Stemphylium Torula Trichocladium Ulocladium Zygomycetes Background debris (1-4+)†† 2+ 3+ 2+ Sample volume (liters) 75 75 75 § TOTAL SPORE/m3 110 320 53 53 1 13 4 93 1 53 2 27 31 450 2+ 75 110 640 Comments:A) 30 of the raw count Penicillium/Aspergillus type spores were present as a single clump. * Most of these spore types are not seen with culturable methods (Andersen sampling), although some may appear as non-sporulating fungi. Most of the basidiospores are "mushroom" spores while the rusts and smuts are plant pathogens. † The spores of Aspergillus and Penicillium (and others such as Acremonium, Paecilomyces) are small and round with very few distinguishing characteristics. They cannot be differentiated by non-viable sampling methods. Also, some species with very small spores are easily missed, and may be undercounted. ††Background debris indicates the amount of non-biological particulate matter present on the trace (dust in the air) and the resulting visibility for the analyst. It is rated from 1+ (low) to 4+ (high). Counts from areas with 4+ background debris should be regarded as minimal counts and may be higher then reported. It is important to account for samples volumes when evaluating dust levels. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total Spores/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 520993, Page 7 of 11 EMLab P&K 1010 N Central Avenue, Glendale, CA 91202 (866) 465-6653 Fax (858) 569-5806 www.emlab.com Date of Sampling: 03-10-2009 Date of Receipt: 03-11-2009 Date of Report: 03-13-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Bldg SPORE TRAP REPORT: NON-VIABLE METHODOLOGY Location: 0310-30: Room 116 0310-31: Room 117 0310-32: Room 123 0310-33: Room 124 None None None None 2306316-1 2306317-1 2306318-1 2306319-1 Comments (see below) Lab ID-Version‡: raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 Alternaria Arthrinium Ascospores* Aureobasidium Basidiospores* Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Myrothecium Nigrospora Other brown Other colorless Penicillium/Aspergillus types† Pithomyces Rusts* 2 110 1 13 2 110 1 3 160 1 13 1 13 1 13 1 53 53 Smuts*, Periconia, Myxomycetes* Stachybotrys Stemphylium Torula Trichocladium Ulocladium Zygomycetes Background debris (1-4+)†† 3+ 2+ 3+ 2+ Sample volume (liters) 75 75 75 75 § TOTAL SPORE/m3 230 53 190 67 Comments: * Most of these spore types are not seen with culturable methods (Andersen sampling), although some may appear as non-sporulating fungi. Most of the basidiospores are "mushroom" spores while the rusts and smuts are plant pathogens. † The spores of Aspergillus and Penicillium (and others such as Acremonium, Paecilomyces) are small and round with very few distinguishing characteristics. They cannot be differentiated by non-viable sampling methods. Also, some species with very small spores are easily missed, and may be undercounted. ††Background debris indicates the amount of non-biological particulate matter present on the trace (dust in the air) and the resulting visibility for the analyst. It is rated from 1+ (low) to 4+ (high). Counts from areas with 4+ background debris should be regarded as minimal counts and may be higher then reported. It is important to account for samples volumes when evaluating dust levels. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total Spores/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 520993, Page 8 of 11 EMLab P&K 1010 N Central Avenue, Glendale, CA 91202 (866) 465-6653 Fax (858) 569-5806 www.emlab.com Date of Sampling: 03-10-2009 Date of Receipt: 03-11-2009 Date of Report: 03-13-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Bldg SPORE TRAP REPORT: NON-VIABLE METHODOLOGY Location: 0310-34: Room 128 0310-35: Room 129 0310-36: Room 130 0310-37: Room 133 None None None None 2306320-1 2306321-1 2306322-1 2306323-1 Comments (see below) Lab ID-Version‡: raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 Alternaria Arthrinium Ascospores* Aureobasidium Basidiospores* Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Myrothecium Nigrospora Other brown Other colorless Penicillium/Aspergillus types† Pithomyces Rusts* 1 13 1 53 Smuts*, Periconia, Myxomycetes* 1 53 1 13 1 53 1 53 Stachybotrys Stemphylium Torula Trichocladium Ulocladium Zygomycetes Background debris (1-4+)†† 2+ 2+ 2+ 2+ Sample volume (liters) 75 75 75 75 § TOTAL SPORE/m3 67 < 13 67 110 Comments: * Most of these spore types are not seen with culturable methods (Andersen sampling), although some may appear as non-sporulating fungi. Most of the basidiospores are "mushroom" spores while the rusts and smuts are plant pathogens. † The spores of Aspergillus and Penicillium (and others such as Acremonium, Paecilomyces) are small and round with very few distinguishing characteristics. They cannot be differentiated by non-viable sampling methods. Also, some species with very small spores are easily missed, and may be undercounted. ††Background debris indicates the amount of non-biological particulate matter present on the trace (dust in the air) and the resulting visibility for the analyst. It is rated from 1+ (low) to 4+ (high). Counts from areas with 4+ background debris should be regarded as minimal counts and may be higher then reported. It is important to account for samples volumes when evaluating dust levels. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total Spores/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 520993, Page 9 of 11 EMLab P&K 1010 N Central Avenue, Glendale, CA 91202 (866) 465-6653 Fax (858) 569-5806 www.emlab.com Date of Sampling: 03-10-2009 Date of Receipt: 03-11-2009 Date of Report: 03-13-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Bldg SPORE TRAP REPORT: NON-VIABLE METHODOLOGY Location: 0310-38: Room 137 0310-39: Room 138 0310-40: Room 155 0310-41: Room 139 None None None None 2306324-1 2306325-1 2306326-1 2306327-1 Comments (see below) Lab ID-Version‡: raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 Alternaria Arthrinium Ascospores* Aureobasidium Basidiospores* Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Myrothecium Nigrospora Other brown Other colorless Penicillium/Aspergillus types† Pithomyces Rusts* 1 1 13 1 53 1 13 1 53 13 1 1 13 1 13 1 53 13 Smuts*, Periconia, Myxomycetes* Stachybotrys Stemphylium Torula Trichocladium Ulocladium Zygomycetes Background debris (1-4+)†† 2+ 2+ 2+ 2+ Sample volume (liters) 75 75 75 75 § TOTAL SPORE/m3 130 27 13 67 Comments: * Most of these spore types are not seen with culturable methods (Andersen sampling), although some may appear as non-sporulating fungi. Most of the basidiospores are "mushroom" spores while the rusts and smuts are plant pathogens. † The spores of Aspergillus and Penicillium (and others such as Acremonium, Paecilomyces) are small and round with very few distinguishing characteristics. They cannot be differentiated by non-viable sampling methods. Also, some species with very small spores are easily missed, and may be undercounted. ††Background debris indicates the amount of non-biological particulate matter present on the trace (dust in the air) and the resulting visibility for the analyst. It is rated from 1+ (low) to 4+ (high). Counts from areas with 4+ background debris should be regarded as minimal counts and may be higher then reported. It is important to account for samples volumes when evaluating dust levels. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total Spores/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 520993, Page 10 of 11 EMLab P&K 1010 N Central Avenue, Glendale, CA 91202 (866) 465-6653 Fax (858) 569-5806 www.emlab.com Date of Sampling: 03-10-2009 Date of Receipt: 03-11-2009 Date of Report: 03-13-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Bldg SPORE TRAP REPORT: NON-VIABLE METHODOLOGY Location: 0310-42: Room 140 0310-43: Room 141 0310-44: Room 150 0310-45: Outdoors, near SW corner None None None None 2306328-1 2306329-1 2306330-1 2306331-1 Comments (see below) Lab ID-Version‡: raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 raw ct. spores/m3 Alternaria Arthrinium Ascospores* Aureobasidium Basidiospores* Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Myrothecium Nigrospora Other brown Other colorless Penicillium/Aspergillus types† Pithomyces Rusts* 1 1 53 13 1 13 1 53 1 13 1 13 3 40 3 160 1 53 Smuts*, Periconia, Myxomycetes* Stachybotrys Stemphylium Torula Trichocladium Ulocladium Zygomycetes Background debris (1-4+)†† 3+ 2+ 2+ Sample volume (liters) 75 75 75 § TOTAL SPORE/m3 130 < 13 2+ 75 27 250 Comments: * Most of these spore types are not seen with culturable methods (Andersen sampling), although some may appear as non-sporulating fungi. Most of the basidiospores are "mushroom" spores while the rusts and smuts are plant pathogens. † The spores of Aspergillus and Penicillium (and others such as Acremonium, Paecilomyces) are small and round with very few distinguishing characteristics. They cannot be differentiated by non-viable sampling methods. Also, some species with very small spores are easily missed, and may be undercounted. ††Background debris indicates the amount of non-biological particulate matter present on the trace (dust in the air) and the resulting visibility for the analyst. It is rated from 1+ (low) to 4+ (high). Counts from areas with 4+ background debris should be regarded as minimal counts and may be higher then reported. It is important to account for samples volumes when evaluating dust levels. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total Spores/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 520993, Page 11 of 11 EMLab P&K Report for: Ms. Karen Shockley Aurora Industrial Hygiene, Inc. 1094 Cudahy Place Suite 120 San Diego, CA 92110 Regarding: Project: UCSD-Literature Building EML ID: 521481 Approved by: Dates of Analysis: Spore trap analysis: 03-16-2009 Lab Manager Magzoub Ismail Project SOPs: Spore trap analysis (I100000) This coversheet is included with your report in order to comply with AIHA and ISO accreditation requirements. For clarity, we report the number of significant digits as calculated; but, due to the nature of this type of biological data, the number of significant digits that is used for interpretation should generally be one or two. All samples were received in acceptable condition unless noted in the Report Comments portion in the body of the report. Due to the nature of the analyses performed, field blank corrections of results is not a standard practice. The results relate only to the items tested. EMLab P&K ("the Company") shall have no liability to the client or the client's customer with respect to decisions or recommendations made, actions taken or courses of conduct implemented by either the client or the client's customer as a result of or based upon the Test Results. In no event shall the Company be liable to the client with respect to the Test Results except for the Company's own willful misconduct or gross negligence nor shall the Company be liable for incidental or consequential damages or lost profits or revenues to the fullest extent such liability may be disclaimed by law, even if the Company has been advised of the possibility of such damages, lost profits or lost revenues. In no event shall the Company's liability with respect to the Test Results exceed the amount paid to the Company by the client therefor. Document Number: 200091 - Revision Number: 5 EMLab P&K 9089 Clairemont Mesa Blvd, Suite 106, San Diego, CA 92123 (866) 465-6653 Fax (858) 569-5806 www.emlab.com Date of Sampling: 03-12-2009 Date of Receipt: 03-12-2009 Date of Report: 03-16-2009 Client: Aurora Industrial Hygiene, Inc. C/O: Ms. Karen Shockley Re: UCSD-Literature Building SPORE TRAP REPORT: NON-VIABLE METHODOLOGY Location: Comments (see below) Lab ID-Version‡: Alternaria Arthrinium Ascospores* Aureobasidium Basidiospores* Bipolaris/Drechslera group Botrytis Chaetomium Cladosporium Curvularia Epicoccum Fusarium Myrothecium Nigrospora Other colorless Penicillium/Aspergillus types† Pithomyces Rusts* 0312-01: Room 242 0312-02: Outdoors, near SW corner None None 2308752-1 2308753-1 raw ct. spores/m3 raw ct. spores/m3 1 13 1 13 2 67 6 320 1 53 Smuts*, Periconia, Myxomycetes* Stachybotrys Stemphylium Torula Ulocladium Zygomycetes Background debris (1-4+)†† 3+ Sample volume (liters) 75 § TOTAL SPORE/m3 1+ 75 350 120 Comments: * Most of these spore types are not seen with culturable methods (Andersen sampling), although some may appear as non-sporulating fungi. Most of the basidiospores are "mushroom" spores while the rusts and smuts are plant pathogens. † The spores of Aspergillus and Penicillium (and others such as Acremonium, Paecilomyces) are small and round with very few distinguishing characteristics. They cannot be differentiated by non-viable sampling methods. Also, some species with very small spores are easily missed, and may be undercounted. ††Background debris indicates the amount of non-biological particulate matter present on the trace (dust in the air) and the resulting visibility for the analyst. It is rated from 1+ (low) to 4+ (high). Counts from areas with 4+ background debris should be regarded as minimal counts and may be higher then reported. It is important to account for samples volumes when evaluating dust levels. The Limit of Detection is the product of a raw count of 1 and 100 divided by the percent read. The analytical sensitivity (counts/m3) is the product of the Limit of Detection and 1000 divided by the sample volume. ‡ A "Version" greater than 1 indicates amended data. § Total Spores/m3 has been rounded to two significant figures to reflect analytical precision. TestAmerica Environmental Microbiology Laboratory, Inc. EMLab ID: 521481, Page 1 of 1