Product datasheet
Anti-Lamin B1 antibody ab90169
4 Images
Overview
Product name
Anti-Lamin B1 antibody
Description
Chicken polyclonal to Lamin B1
Tested applications
ICC/IF, WB
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human Lamin
B1.Read Abcam's proprietary immunogen policy(Peptide available as ab16375.)
Positive control
This antibody gave a positive signal in the following human whole cell lysates: HeLa; Jurkat;
A431; NIH3T3.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
This product may contain up to 3% BSA depending on the batch. For specific batch formulations
please contact us.
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab90169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
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Application
Abreviews
Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa
(predicted molecular weight: 66 kDa).Can be blocked with Mouse Lamin B1
peptide (ab16375).
Target
Function
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the
inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and
may also interact with chromatin.
Involvement in disease
Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adultonset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating
leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early
autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of
the CNS. It differs from multiple sclerosis and other demyelinating disorders in that
neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination
and lack of astrogliosis.
Sequence similarities
Belongs to the intermediate filament family.
Post-translational
modifications
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation.
Increased phosphorylation of the lamins occurs before envelope disintegration and probably
plays a role in regulating lamin associations.
Cellular localization
Nucleus inner membrane.
Anti-Lamin B1 antibody images
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Predicted band size : 66 kDa
Lanes 1, 3 and 5: Wild-type HAP1 nuclear
lysate (20 µg)
Lanes 2, 4 and 6: Lamin B1 knockout
HAP1 nuclear lysate (20 µg)
Lanes 1 and 2: Green signal from target ab90169 observed at 75 kDa
Lanes 3 and 4: Red signal from loading
control - ab10799 observed at 18 kDa
Western blot - Anti-Lamin B1 antibody (ab90169)
Lanes 5 and 6: Merged (red and green)
signal
ab90169 was shown to specifically react with
lamin B1 when lamin B1 knockout samples
were used (in which a mutation has induced
an N-terminal truncation of lamin B1). Wildtype and lamin B1 knockout samples were
subjected to SDS-PAGE. ab90169 and
ab10799 (loading control to histone H3) were
both diluted at 1/1000 and incubated
overnight at 4°C. Blots were developed with
goat anti-rabbit IgG (H + L) and goat antimouse IgG (H + L) secondary antibodies at
1/10 000 dilution for 1 h at room temperature
before imaging.
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All lanes : Anti-Lamin B1 antibody
(ab90169) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma
cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblastlike cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma
cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Western blot - Lamin B1 antibody (ab90169)
Secondary
Goat polyclonal to Chicken IgY - H&L (HRP)
at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 66 kDa
Observed band size : 73 kDa
Additional bands at : 28 kDa,37 kDa,50
kDa. We are unsure as to the identity of these
extra bands.
Exposure time : 4 minutes
ICC/IF image of ab90169 stained HepG2
cells. The cells were 4% PFA fixed (10 min)
and then incubated in 1%BSA / 10% normal
Goat serum / 0.3M glycine in 0.1% PBSTween for 1h to permeabilise the cells and
block non-specific protein-protein
interactions. The cells were then incubated
with the antibody ab90169 at 5ug overnight at
Immunocytochemistry/ Immunofluorescence Lamin B1 antibody (ab90169)
+4°C. The secondary antibody (green) was
Alexa Fluor® 488 Goat anti- chicken IgY
(H+L) used at a 1/1000 dilution for 1h. Alexa
Fluor® 594 WGA was used to label plasma
membranes (red) at a 1/200 dilution for 1h.
DAPI was used to stain the cell nuclei (blue)
at a concentration of 1.43µM.
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Anti-Lamin B1 antibody (ab90169) at 1 µg/ml
+ NIH 3T3 (Mouse embryonic fibroblast cell
line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to
Chicken IgY - H&L (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Western blot - Lamin B1 antibody (ab90169)
Predicted band size : 66 kDa
Observed band size : 75 kDa
Additional bands at : 18 kDa. We are
unsure as to the identity of these extra bands.
Exposure time : 20 minutes
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