Product datasheet
Anti-RGS19 antibody ab72085
1 Abreviews 2 Images
Overview
Product name
Anti-RGS19 antibody
Description
Rabbit polyclonal to RGS19
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
Immunogen
A portion of amino acids 1-50 of human RGS19 (NP_005864).
Positive control
Ramos, mouse placenta lysate and human liver tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab72085 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
IHC-P
Application notes
IHC-P: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 0.5 - 2 µg/ml. Detects bands of approximately 25 and 50 kDa
(predicted molecular weight: 25 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Target
Function
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby
driving them into their inactive GDP-bound form. Binds to G-alpha subfamily 1 members, with the
order G(i)a3 > G(i)a1 > G(o)a >> G(z)a/G(i)a2. Activity on G(z)-alpha is inhibited by
phosphorylation and palmitoylation of the G-protein.
Tissue specificity
Highest expression in lung. Placenta, liver and heart also express high levels of GAIP.
Sequence similarities
Contains 1 RGS domain.
Post-translational
modifications
Fatty acylated. Heavily palmitoylated in the cysteine string motif.
Phosphorylated, mainly on serine residues.
Cellular localization
Membrane.
Anti-RGS19 antibody images
All lanes : Anti-RGS19 antibody (ab72085)
at 1 µg/ml
Lane 1 : Mouse placenta lysate
Lane 2 : Mouse placenta lysate plus
immunizing peptide
Western blot - RGS19 antibody (ab72085)
Predicted band size : 25 kDa
Observed band size : 25 kDa
Additional bands at : 50 kDa. We are
unsure as to the identity of these extra bands.
Immunohistochemical analysis of RGS19 in
paraffin embedded, formalin fixed human liver
tissue using an isotype control (top) and
ab72085 (bottom) at 5µg/ml.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - RGS19 antibody
(ab72085)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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