Product datasheet
Anti-USP28 antibody ab130351
1 Image
Overview
Product name
Anti-USP28 antibody
Description
Goat polyclonal to USP28
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Guinea pig, Cow, Dog, Pig
Immunogen
Synthetic peptide: SVELKHYIQEDN with a Cysteine residue linker, corresponding to internal
sequence amino acids 674-685 of Human USP28 (NP_065937.1).
Run BLAST with
Positive control
Run BLAST with
Human Testis tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Purity
Immunogen affinity purified
Purification notes
Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity
chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab130351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
1
Application
Abreviews
WB
Notes
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
Preliminary experiments gave bands at approx 150kDa and 50kDa in Human
Bone Marrow lysates after 0.2µg/ml antibody staining. Please note that currently
we cannot find an explanation in the literature for the bands we observe given the
calculated size of 122kDa according to NP_065937.1. Both detected bands
were successfully blocked by incubation with the immunizing peptide.
IHC-P
Use a concentration of 2.5 - 3.8 µg/ml. Perform heat mediated antigen retrieval
with citrate buffer pH 6 before commencing with IHC staining protocol.
Target
Function
Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene
stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of
the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by
deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting
complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically
deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the
proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the
nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it
does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC
degradation and explaining the selective MYC degradation in the nucleolus.
Sequence similarities
Belongs to the peptidase C19 family. USP28 subfamily.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Post-translational
modifications
Degradaded upon nickel ion level or hypoxia exposure.
Phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR.
Cellular localization
Nucleus > nucleoplasm.
Anti-USP28 antibody images
ab130351, at 3.8 µg/ml staining USP28 in
paraffin-embedded Human testis tissue by
Immunohistochemistry, with Alkaline
Phosphatase detection.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-USP28
antibody (ab130351)
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