Product datasheet
Anti-FABP4 (phospho Y20) antibody ab85875
3 Images
Overview
Product name
Anti-FABP4 (phospho Y20) antibody
Description
Rabbit polyclonal to FABP4 (phospho Y20)
Tested applications
IHC-P, WB
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Sheep, Rabbit, Cow, Pig
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human
FABP4.Read Abcam's proprietary immunogen policy(Peptide available as ab107889.)
Positive control
This antibody gave a positive signal in the following tissue lysates: Adipose (human); Adipose
(rat); Mouse Brown adipose tissue. IHC-P: FFPE human lung tissue sections.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab85875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-P
Abreviews
Notes
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with
citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa
(predicted molecular weight: 14 kDa).
1
Target
Function
Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers
long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
Cellular localization
Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a
nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive
nuclear export.
Anti-FABP4 (phospho Y20) antibody images
IHC image of FABP4 staining in human
lung formalin fixed paraffin embedded tissue
section, performed on a Leica Bond system
using the standard protocol F. The section
was pre-treated using heat mediated antigen
retrieval with sodium citrate buffer (pH6,
epitope retrieval solution 1) for 20 mins. The
section was then incubated with ab85875,
5µg/ml, for 15 mins at room temperature and
detected using an HRP conjugated compact
Immunohistochemistry (Formalin/PFA-fixed
polymer system. DAB was used as the
paraffin-embedded sections) - Anti-FABP4
chromogen. The section was then
(phospho Y20) antibody (ab85875)
counterstained with haematoxylin and
mounted with DPX.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
2
All lanes : Anti-FABP4 (phospho Y20)
antibody (ab85875) at 1 µg/ml
Lane 1 : Adipose (Human) Total Protein
Lysate - adult normal tissue (ab28980)
Lane 2 : Adult Rat Brown Adipose Tissue
Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Western blot - FABP4 antibody (ab85875)
Goat Anti-Rabbit IgG H&L (HRP)
preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 14 kDa
Observed band size : 14 kDa
Exposure time : 16 minutes
Anti-FABP4 (phospho Y20) antibody
(ab85875) at 1 µg/ml + Adult Mouse Brown
Adipose Tissue Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP)
preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Western blot - Anti-FABP4 antibody (ab85875)
Predicted band size : 14 kDa
Observed band size : 14 kDa
Additional bands at : 35 kDa,49 kDa,85
kDa. We are unsure as to the identity of these
extra bands.
Exposure time : 16 minutes
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
3
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
4