Product datasheet
Anti-Calmodulin antibody ab105498
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Overview
Product name
Anti-Calmodulin antibody
Description
Rabbit polyclonal to Calmodulin
Tested applications
WB
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Chicken, Cow, Zebrafish, Orangutan
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of
Human Calmodulin.Read Abcam's proprietary immunogen policy
Positive control
This antibody gave a positive signal in the following whole cell lysates: Ramos; NIH3T3.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a
stabilising agent. If you would like information about the formulation of a specific lot, please
contact our scientific support team who will be happy to help.
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab105498 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 16 kDa
(predicted molecular weight: 16 kDa).
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Target
Function
Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+).
Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein
kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway
that regulates the centrosome cycle and progression through cytokinesis.
Sequence similarities
Belongs to the calmodulin family.
Contains 4 EF-hand domains.
Post-translational
modifications
Ubiquitination results in a strongly decreased activity.
Phosphorylation results in a decreased activity.
Cellular localization
Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed
throughout the cell during interphase, but during mitosis becomes dramatically localized to the
spindle poles and the spindle microtubules.
Form
There are three genes which encode an identical calcium binding protein which is one of the four
subunits of phosphorylase kinase.
Anti-Calmodulin antibody images
All lanes : Anti-Calmodulin antibody
(ab105498) at 1 µg/ml
Lane 1 : Ramos (Human Burkitt's lymphoma
cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic
fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Western blot - Anti-Calmodulin antibody
Goat Anti-Rabbit IgG H&L (HRP)
(ab105498)
preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 16 kDa
Observed band size : 16 kDa
Exposure time : 20 minutes
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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