Product datasheet
Myeloperoxidase (MPO) Inhibitor Screening Assay Kit
ab133080
2 Images
Overview
Product name
Myeloperoxidase (MPO) Inhibitor Screening Assay Kit
Precision
Intra-assay
Sample
n
Mean
SD
CV%
Chlorination
16
3.7%
Peroxidation
16
2.8%
Chlorination
16
3.7%
Peroxidation
16
2.8%
Inter-assay
Sample
n
Mean
SD
CV%
Chlorination
16
3.6%
Peroxidation
16
3.1%
Tests
2 x 96 well plate
Assay type
Quantitative
Species reactivity
Reacts with: Human
Predicted to work with: all Mammals
Product overview
Abcam’s Myeloperoxidase (MPO) Inhibitor Screening Assay (ab133080) provides convenient
fluorescence-based methods for screening inhibitors to both the chlorination and peroxidation
activities of MPO. The chlorination assay utilizes the non-fluorescent 2-[6-(4-aminophenoxy)-3oxo-3H-xanthen-9-yl]-benzoic acid (APF), which is selectively cleaved by hypochlorite (-OCl) to
yield the highly fluorescent compound fluorescein. Fluorescein fluorescence is analyzed with an
excitation wavelength of 480-490 nm and an emission wavelength of 515-520 nm. The
peroxidation assay utilizes the peroxidase component of MPO, where a single two electron
oxidation of native enzyme (MPO) to compound I (MPO-I) is followed by two successive one
electron reductions back to native enzyme by compound II (MPO-II). The reaction between
hydrogen peroxide and ADHP (10-acetyl-3,7-dihydroxyphenoxazine) produces the highly
fluorescent compound resorufin. Resorufin fluorescence is analyzed with an excitation
wavelength of 530-540 nm and an emission wavelength of 585-595 nm.
Tested applications
Functional Studies
1
Properties
Storage instructions
Please refer to protocols.
Components
2 x 96 tests
96-Well Plate (black)
2 units
96-Well Plate Cover
2 units
MPO Assay Buffer
1 vial
MPO Chlorination Substrate
1 vial
MPO DMSO
1 vial
MPO Hydrogen Peroxide
1 vial
MPO Inhibitor
1 vial
MPO Peroxidation Substrate
2 vials
Myeloperoxidase Control
1 vial
Function
Part of the host defense system of polymorphonuclear leukocytes. It is responsible for
microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes
the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and
other toxic intermediates that greatly enhance PMN microbicidal activity.
Involvement in disease
Defects in MPO are the cause of myeloperoxidase deficiency (MPD) [MIM:254600]. MPD is an
autosomal recessive defect that results in disseminated candidiasis.
Sequence similarities
Belongs to the peroxidase family. XPO subfamily.
Cellular localization
Lysosome.
Applications
Our Abpromise guarantee covers the use of ab133080 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Functional Studies
Abreviews
Notes
Use at an assay dependent concentration.
Myeloperoxidase (MPO) Inhibitor Screening Assay Kit images
2
Inhibition of MPO chlorination activity by 4aminobenzhydrazide (IC50 = 5 nM).
Functional Studies - Myeloperoxidase (MPO)
Inhibitor Screening Assay Kit (ab133080)
Inhibition of MPO peroxidation activity by 4aminobenzhydrazide (IC50 = 0.4 µM).
Functional Studies - Myeloperoxidase (MPO)
Inhibitor Screening Assay Kit (ab133080)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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