Product datasheet
Anti-Glutathione Peroxidase 1 antibody ab50427
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Overview
Product name
Anti-Glutathione Peroxidase 1 antibody
Description
Goat polyclonal to Glutathione Peroxidase 1
Specificity
This antibody is expected to recognise isoform 1 ( NP_000572.2) only.
Tested applications
IHC-Fr, IHC-P, WB, ELISA
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Guinea pig, Chimpanzee, Macaque Monkey, Marmoset (common)
Immunogen
Synthetic peptide:
C-REALPAPSDDATA
, corresponding to internal sequence amino acids 128-140 of Human Glutathione Peroxidase 1
Run BLAST with
Positive control
Run BLAST with
Human Liver and kidney lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab50427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-Fr
Use a concentration of 20 µg/ml.
IHC-P
Use a concentration of 3 - 5 µg/ml.
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Application
Abreviews
Notes
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 22 kDa
(predicted molecular weight: 22 kDa).
ELISA
Use at an assay dependent concentration. Antibody detection limit dilution
1:32,000.
Target
Function
Protects the hemoglobin in erythrocytes from oxidative breakdown.
Sequence similarities
Belongs to the glutathione peroxidase family.
Cellular localization
Cytoplasm.
Anti-Glutathione Peroxidase 1 antibody images
ab50427 (20µg/ml) staining of PFA-perfused
cryosection of Porcin Kidney following
microwave antigen retrieval with citrate buffer
pH 3 and CY3-staining shows staining of
cytoplasm in PCT epithelial cells.
Immunohistochemistry (Frozen sections) - AntiGlutathione Peroxidase 1 antibody (ab50427)
Anti-Glutathione Peroxidase 1 antibody
(ab50427) at 1 µg/ml + Human Liver lysate
(35µg protein in RIPA buffer).
Predicted band size : 22 kDa
Observed band size : 22 kDa
Primary incubation was 1 hour. Detected by
chemiluminescence.
Western blot - Glutathione Peroxidase 1 antibody
(ab50427)
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ab50437 at 3µg/ml staining Glutathione
Perxidase 1 in human cerebral cortex by
Immunohistochemistry (Formalin/ PFA-fixed
paraffin-embedded tissue sections). The
tissue underwent antigen retrieval by steam in
Immunohistochemistry (Formalin/PFA-fixed
citrate buffer pH 6.0. The AP staining
paraffin-embedded sections) - Glutathione
procedure was used for staining.
Peroxidase 1 antibody (ab50427)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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