Product datasheet
Anti-Glucagon antibody ab36232
1 Image
Overview
Product name
Anti-Glucagon antibody
Description
Sheep polyclonal to Glucagon
Specificity
ab36232 recognises the C terminal region of glucagon.
Tested applications
RIA, IHC-Fr, ICC/IF, IHC-P
Species reactivity
Reacts with: Mouse, Rat, Human, Pig
Immunogen
Zinc glucagon conjugated to BSA
Positive control
Stains cells containing glucagon in human and mouse pancreas.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS, pH 7.2
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab36232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
RIA
Use at an assay dependent dilution.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent dilution.
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Target
Relevance
Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes
insulin by converting glycogen to glucose in the liver and increasing blood sugar levels.
Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal
peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons
hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and
drinking behavior. GLP2 stimulates intestinal epithelial growth.
Cellular localization
Secreted
Anti-Glucagon antibody images
Immunohistochemical examination of
monoamine oxidase type B (MAOB)
localization in A cells and B cells of the rat
pancreatic islet. Sections were processed for
a double-labeling immunofluorescence
method in combination of anti-MAOB (Cy3)
with either anti-glucagon ab36232
(fluorescein) or anti-insulin (fluorescein), and
then observed with a confocal laser scanning
microscope. (a,b) MAOB staining (red). (c)
Glucagon staining (green). (d) Insulin staining
Immunocytochemistry/ Immunofluorescence -
(green). (e) Superimposition of the images in
Anti-Glucagon antibody (ab36232)
a and c. (f) Superimposition of the images in
b and d. Note that all of A cells that contain
glucagon are also stained for MAOB (a,c,e),
and all of B cells are also stained for MAOB
(b,d,f). Original magnification x 100. Bar = 10
µm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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