Product datasheet
Anti-Sodium Iodide Symporter antibody [SPM186]
ab17795
2 Abreviews 3 References 5 Images
Overview
Product name
Anti-Sodium Iodide Symporter antibody [SPM186]
Description
Mouse monoclonal [SPM186] to Sodium Iodide Symporter
Specificity
ab17795 shows strong immunostaining in Graves disease tissues, intermediate staining in
papillary and follicular thyroid cancer, and no staining in Hurtle cell cancer or in nonthyroidal
tissue.
Tested applications
WB, IHC-P, ICC/IF, Flow Cyt
Species reactivity
Reacts with: Rat, Human
Immunogen
Synthetic peptide:
WTPCV GHDGGRDQQE TNL
, corresponding to amino acids 625-643 of Human Sodium Iodide Symporter.
Run BLAST with
Positive control
Run BLAST with
Thyroid lysate, thyroid tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: BSA, 10mM PBS, pH 7.4
Purity
Protein G purified
Clonality
Monoclonal
Clone number
SPM186
Isotype
IgG1
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab17795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
WB
Notes
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 75 kDa
(predicted molecular weight: 69 kDa).
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC
staining protocol. Boil tissue sections in 10 mM citrate buffer, pH 6.0 for 10
minutes followed by cooling at room temperature for 20 minutes.
ICC/IF
Use at an assay dependent concentration. PubMed: 23342072
Flow Cyt
Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as
an isotype control with this antibody.
Target
Function
Mediates iodide uptake in the thyroid gland.
Tissue specificity
Expression is primarily in thyroid tissue, but also to a lower extent in mammary gland and ovary.
Expression is reduced in tumors.
Involvement in disease
Thyroid dyshormonogenesis 1
Sequence similarities
Belongs to the sodium:solute symporter (SSF) (TC 2.A.21) family.
Cellular localization
Membrane.
Anti-Sodium Iodide Symporter antibody [SPM186] images
ab17795 staining Sodium Iodide Symporter
in Rat thyrocytes (Pccl3) by ICC/IF
(Immunocytochemistry/immunofluorescence).
Cells were fixed with 4% paraformaldehyde,
permeabilized with 0.1% saponin and
blocked with 5% BSA for 1 hour at 37°C.
Samples were incubated with primary
antibody (1/50 in PBS + 1% BSA + 0.1%
saponin) for 16 hours at 4°C. An Alexa Fluor®
Immunocytochemistry/ Immunofluorescence -
488-conjugated Goat anti-mouse IgG
Anti-Sodium Iodide Symporter [SPM186] antibody
polyclonal (1/300) was used as the secondary
(ab17795)
antibody.
This image is courtesy of an Abreview submitted by
Virginie Joris
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ab17795 staining Sodium Iodide
Symporter in Human thyrocytes (primary
culture) by ICC/IF
(Immunocytochemistry/immunofluorescence).
Cells were fixed with 4% paraformaldehyde,
permeabilized with 0.1% saponin in PBS and
blocked with 5% BSA + 0.1% saponin in PBS
for 1 hour at 37°C. Samples were incubated
with primary antibody (1/50 in PBS + 1% BSA
Immunocytochemistry/ Immunofluorescence -
+ 0.1% saponin) for 16 hours at 4°C. An
Anti-Sodium Iodide Symporter antibody [SPM186]
Alexa Fluor® 488-conjugated Goat anti-
(ab17795)
mouse IgG polyclonal (1/300) was used as
This image is courtesy of an anonymous Abreview
the secondary antibody.
ab17795 at 1/50 staining sodium iodide
symporter from human thyroid by
immunohistochemistry (formalin-fixed, paraffin
embedded).
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Sodium Iodide
Symporter antibody [SPM186] (ab17795)
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Anti-Sodium Iodide Symporter antibody
[SPM186] (ab17795) at 1 µg/ml + Breast
(Human) Tissue Lysate - normal tissue
(ab30090) at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP)
preadsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Western blot - Sodium Iodide Symporter antibody
[SPM186] (ab17795)
Predicted band size : 69 kDa
Observed band size : 75 kDa
Additional bands at : 46 kDa. We are
unsure as to the identity of these extra bands.
Exposure time : 12 minutes Sodium Iodide
Symporter contains a number of potential
glycosylation sites (SwissProt) which may
explain its migration at a higher molecular
weight than predicted.
Overlay histogram showing MCF7 cells
stained with ab17795 (red line). The cells
were fixed with 4% paraformaldehyde (10
min) and then permeabilized with 0.1% PBSTween for 20 min. The cells were then
incubated in 1x PBS / 10% normal goat
serum / 0.3M glycine to block non-specific
Flow Cytometry - Sodium Iodide Symporter
antibody [SPM186] (ab17795)
protein-protein interactions followed by the
antibody (ab17795, 1µg/1x106 cells) for 30
min at 22ºC. The secondary antibody used
was DyLight® 488 goat anti-mouse IgG (H+L)
(ab96879) at 1/500 dilution for 30 min at
22ºC. Isotype control antibody (black line) was
mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106
cells) used under the same conditions.
Acquisition of >5,000 events was performed.
This antibody gave a positive signal in MCF7
cells fixed with 80% methanol (5
min)/permeabilized in 0.1% PBS-Tween used
under the same conditions.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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