Proceedings from the Third Workshop on Genetics of Bark Beetles and Associated Microorganisms In Association with IUFRO WP 7.03.05 - Integrated Control of Scolytid Bark Beetles Workshop Organizers: Barbara Bentz, Anthony Cognato, and Kenneth Raffa Contents Phylogenetics of Bark Beetles 1 Reconstructing the Phylogeny of Scolytinae and Close Allies: Major Obstacles and Prospects for a Solution by Bjarte H. Jordal. ........................................................................................................3 A Standard DNA Taxonomy for Insects? by Anthony I. Cognato.......................................................11 Population Genetics of Bark Beetles 13 Pitfalls in Applying Mitochondrial Markers Onto the Scolytid Species Pityogenes chalcographus by Wolfgang Arthofer, Dimitrios N. Avtzis, Markus Riegler,, Wolfgang J. Miller, and ���������� Christian �������� Stauffer.........15 Invasion Genetics of Emerald Ash Borer (Agrilus planipennis FAIRMAIRE) in North America by Alicia M. Bray, Leah S. Bauer, Robert A. Haack, Therese Poland, and James J. Smith. ............................21 Genetic and Phenotypic Resistance in Lodgepole Pine to Attack by Mountain Pine Beetle by Alvin Yanchuk and Kimberly Wallin......................................................................................................23 Genetic Architecture of Differences in Fitness Traits Among Geographically Separated Dendroctonus ponderosae Populations by Barbara Bentz, Mike Pfrender, Ryan Bracewell, andKaren Mock.................25 Phylogeography of Dendroctonus rufipennis Based on mtDNA and Microsatellites by Kenneth F. Raffa, Luana S. Maroja, Steven M. Bogdanowicz, Kimberly F. Wallin, and Richard G. Harrison.......................27 Phylogeographic Analysis of the Douglas-fir Beetle Dendroctonus pseudotsugae Hopkins (Coleoptera: Curculionidae: Scolytinae) by Enrico A. Ruíz, Jane L. Hayes, John E. Rinehart, and G. Zúñiga..........29 Evolutionary Ecology of Pheromone Signaling in Dendroctonus frontalis by Deepa S. Pureswaran, Brian T. Sullivan, and Matthew P. Ayres.................................................................................................33 Bark Beetle Gene Structure and Function 35 Red Turpentine Dendroctonus valens P450s: Diversity, Midgut Location, and Response to Alfapinene Enantiomers by M. Fernanda López, Viviana Cerrillo, Zulema Gómez, M. Shibayama, Olga-Arciniega, and Gerardo Zúñiga............................................................................................37 Expression Patterns of Bark Beetle Cytochromes P450 During Host Colonization: Likely Physiological Functions and Potential Targets for Pest Management by Dezene P. W. Huber, Melissa Erickson, Christian Leutenegger, Joerg Bohlmann, and Steven J. Seybold. ....................................41 Genetics of Symbionts, Natural Enemies, and Hosts 43 Yeasts Associated with Bark Beetles of the Genus Dendroctonus erichson (Coleoptera: Curculionidae: Scolytinae): Molecular Identification and Biochemical Characterization by Flor N. Rivera, Zulema Gómez, Evelyn González, Nydia López, César H. Hernández Rodríguez, and Gerardo Zúñiga..................45 Gut Bacteria of Bark and Wood Boring Beetles by Archana Vasanthakumar, Yasmin Cardoza, Italo Delalibera, Patrick Schloss, Jo Handelsman, Kier Klepzig, and Kenneth Raffa.......................................49 i