This file was created by scanning the printed publication. Errors identified by the software have been corrected; however, some errors may remain. Preface Species of the genus Populus (poplars and aspens) are interspersed primarily in temperate and cold-region forests of the northern hemisphere. They are relatively shortlived, fast-growing trees that can grow on marginal soils and are widely adaptable. Populus systems are rapidly being developed for application in fiber, fuel, and environmental plantings. In addition, contributions of in vitro and molecular biological studies of Populus are apparent in virtually every area of forest biology and ecology. The future of Populus research appears bright and promises significant biotechnological advances toward our understanding of processes and interactions in forest ecosystems. The concepts for this book were generated from a survey that identified the need to synthesize diverse, yet related, information from the rapidly developing studies on Populus molecular biology and in vitro culture. The topics included were designed to complement the recently published book, Biology of Populus and its implications for management and conseroation (Stettler, R.F.; Bradshaw, H. D., Jr.; Heilman, P.E.; Hinckley, T.M., eds. 1996. Ottawa, Ontario, Canada: NRC Research Press. 539 p.). Although we attempted to direct chapters toward a "more general" audience, not all topics were amenable. Lead authors were invited and encouraged to collaborate with co-authors working in similar areas, and we were especially supportive of collaboration among diverse, international laboratories. We thank all authors for their stellar efforts and valuable contributions. We also appreciate the constructive advice from peer reviewers, who are listed in the appendix, on all chapters. 1his book was designed to foster information exchange and to encourage research cooperation. U.S. Department of Agriculture (USDA) funding was obtained to distribute this publication at a minimal cost and to broaden information access. Unfortunately, hampered by USDA Forest Service budget cuts and downsizing, associated publication delays ensued. These delays were minimized by the efforts and iv perseverance of the Rocky Mountain Forest and Range Experiment Station publication staff. We appreciate their patience during the extended publication process. Administrative support is essential for the success of all projects, especially those of substantial proportion. USDA Forest Service administrative support for this significant endeavor was provided by Dr. Barbara C. Weber (Associate Deputy Chief for Research, USDA Forest Service), Dr. Calvin F. Bey (Director, Forest Management Research, USDA Forest Service, retired), Dr. J. Robert Bridges (Staff Research Entomologist, Forest Insect and Disease Research), Dr. Denver P. Burns (Director, Rocky Mountain Forest and Range Experiment Station [RMFRES]), Dr. Brian M. Kent (Assistant Director for Research, RMFRES), Dr. Michele M. Schoeneberger (Project Leader, RM-4551, RMFRES), and Mr. Lane G. Eskew (Supervisory Technical Publications Editor, RMFRES). We appreciate the administrative support from these people that helped this project proceed during difficult times. The diligent perseverance of several people provided the driving force behind this publication and was instrumental in improving overall quality. The proficient efforts of Richard C. Carman were especially outstanding. Richard maintained general organization in the multi-step process, conducted correspondence, performed preliminary technical editing, prepared the index, and sustained the voluminous records. Special recognition is extended to Madelyn C. Dillon, who conducted the primary technical editing and ensured continued progress with her devoted vigilance and persistence. Karen Mora helped to produce this publication's high-quality visual information and professional layout. Joyce Patterson designed and created the book's aesthetically pleasing cover art. Rudy M. King provided the biometrics review of all chapters. Loretta J. Ulibarri with the assistance of Derald L. Dunagan, and Sharon M. Powers (U.S. Geological Survey, Water Resources Division) completed the indexing process, a task of monumental proportions. We are grateful to all these people for their valuable contributions. We especially thank our friends and colleagues for their overall assistance, and hope that this publication will serve as a basis for future research with Populus and other tree genera. Ned B. Klopfenstein Young Woo Chun Mee-Sook Kim M. Raj Ahuja USDA Forest Service Gen. Tech. Rep. AM·GTA·297. 1997.