Poster Presentation
Solvent Free HPLC Analysis of Basic Drugs
Tariq Ahmad
Faculty Mentor: Tarab Ahmad
Chemistry
High pressure liquid chromatography (HPLC) is the most versatile technique for quickly analyzing large numbers of different samples: checking purity in the development of drugs and quality assurance in the process of quality control of final products. Also preparative HPLC or liquid chromatography (LC) is used to purify pharmaceuticals from impurities. The amounts obtained from each chromatographic purification range from grams to kilograms. This process that use LC consumes large amounts of volatile organic solvents like methanol, acetontirile and hexane.
The introduction of cleaner technologies has become a major concern throughout both industry and academia. Therefore, the search for alternatives to the most damaging solvents has become a high priority.
Most organic salts are solid at ambient temperature, but ionic liquids (IL) or room temperature ionic liquids (RTIL) are a type of salts that are liquid at low temperature. Because RTIL are good solvents for both inorganic and organic materials, they are non-volatile, nonflammable, thermally stable, and recyclable solvents and they have some particular properties in chemical reactions and they have the potential to replace the organic solvents in the HPLC analysis. We will investigate in this research alternative solvents that could be used for the reversedphase HPLC analysis of drugs like nortryptline and amitryptyline . We will use aqueous solutions of RTIL like 1methyl,3 butyl immidazolium (BMIM) salts as mobile phase for the HPLC separations of these drugs.