Quantum-dot Linked Immuno-diagnostic Assay for Detection of Cardiac Biomarkers
Michael Boyce-Jacino**, Michael Neidrauer*, Michael Kochersperger**, Chengjie Yu*, Nikolay Voznyak*, Elisabeth S. Papazoglou*
Medical/Market Need
Performance of current detection system
Acute Myocardial Infarction (AMI, heart attack)
Model system: silica microspheres + qdots
(no sandwich immunochemistry)
• Cardiac muscle damage biomarkers (troponin etc) increase over 4-6
hours after event and peak within 24-48 hours (depending on marker)
• 6 million Emergency Dep. visits per year for chest pain
•  Second most common reason to visit ED
• Equivical ECG/Troponin (biomarker) results on admission most common
•  90 minutes to first test, up to 9 hours to clinical decision
• About ½ patients admitted to ICU for AMI don’t need to be
•  #1 cause of medical malpractice lawsuits Immunoassay Performance
Full system (sandwich immunochemistry)
Primary
antibody
QDbiotin
Streptavidin
QD-conjugated
secondary antibody
Antigen
(troponin I)
Silica
microsphere
Silica
microsphere
Silica
microsphere
Silica
microsphere
Silica
microsphere
Early Detection of AMI is Essential to Survival
CURRENT MARKET
TARGET MARKET
Blood Level of Troponin
FUTURE MARKET
Defensive
medicine
0 ng/ml
(blank)
Diagnostic
Predictive
0.005 ng/ml
0.01 ng/ml
0.025 ng/ml
Time
Need: faster and more sensitive assay
Next Steps
Project Evolution
Current
(Start-up Seed Funding)
University
(Coulter funding)
Sample
Primary antibody
Optimize immunochemistry
Envisioned
Product
QD-­‐conjugated secondary antibody
Antigen (troponin I)
Maximize sensitivity Minimize variance
Minimize incubation time
•
•
•
Convert sample holder – Capillary tube to Lateral flow
Silica microsphere
Interact w/ antigen
Silica microsphere
Silica microsphere
Sandwich w/ QD
conjugated Ab*
Op.cal detec.on
window
Sample loading
•
•
Whole blood
Single step procedure
Miniaturize reader à
Sensovation AG
Acknowledgements
Technology development funded by:
Breadboard concept:
Low cost camera, robust
Benchtop prototype:
Simple, flexible design
Handheld concept:
Exploit built-in power of smart
phone with simple, miniaturized
reader concept
Coulter-Drexel Translational Research Partnership Program
•
•
•
•
W. H. Coulter Foundation Ben Franklin Technology Partners
NetScientific Group
* Drexel University School of Biomedical Engineering, Philadelphia, Pennsylvania **QLIDADiagnostics, LLC
Office of
International
Programs
Microspheres in
capillary tube