United States Department of Agriculture Forest Service Rocky Mountain Forest and Range Experiment Station Water Chemistry of Rocky Mountain Front Range Aquatic Ecosystems Fort Collins, Colorado 80526 Research Paper RM-RP-325 Robert C. Musselman Laura Hudnell Mark W. Williams Richard A. Sommerfeld Musselman, Robert C.; Hudnell, Laura; Williams, Mark W.; Sommerfeld, Richard A. Water chemistry of Rocky Mountain front range aquatic ecosystems. Res. Pap. RM-RP-325. Fort Collins, CO: U.S. Department of Agriculture, Forest Service, Rocky Mountain Forest and Range Experiment Station. 13 p. Abstract: A study of the water chemistry of Colorado Rocky Mountain Front Range alpine/subalpine lakes and streams in wilderness ecosystems was conducted during the summer of 1995 by the USDA Forest Service Arapaho and Roosevelt National Forests and Rocky Mountain Forest and Range Experiment Station, and the University of Colorado Institute of Alpine and Arctic Research. Data were collected to examine the water chemistry of Front Range high-elevation lakes and their sensitivity to atmospheric deposition, particularly nitrogen saturation. Water chemistry data from synoptic surveys of high-elevation lakes in wilderness areas of other National Forests in Colorado are also included in this report. Because of the extent, uniqueness, and potential value of the data collected, the entire water chemistry data base including over 265 samples from more than 130 lakes and streams is presented. Preliminary data examination indicates that many lakes have detectible nitrate concentrations, nitrate concentrations are higher early in the season and decrease as the season progresses, and inlets often have higher nitrate concentrations than outlets. Detailed data analysis and interpretation, its relationship to landscape characteristics, and the implications for ecosystem response and management will be presented by the authors in subsequent manuscripts in preparation. Keywords: water chemistry, alpine/subalpine lakes and streams, atmospheric deposition, nitrogen saturation, ecosystems, aquatic Cover photo: Upper Lake in Indian Peaks Wilderness USDA Forest Service Research Paper RM-RP-325 September 1996 Water Chemistry of Rocky Mountain Front Range Aquatic Ecosystems Robert C. Musselman Rocky Mountain Forest and Range Experiment Station1 Laura Hudnell Arapaho and Roosevelt National Forests1 Mark W. Williams Institute of Arctic and Alpine Research2 Richard A. Sommerfeld Rocky Mountain Forest and Range Experiment Station1 1 Headquarters is in Fort Collins, Colorado, in cooperation with Colorado State University 2 University of Colorado at Boulder Contents Page Introduction ................................................................................................... 1 Objectives ...................................................................................................... 1 Western United States Chemistry .............................................................. 2 Nitrogen Deposition ..................................................................................... 2 Nitrogen Saturation ...................................................................................... 3 Methods ......................................................................................................... 3 Sampling Protocols ................................................................................. 4 Analyses .................................................................................................... 4 Results and Discussion ............................................................................... 5 Literature Cited ............................................................................................. 6 iii Water Chemistry of Rocky Mountain Front Range Aquatic Ecosystems Robert C. Musselman, Laura Hudnell, Mark W. Williams, Richard A. Sommerfeld may enter storage pools and runoff may result from the outflow of water from these pools (Kendall et al. 1995). This study concentrated on the Front Range of Colorado. Weber (1976) defines Colorado’s Front Range as the range of mountains visible when approaching Denver from the east; specifically, from Pike’s Peak north along the Continental Divide through Rocky Mountain National Park. Others designate the Front Range as the region from the Colorado and Wyoming border south to the Arkansas River (Arno and Hammerly 1984). Although this study included samples from lakes as far south as the Sangre de Christo Range and north into the southeastern corner of Wyoming, the area of study in this report is referenced as the Front Range. INTRODUCTION Aquatic ecosystems are more susceptible and respond more rapidly to direct input of atmospheric deposition than terrestrial ecosystems (Irving 1991). Atmospheric deposition in the Eastern United States and its affect on aquatic ecosystems are well documented (Irving 1991); however, little is known about the extent or effects of atmospheric deposition on lakes and streams in the Western United States. Lakes and streams that are most susceptible to acidic deposition have low concentrations of anions and cations. This results in low acid neutralizing capacity (ANC) and reduced ability to buffer acidic input. Low ANC lakes are often associated with catchments that have little or poorly developed soils, which reduces soil chemical alteration of precipitation or snowmelt before it enters lakes and streams. Lakes and streams with low ANC and catchments with little soil development are often located at high-elevation recently glaciated sites that have a large percentage of exposed bedrock. Low ANC also indicates that reduced amounts of nutrients are available for aquatic biomass production. Nitrate and phosphate are the nutrients that most frequently limit productivity; therefore, aquatic ecosystems within such catchments often have small biomass amounts. Snowmelt dominates the hydrologic cycle early in the melt season. Large concentrations of nitrates are eluted from the snowpack with the initial melt; some of this enters surface waters directly. However, unless considerable amounts of bedrock are exposed, much of the snowmelt enters talus or soils where biological and chemical alteration rapidly occurs (Campbell et al. 1995). Snowmelt OBJECTIVES This study was a synoptic survey of lake water chemistry, especially nitrate, in the mountainous areas east of the Colorado Continental Divide and in southeastern Wyoming that are exposed to increasing atmospheric emissions. A goal was to include as many high-elevation lakes as possible within a specific sampling time window. For a subsample of selected catchments in the Arapaho and Roosevelt National Forests, the study examined differences between lake inlet and outlet streams, and early season and late season sampling dates. Analyses of relationships between lake chemistry and landscape characteristics, such as soil type, geology, vegetation, terrain, watershed size, lake surface area, and elevation, are not included in this report. 1 tions throughout the season is necessary to determine the interaction between phosphate, nitrate, and biota, and then decide whether nitrate is exported because low phosphate levels limit productivity or because input rates are so high that complete use is impossible. Excess nitrate in aquatic systems also occurs from decomposition when populations decline after phytoplankton blooms, a natural phenomena occurring during cyclic growth patterns in aquatic systems. Excess nitrate can also occur in surface waters from a sudden lake turnover after strong wind or heavy precipitation. Although one source of excess nitrogen is deposition input, soil and biological processes also contribute to excess nitrogen in surface waters. Nitrogen is available from soils but most highelevation sites along Colorado’s Front Range have relatively young, minimally developed soils; nitrate availability from these soils is low. Nitrates in soils are not tightly bound on ion exchange sites. Nitrogen input in high-elevation ecosystems with limited soil development is primarily from atmospheric wet deposition, which is from high amounts of precipitation. In addition, large amounts of nitrogen are temporarily stored in the winter snowpack (Bowman 1992) from wet and dry deposition and become available for biotic use with snowmelt. Although nitrogen input from stream water and snowmelt is an important source of nitrate for biomass productivity in high-elevation lakes, there are alternative sources of nitrate in surface waters. Monitoring aquatic biomass could explain alternative sources of nitrate in Rocky Mountain aquatic ecosystems. This information is unavailable from monitoring water chemistry only. Certain bluegreen algae, such as Anabaena spp and Nostoc spp, fix nitrogen and add nitrate to the system. Algal blooms also influence nitrate levels. During these periods of rapid growth, nitrate concentrations approach zero as all available nitrate is used. However, when these populations die, large amounts of nitrate are released into the water independent of nitrate input from streamflow or snowmelt. Stratification or mixing of lakes also influences the quantity of nitrates available for phytoplankton growth. This occurs as nitrates at deeper levels become unavailable to surface algal populations with stratification or become available with mixing. Mixing of a stratified lake depends WESTERN UNITED STATES LAKE CHEMISTRY Few data are available concerning the current susceptibility to acidification or nitrogen saturation of lakes in the Western United States. Only one major survey of lake susceptibility to acidification in the Western United States has been conducted (Eilers et al. 1987, Landers et al. 1987). This onetime sampling identified lakes, generally at high elevations with poorly developed soils, that might be susceptible to increases in atmospheric deposition because of their low buffering capacity. Among the hundreds of lakes sampled in the 1985 survey (Eilers et al. 1987, Landers et al. 1987) only about 130 were in Colorado and less than a third of these were from wilderness areas sampled in the current study. The Front Range urban corridor of Colorado is experiencing rapid growth and increased nitrogen and sulfur oxide emissions that contribute to nitrate and sulfate atmospheric deposition in the adjacent mountainous areas. In addition, coal fired power plants in Western Colorado emit nitrates and sulfates that contribute to deposition in Front Range ecosystems. NITROGEN DEPOSITION Productivity is limited by the amount of nitrate available for growth in many high-elevation aquatic ecosystems in the Western United States (Williams et al. 1996). Nitrate available for growth and productivity depends upon nitrate input from deposition, weathering, catchment storage, or biomass decomposition. Even small changes in the nitrate levels of aquatic ecosystems can result in large alterations in species assemblages and phytoplankton abundance. If phytoplankton do not use all available nitrate to increase productivity, nitrates are exported and the system is considered saturated (Williams et al. 1996). However, some lakes may be phosphate limited due to a low natural supply of phosphate. Nitrogen export in these lakes may indicate a lack of phosphate rather than an excess of nitrogen (Morris and Lewis 1988). Nitrogen export may represent the seasonal nature of the ionic pulse of nitrate or the inability of the biomass to use nitrate when it is rapidly flushed through the system. Frequent monitoring of water chemistry and phytoplankton popula2 modifications in above-ground and below-ground biodiversity as the growth and survival of certain species are favored over others. Terrestrial ecosystems with sufficient nitrate input can be limited by other nutrients, such as phosphorus, resulting in nitrate export. Soils most at risk to increased nitrogen are those with low: 1) cation exchange capacity, 2) base saturation, 3) clay or organic matter content, 4) sulfate and nitrate adsorption capacity, and those with a pH range of 5.5 to 6.5 (Smith 1992). These are characteristics typical of high-elevation soils. Excess nitrogen input from deposition or from weathering may be historically common at specific seasonal time periods. However, if the nitrogen input is higher than historic levels, particularly during periods of peak seasonal nitrogen use by biota, ecosystem processes may be altered by the additional nitrogen. Recent research indicates that some Colorado Front Range aquatic ecosystems are near or above the saturation point for nitrogen deposition (Williams et al. 1993, 1996). High-elevation alpine aquatic ecosystems are the most susceptible to excess nitrogen (Baron et al. 1994). Small catchments with young, shallow soils, large amounts of exposed, slowly weatherable, quartz or quartzite bedrock, and steep terrain are particularly susceptible to saturation. on basin characteristics and lake size and depth, and can be caused by sudden, local meteorological changes. Temporal variation exists in the concentration of lake nutrients as import and export of nutrients change. Snowmelt flowpaths and processes, groundwater storage and retention, weathering, and soil processes are involved in nitrate concentration changes in surface waters. Interpretation of water chemistry data must consider naturally occurring biotic, weathering, or temporal changes in the nitrate content of lakes and in other possible sources of nitrogen in surface waters. Excess nitrate concentrations can also occur without excess input in Front Range aquatic systems limited by phosphorus (Morris and Lewis 1988). Excess nitrogen can have subtle affects before exportation including increase in productivity or changes in terrestrial and aquatic species composition. Such changes in ecosystem structure and function or in ecosystem productivity may be undesirable if incompatible with management objectives. These subtle changes are evident only with long-term, relatively expensive monitoring of species abundance and biomass in addition to monitoring water chemistry. NITROGEN SATURATION Nitrogen saturation is defined for this report as the export of nitrogen from the system during times of the year when ecosystem biota should be taking up all available nitrogen for growth. Export indicates that more nitrogen is present in the system than can be used by the biota; excess nitrogen is detected in surface waters. This generally occurs during August and early September (Musselman 1994, Williams 1994) after most snowmelt has ceased and temperatures are optimum for production. Nitrogen saturation, as commonly defined, suggests the existence of maximum possible biota use, which indicates that growth is not limited by nitrogen. However, as discussed, other factors can cause export before the point of maximum productivity is reached. Nitrogen saturation can have major impacts on terrestrial ecosystems such as changes in productivity and species composition and increased winter damage (Aber et al. 1989). There can be METHODS High-elevation lakes and first order streams in catchments with a high percentage of exposed bedrock or glaciated landscape were selected for sampling (table 1). Most of the lakes sampled are in the Arapaho and Roosevelt National Forests covering the northern part of Colorado’s Front Range. Some lakes sampled in the Western Lake Survey (Eilers et al. 1987) were resurveyed. Lakes were sampled at or near the outlet. Inlet streams were sampled at a point as near as possible to lake entry. If there was more than one inlet stream, one was selected in catchments with steep terrain, few meadows, an abundance of rock outcrops, and little soil development. These streams generally had rapid flow through distinct channels in rocky areas rather than meadows. They were likely to become nitrogen saturated 3 4 Site Lake or stream Clear Lake Kelly Lake Kelly Lake Ruby Jewel Lake Ruby Jewel Lake Lost Lake South Lost Lake South Dup Uneva Pass Lake Blodgett Lake Booth Lake Missouri Lake Savage Lake (Upper) Strawberry Lake Tuhare Lakes Blue Lake Blue Lake Blue Lake * Blue Lake * Blue Lake Talus * Caribou Lake * Caribou Lake * Caribou Lake * Caribou Lake * Caribou Lake * Columbine Lake* Columbine Lake * Columbine Lake * Crater Lake Crater Lake Crater Lake Crater Lake Crater Lake * Crater Lake * Dorothy Lake * Dorothy Lake * Gourd Lake * Gourd Lake * Gourd Lake * Isabelle Lake * Island Lake Island Lake Island Lake Island Lake * Island Lake * King Lake King Lake * King Lake * King Lake * Long Lake Wilderness Or other location Colorado State Forest Colorado State Forest Colorado State Forest Colorado State Forest Colorado State Forest Eagles Nest Eagles Nest Eagles Nest Holy Cross Holy Cross Holy Cross Holy Cross Holy Cross Holy Cross Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Inlet Inlet Inlet Inlet Outlet Inlet Inlet Outlet Inlet Outlet Inlet Outlet Inlet Outlet Inlet Outlet Inlet Inlet Inlet Inlet Outlet Inlet Outlet Inlet Inlet Outlet Inlet Inlet Outlet Inlet Outlet Outlet Inlet Outlet Inlet Outlet Outlet Outlet Inlet/ outlet Table 1. Water chemistry for lakes and streams sampled. 09/11/95 09/10/95 08/02/95 08/01/95 09/09/95 08/25/95 08/25/95 08/25/95 09/04/95 09/25/95 08/06/95 07/18/95 08/26/95 08/07/95 08/04/95 09/04/95 07/11/95 07/11/95 07/11/95 07/31/95 07/31/95 07/31/95 07/31/95 07/31/95 07/31/95 07/31/95 07/31/95 08/07/95 08/07/95 09/16/95 09/16/95 08/11/95 08/11/95 08/07/95 08/07/95 08/19/95 08/19/95 08/19/95 07/08/95 08/11/95 09/07/95 09/07/95 08/19/95 08/19/95 09/17/95 07/24/95 07/24/95 07/24/95 09/10/95 Date 6.91 6.90 6.58 6.63 7.09 6.91 6.62 7.43 6.65 6.54 6.40 5.98 6.78 6.44 5.64 5.95 5.73 6.14 6.98 7.23 6.99 6.99 7.03 7.15 6.89 7.23 7.02 6.08 6.11 6.46 6.43 6.53 6.56 6.24 6.41 7.30 7.23 7.63 6.25 6.44 6.27 6.62 7.09 6.86 6.27 6.05 6.05 6.16 6.58 pH 27.59 18.11 25.09 29.27 37.06 14.51 14.49 56.52 7.02 9.54 11.93 8.81 17.52 11.72 6.82 5.80 6.20 9.20 20.70 25.80 19.03 18.26 16.90 27.40 9.41 21.80 14.70 6.78 8.50 7.88 6.89 10.62 8.10 6.90 6.40 31.80 38.40 55.60 9.40 15.44 9.27 12.15 15.40 10.90 7.62 3.63 4.50 8.58 13.33 240.80 194.80 187.20 228.80 382.80 166.50 166.20 612.30 61.50 103.30 110.50 63.80 110.20 111.60 20.60 23.90 8.12 28.18 130.20 253.83 155.42 144.00 150.07 219.95 79.42 184.98 112.35 41.50 51.70 57.40 57.40 53.29 49.22 19.09 32.46 208.44 171.23 305.07 31.58 119.50 79.10 108.10 121.90 79.13 54.40 18.19 20.93 42.91 129.30 Conduct. ANC µs/cm µeq/l 198.71 159.09 151.97 184.56 340.38 88.85 86.70 354.09 47.46 66.07 77.27 53.66 282.60 97.26 35.83 30.05 36.73 58.63 174.65 152.89 112.23 118.96 67.61 149.90 59.38 161.88 93.81 42.32 53.44 62.84 63.03 85.43 63.37 34.03 40.92 189.12 268.11 361.63 60.33 95.53 70.35 93.87 124.55 76.70 320.56 23.10 16.92 46.73 114.42 Ca ++ µeq/l 33.73 35.93 41.56 59.88 69.80 46.77 46.37 346.12 11.62 41.88 37.13 22.28 33.91 22.42 6.75 0.00 6.42 9.62 19.25 133.10 58.74 57.01 101.02 88.85 22.29 64.25 36.28 6.75 7.49 6.98 6.17 5.18 6.09 9.38 11.43 96.00 72.97 140.18 9.46 29.36 17.32 25.30 22.13 18.76 20.90 4.36 5.10 14.73 25.02 Mg ++ µeq/l 38.24 24.94 22.46 21.85 37.25 35.41 35.18 35.76 7.69 11.87 9.44 13.68 14.84 9.77 9.40 9.40 11.44 15.05 25.92 21.84 27.93 29.71 4.52 26.10 24.31 20.75 17.88 6.31 7.31 9.22 6.85 8.48 7.79 6.35 6.70 40.71 33.75 51.15 14.31 19.96 10.91 17.23 15.66 12.88 19.23 7.44 16.49 17.96 15.93 Na + µeq/l 17.52 9.45 8.59 8.30 10.66 7.05 6.80 13.69 2.31 4.65 3.72 4.78 6.72 3.83 2.10 0.00 2.40 3.53 2.25 5.70 7.01 7.01 5.70 6.29 2.92 8.47 5.27 1.25 1.69 1.70 1.08 1.97 1.69 3.17 3.68 7.03 2.66 6.47 4.22 1.37 1.01 1.59 3.25 2.53 3.71 1.13 3.22 4.50 1.45 K+ µeq/l 2.49 1.59 0.89 0.00 2.30 1.35 1.14 1.10 0.00 0.28 0.00 0.00 0.00 1.11 0.67 0.00 1.11 1.55 0.18 0.01 0.00 0.00 0.20 1.03 0.00 0.31 0.28 0.39 0.39 0.00 0.00 0.15 0.53 0.46 1.26 1.07 0.44 0.88 0.61 0.00 0.00 0.99 0.57 0.73 1.05 0.00 2.33 1.17 0.94 NH4+ µeq/l 3.47 2.17 1.40 1.66 1.64 1.41 0.80 1.30 1.55 1.57 0.72 1.30 0.46 2.27 1.60 0.71 1.61 2.20 2.51 1.41 1.68 1.92 0.79 1.52 1.33 2.20 1.61 1.29 1.69 2.68 1.11 1.21 1.27 2.59 2.06 3.05 0.42 1.95 2.00 2.99 0.27 0.85 1.02 0.82 2.06 0.96 1.47 2.62 0.67 Cl µeq/l 1.18 0.00 2.02 15.81 5.00 0.00 0.00 11.71 0.00 0.59 3.71 3.19 5.96 6.37 12.56 7.10 20.85 17.48 17.08 15.26 8.31 13.21 3.73 3.97 3.02 17.95 7.81 5.15 4.05 0.46 0.00 12.71 3.77 8.10 6.69 17.01 0.00 2.50 15.42 8.51 5.85 0.00 4.74 7.73 0.00 1.94 8.34 6.66 0.00 NO3 µeq/l 33.10 26.30 26.22 21.09 33.79 10.76 9.62 42.68 9.40 8.48 15.40 9.47 65.78 18.38 19.56 17.12 20.02 29.85 48.74 17.77 22.75 23.85 3.33 24.73 10.48 17.87 13.06 11.38 14.59 11.46 11.29 13.41 14.12 12.50 14.62 73.70 157.47 198.30 31.52 37.01 14.86 29.16 17.02 14.52 18.05 8.81 6.56 19.35 23.91 SO4 = µeq/l 5 Site Lake or stream Long Lake Lower Coney Lake* Lower Storm Lake* Lower Storm Lake * Lower Storm Lake * No Name Lake No Name Lake Dup Red Deer Lake Red Deer Lake Red Deer Lake Red Deer Lake * Red Deer Lake * Red Deer Lake * Red Deer Lake * Round Lake Round Lake Stone Lake Stone Lake Triangle Lake* Triangle Lake * Triangle Lake * Upper Coney Lake* Upper Coney Lake * Upper Coney Lake * Upper Diamond Lake* Upper Lake Upper Lake Upper Lake Upper Storm Lake* Watanga Lake Watanga Lake Watanga Lake Watanga Lake Watanga Lake * Watanga Lake * Watanga Lake * Watanga Area Stream * U-Shaped Lake Loveland Pass Stream * Loveland Pass Stream * Loveland Pass Stream * Long Lake Lost Lake - GLEES Lost Lake - GLEES North Twin Lake South Twin Lake South Twin Lake West Glacier Lake - GLEES West Glacier Lake - GLEES Wilderness Or other location Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks Indian Peaks La Garita Loveland Pass Loveland Pass Loveland Pass Medicine Bow NF Medicine Bow NF Medicine Bow NF Medicine Bow NF Medicine Bow NF Medicine Bow NF Medicine Bow NF Medicine Bow NF Table 1. Cont’d. Outlet Inlet Outlet Outlet Inlet Outlet Inlet Outlet Inlet Inlet Outlet Outlet Outlet Inlet Outlet Outlet Inlet Inlet Inlet Outlet Outlet Outlet Outlet Outlet Inlet Inlet Outlet Inlet Inlet Outlet Outlet Outlet Inlet Outlet Outlet Inlet Outlet Inlet Outlet Inlet Inlet Outlet Outlet Inlet/ outlet 09/10/95 08/03/95 08/07/95 08/07/95 08/07/95 09/17/95 09/17/95 08/07/95 08/07/95 09/10/95 07/27/95 07/27/95 07/27/95 07/27/95 08/03/95 09/04/95 08/07/95 09/03/95 08/11/95 08/11/95 08/12/95 08/03/95 08/03/95 08/03/95 08/07/95 08/08/95 09/03/95 09/03/95 08/07/95 08/10/95 08/10/95 09/14/95 09/14/95 08/20/95 08/20/95 08/20/95 08/20/95 09/19/95 08/02/95 08/02/95 08/02/95 09/18/95 09/18/95 09/18/95 09/18/95 09/18/95 09/18/95 08/11/95 08/11/95 Date 6.54 7.07 6.86 6.57 6.95 6.10 6.09 6.36 6.36 6.40 6.92 6.08 6.63 6.57 5.96 6.22 6.35 6.53 6.18 5.89 6.74 7.00 6.77 6.97 6.99 6.38 6.33 6.27 6.60 6.52 6.51 6.76 6.78 7.57 7.30 7.33 6.93 6.56 7.32 7.02 7.52 6.66 6.52 6.64 7.97 6.84 6.73 5.43 5.96 pH 15.85 17.40 10.90 5.40 9.40 6.19 6.74 10.85 12.58 9.81 28.20 5.94 11.49 11.95 6.85 6.13 10.43 10.50 4.67 3.63 12.75 16.80 9.20 11.70 13.20 14.67 8.42 8.45 9.70 13.22 13.01 15.03 14.30 16.30 15.90 14.40 10.20 12.81 27.70 17.90 32.00 28.78 10.88 7.28 15.50 17.26 12.69 3.40 5.30 147.60 91.31 61.59 38.21 70.20 27.00 27.30 76.80 76.70 80.50 165.51 24.00 68.37 78.29 41.50 47.40 73.60 92.20 26.94 11.13 70.90 116.26 59.62 71.63 82.38 87.90 66.90 49.10 43.93 137.00 128.40 156.10 149.10 120.77 142.79 113.03 65.50 111.20 261.42 116.83 353.47 236.20 78.60 54.00 153.50 193.90 139.00 3.20 34.50 Conduct. ANC µs/cm µeq/l 139.94 139.42 67.91 41.32 74.00 35.53 34.63 70.11 66.97 76.21 196.06 27.89 74.10 81.59 41.02 37.20 65.42 70.84 30.99 20.66 102.15 161.13 85.58 102.79 76.30 76.50 56.27 61.25 63.12 82.14 73.18 106.39 100.09 90.02 101.15 77.79 64.42 94.16 207.53 101.30 235.93 174.05 70.96 47.31 127.15 156.19 117.51 18.39 33.56 Ca ++ µeq/l 28.18 16.29 27.39 11.43 23.03 12.51 12.34 21.56 21.56 21.47 56.52 8.06 19.25 21.96 12.67 6.25 17.44 13.78 2.88 2.06 6.75 12.42 7.24 8.97 38.25 22.46 6.56 11.68 25.17 23.07 23.96 28.58 27.15 23.28 22.95 21.64 12.18 29.29 80.78 23.77 145.44 106.07 41.64 26.58 34.48 48.06 37.77 5.25 14.72 Mg ++ µeq/l 19.31 16.09 10.27 14.18 14.53 8.53 9.74 18.05 15.31 13.98 31.01 13.96 19.75 18.31 10.44 12.06 10.44 12.02 5.31 2.83 12.74 11.79 10.00 12.14 10.96 11.00 10.64 11.71 8.48 57.14 56.89 0.00 65.25 59.72 56.15 54.02 26.10 30.75 36.89 46.59 21.84 42.71 13.88 10.05 24.27 26.71 19.31 2.77 6.17 Na + µeq/l 1.64 4.25 3.89 2.28 3.89 2.74 3.04 2.94 3.81 1.23 4.91 1.84 2.53 2.81 4.22 0.00 2.10 0.00 2.86 1.69 3.17 4.09 2.92 3.81 4.19 2.10 0.00 0.00 3.89 1.68 2.76 2.30 1.79 3.09 2.38 2.38 2.22 4.58 8.31 7.19 6.29 8.41 6.47 4.42 16.04 8.75 3.96 0.00 1.45 K+ µeq/l 1.11 1.21 0.00 0.00 0.09 0.00 0.00 0.67 0.67 0.00 0.03 0.00 0.00 0.00 0.39 0.00 0.39 0.00 0.12 0.93 0.09 0.14 0.00 1.27 0.00 0.39 0.00 0.00 0.00 0.84 0.00 4.82 4.70 0.44 1.76 0.01 0.34 0.00 0.55 0.00 0.00 1.33 0.28 0.00 0.44 0.00 0.00 0.00 0.00 NH4+ µeq/l 0.84 2.12 1.97 1.27 1.61 1.96 1.66 2.28 3.24 0.88 2.03 1.38 1.27 1.66 1.17 0.96 1.74 0.00 0.96 1.18 1.35 1.27 1.49 1.64 1.38 0.92 0.32 0.83 1.37 1.19 1.77 1.53 10.64 1.38 1.21 0.96 1.21 4.45 1.72 2.65 2.34 4.22 2.41 2.18 4.19 1.85 0.66 0.90 1.59 Cl µeq/l 0.51 8.82 15.13 5.85 9.48 11.48 11.40 4.14 3.58 0.00 13.19 6.56 6.55 5.79 6.62 1.13 3.13 0.99 9.55 8.74 16.34 15.16 12.40 9.48 16.18 4.77 0.91 1.97 20.05 0.26 0.00 0.00 0.00 2.45 0.00 0.00 4.52 3.07 4.92 7.84 2.44 0.67 0.99 0.00 0.00 0.00 0.00 7.23 0.00 NO3 µeq/l 32.78 49.91 14.25 14.06 16.48 8.74 9.30 25.01 25.05 23.50 71.59 11.33 25.54 25.91 12.16 12.10 17.07 19.17 5.60 5.17 20.73 39.85 25.14 36.45 14.41 20.37 15.73 15.82 15.39 12.01 13.01 16.59 21.03 16.37 12.48 14.14 16.18 19.86 43.20 15.87 13.41 19.59 33.63 18.55 22.34 14.12 11.34 4.92 10.20 SO4 = µeq/l 6 Site Lake or stream West Glacier Lake - GLEES West Glacier Lake - GLEES Abyss Lake Abyss Lake Abyss Lake Abyss Lake Dup Chicago Lake* Chicago Lake * Chicago Lake * Frozen Lake Frozen Lake Frozen Lake North Lake North Lake South Lake South Lake South Lake Dup Summit Lake* Summit Lake * Upper Middle Beartrack Lake Upper Middle Beartrack Lake Dup Upper Middle BeartracksLake Long Lake Valley Stream * Long Lake Valley Stream * Long Lake Valley Stream * Long Lake Valley Stream * Niwot * Niwot * Niwot * Niwot * Niwot * Blue Lake Blue Lake Blue Lake Camp Lake Camp Lake Carey Lake Carey Lake Carey Lake Carey Lake Area Inlet Stream * Carey Lake Area Inlet Stream * Carey Lake Area Inlet Stream * Carey Lake Area Inlet Stream * Hang Lake Hang Lake Hang Lake Iceberg Lake Iceberg Lake Island Lake Wilderness Or other location Medicine Bow NF Medicine Bow NF Mount Evans Mount Evans Mount Evans Mount Evans Mount Evans Mount Evans Mount Evans Mount Evans Mount Evans Mount Evans Mount Evans Mount Evans Mount Evans Mount Evans Mount Evans Mount Evans Mount Evans Mount Evans Mount Evans Mount Evans Niwot Ridge Niwot Ridge Niwot Ridge Niwot Ridge Niwot Ridge Niwot Ridge Niwot Ridge Niwot Ridge Niwot Ridge Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Table 1. Cont’d. Outlet Inlet Outlet Outlet Outlet Outlet Inlet Outlet Outlet Inlet Outlet Inlet Outlet Inlet Outlet Inlet Inlet Outlet Inlet Outlet Inlet/ outlet 09/14/95 09/14/95 08/04/95 09/13/95 10/04/95 10/04/95 08/02/95 08/02/95 08/02/95 08/04/95 09/13/95 10/05/95 08/07/95 09/13/95 08/07/95 09/13/95 09/13/95 08/02/95 08/02/95 09/10/95 09/10/95 08/05/95 07/08/95 07/08/95 07/08/95 07/08/95 07/07/95 07/07/95 07/07/95 07/07/95 07/07/95 08/02/95 09/08/95 09/08/95 09/10/95 09/10/95 08/06/95 09/16/95 09/16/95 08/15/95 08/15/95 08/15/95 08/15/95 08/02/95 09/08/95 09/08/95 08/06/95 09/17/95 08/06/95 Date 5.50 6.22 6.73 6.83 6.42 6.43 7.04 6.73 7.08 6.34 6.54 6.43 6.33 6.56 6.21 6.55 6.63 6.86 6.89 6.32 6.06 6.45 6.54 6.33 6.69 6.80 6.00 6.39 6.01 6.20 6.12 6.51 6.65 6.71 6.55 6.60 6.40 6.51 6.70 7.34 5.99 6.49 5.55 6.39 6.55 6.56 6.04 6.71 6.21 pH 2.82 5.03 12.70 11.97 12.12 12.81 28.60 15.50 24.10 11.92 11.53 11.66 12.37 13.33 9.83 18.46 18.65 14.10 14.64 9.57 9.23 9.58 21.30 20.90 13.40 17.80 12.58 7.92 5.30 19.80 8.72 18.74 17.45 17.73 16.33 17.50 12.04 8.39 11.65 17.00 2.40 5.30 2.20 18.46 12.67 12.92 9.25 7.33 9.86 4.40 39.90 101.90 91.30 95.90 97.50 164.29 81.69 151.18 94.20 97.60 100.80 113.40 111.30 65.90 117.00 118.20 82.46 97.75 59.60 56.70 67.60 125.48 125.98 95.81 88.01 66.62 36.40 63.23 65.56 75.56 160.00 180.60 185.40 168.60 179.90 94.00 84.00 120.40 163.04 18.39 41.55 12.87 114.70 131.50 131.30 44.40 53.60 67.60 Conduct. ANC µs/cm µeq/l 9.03 35.78 89.17 93.43 94.65 83.79 200.05 115.52 194.06 90.82 97.50 88.53 105.59 138.02 79.24 159.64 162.22 95.31 104.29 55.85 54.75 65.77 186.98 147.41 117.86 134.88 70.61 45.76 32.53 128.14 63.82 123.35 138.24 141.84 129.07 137.12 79.74 81.74 106.36 167.56 14.47 40.62 4.84 99.80 107.42 105.86 52.06 56.58 60.83 Ca ++ µeq/l 4.44 15.63 17.03 13.82 13.98 12.70 43.02 16.78 34.14 20.90 18.25 17.37 27.90 28.73 20.00 33.39 33.60 27.81 20.57 19.69 19.64 25.18 33.23 24.76 16.62 14.40 23.28 9.79 11.19 35.54 19.58 36.80 34.35 35.23 30.46 32.22 17.44 12.77 19.64 26.08 2.39 6.25 1.40 20.52 18.10 17.65 8.89 9.05 11.19 Mg ++ µeq/l 2.96 7.57 16.96 19.72 20.51 21.62 36.15 15.01 27.93 10.79 13.17 13.33 11.83 1.15 6.70 13.26 13.24 21.44 25.75 13.33 14.38 13.09 48.02 15.40 20.14 24.49 41.89 11.44 13.96 50.50 15.40 32.32 38.33 39.48 46.42 48.26 13.40 11.11 16.61 18.44 3.87 4.22 6.00 20.93 24.91 24.78 12.88 14.14 10.18 Na + µeq/l 2.15 1.92 8.08 7.16 7.09 7.46 9.64 8.75 12.84 7.21 6.66 6.07 3.79 0.00 6.37 8.85 8.88 7.60 10.20 14.86 15.39 14.96 7.60 9.28 7.60 8.00 9.69 2.81 6.19 11.53 9.41 6.61 7.25 7.55 6.73 6.37 5.06 4.91 4.74 6.85 1.97 1.53 0.79 4.11 5.03 4.97 3.81 3.18 5.50 K+ µeq/l 0.00 1.11 0.39 0.87 0.00 0.00 0.30 0.00 1.31 0.00 0.00 0.00 1.39 0.00 0.00 0.00 0.00 0.00 0.56 1.03 0.00 0.22 0.65 0.60 0.86 0.33 0.19 1.93 1.78 0.01 0.41 0.00 2.30 2.38 3.20 3.28 0.67 0.00 0.00 0.00 0.04 0.00 0.34 0.00 1.40 1.42 0.67 0.00 0.67 NH4+ µeq/l 0.61 1.27 3.14 2.96 3.12 4.31 1.89 2.26 3.33 2.13 2.63 1.91 10.13 1.77 2.04 3.54 3.57 1.89 3.07 4.30 6.17 3.85 4.68 3.38 3.75 3.41 3.95 1.64 1.97 11.59 2.12 2.27 1.70 1.80 2.23 1.53 1.86 0.00 0.00 1.38 2.37 1.35 1.02 1.97 1.14 1.04 1.92 0.00 3.75 Cl µeq/l 6.25 0.00 10.04 7.00 5.69 5.82 12.87 23.24 8.95 12.56 6.12 4.78 0.31 0.24 16.21 41.40 38.46 14.34 4.44 0.00 0.00 0.36 11.68 1.68 16.17 14.77 0.00 11.16 6.06 0.00 4.10 6.28 1.60 1.13 0.00 0.00 13.82 2.99 1.54 16.08 4.37 11.82 2.98 6.80 0.57 0.27 16.97 8.04 6.64 NO3 µeq/l 4.42 10.75 26.27 28.84 31.49 32.38 58.16 31.95 56.76 16.35 18.35 20.69 18.61 26.95 15.95 40.55 40.55 20.18 29.81 17.08 17.30 14.85 94.98 52.97 26.38 44.53 44.70 9.60 6.71 77.72 7.87 24.68 23.33 26.03 18.37 17.76 13.67 12.94 12.71 13.52 2.37 5.50 1.65 15.18 14.13 14.05 14.17 13.77 15.33 SO4 = µeq/l 7 Site Lake or stream Island Lake Island Lake Dup Little Rainbow Lake* Little Rainbow Lake* Lost Lake Lost Lake Lower Camp Lake Lower Sandbar Lake Lower Sandbar Lake Lower Sandbar Lake Lower Twin Crater Lake Lower Twin Crater Lake Lower Twin Crater Lake Lower Twin Crater Lake Lower Twin Crater Lake Lower Twin Crater Lake McIntyre Lake McIntyre Lake McIntyre Lake Number 1 Lake Number 1 Lake Number 1 Lake Number 2 Lake Number 2 Lake Number 2 Lake Number 3 Lake Number 3 Lake Number 3 Lake Number 3 Lake* Number 3 Lake* Number 4 Lake Number 4 Lake Number 4 Lake Number 4 Lake Dup Number 4 Lake* Number 4 Lake* Number 4 Lake* Rocky Hole Lake* Sugarbowl Lake Sugarbowl Lake Twin Crater Lake* Twin Crater Lake* Upper Camp Lake Upper Camp Lake Upper Sandbar Lake Upper Sandbar Lake Upper Twin Crater Lake Upper Twin Crater Lake Upper Twin Lake Wilderness Or other location Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Rawah Table 1. Cont’d. Outlet Outlet Inlet Outlet Outlet Inlet Outlet Outlet Inlet Outlet Outlet Inlet Outlet Outlet Inlet Outlet Outlet Inlet Outlet Inlet Inlet Outlet Inlet Outlet Outlet Inlet Inlet Inlet Outlet Outlet Outlet Inlet Inlet Outlet Outlet Outlet Outlet Outlet Outlet Inlet Inlet Outlet Outlet Outlet Outlet Inlet Outlet Inlet/ outlet 09/16/95 08/06/95 08/16/95 08/16/95 08/06/95 09/09/95 08/13/95 08/06/95 09/15/95 09/15/95 08/06/95 09/16/95 09/16/95 08/06/95 09/17/95 09/17/95 08/06/95 09/17/95 09/17/95 08/06/95 09/15/95 09/15/95 08/06/95 09/15/95 09/15/95 08/05/95 09/15/95 09/15/95 08/16/95 08/16/95 08/05/95 08/15/95 09/15/95 08/05/95 08/16/95 08/16/95 08/16/95 08/15/95 08/06/95 09/17/95 08/15/95 08/15/95 08/13/95 09/10/95 08/06/95 09/15/95 08/06/95 09/16/95 08/06/95 Date 6.67 6.22 7.00 6.97 6.44 6.59 6.63 6.48 6.90 6.88 6.36 6.65 6.63 6.95 6.70 6.84 6.43 6.53 6.58 6.36 6.44 6.56 6.28 6.55 6.76 6.31 6.43 6.55 6.83 6.75 5.99 7.21 6.37 5.97 6.24 6.91 7.01 7.22 6.46 6.61 6.95 6.83 6.55 6.52 6.80 6.93 6.30 6.68 6.91 pH 7.52 9.45 13.00 10.50 18.25 19.02 16.24 15.11 16.92 16.19 12.58 11.52 11.02 17.24 17.05 18.16 12.08 11.28 11.77 11.25 9.31 10.90 10.72 9.10 8.97 10.37 8.97 8.99 9.20 7.70 9.08 18.71 7.56 9.94 4.90 11.00 14.90 20.40 11.16 10.08 10.40 9.30 14.13 13.04 14.56 18.25 13.83 11.60 12.82 70.00 67.70 120.57 74.92 137.30 173.90 158.10 139.60 192.50 181.00 94.00 110.30 108.80 166.10 184.40 198.20 103.70 113.80 118.00 95.00 86.40 103.90 82.90 86.80 84.80 77.10 73.00 84.60 61.80 65.55 45.30 137.33 47.30 43.50 14.75 81.47 108.56 171.36 96.80 100.10 80.32 82.66 124.70 125.90 136.80 200.00 104.30 110.60 120.50 Conduct. ANC µs/cm µeq/l 60.81 59.33 103.39 64.42 103.84 127.50 108.44 95.01 147.77 139.69 79.74 99.81 93.84 105.64 133.05 143.49 56.39 87.29 93.81 65.42 71.07 79.59 65.42 70.19 66.61 60.83 65.41 61.06 68.86 56.64 50.60 159.18 53.75 49.20 22.75 59.53 95.31 173.65 66.97 77.97 58.03 74.00 93.40 98.99 98.50 153.79 93.26 100.65 88.12 Ca ++ µeq/l 9.25 10.37 18.92 16.12 41.80 43.69 29.38 26.66 33.24 32.27 15.88 16.46 15.70 33.74 32.15 36.64 15.88 17.72 21.88 20.74 18.11 20.19 20.74 17.59 17.42 19.91 15.79 16.00 6.91 11.93 9.63 11.11 8.94 9.63 3.04 14.81 16.45 20.65 15.06 14.70 15.96 13.57 22.67 20.72 26.66 34.39 18.27 16.38 18.27 Mg ++ µeq/l 8.83 8.87 37.93 28.53 51.89 93.87 39.91 31.71 43.65 42.65 18.88 21.04 21.34 49.80 60.16 89.30 26.79 34.63 34.79 23.97 20.34 24.72 19.44 19.67 20.14 17.79 18.77 18.62 16.70 20.18 13.40 23.75 12.06 12.61 3.13 37.93 38.63 31.93 25.66 28.32 26.92 10.27 33.77 36.97 31.71 44.39 21.14 21.02 32.28 Na + µeq/l 4.74 5.50 5.58 3.25 5.91 4.72 6.67 6.78 8.70 7.46 6.34 6.49 6.35 4.65 7.71 7.60 5.06 6.98 6.10 6.78 6.02 5.93 6.78 6.10 6.15 7.19 7.22 6.15 4.42 4.86 6.78 9.95 5.33 6.34 3.25 7.03 9.39 10.08 6.78 7.12 7.14 5.37 7.17 6.42 7.19 8.70 7.62 6.58 6.34 K+ µeq/l 0.00 0.39 0.00 0.86 1.55 6.20 0.00 0.67 2.46 2.36 0.39 0.89 0.88 1.22 4.02 4.50 0.94 1.71 1.65 0.67 0.92 1.17 0.67 0.85 0.86 0.67 0.00 0.00 0.44 0.00 1.22 0.44 0.00 0.94 2.05 0.16 1.01 0.07 0.67 1.32 0.27 0.63 0.00 2.38 0.67 2.44 1.22 0.91 0.67 NH4+ µeq/l 0.00 2.67 1.41 1.38 4.94 1.99 2.12 1.49 1.24 0.96 2.16 0.00 0.00 3.33 0.00 0.00 2.91 0.00 0.00 2.31 0.00 0.00 3.76 0.00 0.00 2.27 0.00 0.00 0.34 1.33 3.61 2.06 0.00 2.54 0.96 1.49 1.13 4.33 3.04 0.00 1.61 1.04 3.84 2.28 1.94 1.14 2.26 0.00 2.06 Cl µeq/l 0.00 6.07 5.40 6.82 0.00 0.00 0.00 0.59 0.00 0.00 12.75 4.37 0.00 0.00 0.00 0.00 0.00 0.38 0.00 4.59 0.47 0.54 6.32 1.06 0.28 8.48 9.63 0.83 2.26 9.66 17.72 16.45 13.37 17.46 8.50 11.71 8.58 13.52 4.61 0.58 7.37 12.19 0.00 0.00 1.64 0.00 15.93 4.23 0.00 NO3 µeq/l 11.18 11.37 11.64 11.79 29.15 28.63 17.84 15.29 15.60 15.16 18.86 17.97 18.13 24.17 24.77 24.33 15.85 14.38 14.79 15.16 15.19 15.32 15.53 14.70 15.18 14.85 16.82 17.86 16.33 12.50 16.19 15.83 15.58 15.03 4.65 16.29 15.00 17.52 13.53 12.31 9.85 10.56 18.95 30.99 14.84 15.16 19.95 18.22 19.64 SO4 = µeq/l 8 Upper Twin Lake Inlet Upper Twin Lake Outlet Banjo Lake Banjo Lake Dup Commanche Lake Cotton Lake Crater Lake Deadman Lake Dry Lake Upper Dry Lake Upper Dup Elaine Lake Eureka Lake Glacier Lake Goodwin Lake Hermit Lake Horn Lake Horseshoe Lake Hunts Lake Hunts Lake Dup Lilly Lake Lilly Lake Dup Lost Lake Lost Lake Dup Lower Brush Lake Lower Bushnell Lake Lower Little Sand Cr. Lake Lower Sand Creek Lake Macey Lake Middle Macey Lake Upper Mas Alto Lake Medano Lake Megan Lake North Colony Lake North Colony Lake Dup North Colony Lake Upper North Crestone Lake Rito Alto Lake San Isabel Lake Silver Lake Small Lake Above U-Shaped Lake Small Lake N of Medano Small Lake S of Blue Lake South Branch Lake South Colony Lake South Crestone Lake Stout Creek Lake (Upper) Stuart Creek Lake (Lower) U-Shaped Lake Unnamed Above Lilly Lake Rawah Rawah Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Inlet/ outlet Site Lake or stream Wilderness Or other location Table 1. Cont’d. 09/17/95 09/17/95 08/02/95 08/02/95 07/25/95 07/31/95 07/31/95 08/03/95 07/25/95 07/25/95 07/28/95 07/25/95 08/02/95 07/26/95 07/25/95 07/26/95 07/25/95 07/28/95 07/28/95 07/25/95 07/25/95 08/02/95 08/02/95 08/01/95 08/02/95 08/03/95 07/25/95 07/25/95 07/25/95 07/28/95 07/27/95 07/25/95 07/25/95 07/25/95 07/25/95 07/27/95 07/28/95 07/27/95 08/02/95 08/03/95 07/27/95 08/02/95 08/01/95 07/27/95 07/26/95 08/01/95 08/01/95 08/03/95 07/25/95 Date 6.59 6.82 6.53 6.56 7.21 7.56 6.46 6.63 6.88 6.90 6.80 7.14 6.67 7.17 7.24 6.94 7.19 6.89 6.92 7.01 7.02 6.83 6.82 7.48 7.35 6.62 6.56 6.95 6.89 6.34 6.62 6.97 6.87 6.94 7.08 7.06 6.79 6.93 6.63 6.26 6.57 6.14 7.09 7.29 6.97 6.29 6.57 6.34 7.03 pH 12.46 12.38 14.63 15.76 62.55 162.00 16.57 27.01 51.19 50.45 27.17 74.45 8.37 108.86 118.73 38.44 78.91 43.45 42.02 53.42 53.42 30.50 30.40 70.18 31.42 18.35 18.04 40.04 49.91 8.65 29.68 58.20 82.31 82.20 79.12 45.91 26.56 39.90 28.15 7.29 26.01 3.41 66.30 50.76 40.50 11.41 18.58 9.30 49.81 120.20 121.50 135.20 136.40 556.90 1048.50 128.30 206.50 502.20 493.60 248.90 554.80 79.70 612.10 761.20 388.10 620.60 376.70 374.10 467.50 469.90 272.80 271.70 673.90 267.00 153.40 148.60 407.30 381.70 66.30 229.60 447.60 572.90 569.10 757.80 467.40 224.20 391.40 213.20 53.70 194.50 19.80 654.10 418.70 406.20 77.80 152.30 75.00 466.70 Conduct. ANC µs/cm µeq/l 101.06 100.76 75.82 113.17 426.15 1361.68 136.35 210.85 424.20 411.33 196.19 636.73 47.63 1127.66 364.20 10.66 472.55 325.80 323.97 11.45 524.95 231.16 227.88 598.80 177.84 131.28 137.42 378.59 354.09 57.88 190.08 47.16 613.77 1068.09 365.74 438.06 169.99 348.24 171.43 37.36 144.05 9.48 558.88 389.62 367.65 78.83 123.40 45.16 575.85 Ca ++ µeq/l 18.41 18.18 19.96 9.95 196.50 611.36 11.80 16.90 109.03 108.78 17.40 222.67 8.50 273.64 23.16 9.39 214.77 73.29 72.73 11.10 46.74 24.41 23.69 87.97 98.50 18.49 21.20 40.07 106.64 10.40 35.08 44.19 356.31 363.71 71.61 54.89 29.54 31.55 46.29 9.78 34.46 0.00 87.71 114.63 32.46 20.78 31.74 14.36 33.82 Mg ++ µeq/l 36.88 36.99 12.06 18.44 36.32 37.39 4.72 22.89 49.28 28.49 16.73 24.32 11.85 47.23 9.25 3.11 36.97 29.75 22.37 8.99 20.57 13.86 14.50 33.20 11.35 19.96 18.59 18.31 17.96 8.48 25.75 93.04 39.15 33.67 12.02 26.08 16.83 19.34 24.64 8.90 37.04 3.30 62.28 42.98 16.77 12.17 15.23 14.86 17.96 Na + µeq/l 6.13 6.12 5.91 6.69 9.69 8.51 1.22 2.62 10.79 6.85 2.50 9.69 5.16 23.95 0.46 0.26 10.13 11.29 8.37 0.50 9.49 2.78 3.03 21.04 6.26 3.42 7.87 6.19 5.32 0.52 6.24 12.07 19.44 10.56 7.24 3.06 2.19 2.51 5.49 0.00 5.58 0.86 10.04 15.78 2.26 6.02 8.46 0.95 10.13 K+ µeq/l 1.85 1.82 0.00 2.45 1.11 2.54 0.00 0.96 2.66 1.44 2.04 1.11 0.00 0.55 0.00 0.00 0.83 1.00 0.00 0.00 2.16 0.98 0.00 1.25 0.00 1.35 0.00 2.00 0.83 0.00 0.00 26.50 15.52 1.72 0.89 1.11 0.00 0.95 0.98 0.00 0.00 0.00 1.29 2.00 4.99 0.00 0.87 0.00 2.00 NH4+ µeq/l 0.00 0.00 7.44 11.86 3.44 5.68 1.38 4.78 13.48 3.92 2.78 2.64 2.01 4.12 3.70 1.13 3.13 9.44 2.18 4.21 5.36 0.52 0.92 10.23 1.95 2.81 3.51 2.40 2.96 0.96 2.28 2.96 14.61 3.38 2.26 2.60 1.27 2.20 1.61 1.97 2.23 0.70 22.54 12.69 1.59 3.29 3.13 1.88 3.81 Cl µeq/l 0.00 0.00 7.82 7.70 5.59 28.92 16.70 2.94 11.52 10.24 0.94 21.21 0.35 17.03 6.48 4.98 13.02 18.40 18.53 12.91 9.24 3.86 3.88 0.41 5.53 0.00 5.00 0.95 12.02 10.98 17.11 6.98 21.55 21.93 15.03 5.23 5.77 4.38 7.92 0.00 3.21 1.59 0.00 11.80 0.28 9.73 5.36 0.00 17.19 NO3 µeq/l 19.55 19.71 6.21 9.06 112.73 176.40 25.29 45.77 36.77 31.81 13.35 258.03 11.02 562.79 526.77 16.80 231.94 27.21 27.20 89.30 86.49 11.64 11.94 68.35 21.22 44.66 26.82 16.80 125.55 12.69 20.17 182.31 318.14 282.33 139.23 17.23 13.88 10.80 26.97 8.34 22.18 4.95 25.56 73.77 12.24 17.72 30.51 10.49 43.47 SO4 = µeq/l 9 Upper Brush Lake Upper Bushnell Lake Upper Lake of The Clouds Upper Lake of The Clouds Dup Upper Little Sand Creek Lake Upper Sand Creek Lake Upper Willow Lake Venable Lake Lower Venable Lake Upper West Creek Lake Wild Cherry Lake Wild Cherry Lake Dup Glacier Lake Small Lake S. of Blue Lake Small Lake S. of Blue Lake Lake Due South of Ute Lake Due South of Ute Lake Due South of Ute - Dup Middle Ute Lake Middle Ute Lake Small Pond Above Trout Lake Small Pond Above Trout Lake Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo Sangre De Cristo South San Juan South San Juan South San Juan Weminuche Weminuche Weminuche Weminuche Weminuche Weminuche Weminuche Inlet/ outlet * water analysis by UC, all others analyzed by USFS, RMS Lab Detection Limits, USFS, RMS Lab: Site Lake or stream Wilderness Or other location Table 1. Cont’d. 08/01/95 08/02/95 07/26/95 07/26/95 08/03/95 07/25/95 07/26/95 07/25/95 07/25/95 08/01/95 07/25/95 07/25/95 09/05/95 09/05/95 09/05/95 08/05/95 09/13/95 08/05/95 08/08/95 09/13/95 08/05/95 08/11/95 Date 7.11 6.79 7.26 7.53 6.47 7.01 6.73 6.79 6.74 6.25 7.01 7.05 6.34 5.90 6.04 5.94 5.90 6.29 6.12 6.21 6.20 6.08 pH 74.97 12.25 121.49 120.01 13.43 28.18 27.27 33.24 36.85 12.42 49.66 50.38 7.52 4.16 4.01 5.30 4.88 5.36 6.81 7.94 3.64 4.79 747.60 78.50 787.40 789.40 110.10 195.10 223.40 267.50 313.70 65.80 440.00 445.00 65.30 26.20 26.80 31.30 19.60 32.20 43.80 41.90 24.10 26.90 Conduct. ANC µs/cm µeq/l 0.680 346.86 80.72 1034.93 1043.91 108.06 187.36 184.07 188.82 227.69 61.92 434.79 428.05 55.64 21.86 20.39 33.40 33.53 33.11 44.11 43.71 19.47 23.60 Ca ++ µeq/l 0.353 64.63 23.44 325.04 329.15 12.31 25.78 31.52 84.67 105.33 25.07 93.23 92.62 9.21 3.04 2.83 13.99 9.55 14.02 21.16 18.19 7.55 7.32 Mg ++ µeq/l 0.281 27.57 11.58 35.97 34.80 13.87 17.19 7.76 46.32 39.97 25.56 22.43 22.77 12.48 6.44 6.57 4.11 16.66 5.75 5.45 8.31 3.41 9.40 Na + µeq/l 0.256 11.47 6.85 8.80 9.90 1.98 5.80 1.03 19.72 12.30 9.64 5.99 6.31 6.07 3.91 4.02 3.29 7.08 10.26 1.95 1.46 2.13 4.27 K+ µeq/l 0.674 0.92 3.05 1.11 8.87 0.00 1.03 0.00 4.10 2.94 1.15 2.15 2.92 0.00 0.00 0.00 0.00 0.61 0.00 0.00 3.55 0.00 0.00 NH4+ µeq/l 0.402 3.53 3.72 4.26 3.38 3.73 2.97 1.41 19.41 8.72 9.18 1.97 2.27 0.78 0.00 0.00 18.79 2.18 56.88 9.54 2.08 1.55 2.16 Cl µeq/l 0.244 0.73 11.14 4.98 11.77 10.53 4.58 16.43 0.00 2.97 10.35 0.49 0.50 0.00 0.00 0.00 0.00 0.59 0.00 0.00 4.28 0.00 0.61 NO3 µeq/l 0.373 80.14 21.19 526.77 519.69 26.49 37.02 11.57 40.29 52.53 26.04 84.89 83.99 10.24 5.98 6.21 7.82 10.12 8.23 15.24 17.28 2.75 7.50 SO4 = µeq/l Samples were collected in 250 ml brown plastic bottles that were prewashed and filled with deionized water. They were emptied just before sampling. The bottles were labeled onsite using permanent markers with sample location, date, time, crew member names, and whether a lake or stream was sampled. Lake samples were collected as far from the shore as possible without disturbing the lake bottom. Stream samples were collected slightly upstream from the stream outlet as far into the stream as possible or at midstream if the stream was narrow. Bottles were rinsed three times with sample water then filled completely and capped. The samples were immediately placed in a small Styrofoam or thermal cooler containing a frozen icepack to keep them cold during transport. Protocols called for the collection of one duplicate sample from every 10th site and one deionized water field blank from every 20th site. Field blanks were unused, unemptied sample bottles taken to the field and returned unopened for analysis. Lakes chosen for duplicate samples were selected at random. Color photos were taken of the sample collection site and the surrounding landscape. A field data sheet containing lake, stream, watershed, and weather information was completed on site. Samples and field data sheets were shipped by overnight mail or hand delivered to the Rocky Mountain Forest and Range Experiment Station laboratory for chemical analysis. University of Colorado samples were analyzed in their laboratory. because of limited opportunity for contact with soils of sufficient exchange capacity to retain nitrates. The lakes and streams in the Rawah and Indian Peaks Wilderness areas were sampled for water chemistry in late July or early August. A second sample was collected in early September. If the system had detectible levels of nitrate in any of the samples, it was considered nitrogen saturated. Although this study was a synoptic survey of lakes in Indian Peaks and Rawah Wilderness areas, data from additional lakes sampled during 1995 using the same protocols and the same sample collection window are also included in this report. These additional lakes are: five lakes in the Medicine Bow National Forest in southeastern Wyoming; 45 lakes and streams studied by the University of Colorado in the Indian Peaks and Rawah Wilderness areas in the Arapaho and Roosevelt National Forests; and approximately 80 lakes surveyed as synoptic or long-term monitoring in the Sangre De Cristo, Mt. Evans, Weminuche, South San Juan, La Garita, and Holy Cross Wildernesses in Colorado. Sampling protocols Sample collection followed precise protocols to avoid contamination and to standardize where and how samples were collected. Two training sessions were held before sampling to instruct field crews on the proper procedures. One lake water sample was collected from each site. An inlet-stream water sample was also collected if suitable inlets were present and access was possible for the Rawah Wilderness, Indian Peaks Wilderness, and Medicine Bow Mountain range lakes. The first sample was collected near the lake outlet and the second from a first order stream inlet to the lake. The sampling point was on the lake or stream edge on solid ground or rock to minimize water disturbance. Areas of wet or bog-type soil where foot pressure might disturb the lake or stream water were avoided. It was very important not to disturb the lake or stream bottom to avoid sample contamination. Plastic gloves, prewashed in distilled water, dried, and stored in zipped plastic bags, were worn during sample collection. Analyses Samples were filtered (0.045 mm) in the laboratory and analyzed for cations (Ca2+, Mg2+, Na+, K+, NH4+) and anions (Cl-, NO3-, SO42-, PO43-) using ion chromatography. Sample pH, alkalinity, and conductivity were also determined in the laboratory. Data were examined for any unusual outlier of anions, cations, pH, alkalinity, or conductivity, and for ionic balances. A sample was considered nitrogen saturated if the nitrate concentration was 0.244 µeq/l or higher, which was the detection limit for the laboratory analytical methods at the Rocky Mountain Forest and Range Experiment Station laboratory. 10 RESULTS AND DISCUSSION This study provides the most extensive database in existence regarding the chemical composition of high-elevation lakes and streams in the Front Range of the Rocky Mountains of Colorado and southeastern Wyoming (table 1). In addition, this study identifies lakes that might be susceptible to acidification and/or nitrogen loading from atmospheric deposition. The data presented here will be analyzed further to relate surface water chemistry to landscape characteristics. Lakes with very low ANC and high levels of nitrate will be further examined in follow up studies. Most lakes examined in this study had low ANC (figure 1), suggesting sensitivity to acidification. More than 10% of the lakes were below 50 µeq/l ANC and can be considered very sensitive. The Sangre de Cristo Wilderness, where more than two-thirds of the lakes had greater than 200 µeq/l ANC, is an exception. However, 15% of the lakes in this Wilderness were below 100 µeq/l ANC and can be considered sensitive. Phosphate data are not presented because it was not detected in any of the lakes. This reflects the detection limit of the analysis rather than the presence or absence of phosphate. Variable levels of phosphate were likely present at low concentrations; phytoplank- Figure 1. Statistical box plot of acid neutralizing capacity in µeq/l (micro equilvalents per liter) for lakes sampled in 1995. The solid rectangle is the range of 50% of the values; the dot is the median value; the dashed lines are the range of all observations; and the open circles are the outlier values. ton population dynamics can respond to phosphate levels below the detection level of some analytical techniques. There were detectable1 levels of nitrate in many of the lakes (figure 2), suggesting that they could 1 Some of the data presented in table 1 are below the determined detection limits. The reader should be aware of the uncertainty of those data. Detection limits were calculated following Environmental Protection Agency methods as “three times the standard deviation of 10 nonconsecutive reagent or calibration blank analyses” (EPA 1987). Figure 2. Nitrate concentration in wilderness lakes by sampling date. 11 be nitrogen saturated. Further experimentation is necessary to verify saturation. The data also show that samples collected early in the season had nitrate concentrations higher (figures 2 and 3) than those collected later in the season. The earlier samples were probably influenced by the late snowpack runoff. Nitrates were frequently higher in lake inlet streams to than outlet streams (figure 4). This may be a reflection of the shorter residence time in streams versus lakes and the decreased opportunity for exchange in streams with soil and biota compared to lakes. Chemical composition will be correlated with catchment physical characteristics in future manuscripts. Landscape habitat and soil type were identified from topographic and soils maps and were field verified. The data will be stratified and analyzed by latitude/longitude, elevation, geology, soil type, terrain features, and landscape characteristics to determine if these factors influence nitrogen saturation. Sites with particularly low ANC and particularly high nitrate levels will be resampled for water chemistry at more frequent intervals, and sampled for phytoplankton species and biomass to verify timing, extent, and source of any possible saturation. Further analyses will be Figure 4. Box plot of nitrate concentration in µeq/l (micro equilvalents per liter) for lakes sampled in 1995 comparing difference between lake inlets and outlets (inlet minus outlet). The solid rectangle is the range of 50% of the values; the dot is the median value; and the dashed lines are the range of all observations. conducted to compare stream versus lake data for nitrate content. Phosphorus concentrations also will be closely monitored. Changes in nitrogen input to wilderness ecosystems can affect the nutrient balance and species assemblages of these systems. High-elevation mountainous aquatic ecosystems in the Front Range are particularly sensitive to these changes. Understanding the susceptibility and response of aquatic ecosystems to the direct input of atmospheric deposition is important for wilderness managers maintaining ecosystems for future use. The data from this study provide information on the impact of pollutants from point sources that are located near wilderness areas. LITERATURE CITED Aber, J.D.; Nadelhoffer, K.J.; Steudler, P.; Melillo, J.M. 1989. Nitrogen saturation in northern forest ecosystems. BioScience 39: 378-386. Arno, S.F.; Hammerly, R.P. 1984. Timberline. Mountain and Arctic Forest Frontiers. The Mountaineers. Seattle. 304 pg. Baron, J.S.; Ojima, D.S.; Holland, E.A.; Parton, W.J. 1994. Analysis of nitrogen saturation potential in Rocky Mountain tundra and forest: implications for aquatic systems. Biogeochemistry 27: 61-82. Figure 3. Box plot of nitrate concentration in µeq/l (micro equilvalents per liter) for lakes sampled in 1995 comparing the difference between early and late sampling dates (early minus late). The solid rectangle is the range of 50% of the values; the dot is the median value; and the dashed lines are the range of all observations. 12 Bowman, W.D. 1992. Inputs and storage of nitrogen in winter snowpack in an alpine ecosystem. Arctic and Alpine Research 24: 211-215. Campbell, D.H.; Clow, D.W.; Ingersoll, G.P.; Mast, M.A.; Spahr, N.E.; Turk, J.T. 1995. Processes controlling the chemistry of two snowmeltdominated streams in the Rocky Mountains. Water Resources Research 31(11): 2811-2821. Eilers, J.M.; Kanciruk, P.; McCord, R.A.; Overton, W.S.; Hook, L.; Blick, D.J.; Brakke, D.F.; Kellar, P.E.; DeHaan, M.S.; Silverstein, M.E.; Landers, D.H. 1987. Western Lake Survey. Phase I. Characteristics of Lakes in the Western United States. Volume II. Data Compendium for Selected Physical and Chemical Variables. EPA/ 600/3-86/054b. U.S. Environmental Protection Agency, Washington, D.C. Environmental Protection Agency. 1987. Handbook of methods for acid deposition studies. Laboratory analyses for surface water chemistry. EPA 600/4-87/026. U.S. Environmental Protection Agency, Washington, D.C. Irving, P. (ed). 1991. Acidic Deposition: State of Science and Technology. Summary Report of the U.S. National Acid Precipitation Assessment Program. Washington, D.C. Kendall, C.; Campbell, D.H.; Burns, D.A.; Shanley, J.B.; Silva, T.R.; Chang, C.C.Y. 1995. Tracing sources of nitrate in snowmelt runoff using the oxygen and nitrogen isotopic compositions of nitrate. Biogeochemistery of Seasonally SnowCovered Catchments. Proceedings of a Boulder Symposium, July 1995. pg 339-347. IAHS Publ. No 228. Landers, D.H.; Eilers, J.M.; Brakke, D.F.; Overton, W.S.; Kellar, P.E.; Silverstein, M.E.; Schonbrod, R.D.; Crowe, R.E.; Linthurst, R.A.; Omernick, J.M.; Teague, S.A.; Meier, E.P. 1987. Western Lake Survey. Phase I. Characteristics of Lakes in the Western United States. Volume I. Population Descriptions and Physioco-Chemical Variables. EPA/600/3-86/054a. U.S. Environmental Protection Agency, Washington, D.C. Morris, D.P.; Lewis, W.M. Jr. 1988. Phytoplankton nutrient limitation in Colorado mountain lakes. Freshwater Biology 20: 315-327. Musselman, R.C. (technical coordinator). 1994. The Glacier Lakes Ecosystem Experiments Site. Rocky Mountain Forest and Range Experiment Station, RMS General Technical Report RM-249. Fort Collins, Colorado. Smith, W.H. 1992. Air pollution effects of forest processes. Chapter 11 in Baker, J.R. and Tingey, D.T. eds. Air Pollution Effects on Biodiversity. Van Nostrand Reinhold, New York. Weber, W.A. 1976. Rocky Mountain Flora. Colorado Associated University Press. 479 pg. Williams, M.W.; Caine, N.; Baron, J.; Sommerfeld, R. 1993. Is nitrogen saturation occurring in the Colorado Front Range Abstracts. LTER All Scientists Meeting and International Summit. September 18-24, 1993. Estes Park, Colorado. LTER Network Office, Seattle, Washington. Williams, M.W.; Baron, J.S.; Caine, N.; Sommerfeld, R.; Sanford, R. Jr. 1996. Nitrogen saturation in the Rocky Mountains. Environmental Science & Technology 30: 640-646. Williams, M.W. 1994. Institute of Arctic and Alpine Research, University of Colorado. Unpublished Data. 13 ACKNOWLEDGMENTS This study was initiated as a cooperative effort between the Arapaho and Roosevelt National Forests and the Rocky Mountain Forest and Range Experiment Station. The National Forest was interested in a synoptic study of high-elevation lake water chemistry and the Research Station was interested in sensitivity of high-elevation lakes to atmospheric deposition. The University of Colorado has been studying water nitrogen saturation in Front Range aquatic ecosystems. A study plan was written and approved by USDA Forest Service researchers. Sample analyses for most lakes were funded by the respective National Forests or by Forest Service Research. The University of Colorado funded their sample analyses. We gratefully acknowledge the assistance of the volunteers who attended sample collection training and collected the water and stream samples for this study. We acknowledge personnel in the Rocky Mountain Forest and Range Experiment Station Water Analysis Laboratory who filtered and analyzed the National Forest and Forest Service Research samples. We thank Christi Gordon, Leslie Dobson, and Andrea Sears for use of data from their National Forests in this report. We acknowledge Jill Baron, National Biological Service, for assisting in one of the sample collection training sessions. We are particularly grateful to students Tim Platt-Mills, University of Colorado at Boulder and Yale University, who collected the University of Colorado samples, and Darrel Jury, Colorado State University, who compiled the list of Arapaho and Roosevelt National Forests Wilderness lakes to sample and assisted in sample collection and data compilation. We thank Rudy King for advice and assistance with data analysis. The United States Department of Agriculture (USDA) prohibits discrimination in its programs on the basis of race, color, national origin, sex, religion, age, disability, political beliefs and marital or familial status. (Not all prohibited bases apply to all programs.) Persons with disabilities who require alternative means for communication of program information (braille, large print, audiotape, etc.) should contact the USDA Office of Communications at (202) 720-2791. To file a complaint, write the Secretary of Agriculture, U.S. Department of Agriculture, Washington, D.C. 20250, or call (202) 720-7327 (voice) or (202) 720-1127 (TDD). USDA is an equal employment opportunity employer. Printed on recycled paper U.S. Department of Agriculture Forest Service Rocky Mountain Forest and Range Experiment Station Rocky Mountains The Rocky Mountain Station is one of eight regional experiment stations, plus the Forest Products Laboratory and the Washington Office Staff, that make up the Forest Service research organization. RESEARCH FOCUS Research programs at the Rocky Mountain Station are coordinated with area universities and with other institutions. Many studies are conducted on a cooperative basis to accelerate solutions to problems involving range, water, wildlife and fish habitat, human and community development, timber, recreation, protection, and multiresource evaluation. Southwest RESEARCH LOCATIONS Research Work Units of the Rocky Mountain Station are operated in cooperation with universities in the following cities: Great Plains Albuquerque, New Mexico Flagstaff, Arizona Fort Collins, Colorado Laramie, Wyoming Lincoln, Nebraska Rapid City, South Dakota *Station Headquarters: 240 W. Prospect Rd., Fort Collins,CO 80526