Richard Pratt

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Plant Breeding at The Ohio State University

Presenter:

Richard Pratt

INTRODUCTION

Dept. of Horticulture and Crop Science @The Ohio State University - OARDC, Wooster, OH 44691

Ohio is a leading producer of both agronomic and horticultural crops. OSU maize and wheat breeding programs have existed for over 100 years. The soybean breeding program started prior to 1920 and tomato breeding was initiated in the 1940s.

OSU breeding programs support traditional plant agriculture and emerging bioproducts industries, conduct fundamental and applied research, and contribute to undergraduate, graduate, and post-graduate education. All crop breeding programs at OSU emphasize product quality and resistance to biotic stresses (primarily phytopathogens).

Maize, wheat, and tomato research programs are based at the Ohio Agricultural

Research and Development Center (OARDC) Wooster Campus. The soybean research and ornamentals programs are located at both the Columbus and Wooster campuses. The wheat breeding program cooperates closely with the USDA-ARS

Regional Soft Wheat Quality Laboratory

(http://www.ars.usda.gov/main/)

and the maize breeding program cooperates closely with the ARS Corn and Soybean

Research Group

(http://www.ars.usda.gov/main/)

(OSU-ARS Maize Virology

Program). The tomato breeding program cooperates closely with the Van der

Knaap laboratory (tomato fruit developmental biology;

http://oardc.osu.edu/ vanderknaap/default.htm)

and the Dept. of Food Sciences. The soybean breeding program cooperates closely with the Ohio Crop Performance Trials (OSU Extension) and ARS Corn and Soybean Research Group (above ref.). Germplasm preservation and distribution of ornamental crops (priority genera: Begonia, Coreopsis, Lilium,

Phlox, Rudbeckia and Viola) is carried out by the USDA Ornamental Plant

Germplasm Center (Columbus;

http://opgc.osu.edu/

).

Status of OSU-OARDC plant breeding investment

The primary investment by the state of Ohio is through a line item in the state budget to the OARDC. Substantial support has been received from the Ohio

Soybean Council (soybean), the Midwestern Canners Assoc. (tomato). Recently, an 11.5 million dollar award was made by the Ohio Department of Development to create the Ohio Bioproducts Innovation Center (OBIC). Funds from OBIC have been used to enhance high-throughput genotyping at the Molecular and Cellular

Imaging Center at the Wooster Campus. Direct OARDC support for technicians has declined precipitously in the last several years and additional operating costs are now borne directly by breeding programs.

Wheat Breeding (Clay Sneller)

• Find genetic solutions to the problems that limit profitability of wheat production in Ohio and beyond

• Develop cultivars for farmers

• Develop enhanced germplasm for breeders

• Determine genetics of key traits such as disease resistance, flour improvement and adaptation

• Develop efficient mapping and marker–assisted selection (MAS) methods

Soybean Breeding (Steve St. Martin)

• Development of varieties

– High yield, resistance to Phytophthora

– Modified oil for industrial and food applications

– Specialty varieties for tofu production

• Quantitative Genetics & Breeding Methods

– Genetic gain by stages

– Use of exotic germplasm

– Statistical methods

– Involved in mapping and genomics projects

Maize Breeding (Rich Pratt)

• QTL Discovery and Molecular Breeding

– Maize Streak Virus, Gray Leaf Spot,

Northern Corn Leaf Blight

• Value-added grain compositional traits

(protein, oil, starch)

• Selection of maize genotypes with improved

grain nutritional (carotenoids,

QPM) and physical characteristics

• Introgression of desirable traits in exotic

(temperate/tropical) breeding populations

Tomato Breeding (Dave Francis)

• Developing breeding strategies that optimize discovery and introgression of new genes from wild relatives

• Development of genetic resources to study bioactive compounds and human nutrition, mechanisms of disease resistance, and the discovery of molecular markers

• Understand the genetic processes that are under selection during the domestication of plants and the breeding of elite varieties

• Leverage emerging DNA sequence data and comparative biology to gain insight into the fundamental genetic changes that have been imparted through targeted selection in plant breeding

Part 5.

Plant breeding curriculum

Core plant breeding curriculum:

The OSU graduate plant breeding and genetics curriculum provides graduate education leading to understanding of the concepts, principles, and methodology of crop improvement. Graduate education is achieved by offering courses in plant breeding concepts and methodology, experimental design, data analysis, and population genetics.

Additional core courses in underlying disciplines will contribute to knowledge of genetic and physiological processes and environmental influences that result in the expression of phenotypic variation.

Following are the core and allied courses for students pursuing graduate degrees in the breeding and genetics areas of the Horticulture and Crop Science graduate program:

Required Core Courses

M.S. Program

• Genetics and Molecular Biology – Mol Gen 500

• Introduction to Biochemistry – Biochem 511

• Field Crop Breeding – H&CS 625

• Data Analysis I and II – Stat 528 and 529

• Research Methods – H&CS 604

• Tech. of Experimental Design – H&CS 887

or Analysis and Interpretation of Biological Data – Mol Gen 650

Increased local funds have been made available on a competitive basis and OSU breeders have been successful in obtaining them. Examples include the OARDC

Research Enhancement Competitive Grants Program (SEEDS;

http://www.

oardc.ohio-state.edu/seeds/

) and AgBioScience Innovation Grants programs

(AgBio;

http://www.oardc.ohiostate.edu/abigp/

). SEEDS supports diverse programs that will foster enhanced research productivity and

AgBIO promotes an entrepreneurial culture within the agbioscience university setting. OSU breeding programs are now encouraged to commercialize their research products.

Multidisciplinary research is also strongly valued and supported, as illustrated by long-standing programs such as the Maize Virology Program and new initiatives sponsored by

SEEDS and AgBio grants.

Part 1.

Breeding efforts by crop

Breeding Program

Objectives:

Conduct problem oriented research and achieve a high level of technology integration across all commodities .

Ornamentals (Michelle Jones, John Finer, Dan Struve, Pablo Jourdan)

• Selection of improved Sweetbay Magnolia and Ornamental Pear Annuals

• Enhancement of Petunia flower longevity using transgenic approaches

(

http:/oardc.osu.edu/joneslab/

).

USDA Ornamental Plant Germplasm Center (David Tay)

Establish an herbaceous ornamental plant genebank as an integrated

component of the USDA National Plant Germplasm System (NPGS)

Build a network of cooperators from the horticultural industry,

universities, botanic gardens & arboreta, USDA, crop specific societies

and individual gardeners

Establish a system that promotes industry oriented collaborative

research to enhance germplasm utilization

Institute academic programs and industrial short courses in

germplasm conservation

Part 2.

Breeding efforts by activity

Part 3.

Graduate student enrollment

Recent (6)

Current (9)

Part 4.

Job placement of recent graduates:

(3) University

(1)National Programs

(1) Peace Corps

(1) Industry

Ph.D. Program

The above courses plus the following:

• Advanced Plant Breeding – H&CS 825

• Plant Genetics and Molecular Biology – Mol Gen 622

• and Plant Genetics and Molecular Biology – Mol Gen 623

• or Systems of Genetic Analysis – Mol Gen 700

• or DNA Transactions – Mol Gen 701

• or Genetics of Animal Populations – An Sci 721

• or Agricultural Genomics – Plant Pathology 703

Part 6.

Institutional philosophy

The Ohio State University breeding programs have long been known for their responsiveness to local, national and international needs. OSU plant breeders value and engage interdisciplinary cooperators to form effective, problem solving teams. Overarching themes such as product quality and host-resistance have been conducive to, and have benefited from, these cooperative relationships. OSU breeders have stressed the importance of integrating genetic research with their breeding programs so that they might simultaneously advance academic and practical goals. In an environment of “molecularization” and “privatization,” core breeding programs have retained their commitment to the land-grant “missionorientation.”

We are now at a crossroads between our desire to continue variety and germplasm development and the institution’s ability to support that effort. New strategies to more closely link core competencies of the breeding programs with market opportunities and value-added product development are being implemented.

These strategic goals must be pursued with an increasing mindfulness of the environmental context. The redirection of breeding programs will encounter obstacles (e.g., policies, culture, reward systems) beyond the funding limitations.

How emergent priorities will mesh with the demands of traditional audiences also will be revealed. It will be necessary to examine and address the possible impacts

(both positive and negative) of these new directions.

We conclude the following:

• Public breeders can and should respond to the challenge of increasing farm profitability

• Closer integration of biotechnological, breeding, and social sciences will become increasingly critical

• Efficiency can be gained by identifying important problems, and developing specific technologies, in areas that are under-served by the private sector

• Increased integration of breeding activities into commercialization streams is inevitable

• The future of applied breeding at OSU will lie in successfully developing funding mechanisms at the seam of the basic/applied research interface

• A stable base of public funding is vitally needed to educate scientists needed by the private sector and maintain our responsiveness to the needs of our local constituents

• Public breeders can and should direct their research toward helping to ensure that agriculture is increasingly in harmony with the environment

• We must continue to exploit our unique strengths in breeding/germplasm development in order to continually improve our graduate education and research programs

For more information, contact:

Dr. Richard Pratt, Professor

Dept. of Horticulture and Crop Science

OSU-OARDC

1680 Madison Avenue

Wooster, OH 44691-4096

Tel: 330-263-3972

Fax: 330-263-3887 e-mail: pratt.3@osu.edu

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