ABIO 340 Virology
Syllabus
Dr. Wm. Jackson
Spring 2013
Instructor Information
Instructor:
William H. Jackson, Ph.D.
Office:
SBDG 201A
Office Hours:
By Appointment
Telephone:
641-3601
Email:
Billj@usca.edu
Course Information
Lecture:
TTh, 9:25-10:40PM in SBDG
200
Text:
Viruses: Biology, Application, and
Control, 1st Edition by Harper
Laboratory:
M, 9:00-11:40AM in SBDG
108
Laboratory notebook,
Scantron® Sheets
Four (4) Semester Hours
Text:
Writing Papers in the Biological
Sciences, 4th Edition by McMillan
Supplies:
Credit:
Additional Information:
Blackboard
Course Description: Virology is a four credit hour course for biology majors, which covers the basic principles of
virology. Topics covered will include viral diseases, virus-cell interactions, and patterns of viral replication. The
course will primarily cover viruses directly involved in human disease.
Course Objectives: This course will introduce the student to the underlying principles of virology. On completion
of this course, the student will be expected to
1. Describe the replication strategies of DNA and RNA viruses
2. Describe the pathogenesis of viral diseases in humans
3. Describe strategies to prevent and combat viral diseases
4. Conduct a literature search and prepare a written review of a specific viral family
Attendance Policy: Students are expected to attend all lecture and laboratory sessions. In this regard, the instructor
may impose a penalty for absences in excess of 25% of regularly scheduled class (lab or lecture) meetings by
assigning an “F” in the course. Absences, neither excused not unexcused, absolve the student from meeting class
assignments. Because of their nature, weekly quizzes and laboratory exercises cannot be made up.
Disability Statement: If you have a physical, psychological, and/or learning disability that might affect your
performance in this class, please contact the Office of Disability Services, B&E 134, (803) 643-6816, as soon as
possible. The Office of Disability Services will determine appropriate accommodations based on documentation.
Policy for Portable Electronic Devices: The use of any portable electronic devices, including cell phones, pagers,
MP3 players, iPods, etc., during class is not allowed for any reason unless prior approval has been given to a student
from the instructor or unless required for the course. If you are planning to have any of these devices in class, they
must be turned off and stowed away for the duration of the class period. If you use a portable electronic device
during a test, quiz, or other assessment, you are eligible to receive a failing grade on that assignment.
Methods of Presentation and Evaluation: Information will be presented through lectures, class discussion, and
laboratory exercises using appropriate visual aids and laboratory equipment. Your final grade will be determined as
shown below.
You will be expected to endorse the USCA HONOR PLEDGE on every assignment: 'On my honor as a University
of South Carolina Aiken student, I have neither given nor received any unauthorized aid on this
assignment/examination. To the best of my knowledge, I am not in violation of academic honesty'.
-1-
ABIO 340 Virology
Syllabus
Description
Three 1-hour exams
Final Exam
Weekly quizzes
Research Paper
Literature reviews
Laboratory reports
Poster presentation
Laboratory quizzes
Class participation
Dr. Wm. Jackson
Spring 2013
Points
Percentage
300
100
100
150
60
110
40
50
90
30%
10%
10%
15%
6%
11%
4%
5%
9%
Comments
10% each
Comprehensive final exam
Covering material from the previous 1-2 weeks
Literature review of a virus family
Review of the primary literature (10 pts each)
Written report of laboratory assignments
Summary of laboratory results
Covering laboratory content
Lecture (60 pts); Lab (30 pts): 2 pts per meeting
Letter grades will be based on the total accumulated points and will be assigned as follows: A>900; B>800; C>650;
D>550; F<550.
Writing Assignments: Below is a brief overview of the writing assignments you will be responsible for during the
semester. More complete descriptions and tasks will be provided separately. Written products will be evaluated
based on one of two rubrics that will be provided with each assignment. Multiple revisions are encouraged during
the writing process, and should be based on both peer reviews and conferences with the instructor.
Research paper. Your research paper will consist of a full description of a particular virus family. The specific
family will be chosen by the student. Revisions will be allowed (and expected) based on peer reviews and
conferences with the instructor.
Important Dates:
Assignment
Due Date
First draft with bibliography due: hardcopy and electronic copy to Turnitin
March 22, 2013
First draft reviews begin
March 26, 2013
Reviews due
April 1, 2013
Final paper due
April 15, 2013
Research summaries. You will be assigned six primary research articles from your research paper’s bibliography to
summarize. Revisions will be allowed (and expected).
Important Dates:
Assignment
Start Date
Due Date
Revision Due
Resch Paper Review 1
January 28, 2013
February 1, 2013
February 7, 2013
Resch Paper Review 2
February 4, 2013
February 8, 2013
February 14, 2013
Resch Paper Review 3
February 11, 2013
February 15, 2013
February 21, 2013
Resch Paper Review 4
February 18, 2013
February 22, 2013
February 28, 2013
Resch Paper Review 5
February 25, 2013
March 1, 2013
March 7, 2013
Resch Paper Review 6
March 4, 2013
March 8, 2013
March 14, 2013
-2-
ABIO 340 Virology
Syllabus
Dr. Wm. Jackson
Spring 2013
TENTATIVE LABORATORY SCHEDULE
WEEK
1
DATE
Jan 14
2
3
Jan 21
Jan 28
4
Feb 4
5
Feb 11
6
Feb 18
7
Feb 25
8
Mar 4
9
10
Mar 11
Mar 18
11
Mar 25
12
Apr 1
13
Apr 8
14
15
16
Apr 15
Apr 22
Apr 29
The Molecular Biology of the Human Immunodeficiency Virus
Introduction to research: the literature search and bibliography; Assignment of topic
and discussion of lab procedures and proper maintenance of lab records
MLK Day – No Classes
Assignment: 5 papers on HIV gene function with 250-word review (Introduction)
Experimental design – PCR and Primer design
Assignment: Methods (oligo design)
Molecular Biology Tools – cloning, transformation, colony screening, gel
electrophoresis
Amplification of HIV gene sequences
Assignment: Methods (PCR)
Cloning of PCR product – rapid ligation and transformation
Assignment: Methods (cloning); Results (PCR)
Analysis of transformation and Molecular Biology Tools – Southern blot, Western
Blot, and ELISA
Assignment: Results (cloning)
Spring Break – No Classes
Colony analysis – prepare miniprep DNA
Assignment: Methods (colony analysis)
Miniprep analysis – restriction enzyme digest and gel electrophoresis
Assignment: (1) Results (colony analysis)
Data preparation – plasmid maps, restriction maps
Assignment: appropriately labeled plasmid map
Discussion – What does it all mean?
Assignment: discussion
Poster preparation
Poster preparation and wrap up
Poster session
Virology poster session: Each group will prepare a poster that describes their virology lab project. A poster
template will be provided. A discussion on how to prepare a research poster can be found in chapter 10 in your
supplemental text. The poster session will be held on Monday, April 29 at 1PM in the Science Building. During this
time, each group will be expected to remain next to their poster to explain their work and answer questions. The
audience will be members of the Biology and Geology faculty, as well as students, and any other invited guests.
Laboratory Evaluation. Each written lab assignment will by evaluated on a 15-point scale providing a maximum
of 150 points. The poster and poster session will make up the remaining 100 points of the 250 possible points in lab.
-3-
ABIO 340 Virology
Syllabus
WEEK
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Final
DATE
Jan 15
Jan 17
Jan 22
Jan 24
Jan 29
Jan 31
Feb 5
Feb 7
Feb 12
Feb 14
Feb 19
Feb 21
Feb 26
Feb 28
Mar 5
Mar 7
Mar 12
Mar 14
Mar 19
Mar 21
Mar 26
Mar 28
Apr 2
Apr 4
Apr 9
Apr 11
Apr 16
Apr 18
Apr 23
Apr 25
May 2
Dr. Wm. Jackson
Spring 2013
TENTATIVE LECTURE SCHEDULE
TOPIC
An Introduction to Virology
An Introduction to Virology
Virus Structure and Infection
Virus Structure and Infection
Virus Structure and Infection
Virus Classification and Evolution
Virus Classification and Evolution
Virus Classification and Evolution
Exam I
Virus Replication
Virus Replication
Virus Replication
Immune Response and Evasion
Immune Response and Evasion
Immune Response and Evasion
Vaccines and Vaccination
SPRING BREAK – No Classes
Exam II
Vaccines and Vaccination
Vaccines and Vaccination
Antiviral Drugs
Antiviral Drugs
Emergence, Spread, and Extinction
Emergence, Spread, and Extinction
Emergence, Spread, and Extinction
Exam III
Viral Replication Strategies
Viral Replication Strategies
Viral Replication Strategies
Final Exam at 8:00AM in H&SS 103
-4-
CHAPTER
1
1
1
2
2
2
3
3
3
4
4
4
5
5
5
6
6
8
8
8
Appendix I
Appendix I
Appendix I
Cumulative