Variety Mixtures for Reduced Input Barley, 2006 M. J. Ottman Summary Variety mixtures may lessen competition among plants and reduce effects of stress particularly in environments where resources are limiting. Mixtures of four barley varieties were grown under low input conditions at the Maricopa Agricultural Center. The barley varieties seeded were Barcott, Solum, Solar, and an experimental low input line designated Entry 9. The highest yields were not obtained with mixtures in this experiment, but rather when the varieties were grown alone. Barcott and Entry 9 decreased yield when part of the mixture more than Solum or Solar. When Solum was grown in a mixture rather than alone, test weight and lodging were improved, but yield was decreased. Introduction It is possible to obtain higher yields with mixtures of crops than with individual crops planted alone. Intercropping, as this practice is called, is not practical with mechanical harvesting and is used mainly in the tropics with crops that are harvested by hand. If a single species is planted, such as wheat or barley, varieties with differing characteristics may be mixed and harvested mechanically. Variety mixtures can outperform solid plantings of single varieties if the varieties in the mixtures are sufficiently different in characteristics including height, rooting pattern, or maturity. The problem with crops where all plants are similar is that they compete more intensely with each other for resources such as water, light, and fertilizer. An example of a successful variety mixture in Arizona is Aldente, a mixture of WestBred 881 and Turbo that combined some of the quality of WestBred 881 with the yield potential of Turbo. Variety mixtures may lessen competition among plants and reduce effects of stress particularly in environments where resources are limiting. Procedure Mixtures of four barley varieties were grown under low input conditions at the Maricopa Agricultural Center. The barley varieties included in the mixtures were: 1) Barcott – a high input barley, 2) Solum – a low input barley, 3) Solar – a low input barley, and 4) Entry 9 – a low input 2-row barley (the other three varieties are 6-row barleys). Various proportions of these barley varieties were included in 15 mixtures, and sown at rates of 1000 or 2000 seeds per plot or 12 or 24 seeds/ft2 (assuming seeded area of 49 inches by 20 ft) corresponding to about 40 and 80 lbs seed per acre (Table 1). The experimental design consisted of 2 simplexes (groups of 15 mixtures) and 2 initial densities. The experiment was conducted in Field 1, Border 18 at the Maricopa Agricultural Center during the 2005-2006 growing season. The soil type was a Casa Grande sandy loam. The previous crop was bermudagrass. The surface 6 inches of soil was sampled before planting and contained 31.0 ppm NO3-N and 5.4 ppm P. The mixtures were planted in 5 ft x 20 ft plots on November 30, 2005. Seven rows spaced 7 inches apart were planted. The irrigation system was border flood, an irrigation to germinate the seed was applied on December 2, and an additional irrigation was applied on February 17. Fertilizer was applied preplant as ammonium phosphate sulfate (16-20-0) at a rate of 48 lbs N/acre and 60 lbs P2O5/acre. Nitrogen fertilizer was applied at of rate of 50 lbs N/acre as urea (46-00) on February 17 prior to the irrigation. Stand counts were made on December 29 from four areas with dimensions of 2 ft long and 5 rows wide (23.3 ft2). On January 16 at the 5-leaf stage, 20 plants were sampled per plot and the plant area and total dry weight were measured. Whole plants including crowns were dug up and removed on April 19 from an area 2 rows wide by 16 Forage and Grain Report (P-154) October 2007 90 feet long. The individual varieties in each mixture were separated based on distinguishing characteristics. Barcott is short, Solum has a rough awn, Solar has a smooth awn, and Entry 9 has 2-row head type. The following was recorded for each variety: plant height, number of plants, stems per plant (from 10 plants), head per plant (from 10 plants), total plant weight, and grain weight. On May 11, lodging was noted and a small plot combine was used to harvest the grain. Barley grain samples were cleaned by running through a head thresher. Test weight was determined from these cleaned samples using a 1-pint container. Seed weight was determined from a 10 g sample of grain. Discussion The barley varieties were chosen based on differences in plant characteristics. Barcott is the shortest variety, and the others are roughly similar in height (Table 6). Solum is the earliest in maturity, and the others mature around the same time (Table 2). Our hypothesis was that differences in these characteristics would reduce plant competition and potentially increase grain yield. However, the highest yields were not obtained with mixtures in this experiment, but rather when the varieties were grown alone. Three of the highest four yields were obtained when the varieties were grown alone (Table 3). This could have occurred because Entry 9 dragged down the yield of the others. This variety had low grain yield in this study, but also was a vigorous plant with a low grain to straw ratio that may have competed with the other varieties without contributing a commensurate share of grain yield. Another possibility is that the varieties had a negative impact on each other in a mixture, such as a slight allelopathic reaction. One of the benefits of mixtures is that negative characteristics of certain varieties can be lessened by the presence of other varieties. For example, negative characteristics of Solum is low test weight and high lodging potential. Solum planted alone had a test weight of 46.5 lbs/bu and lodging of 40%, but when planted with Solar predominantly (10% Barcott, 40% Solum, 40% Solar, 10% Entry 9), the test weight was increased to 48.0 lbs/bu and lodging decreased to 15%. However, the yield was also reduced by 14%. Stand and initial growth was least for Barcott and greatest for Entry 9 (especially at the higher seeding rate), and intermediate for Solum and Solar (Table 4). Thus, the composition of the mixtures refers to the amount seeded and not necessarily the amount established. At harvest, there were fewer plants of Barcott and more plants of Entry 9 in the mixtures than were seeded, while the percentage of Solum and Solar seeded and harvested were similar (Table 5). The same trend was measured for stem number (Table 7) and head number (Table 8). Entry 9 was also the best competitor in the mixture in terms of total plant yield, Barcott was the worst competitor, and Solar was more competitive than Solum (Table 9). However, since Entry 9 had a low grain to straw ratio, it did not dominate the mixtures in terms of grain yield (Table 10). Solar contributed most to the grain yield of the mixtures, followed by Entry 9, Solum, and Barcott. Mixtures may have potential for increased yield compared to single varieties, but this research has not provided any evidence for this effect. We have shown that some varieties do better than others in a mixture. Barcott, the short variety probably did poorly because it was shaded by the taller varieties even though it was earlier in maturity. Entry 9 was not productive when planted alone, and decreased yield of the mixtures it was part of. Perhaps the key to increased productivity of mixtures is extensive testing and careful selection of components of the mixture. Acknowledgments Financial support for this project was received from the Arizona Grain Research. The technical assistance of Mary Comeau, Mike Sheedy, and Tony Gomez is greatly appreciated. Forage and Grain Report (P-154) October 2007 91 Table 1. Seeding rate and number of seed of each barley variety for the mixtures. Seeding rate seeds/plot seeds/ft2 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 1000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 2000 12 12 12 12 12 12 12 12 12 12 12 12 12 12 12 24 24 24 24 24 24 24 24 24 24 24 24 24 24 24 Mixture lbs Percentage of seed/acre Barcott/Solum/Solar/Entry9 40 40 40 40 40 40 40 40 40 40 40 40 40 40 40 80 80 80 80 80 80 80 80 80 80 80 80 80 80 80 Mixture composition (seeds/plot) Barcott Solum Solar Entry 9 700 100 100 100 250 400 400 400 100 100 100 1000 0 0 0 1400 200 200 200 500 800 800 800 200 200 200 2000 0 0 0 100 700 100 100 250 400 100 100 400 400 100 0 1000 0 0 200 1400 200 200 500 800 200 200 800 800 200 0 2000 0 0 100 100 700 100 250 100 400 100 400 100 400 0 0 1000 0 200 200 1400 200 500 200 800 200 800 200 800 0 0 2000 0 100 100 100 700 250 100 100 400 100 400 400 0 0 0 1000 200 200 200 1400 500 200 200 800 200 800 800 0 0 0 2000 70/10/10/10 10/70/10/10 10/10/70/10 10/10/10/70 25/25/25/25 40/40/10/10 40/10/40/10 40/10/10/40 10/40/40/10 10/40/10/40 10/10/40/40 100/0/0/0 0/100/0/0 0/0/100/0 0/0/0/100 70/10/10/10 10/70/10/10 10/10/70/10 10/10/10/70 25/25/25/25 40/40/10/10 40/10/40/10 40/10/10/40 10/40/40/10 10/40/10/40 10/10/40/40 100/0/0/0 0/100/0/0 0/0/100/0 0/0/0/100 Table 2. Heading, anthesis, and maturity for the barley varieties at two seeding rates. Seeding rate (lbs/acre) Barcott Solum Solar Entry 9 Heading 40 3/4 3/3 3/1 3/17 Anthesis 40 3/4 3/3 3/1 3/17 Maturity 40 4/13 4/5 4/14 4/14 Heading 80 3/4 3/2 3/2 3/17 Anthesis 80 3/4 3/2 3/2 3/17 Maturity 80 4/13 4/7 4/14 4/14 Forage and Grain Report (P-154) October 2007 92 Table 3. Grain yield, test weight, seed weight, and lodging at harvest for barley mixtures. 40 lbs seed/acre 80 lbs seed/acre Average Percentage of Grain Test Seed Lodg- Grain Test Seed Lodg- Grain Test Seed LodgBarcott/Solum/Solar/Entry9 yield weight weight ing yield weight weight ing yield weight weight ing % lbs/A lbs/bu mg % lbs/A lbs/bu mg % lbs/A lbs/bu mg % 70/10/10/10 10/70/10/10 10/10/70/10 10/10/10/70 25/25/25/25 40/40/10/10 40/10/40/10 40/10/10/40 10/40/40/10 10/40/10/40 10/10/40/40 100/0/0/0 0/100/0/0 0/0/100/0 0/0/0/100 All 3388 3415 3307 2985 3496 3254 3522 2823 3684 2904 3092 3657 3926 3791 2743 3332 45.6 46.1 49.2 50.2 49.0 46.7 47.7 47.3 48.1 47.6 49.3 44.3 45.6 51.1 49.5 47.8 34.5 34.5 33.7 34.1 35.2 33.6 33.5 31.9 35.5 33.0 33.7 32.5 37.6 37.7 31.1 34.1 10 5 40 5 10 0 10 15 15 10 2 5 20 7 0 10 3200 3576 3711 2554 3415 3469 3684 2850 3334 3307 3469 3603 4195 4114 2501 3399 46.3 46.6 48.8 49.5 47.7 47.5 49.5 47.4 47.8 48.8 48.8 43.1 47.4 49.9 48.7 47.8 31.9 34.2 33.8 32.0 33.1 34.7 34.4 30.3 31.2 34.2 31.8 29.1 36.8 34.9 28.1 32.7 50 0 25 0 10 20 15 2 15 10 5 5 60 10 10 16 3294 3496 3509 2770 3455 3361 3603 2837 3509 3106 3280 3630 4060 3953 2622 3366 46.0 46.4 49.0 49.9 48.3 47.1 48.6 47.3 48.0 48.2 49.1 43.7 46.5 50.5 49.1 47.8 33.2 34.3 33.8 33.0 34.1 34.1 34.0 31.1 33.4 33.6 32.7 30.8 37.2 36.3 29.6 33.4 30 3 33 3 10 10 13 9 15 10 4 5 40 9 5 13 Table 4. Stand on December 29, and plant weight and PAI (plant area index) on January 16 at the 5-leaf stage. 40 lbs seed/acre 80 lbs seed/acre Average Plant Plant Plant Percentage of Stand Stand Barcott/Solum/Solar/Entry Stand weight PAI weight PAI weight PAI 9 % plants/ft2 lbs/A plants/ft2 lbs/A plants/ft2 lbs/A 70/10/10/10 10/70/10/10 10/10/70/10 10/10/10/70 25/25/25/25 40/40/10/10 40/10/40/10 40/10/10/40 10/40/40/10 10/40/10/40 10/10/40/40 100/0/0/0 0/100/0/0 0/0/100/0 0/0/0/100 All 8.3 9.7 9.9 11.1 8.0 7.9 10.0 9.9 8.3 10.5 11.4 7.4 8.3 10.1 9.9 9.4 293 355 453 462 355 282 313 465 461 439 498 186 336 406 371 379 Forage and Grain Report (P-154) October 2007 0.63 0.79 1.07 1.06 0.79 0.64 0.74 1.13 1.17 1.07 1.20 0.44 0.83 1.07 0.99 0.91 11.5 15.0 15.4 20.4 15.9 12.7 16.9 14.2 13.7 16.0 15.5 13.3 12.6 12.3 21.2 15.1 395 582 808 682 528 533 568 423 375 468 514 231 375 507 646 509 1.10 1.49 2.26 1.85 1.42 1.42 1.54 1.22 0.99 1.31 1.48 0.68 0.95 1.47 1.85 1.40 9.9 12.3 12.6 15.8 12.0 10.3 13.5 12.0 11.0 13.3 13.4 10.4 10.4 11.2 15.6 12.2 344 469 630 572 442 408 441 444 418 453 506 209 355 457 508 444 0.86 1.14 1.66 1.45 1.10 1.03 1.14 1.17 1.08 1.19 1.34 0.56 0.89 1.27 1.42 1.15 93 Table 5. Number of plants of each variety at harvest as a percentage of the total mixture. Plant number of each variety at harvest as a percentage of total 40 lbs seed/acre 80 lbs seed/acre Average Percentage seeded of BarBarBaBarcott/Solum/Solar/Entry cott Solum Solar Entry9 cott Solum Solar Entry rcott Solum Solar Entry 9 9 9 % % % % 70/10/10/10 10/70/10/10 10/10/70/10 10/10/10/70 25/25/25/25 40/40/10/10 40/10/40/10 40/10/10/40 10/40/40/10 10/40/10/40 10/10/40/40 100/0/0/0 0/100/0/0 0/0/100/0 0/0/0/100 Average 49 7 2 5 9 23 22 17 5 8 4 100 0 0 0 14 15 59 8 5 25 32 13 8 34 22 9 0 100 0 0 21 14 6 75 6 26 23 44 11 40 12 29 0 0 100 0 26 23 27 15 85 40 22 21 64 21 58 58 0 0 0 100 39 49 6 2 7 7 23 15 16 8 10 3 100 0 0 0 13 13 66 8 6 21 40 49 7 20 24 3 0 100 0 0 23 16 9 70 7 22 14 14 9 57 10 32 0 0 100 0 24 23 20 20 80 50 24 21 69 15 56 62 0 0 0 100 40 49 7 2 6 8 23 19 16 6 9 3 100 0 0 0 13 14 63 8 5 23 36 31 7 27 23 6 0 100 0 0 22 15 8 72 7 24 18 29 10 49 11 31 0 0 100 0 25 23 23 18 82 45 23 21 66 18 57 60 0 0 0 100 40 Table 6. Plant height of each variety in the various mixtures at harvest. Plant height of each variety at harvest 40 lbs seed/acre 80 lbs seed/acre Average BaBarBarPercentage seeded of Barcott/Solum/Solar/Entry cott Solum Solar Entry9 cott Solum Solar Entry rcott Solum Solar Entry 9 9 9 % inches inches inches 70/10/10/10 10/70/10/10 10/10/70/10 10/10/10/70 25/25/25/25 40/40/10/10 40/10/40/10 40/10/10/40 10/40/40/10 10/40/10/40 10/10/40/40 100/0/0/0 0/100/0/0 0/0/100/0 0/0/0/100 All 24 24 24 21 23 24 24 30 30 28 23 29 ------25 29 30 33 31 34 33 32 36 37 36 32 --38 ----33 34 34 35 35 35 36 38 40 40 39 36 ----34 --36 Forage and Grain Report (P-154) October 2007 37 34 36 36 36 36 37 40 36 39 35 ------36 37 22 23 21 23 20 22 27 26 24 25 22 26 ------23 30 30 32 27 33 33 37 32 31 37 34 --30 ----32 33 34 35 33 33 36 40 38 34 36 36 ----33 --35 32 35 37 31 31 37 39 33 34 35 37 ------36 35 23 23 22 22 21 23 25 28 27 27 22 27 ------24 29 30 33 29 33 33 34 34 34 37 33 --34 ----33 33 34 35 34 34 36 39 39 37 37 36 ----33 --36 34 34 36 34 33 37 38 36 35 37 36 ------36 36 94 Table 7. Number of stems of each variety at harvest as a percentage of the total mixture. Stem number for each variety at harvest as a percentage of the total 40 lbs seed/acre 80 lbs seed/acre Average Percentage seeded of BarBarBaBarcott/Solum/Solar/Entry cott Solum Solar Entry9 cott Solum Solar Entry rcott Solum Solar Entry 9 9 9 % % % % 70/10/10/10 10/70/10/10 10/10/70/10 10/10/10/70 25/25/25/25 40/40/10/10 40/10/40/10 40/10/10/40 10/40/40/10 10/40/10/40 10/10/40/40 100/0/0/0 0/100/0/0 0/0/100/0 0/0/0/100 Average 44 5 2 3 7 26 19 10 4 6 2 100 0 0 0 12 20 60 7 3 17 26 9 4 29 14 9 0 100 0 0 18 14 7 68 6 23 20 49 9 48 9 30 0 0 100 0 26 21 29 22 89 54 28 23 77 20 71 58 0 0 0 100 45 44 6 1 4 4 16 12 12 5 7 2 100 0 0 0 10 15 52 5 4 14 39 58 5 14 16 3 0 100 0 0 20 16 9 74 6 19 12 12 6 62 9 27 0 0 100 0 23 26 33 20 85 63 33 19 77 20 68 69 0 0 0 100 47 44 6 2 3 5 21 15 11 4 6 2 100 0 0 0 11 18 56 6 4 15 32 33 4 21 15 6 0 100 0 0 19 15 8 71 6 21 16 31 7 55 9 28 0 0 100 0 24 24 31 21 87 58 31 21 77 20 70 63 0 0 0 100 46 Table 8. Number of heads of each variety at harvest as a percentage of the total mixture. Head number for each variety at harvest as a percentage of the total 40 lbs seed/acre 80 lbs seed/acre Average BaBarBarPercentage seeded of Barcott/Solum/Solar/Entry cott Solum Solar Entry9 cott Solum Solar Entry rcott Solum Solar Entry 9 9 9 % % % % 70/10/10/10 10/70/10/10 10/10/70/10 10/10/10/70 25/25/25/25 40/40/10/10 40/10/40/10 40/10/10/40 10/40/40/10 10/40/10/40 10/10/40/40 100/0/0/0 0/100/0/0 0/0/100/0 0/0/0/100 Average 1 2 3 4 5 6 7 8 9 10 11 100 0 0 0 12 43 6 2 3 8 30 22 11 4 5 3 0 100 0 0 18 22 60 7 4 19 24 8 4 31 13 11 0 0 100 0 27 Forage and Grain Report (P-154) October 2007 16 6 73 7 23 21 53 9 46 10 31 0 0 0 100 43 20 28 18 87 50 26 17 75 19 73 56 100 0 0 0 11 48 6 1 5 4 18 11 14 6 12 2 0 100 0 0 21 14 52 5 6 11 46 61 4 16 18 3 0 0 100 0 24 16 9 77 8 22 14 13 8 60 9 32 0 0 0 100 43 22 33 16 82 64 22 15 75 19 62 63 100 0 0 0 12 45 6 2 4 6 24 17 13 5 8 3 0 100 0 0 20 18 56 6 5 15 35 34 4 24 16 7 0 0 100 0 26 16 8 75 7 22 18 33 8 53 9 31 0 0 0 100 43 95 Table 9. Total plant yield of each variety at harvest as a percentage of the total mixture. Total plant yield for each variety at harvest as a percentage of the total 40 lbs seed/acre 80 lbs seed/acre Average Percentage seeded of BarBarBaBarcott/Solum/Solar/Entry cott Solum Solar Entry9 cott Solum Solar Entry rcott Solum Solar Entry 9 9 9 % % % % 70/10/10/10 10/70/10/10 10/10/70/10 10/10/10/70 25/25/25/25 40/40/10/10 40/10/40/10 40/10/10/40 10/40/40/10 10/40/10/40 10/10/40/40 100/0/0/0 0/100/0/0 0/0/100/0 0/0/0/100 Average 38 5 1 2 4 19 13 9 2 5 2 100 0 0 0 9 18 64 4 5 25 31 14 7 33 17 11 0 100 0 0 21 21 7 74 12 30 27 57 14 47 18 36 0 0 100 0 31 23 25 21 81 41 23 17 71 18 60 51 0 0 0 100 39 39 6 1 5 5 15 9 11 5 7 2 100 0 0 0 10 17 63 5 6 18 42 14 8 21 23 4 0 100 0 0 20 21 10 76 11 28 18 59 14 60 12 42 0 0 100 0 32 23 20 18 78 50 26 19 68 14 58 52 0 0 0 100 39 39 6 1 3 5 17 11 10 4 6 2 100 0 0 0 9 18 64 5 6 21 36 14 7 27 20 8 0 100 0 0 21 21 8 75 11 29 23 58 14 53 15 39 0 0 100 0 31 23 23 19 80 45 24 18 69 16 59 52 0 0 0 100 39 Table 10. Grain yield of each variety at harvest as a percentage of the total mixture. Grain yield for each variety at harvest as a percentage of the total 40 lbs seed/acre 80 lbs seed/acre Average BaBarBarPercentage seeded of Barcott/Solum/Solar/Entry cott Solum Solar Entry9 cott Solum Solar Entry rcott Solum Solar Entry 9 9 9 % % % % 70/10/10/10 10/70/10/10 10/10/70/10 10/10/10/70 25/25/25/25 40/40/10/10 40/10/40/10 40/10/10/40 10/40/40/10 10/40/10/40 10/10/40/40 100/0/0/0 0/100/0/0 0/0/100/0 0/0/0/100 Average 41 5 1 3 5 18 13 10 2 6 2 100 0 0 0 10 22 72 6 9 32 38 17 9 38 21 16 0 100 0 0 25 22 7 81 17 33 28 59 18 48 21 41 0 0 100 0 34 Forage and Grain Report (P-154) October 2007 16 16 11 72 31 17 12 63 12 52 41 0 0 0 100 31 41 6 1 5 6 14 9 12 5 10 3 100 0 0 0 10 20 70 6 10 23 49 17 10 24 29 6 0 100 0 0 24 24 10 81 16 31 19 61 20 61 13 50 0 0 100 0 35 15 14 12 69 41 18 13 58 9 48 42 0 0 0 100 31 41 6 1 4 5 16 11 11 4 8 2 100 0 0 0 10 21 71 6 9 27 44 17 9 31 25 11 0 100 0 0 25 23 8 81 17 32 24 60 19 54 17 45 0 0 100 0 35 15 15 12 70 36 17 13 61 11 50 42 0 0 0 100 31 96