Upland Cotton Variety Evaluation in Graham County, 2003 E.R. Norton and L.J. Clark Abstract A field trial was established during the 2003 growing season as part of the statewide Upland Cotton Variety Testing Program. This trial was located in Thatcher with Dennis Layton Farms as the cooperator. The location was one of eleven around the state. A total of twelve varieties were entered from six cooperating seed companies. Varieties included DP655BR, DP555BR, DP449BR, and DP5690R from Delta and Pine Land Company; FM989BR, FM991R, and FM991BR from Fiber Max; ST5303R and ST5599BR from Stoneville; Riata from CPCSD; AG3601 from Arizona Cotton Growers Association; and SCX-7 from Salcot. The twelve varieties were planted in a randomized complete block design with three replications. Individual plots consisted of 4, 38” rows extending the full length of the irrigation run (1250 ft.). Plant measurements were collected throughout the season to evaluate growth and development characteristics of each variety. Yield and fiber quality data was collected at the end of the season by harvesting and weighing each individual experimental unit. Sub-samples were collected for fiber quality analysis. Percent emergence data indicated differences in seedling vigor and stand establishment. Percent emergence ranged from a high of nearly 90% (Fiber Max FM989BR) to less than 50% (Delta and Pine DP555BR). Even with the low percent emergence for DP555BR an adequate stand was achieved for each variety and did not significantly impact final yield. Significant differences were observed in final lint yields with Fiber Max FM991BR producing the highest yield at 1690 lbs. lint per acre and Fiber Max FM989BR producing the lowest yield at 1292 lbs. lint per acre, a difference of approximately 400 lbs. Delta and Pine DP655BR has been the standard variety planted in the valley for several years. The only variety producing more lint than DP655BR was FM991BR. Lint value calculated using premium/discounts for fiber quality resulted in FM991BR with the highest value at $952/acre. All varieties had overall premiums except for AG3601which was discounted due to high fiber micronaire. Results from this evaluation indicate that FM991BR appears to be an additional variety from which growers have to choose that has the potential to perform very well in the Upper Gila River valley. Introduction Variety selection is one of the most important decisions that a producer can make that will directly affect the outcome of the year’s crop. With the development of transgenic technologies, the overall number of varieties available has dramatically increased over the recent years making the decision of which variety to plant more difficult. There are several sources available to help the producer determine which variety best suits a particular farm including seed company variety trials conducted around the state. In an effort to contribute in an unbiased manner the University of Arizona conducts a statewide Upland Cotton variety testing program that involves locations around the state to which seed companies may submit varieties for evaluation. Most tests are located on grower-cooperator fields under normal production practices for that region. These regional tests are conducted by individual county extension agents with cooperators selected from local growers. Arizona Cotton Report (P-138) May 2004 104 Materials and Methods Twelve varieties were selected by the cooperating seed companies in 2003 and planted in a randomized complete block design with four replications. Plots were four - 38” rows wide and extended the full length of the irrigation run of 1250 feet. Plots were planted on 28 April 2003 with 638 heat units (86o/55oF thresholds) accumulated since January 1. Table 1 provides agronomic information for this study. Stand counts were collected on two separate dates shortly after emergence to evaluate percent emergence and seedling vigor of each variety. Plant measurements were taken on approximately fourteen-day intervals throughout the growing season including plant height, number of mainstem nodes, number of aborted or missing sites, and the number of nodes above the top, first position fresh bloom. Yield estimates were made by harvesting the entire four-row plot, for each replication, at the end of the season and weighing each plot with a boll buggy equipped with load cells. Sub-samples were taken from each plot for fiber quality and percent lint estimates. Premiums or discounts were calculated based upon fiber quality for each variety from data developed for the CCC loan schedule. The overall value of the crop was also determined by multiplying lint yield by the adjusted price determined from fiber quality discounts or premiums. Results Stand count information collected shortly after emergence reveals significant differences among varieties. Percent emergence estimates were made on 16 May and 3 June. Values from a low of near 50% to just below 90%. Table 2 contain analysis of variance data for the percent emergence data. Some variation was also experienced among the sample dates. Figure 1 shows seedlings/acre for each variety on the two separate sampling dates. Some varieties experienced sharp declines in plant population in the 18 day interval between evaluations. DP449BR and Salcot SC-7 both experienced over a 10% decrease in plant population between sample dates (Figure 2). Planting conditions for this trial were optimum with minimum soil temperatures never dropping below 60oF during the fourteen day period following planting. Height to node ratio (HNR) provides a measurement of crop vigor and is calculated by dividing the total plant height (inches) by the number of mainstem nodes. The values of HNR are plotted against normal baselines for each of the twelve varieties in Figure 3. HNR trends consistently trended along normal baselines throughout the season. Fruit retention measurements provide an indication of fruit load and yield potential of a particular variety. FR levels for each of the twelve varieties are plotted against normal baselines in Figure 4. All twelve varieties performed in a similar manner with respect to fruit load. All varieties tracked at or above the center (normal) baseline throughout the season. End of season FR levels were still considerably higher than 50% indicating good yield potential for all twelve varieties. The number of nodes above the top, first position fresh flower (NAWF) is a good indicator of progression of a particular variety towards physiological maturity or ‘cut-out’. Levels of NAWF for each of the twelve varieties are plotted in Figure 5 against normal baselines. Levels were consistently below normal throughout the entire season. However, this situation is not uncommon when the fruit load is consistently high as was experienced by this crop (Figure 4). Final lint yields ranged from a low of 1292 lbs/acre to the highest lint yield at 1690 lbs/acre, a difference of nearly 400 lbs/acre. Lint yield data is presented in Table 3 and Figure 6. A new variety to Graham County performed very well in 2003, Fiber Max FM991BR. Fiber Max variety FM989BR which has performed very well this region in past years did not perform as well in 2003. This was in part due to the presence of unopened bolls toward the top of the plant. Another variety that performed extremely well in 2002 that did not do as well in 2003 is Deltapine DP555BR. Deltapine DP655BR has been a standard variety planted in this region for many years. Year in and year out DP655BR consistently performs well. To evaluate each of the varieties with respect to the DP655BR standard a percent relative yield based on DP655BR was Arizona Cotton Report (P-138) May 2004 105 calculated. A graph showing percent relative yield and percent relative value are shown in Figures 7 and 8 respectively. Only FM991BR performed at a level higher than the DP655BR standard with respect to lint yield and value. A premium or discount was calculated for each of the twelve varieties based on fiber quality results (Table 3) from high volume instrument (HVI) analysis. This premium/discount value is shown in Table 3 and Figure 9. Fiber Max FM991BR and Deltapine DP449BR received the highest premium of over 400 points. The only variety receiving a discount based on fiber quality was Arizona Cotton Growers Association AG3601. Figures 10-12 show each of the fiber quality parameters and the discount/premium levels associated with each parameter. Results from this test demonstrate the range of quality varieties from which to choose for growers in the Upper Gila River Valley. It is important to remember that performance in one year is not necessarily an indication of how that variety will perform in other years. It is always a good idea to experiment with an unfamiliar variety in small acreages at first in an effort to become familiar with it prior to planting a large number of acres to a new variety. Arizona Cotton Report (P-138) May 2004 106 Table 1. Agronomic information for the Graham County Upland Cotton Variety Test, 2003. Planting Date 28 April Final Irrigation Date 26 September Harvest Date 30 October Total Number of Irrigation 7 Total N Applied 83 lbs. N General Soil Texture Grabe Clay loam Table 2. Percent emergence data for each of the twelve varieties on two separate sample dates for the Graham County Upland variety trial, 2003. Percent Emergence Variety 16 May 3 June FM989BR 88.3 a1 81.2 a1 DP449BR 81.7 ab 68.6 bcd Riata 75.0 abc 73.8 ab ST5599BR 70.1 bcd 73.4 abc Salcot SCX-7 69.1 bcd 51.6 ef DP655BR 68.0 bcd 73.0 abc DP5690R 68.0 bcd 61.0 de FM991BR 66.9 cd 62.0 cde ST5303R 60.3 de 61.9 cde AG3601 60.3 de 59.7 de FM991R 59.8 de 57.1 def DP555BR 47.3 e 48.1 f LSD2 14.0 11.6 OSL3 0.0002 0.0001 C.V.4(%) 14.4 12.5 1 Means followed by the same letter are not significantly different according to a Fisher’s LSD means separation test. LSD: Least Significant Difference. 3 OSL: Observed Significance Level. 4 CV: Coefficient of Variation. 2 Arizona Cotton Report (P-138) May 2004 107 Table 3. Lint yield and fiber quality characteristics for each of the nine varieties planted in 2003. Company FiberMax Stoneville Delta and Pine FiberMax Delta and Pine Delta and Pine Salcot Delta and Pine ACGA Stoneville CPCSD FiberMax LSD0.052 OSL3 CV (%)4 Variety FM991BR ST5303R DP655BR FM991R DP5690R DP555BR SCX-7 DP449BR AG3601 ST5599BR Riata FM989BR Lint Yield (lbs/Acre) 1690 a1 1661 a 1661 ab 1632 abc 1618 abc 1593 abc 1517 bcd 1496 cde 1398 def 1395 def 1363 ef 1292 f 144 0.0001 6.0 Percent Lint 36.0 a 35.8 a 35.0 a 34.1 a 35.5 a 36.0 a 33.9 a 34.8 a 32.3 a 34.8 a 35.5 a 32.3 a NS 0.1218 5.0 Micronaire 4.3 a 4.4 a 4.4 a 4.5 a 4.7 a 4.4 a 4.4 a 4.3 a 5.1 a 4.3 a 4.7 a 4.3 a NS 0.0797 5.4 Fiber Length (100ths) 1.10 a 1.04 a 1.07 a 1.05 a 1.07 a 1.06 a 1.08 a 1.10 a 1.08 a 1.07 a 1.08 a 1.08 a NS 0.1035 2.5 Fiber Quality Staple Fiber Length Strength (32nds) (g/tex) 35.2 a 32.6 a 33.5 a 30.3 a 34.5 a 30.5 a 33.5 a 28.8 a 34.5 a 30.9 a 34.2 a 30.1 a 34.5 a 31.0 a 35.2 a 31.7 a 34.5 a 32.0 a 33.5 a 30.8 a 34.5 a 31.8 a 34.7 a 31.9 a NS NS 0.2394 0.1690 2.7 5.2 Uniformity Index 81.2 ab 80.0 abc 80.5 abc 79.0 c 81.2 ab 79.7 bc 80.0 abc 81.5 ab 81.0 ab 80.2 abc 81.7 a 81.5 ab 1.8 0.0488 1.4 Premium/ Discount5 (points) 430 81 334 85 281 231 360 423 -13 280 354 380 1 Means followed by the same letter are not significantly different according to a Fisher’s LSD means separation test. LSD: Least Significant Difference. 3 OSL: Observed Significance Level. 4 CV: Coefficient of Variation. 5 Average premium or discount applied to the lint based on CCC loan schedule. 6 Value of lint per acre based on CCC loan schedule of discounts and premiums and assuming a base value of 52.00 cents per pound. 2 Arizona Cotton Report (P-138) May 2004 108 Value6 ($/acre) 952 877 920 864 886 863 844 841 725 766 757 721 120000 16 May 3 June S eedlings Per Acre 110000 100000 90000 80000 70000 60000 601 9B R 5B R 90R 5B R 8B R 1B R 91R R IATA L COT5303R599B R AG3 DP 44 D P 55 DP 56 DP 65 F M89 F M99 F M9 S A S T S T5 Figure 1. Number of seedling per acre on two separate sample dates counted for each of the twelve varieties entered into the Graham County Upland Variety Test, 2003. 100 Es timated P ercent Emergence 16 May 3 June 90 80 70 60 50 40 601 9B R 5B R 90R 5B R 8B R 1B R 91R R IATA L COT 5303R599B R AG3 D P 44 D P 55 D P 56 DP 65 F M89 F M99 F M9 S A S T S T5 Figure 2. Estimated percent emergence on two sample dates for each of the twelve varieties entered into the Graham County Upland Variety Test, 2003. Arizona Cotton Report (P-138) May 2004 109 2.5 Height (in.)/Node Ratio 2.0 1.5 AG 3601 DP449BR DP555BR DP5690R DP655BR FM989BR FM991BR FM991R RIATA SCX_7 ST5303R ST5599BR 1.0 0.5 0.0 500 1000 1500 2000 2500 3000 3500 Heat Units Accumulated After P lanting Figure 3. Height (in.) to node ratio trends for each of the 12 varieties planted in the Graham County Upland Variety Test, 2003. 120 P ercent Fruit Retention 100 80 AG 3601 DP449BR DP555BR DP5690R DP655BR FM989BR FM991BR FM991R RIATA SCX_7 ST5303R ST5599BR 60 40 20 0 500 1000 1500 2000 2500 3000 3500 Heat Units Accumulated After P lanting Figure 4. Fruit retention levels for each of the 12 varieties planted in the Graham County Upland Cotton Variety Test, 2003. Arizona Cotton Report (P-138) May 2004 110 Nodes Above Top 1s t P os ition Fres h B loom 16 14 12 10 AG 3601 DP449BR DP555BR DP5690R DP655BR FM989BR FM991BR FM991R RIATA SCX_7 ST5303R ST5599BR 8 6 4 2 0 500 1000 1500 2000 2500 3000 3500 Heat Units Accumulated After P lanting Figure 5. Number of nodes above top, first position fresh bloom for each of the 12 varieties planted in the Graham County Upland Cotton Variety Test, 2003. 1800 950 1600 1500 1400 1300 900 850 800 Value ($/acre) Lint Yield (lbs./acre) 1700 1000 1200 1100 1000 1 A R R R R R R R R R -7 91B 5303 55B 991 5690 55B S C X P449BAG 3605599B RIATM 989B D F F M 9 S T D P6 F M DP DP5 ST 750 700 Figure 6. Lint yield estimates and crop value for each of the twelve varieties entered into the Graham County Upland Variety Test, 2003. Arizona Cotton Report (P-138) May 2004 111 Percent Relative Yield (DP655BR) 105 100 95 90 85 80 75 1 A R R R R R R R R R -7 91B 5303 55B 991 5690 55B SC X P449BAG 3605599B RIATM989B D F M9 S T DP6 F M D P D P5 F ST Figure 7. Percent relative yield (relative to DP655BR) for each of the twelve varieties entered into the Graham County Upland Variety Test, 2003. Percent Relative Value (DP655BR) 105 100 95 90 85 80 75 1 A R R R R R R R R R -7 91B 5303 55B 991 5690 55B SC X P449BAG 3605599B R IATM989B D F M9 S T DP6 F M DP DP5 F ST Figure 8. Percent relative value (relative to DP655BR) for each of the twelve varieties entered into the Graham County Upland Variety Test, 2003. Arizona Cotton Report (P-138) May 2004 112 Premium/Discount (points) 500 400 300 200 100 0 -100 1 A R R R R R R R R R -7 91B 5303 55B 991 5690 55B SC X P449BAG 3605599B RIATM989B D F M9 ST D P6 F M D P D P5 F ST Figure 9. Premium/discount for each of the twelve varieties entered into the Graham County Upland Variety Test, 2003 calculated based upon fiber quality data. 5.2 5.1 Discount Range 5.0 Fiber Micronaire 4.9 4.8 4.7 4.6 4.5 4.4 4.3 4.2 4.1 4.0 1 A R R R R R R R R R -7 91B 5303 55B 991 5690 55B SC X P449BAG 3605599B RIATM 989B D F M 9 S T D P6 F M D P D P5 F ST Figure 10. Fiber micronaire values for each of the twelve varieties entered into the Graham County Upland Variety Test, 2003. Arizona Cotton Report (P-138) May 2004 113 36.0 Staple (32nds of an inch) 35.5 Premium Range 35.0 34.5 34.0 Discount Range 33.5 33.0 32.5 32.0 1 A R R R R R R R R R -7 91B 5303 55B 991 5690 55B SC X P449BAG 3605599B RIATM989B D F M 9 ST D P6 F M D P D P5 F ST Figure 11. Staple length values for each of the twelve varieties entered into the Graham County Upland Variety Test, 2003. 33.0 32.5 Premium Range Fiber Strength (g/tex) 32.0 31.5 31.0 30.5 30.0 29.5 29.0 28.5 28.0 1 A R R R R R R R R R -7 91B 5303 55B 991 5690 55B SC X P449BAG 3605599B RIATM 989B D F M 9 ST D P6 F M D P D P5 F ST Figure 12. Fiber strength values for each of the twelve varieties entered into the Graham County Upland Variety Test, 2003. Arizona Cotton Report (P-138) May 2004 114