Environmental Factors Influencing Wood Frog (Lithobates sylvaticus) Tadpole Size in a Glaciated Landscape Amanda Smith, Dr. James Church and Dr. Mandy Little University of Wisconsin-Stout Introduction Results L. sylvaticus populations are a significant contributor in maintaining a balanced food chain; therefore L. sylvaticus is important to maintain a functional wetland. pH was the only environmental variable to be positively correlated with SVL (Figure 1). Out of the 40 ephemeral ponds surveyed, only half collected L.sylvaticus tadpoles. The mean SVL was 13.7 mm (± 8.19). Minimum SVL was 8.33 mm and maximum was 20.2 mm. Isolated ephemeral wetlands are a significant habitat for amphibians in the forested landscape. Is there an environmental characteristic of wetlands correlated with L. sylvaticus tadpole body size? Objective: evaluate environmental variables as it relates to the assessment of tapole body size. Environmental variables: temperature, pH, and dissolved, chlorophyll-a and canopy cover Figure 1. Correlation of pH and SVL. Each dot represents an ephemeral pond that had L. sylvaticus tadpoles during the time of surveying. The figure indicates a strong positive correlation between pH and SVL. Discussion Methods Chippewa Moraine State Recreation Area 40 ephemeral ponds One sample period in May 2013 Four minnow traps per pond 24 incubation period Snout-vent length (SVL) pH was the only significant factor having a strong correlation with SVL. We believe factors affecting pH are most likely litter inputs in addition to geological wetland characteristics. Studies examining types of leaf litter have indicated that tadpole size may be determined by both the species of amphibian and species of tree (Stoler and Reylea, 2011, p.867). The pH of wetlands can serve as a proxy for the relationship between immediate upland landscapes, most often acidifying Pinaceae family, and the SVL of L. sylvaticus. As pH increases, SVL increases; thus more basic wetlands are more likely to have sustainable populations due to larger amphibians. While our study focuses on L. sylvaticus, it is important to realize the potential implications of our results on other wetland species such as aquatic macro-invertebrates that are essential contributors to water quality. Future studies designed to evaluate multiple species of animals and plants of wetlands would promote a better understanding of biodiversity functions of wetland ecosystems. With expansive research we believe conservation policy could be greatly beneficial to the greater watersheds. Literature Cited Stoler, A.B. and Relyea, R.A. (2011). Living in the litter: the influence of leaf litter on wetland communities. Oikos, 120, 862-867. Tadpole Photo: Photo by Rick Relyea, http://www.myscience.us/wire/stressed_out_tadpoles_grow_larger_tails_to_es cape_predators-2013-umich “Stressed-out tadpoles grow larger tails to escape predators” WI map: http://www.mappery.com/Wisconsin-Counties-Map