USDA ARS GEM Field Day 2003 GEM MINI-NURSERY BOOK GERMPLASM ENHANCEMENT OF MAIZE Central Iowa Field Day DEMONSTRATION PLOT MAP N ENTRY NUMBERS RUN FROM EAST TO WEST 101 150 RANGE 3 POPGEM Populations and Lines / Hybrids from GEM NC State 51 100 RANGE 2 Breeding for Value Added Traits and Experimental Hybrids 1 50 RANGE 1 Breeding History Demonstration and Lines for Potential Release Field History: Fertilizer: Herbicide: Insecticide: Planted on May 20th, 2003. N/P/K 177-0-0. A Dual II Magnum/A Callisto Lorsban GEM homepage: www.public.iastate.edu/~usda-gem Page 1 of 11 USDA ARS GEM Field Day 2003 Germplasm Enhancement of Maize Project USDA-ARS Field day in conjunction with the CAD Field Day at the Bruner Farm September 17 – 18, 2003 The Germplasm Enhancement of Maize (GEM) Project is a cooperative effort of the United States Department of Agriculture’s Agricultural Research Service (USDA-ARS), land-grant universities, private industry, international, and non-governmental organizations. The mission of the project is to broaden the genetic base of maize germplasm, increasing its diversity through introgression of unique and novel germplasm into elite, adapted lines. The GEM Project is administered through the USDAARS’s Plant Introduction Research unit in Ames, IA. Breeding History (rows 1-20): The history of corn breeding usually begins with the original Stiff Stalk Synthetic. Lines derived from this synthetic or advanced cycles were very popular commercially and are important breeding sources designated as stiff stalk (SS) related. A popular non-stiff stalk (NS) family includes Lancaster, and Mo17 and Oh43 are representatives of this group. The popular hybrid B73xMo17 (mid-1970’s-1980’s), is shown adjacent to a modern medium season hybrid. The tropical accession, CUBA164, has been an important source of VAT’s and yield enhancement for GEM. GEM lines for potential release and topcross hybrids (rows 21-50; 71-100): GEM inbred lines (S3 bulks) are planted in range 1 with the adjacent range 2 planted to the top cross counterpart for comparative observation. The tester lines used by GEM are elite Corn Belt inbreds LH185, LH283, LH198, and LH200 (Holden’s Foundation Seed, L.L.C.). The pedigrees of the S3’s are labeled to denote percentage of temperate or tropical exotic germplasm (25% or 50%) in the line, and the country and race of origin. Temperate germplasm in the demo includes Argentina, Chile, Uruguay, USA; tropical germplasm includes Brazil, Cuba, Mexico, St. Croix, and Thailand. Hybrids planted in the demo are in second year trials in 2003, or will be tested for the second year in 2004. Value-added trait (VAT) lines and research (rows 51-70): Grain quality analysis is done in our labs in Ames managed by Sue Duvick. Exotic germplasm is an important source of VAT’s. GEM is studying the composition and quality of protein, oil, and starch in inbred lines and hybrids. Target values include protein content 13%, oil 6%, and starch 75%. Amino acid content is evaluated for lysine, tryptophan, and methionine; and fatty acid analysis is made for oil quality. Starch thermal properties are analyzed to determine predictive functionality for food and industrial applications. Some of the thermal traits being researched include peak temperature onset for starch gelatinization, range of gelatinization, enthalpy and percent retrogradation, and peak height index of thermogram. Inbred lines in the demo are some of the GEM lines developed in Ames, Raleigh, and UDEL that are being further inbred, topcrossed, and/or recombined with other lines for further enhancement of protein, oil, and starch quality. POPGEM (rows 101-112): The POPGEM project started in 2000 and is being coordinated by Ken Ziegler at ISU. The 12 rows are 4 rows each of the 3 heterotic groups in popcorn: AP=Amber Pearl, SA=South American and SG= Supergold. These rows represent the first populations of POPGEM where private company popcorn inbred lines were crossed to exotic material from the Iowa State University and University GEM homepage: www.public.iastate.edu/~usda-gem Page 2 of 11 USDA ARS GEM Field Day 2003 of Missouri popcorn breeding programs. A second set of exotic material from the Iowa State University popcorn breeding program will be crossed to private company popcorn inbred lines next year. GEM material is undergoing popcorn pre-breeding in the ISU popcorn breeding program to prepare it to be the third set of exotic material crossed to popcorn. Seventeen NS and 51 SS GEM populations are being used. NC Raleigh GEM (rows 113-138): GEM’s effort in the southeast is coordinated by USDA-ARS technician Joe Hudyncia, who works closely with Professor Major Goodman at NC State. Hybrids in the demo are being evaluated for adaptability and yield performance throughout the Midwest this year. Several of the lines have unique VAT’s including high protein, oil, and starch thermal properties. GEM Breeding crosses (rows 139-150): New breeding crosses being worked in the GEM nursery in 2003 include eight temperate (25% or 50%) populations, and four 25% tropical populations. Half of the populations are classified as SS, and half are the NS group. S1 families from each breeding cross will be planted and selected next summer in the Midwest. More information on the structure and organization of the GEM Project, yield trial results, GEM accomplishments, and summaries of University and other USDA-ARS research with GEM germplasm can be found on our web site, http://www.public.iastate.edu/~usda-gem/. GEM homepage: www.public.iastate.edu/~usda-gem Page 3 of 11 USDA ARS GEM Entry 1 1 2 2 3 3 4 4 5 5 6 6 7 7 8 8 9 9 10 10 11 11 12 12 13 13 14 14 15 15 16 16 17 17 18 18 19 19 20 20 Field Day 2003 RANGE 1 – Breeding History Demonstration & Lines for Potential Release Pedigree Mid Pollen Comments Breeding History Demonstration Original Stiff Stalk July 27 B14 July 29 B37 July 29 B64 August 3 B68 August 2 B73 July 30 B84 July 31 Lancaster Sure Crop < July 21 Mo17 August 1 Oh43 July 26 B73 x Mo17 July 26 Modern Commercial Hybrid (medium season) July 24 CUBA164 Aug. 25-30 CUBA164:S20 August 1 CUBA164:S2012 July 31 CUBA164:S2012-444-1-B August 3 CUBA164:S2012-444-1/LH185 July 27 Modern Commercial Hybrid (full season) July 31 CUBA164:S2012-444-1-B/LH283 July 31 AR16026:S1704-139-1/LH185 July 26 21 AR16026:S1704-153-1-B 21 30 GEM Lines for Potential Release July 31 GEM homepage: www.public.iastate.edu/~usda-gem Page 4 of 11 USDA ARS GEM 22 22 23 23 24 24 25 25 26 26 27 27 28 28 29 29 30 30 31 31 32 32 33 33 34 34 35 35 36 36 37 37 38 38 39 39 40 40 41 41 42 42 43 43 44 44 Field Day 2003 AR16035:S02-450-1-B-B August 3 AR16035:S02-611-1-B-B August 1 AR16035:S02-615-1-B-B August 2 AR16035:S19-285-1-B August 4 CH05015:N1204-380-1-B July 31 CH05015:N12-183-1-B-B August 3 CH05015:N15-3-1-B-B August 1 CHIS740:S1411a-783-2-B July 30 CHIS775:S1911b-439-1-B July 31 CUBA117:S15-101-1-B-B August 2 CUBA164:S1511b-325-1-B July 31 CUBA164:S1517-163-1-B July 29 CUBA164:S2012-444-1-B August 3 CUBA164:S2012-966-1-B July 27 DK212T:N11a-138-1-B August 3 DKB844:S1601-3-2-B August 4 DKXL212:N11a-139-1-1-B August 2 DKXL370:N11a20-322-B July 31 DKXL370:N11a20-36-2-B July 27 FS8(A)S:S09-349-1-B-B-B August 1 FS8A(S):S09-362-1-B August 2 FS8B(S):S0316-1168-1-B-B-B July 31 FS8B(T):N11a-87-1-B July 29 GEM homepage: www.public.iastate.edu/~usda-gem Page 5 of 11 USDA ARS GEM 45 45 46 46 47 47 48 48 49 49 50 50 FS8B(T):N1809-946-1-B-B Field Day 2003 August 2 SCR01:N1310-358-1-B-B July 28 SCR01:N1310-509-1-B-B August 4 UR01089:S0525-519-1-B July 29 UR10001:S1813-257-1 UR13085:N0215-14-1-B August 2 July 28 RANGE 2 – Breeding for Value-Added Traits & Experimental Hybrids Pedigree Mid Pollen Comments Breeding for Value-Added Traits * 51 DK212T:S11 F2S4 2089-01 August 3 51 52 SE32(BR052051):S17 F2S4 2011-01 August 2 52 53 DKB830:S11 F2S4 2201-01 August 3 53 54 AR17056:N2025-574-1-B-B July 29 54 55 CUBA164:S1511b-325-1-B August 3 55 56 DKB844:S1601-491-1-B August 5 56 57 CUBA164:S15-118-6-9-B-B August 4 57 58 CUBA164:S1517-241-2-B August 1 58 59 CUBA164:S2012-459-1-B July 29 59 60 FS8B(S):S0316-53-2-B August 2 60 61 DXKL370:N11a20-410-1-B July 28 61 62 CUBA164:S15-118-6-13-B-B August 4 62 63 UR01089:S0525-3-1-B August 1 63 64 FS8B(S):S0316-230-2-B August 2 64 65 AR16026:S17-104-1-B August 1 65 Entry GEM homepage: www.public.iastate.edu/~usda-gem Page 6 of 11 USDA ARS GEM Field Day 2003 66 66 67 67 68 68 69 69 70 70 AR16026:S17-211-1-B July 29 DE4 (UDEL) July 31 DE3 (UDEL) July 29 71 71 72 72 73 73 74 74 75 75 76 76 77 77 78 78 79 79 80 80 81 81 82 82 83 83 84 84 85 85 86 86 87 87 88 88 AR16026:S1704-153-1/LH283 DKXL212:N11a-234-1-1-1-1-1-1-1-1 August 3 PE001n16F2S2-44 August 5 Experimental Hybrids – Topcrosses of the Lines in Range 1 July 29 AR16035:S02-450-1-B-B/LH185 July 26 AR16035:S02-611-1-B-B/LH185 July 26 AR16035:S02-615-1-B-B/LH185 July 26 AR16035:S19-285-1-B/LH185 July 26 CH05015:N1204-380-1/LH198 July 25 CH05015:N12-183-1-B/LH198 July 29 CH05015:N15-3-1-B/LH198 July 25 CHIS740:S1411a-783-2/LH185 July 26 CHIS775:S1911b-439-1/LH185 July 25 CUBA117:S15-101-1-B/LH185 July 26 CUBA164:S1511b-325-1/LH185 July 26 CUBA164:S1517-163-1/LH185 July 26 CUBA164:S2012-444-1/LH185 July 27 CUBA164:S2012-966-1/LH185 July 25 DK212T:N11a-138-1/LH198 July 29 DK844:S1601-3-2/LH185 July 27 DKXL212:N11a-139-1-1/LH198 July 29 GEM homepage: www.public.iastate.edu/~usda-gem Page 7 of 11 USDA ARS GEM 89 89 90 90 91 91 92 92 93 93 94 94 95 95 96 96 97 97 98 98 99 99 100 100 Field Day 2003 DKXL370:N11a20-322/LH198 July 26 DKXL370:N11a20-36-2-B-B/LH198 July 27 FS8(A)S:S09-349-1-B-B/LH283 July 25 FS8(A)S:S09-362-1-B-B/LH185 July 25 FS8B(S):S0316-1168-1-B-B/LH185 July 27 FS8B(T):N11a-87-1/LH198 July 27 FS8B(T):N1809-946-1-B-B/LH198 July 28 SCR01:N1310-378-1-B-B/LH198 July 29 SCR01:N1310-509-1-B-B/LH198 July 27 UR01089:S0525-519-1/LH283 July 29 UR10001:S1813-257-1/LH185 July 26 UR13085:N0215-14-1-B/LH198 July 26 RANGE 3 – POPGEM Populations and Hybrids from GEM NC State Entry Pedigree Mid Pollen Comments Experimental Populations from POPGEM 101 POPGEM AP --F2 bulk of Flints and Dents crossed to 101 Amber Pearl type Popcorns. 102 POPGEM AP --102 103 POPGEM AP --103 104 POPGEM AP --104 105 POPGEM SA --F2 bulk of Flints and Dents crossed to 105 South American type Popcorns. 106 POPGEM SA --106 107 POPGEM SA --107 108 POPGEM SA --108 109 POPGEM SG --F2 bulk of Flints and Dents crossed to 109 Supergold type Popcorns. GEM homepage: www.public.iastate.edu/~usda-gem Page 8 of 11 USDA ARS GEM 110 110 111 111 112 112 Field Day 2003 POPGEM SG --- POPGEM SG --- POPGEM SG --- Hybrids from GEM NC State Raleigh 113 DK370A:S11 F2S4 2253-01/LH185 July 26 113 114 DK888:S11 F2S4 2131-01/LH185 July 25 114 115 DK212T:S11 F2S4 2109-01/LH283 July 26 115 116 DK212T:S11 F2S4 2111-01/LH185 July 27 116 117 DK212T:S11 F2S4 2112-02/LH185 July 26 117 118 DK888:S11 F2S4 2127-01/LH283 July 31 118 119 DK888:S11 F2S4 2132-03/LH185 July 25 119 120 DK212T:S11 F2S4 2086-01/LH185 July 29 120 121 DK888:S11 F2S4 2143-02/LH185 July 28 121 122 DK370A:S11 F2S4 2250-02/LH185 July 29 122 123 DK888:S11 F2S4 2150-01/LH283 July 31 123 124 DK888:S11 F2S4 2152-03/LH185 July 27 124 125 DKB830:S11 F2S4 2201-01/LH283 Aug. 1 125 126 PE001n16F2S2-172/LH200 Aug. 1 126 127 PE001n16F2S2-176/LH198 Aug. 1 127 128 DK888:S11 F2S4 2121-04/LH185 July 27 128 129 PE001n16F2S2-193/LH198 July 31 129 130 PE001n16F2S2-239/LH198 July 30 130 GEM homepage: www.public.iastate.edu/~usda-gem Page 9 of 11 USDA ARS GEM 131 131 132 132 133 133 134 134 135 135 136 136 137 137 138 138 139 139 140 140 141 141 142 142 143 143 144 144 145 145 146 146 147 147 148 148 149 149 150 150 Field Day 2003 PE001n16F2S2-431/LH198 July 29 PE001n16F2S2-44/LH198 August 1 PE001n16F2S2-521/LH198 July 31 PE001n16F2S2-65/LH198 July 31 PE001n16F2S2-705/LH198 August 2 PE001n16F2S2-857/LH198 July 30 SE32(BR052051):S17 F2S4 2011-01/LH185 July 30 XL370A:S11 F2S4 2226-02/LH185 July 31 AR16026:N1210 GEM Breeding Crosses July 27 AR16035:S3115 July 27 AR17056:N20 July 26 BIGWHITE:S0330 July 24 BR051501:S11a20 July 30 DKB830:S11a17 July 26 DKXL212:S0912 July 26 SCROGP3:N2001 July 27 UR01089:N22 July 28 UR10001:S18 July 28 UR11002:N0302 July 26 UR13085:N02 July 26 * For further information, please see the Appendix on the next page. GEM homepage: www.public.iastate.edu/~usda-gem Page 10 of 11 USDA ARS GEM Field Day 2003 APPENDIX Entry Number Pedigree Description DM % Protein DM % Oil DM % Starch 17.0 3.3 66.5 15.0 5.1 66.3 10.1 2.5 71.3 16.6 3.0 67.1 16.6 4.1 63.9 16.2 3.4 66.3 16.0 3.7 66.9 15.9 4.8 64.2 15.7 3.9 66.7 15.4 3.4 66.9 15.2 3.8 67.7 15.1 3.8 68.4 15.0 4.2 67.0 15.0 4.7 67.0 11.3 2.4 71.2 12.4 2.8 71.1 51 DK212T:S11 F2S4 2089-01 NC-GEM 52 SE32(BR52 051):S17 F2S4 2011-01 NC-GEM 53 DKB830:S11 F2S4 2201-01 NC-GEM 54 AR17056:N2025-574-1-B-B Starch quality: high temp gelat; high % retrogradation. 55 CUBA164:S1511b-325-1-B See hybrid entry 82 56 DKB844:S1601-491-1-B Composition research 57 CUBA164:S15-118-6-9-B-B Composition research 58 CUBA164:S1517-241-2-B Composition research 59 CUBA164:S2012-459-1-B Composition research 60 FS8B(S):S0316-53-2-B Starch quality: low temp gelatinization; 61 DXKL370:N11a20-410-1-B Composition research 62 CUBA164:S15-118-6-13-B-B Composition research 63 UR01089:S0525-3-1-B Composition research 64 FS8B(S):S0316-230-2-B Composition research 65 AR16026:S17-104-1-B Composition research 66 AR16026:S17-211-1-B Composition research 4.3 65.7 DE4 GEM derived line from UDEL 14.6 67 3.8 64.3 DE3 GEM derived line from UDEL 16.6 68 DKXL212:N11a-234-1-1-1-11-1-1-1 GEM derived line from UDEL 16.6 4.4 64.0 69 12.0 3.8 68.2 70 PE001n16F2S2-44 NC-GEM GEM homepage: www.public.iastate.edu/~usda-gem Page 11 of 11