USDA ARS GEM
Field Day 2001
GEM MINI-NURSERY BOOK
GERMPLASM ENHANCEMENT OF MAIZE
Central Iowa Field Day
DEMONSTRATION PLOT MAP
N
ENTRY NUMBERS RUN FROM WEST TO EAST
1
50
RANGE 1
Breeding History Demonstration and Some Set B Lines
(2 ROW PLOTS)
51
100
RANGE 2
Breeding for Value-Added Traits and Experimental Hybrids
(2 ROW PLOTS)
Raleigh 101
Raleigh 150
RANGE 3
Experimental Hybrids and Lines from GEM in Raleigh
(2ROW PLOTS)
Field History:
Fertilizer:
Herbicide:
Planted on May 15th, 2001.
N/P/K 150-80-110.
PPI, 30 oz./A Frontier.
2 pt/A Laddock post application.
GEM homepage: www.public.iastate.edu/~usda-gem
Page 1 of 12
USDA ARS GEM
Field Day 2001
RANGE 1 - BREEDING HISTORY DEMOSTRATION & SET B LINES FOR POTENTIAL RELEASE
Entry
Pedigree
Comments
BREEDING HISTORY DEMONSTRATION
1 Reid Yellow Dent (Open Pollinated Population)
1
2 Original Stiff Stalk Synthetic
2
3 Inbred Line B73
3
4 Public Single Cross B73 x Mo17
4
5 Commercial Hybrid
5
6 CUBA 164
6
7 CUBA164:S20
7
8 CUBA164:S2008a
8
9 CUBA164:S2008a-6-1-B-B
9
10 CUBA164:S2008a-6-1-B x LH185
10
Lancaster Sure Crop (Open Pollinated
11 Population)
11
12 BS26 (Lancaster Composite)
12
13 Inbred Line Mo17
13
14 Public Single Cross B73 x Mo17
14
15 Commercial Hybrid
15
16 CHZM 05 015
16
17 CH05015:N12
17
18 CH05015:N1204
18
19 CH05015:N12-18-1-B
19
20 CH05015:N12-18-1-B x LH198
20
29 SET B LINES FOR POTENTIAL RELEASE (WE HAVE 60 TOTAL)
21 CUBA164:S15-184-1-B
21
GEM homepage: www.public.iastate.edu/~usda-gem
Page 2 of 12
USDA ARS GEM
22
22
23
23
24
24
25
25
26
26
27
27
28
28
29
29
30
30
31
31
32
32
33
33
34
34
35
35
36
36
37
37
38
38
39
39
40
40
41
41
42
42
43
43
44
44
Field Day 2001
CUBA164:S15-192-1-B
CUBA164:S15-253-1-B
CUBA164:S15-337-1-B
CUBA164:S15-435-1-B
CUBA164:S2008a-157-1-B
CUBA164:S2008a-219-1-B
CUBA164:S2008a-280-1-B
CUBA164:S2008a-326-1-B
CUBA164:S2008a-469-1-B
DKB844:S1601-507-1-B
DKB844:S1601-512-1-B
AR16035:S02-450-1-B
AR16035:S02-611-1-B
AR16035:S02-615-1-B
AR16035:S02-672-1-B
AR16035:S02-674-1-B
SCRO1:N1310-78-1-B
SCRO1:N1310-353-1-B
SCRO1:N1310-358-1-B
SCRO1:N1310-378-1-B
SCRO1:N1310-509-1-B
CHIS775:N1912-519-1-B
Planting check - purple plant/yellow seed
GEM homepage: www.public.iastate.edu/~usda-gem
Page 3 of 12
USDA ARS GEM
45
45
46
46
47
47
48
48
49
49
50
50
Field Day 2001
AR01150:N04-133-1-B
AR01150:N04-545-1-B
AR01150:N04-692-1-B
FS8B(T):N1809-843-1-B
FS8B(T):N1809-851-1-B
FS8B(T):N1809-946-1-B
RANGE 2 - BREEDING FOR VALUE-ADDED TRAITS & EXPERIMENTAL HYBRIDS
Pedigree
Comments
BREEDING FOR VALUE-ADDED TRAITS *
51 Blank
51
52 Blank
52
Breeding Program for Amylose Content (Data from Mark Campbell, Truman State University)
53 [GUAT209:S13 x (OH43ae x H99ae)]-222-1-1 Amylose Content 46.6% vs. mean of 53.3%
53
54 [GUAT209:S13 x (OH43ae x H99ae)]-219-1-1 Amylose Content 62.2% vs. mean of 53.3%
54
55 [GUAT209:S13 x (OH43ae x H99ae)]-233-1-1 Amylose Content 68.0% vs. mean of 53.3%
55
[GUAT209:S13 x (OH43ae x H99ae)]-268-1-1
56 x B73 ae type tester
Amylose Content 73.6% vs. mean of 53.3%
56
[GUAT209:S13 x (OH43ae x H99ae)]-275-1-1
57 x B73 ae type tester
Amylose Content 62.3% vs. mean of 53.3%
57
[GUAT209:S13 x (OH43ae x H99ae)]-273-1-1
58 x B73 ae type tester
Amylose Content 72.5% vs. mean of 53.3%
58
Wet Milling Evaluations (Data from Cerestar)
59 CUBA164:S15-184-1-B
Wet Milling Character: Low % extractable starch, 39.99%
59
% Starch of B73 x Mo17: 61.1%
60 CUBA164:S2008a-3-1-B
Wet Milling Character: High % extractable starch, 62.69%
60
61 DKB844:S1601-512-1-B
Wet Milling Character: High % gluten, 17.31%
61
% Gluten of B73 x Mo17: 11.1%
Breeding Program for Composition (Data from Linda Pollak, USDA-ARS)
62 CH05015:N12-43-1-B-B
High Oil: 5.38% oil, 12.64 % protein, and 67.36% starch
62
Oil % of B73, 3.4%; Mo17, 3.3%; and B73 x Mo17, 3.8%
Entry
GEM homepage: www.public.iastate.edu/~usda-gem
Page 4 of 12
USDA ARS GEM
63 CH05015:N12-50-1-B-B
63
64 AR16035:S02-235-1-1-6-6-11-B
Field Day 2001
High Starch: 70.98% starch, 11.35% protein, and 3.25% oil
Starch % of B73, 71.8%; Mo17 71.6%; and B73 x Mo17,
70.4%
High Protein: 17.4% protein, 4.6% oil, and 65.0% starch
Protein % of B73, 11.9%; Mo17 11.9%, and B73 x Mo17,
12.9%
Low Protein: 9.8% protein, 4.1% oil, and 67.0% starch
64
65 AR16035:S02-235-1-1-6-4-16-B
65
66 SCRO1:N1310-338-31-6-2-1-6-B
High Oil: 7.0% oil, 12.0% protein, and 66.5% starch
66
67 SCRO1:N1310-338-31-6-2-1-5-B
Low Oil: 3.4% oil, 11.1% protein, and 71.0% starch
67
Breeding Program for Silage Quality (Data from Jim Coors, University of Wisconsin)
68 DKXL212:N11a-2-1-1 **
DKXL212:N11a-2-1 x LH198 had 9.35 tons/acre forage yield
68
69 CUBA117:S1520-156-1-B **
CUBA117:S1520-156-1 x LH185 had
69
6.65 tons/acre forage yield with 31.4% dry matter
Breeding Program for Low Grain Phosphorus (Data from Ken Russell, University of Nebraska)
70 CHIS740:S1411a
Low Grain Phosphorus: 0.211%
70
EXPERIMENTAL HYBRIDS - TOPCROSSES OF THE SET B LINES FOR RELEASE IN RANGE 1
71 CUBA164:S15-184-1-B x LH185
71
72 CUBA164:S15-192-1-B x LH185
72
73 CUBA164:S15-253-1-B x LH185
73
74 CUBA164:S15-337-1-B x LH185
74
75 CUBA164:S15-435-1-B x LH185
75
76 CUBA164:S2008a-157-1-B x LH185
76
77 CUBA164:S2008a-219-1-B x LH185
77
78 CUBA164:S2008a-280-1-B x LH185
78
79 CUBA164:S2008a-326-1-B x LH185
79
80 CUBA164:S2008a-469-1-B x LH185
80
81 DKB844:S1601-507-1-B x LH185
81
82 DKB844:S1601-512-1-B x LH185
82
83 AR16035:S02-450-1-B x LH185
83
GEM homepage: www.public.iastate.edu/~usda-gem
Page 5 of 12
USDA ARS GEM
84
84
85
85
86
86
87
87
88
88
89
89
90
90
91
91
92
92
93
93
94
94
95
95
96
96
97
97
98
98
99
99
100
100
Field Day 2001
AR16035:S02-611-1-B x LH185
AR16035:S02-615-1-B x LH185
AR16035:S02-672-1-B x LH185
AR16035:S02-674-1-B x LH185
SCRO1:N1310-78-1-B x LH198
SCRO1:N1310-353-1-B x LH198
SCRO1:N1310-358-1-B x LH198
SCRO1:N1310-378-1-B x LH198
SCRO1:N1310-509-1-B x LH198
CHIS775:N1912-519-1-B x LH198
CHIS775:N1912-651-1-B x LH198
AR01150:N04-133-1-B x LH198
AR01150:N04-545-1-B x LH198
AR01150:N04-692-1-B x LH198
FS8B(T):N1809-843-1-B x LH198
FS8B(T):N1809-851-1-B x LH198
FS8B(T):N1809-946-1-B x LH198
RANGE 3 – EXPERIMENTAL HYBRIDS AND LINES FROM GEM IN RALEIGH
Entry
Pedigree
Comments
(BR052051:S17 F2S3 2011-01) x (FR615 x
101 FR697)
101
(DK212T:S11 F2S3 2084-02) x (FR615 x
102 FR697)
102
(DK212T:S11 F2S3 2086-01) x (FR615 x
103 FR697)
103
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Page 6 of 12
USDA ARS GEM
Field Day 2001
(DK212T:S11 F2S3 2088-01) x (FR615 x
104 FR697)
104
(DK212T:S11 F2S3 2089-01) x (FR615 x
105 FR697)
105
(DK212T:S11 F2S3 2109-01) x (FR615 x
106 FR697)
106
(DK212T:S11 F2S3 2111-01) x (FR615 x
107 FR697)
107
(DK212T:S11 F2S3 2112-02) x (FR615 x
108 FR697)
108
(DK212T:S11 F2S3 2116-02) x (FR615 x
109 FR697)
109
110 (DK888:S11 F2S3 2120-01) x (FR615 x FR697)
110
111 (DK888:S11 F2S3 2121-04) x (FR615 x FR697)
111
112 (DK888:S11 F2S3 2127-01) x (FR615 x FR697)
112
113 (DK888:S11 F2S3 2131-01) x (FR615 x FR697)
113
114 (DK888:S11 F2S3 2132-03) x (FR615 x FR697)
114
115 (DK888:S11 F2S3 2142-01) x (FR615 x FR697)
115
116 (DK888:S11 F2S3 2143-02) x (FR615 x FR697)
116
117 (DK888:S11 F2S3 2146-01) x (FR615 x FR697)
117
118 (DK888:S11 F2S3 2150-01) x (FR615 x FR697)
118
119 (DK888:S11 F2S3 2152-02) x (FR615 x FR697)
119
120 (DK888:S11 F2S3 2152-03) x (FR615 x FR697)
120
121 (DK888:S11 F2S3 2156-02) x (FR615 x FR697)
121
(DKB830:S11 F2S3 2201-01) x (FR615 x
122 FR697)
122
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Page 7 of 12
USDA ARS GEM
Field Day 2001
(DKXL370A:S11 F2S3 2226-02) x (FR615 x
123 FR697)
123
(DKXL370A:S11 F2S3 2228-03) x (FR615 x
124 FR697)
124
(DKXL370A:S11 F2S3 2250-01) x (FR615 x
125 FR697)
125
(DKXL370A:S11 F2S3 2250-02) x (FR615 x
126 FR697)
126
(DKXL370A:S11 F2S3 2253-01) x (FR615 x
127 FR697)
127
(DKXL380:S11 F2S3 2258-03) x (FR615 x
128 FR697)
128
(DKXL380:S11 F2S3 2282-01) x (FR615 x
129 FR697)
129
(DKXL380:S11 F2S3 2283-01) x (FR615 x
130 FR697)
130
131 (BR51403:N16 F2S3 44) x (FR992 x FR1064)
131
132 (BR51403:N16 F2S3 181) x (FR992 x FR1064)
132
133 (BR51403:N16 F2S3 239) x (FR992 x FR1064)
133
134 (BR51403:N16 F2S3 705) x (FR992 x FR1064)
134
135 Pioneer 3165
135
136 Pioneer 32K61
136
137 LH132 x LH51
137
138 DeKalb DK687
138
139 BR51403:N16 F2S2 44
139
140 BR51403:N16 F2S2 65
140
141 BR51403:N16 F2S2 173
141
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Page 8 of 12
USDA ARS GEM
142
142
143
143
144
144
145
145
146
146
147
147
148
148
149
149
150
150
Field Day 2001
BR51403:N16 F2S2 176
BR51403:N16 F2S2 181
BR51403:N16 F2S2 193
BR51403:N16 F2S2 239
BR51403:N16 F2S2 341
BR51403:N16 F2S2 431
BR51403:N16 F2S2 521
BR51403:N16 F2S2 705
BR51403:N16 F2S2 857
* For further information, please see the Appendix on the next page.
** These lines are more advanced versions of those lines shown in the data.
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Page 9 of 12
USDA ARS GEM
Field Day 2001
APPENDIX
Entries 53 to 58:
Results from a breeding program for high amylose content (Data from Mark Campbell,
Truman State University)
Results from screening S3 lines
[GUAT209:S13 x (OH43ae x H99ae)]-222-1-1
[GUAT209:S13 x (OH43ae x H99ae)]-219-1-1
[GUAT209:S13 x (OH43ae x H99ae)]-233-1-1
Results from screening hybrids of S3 lines with commercial tester:
[GUAT209:S13 x (OH43ae x H99ae)]-268-1-1 x B73 ae type tester
[GUAT209:S13 x (OH43ae x H99ae)]-275-1-1 x B73 ae type tester
[GUAT209:S13 x (OH43ae x H99ae)]-273-1-1 x B73 ae type tester
Starch apparent amylose (aa) values determined from individual ears and heat units to pollination for S3 lines
previously selected for high-amylose content from GEM crosses.
2000
HU
nursery
aa
aa
aa
to
row
Pedigree
ear 1
ear 2
mean pollination
%
%
%
HU
268
[GUAT209:S13 x (OH43ae x H99ae)]62.4
30.8
46.6
1672
268-1-1 x B73 ae type tester
273
[GUAT209:S13 x (OH43ae x H99ae)]65.7
58.7
62.2
1581
273-1-1 x B73 ae type tester
275
[GUAT209:S13 x (OH43ae x H99ae)]67.0
69.0
68.0
1624
275-1-1 x B73 ae type tester
219
[GUAT209:S13 x (OH43ae x H99ae)]75.1
72.2
73.6
1600
219-1-1
222
[GUAT209:S13 x (OH43ae x H99ae)]67.9
56.7
62.3
1624
222-1-1
233
[GUAT209:S13 x (OH43ae x H99ae)]74.5
70.4
72.5
1479
233-1-1
322
B73 check
52.7
55.1
53.9
323
B73 check
54.8
50.3
52.6
Mean
LSD
63.7
61.6
62.6
12.5
Entries 59 to 61:
Wet Milling (Data from Cerestar)
Degree of variation for starch extractability in a laboratory-scale screening of S3 lines:
CUBA164:S15-184-1-B 39.99 low % extractable starch
CUBA164:S2008a-3-1-B 62.69 high% extractable starch
GEM homepage: www.public.iastate.edu/~usda-gem
Page 10 of 12
USDA ARS GEM
Field Day 2001
Result from laboratory-scale screening of S3 lines:
DKB844:S1601-512-1-B 17.31 high % gluten
SAMPLE
SOURCE
%Steep %Fine %Coarse Total
Water Fiber Fiber
Fiber
%
%
%
Total
Germ Starch Gluten Yield
Cuba164:S15-184-1-B
Cuba164:S2008a-3-1-B
DKB844:S1601-512-1-B
B73 x Mo17
Hybrid Control 1
Hybrid Control 2
Hybrid Control 3
I99N:2042-2046
I99N:2132-2136
I99N:2142-2146
I98:Atomic Fence
4.23
3.61
3.61
3.53
3.61
3.80
3.79
5.13
1.44
4.86
7.56
6.06
6.10
6.57
30.52
11.36
14.86
11.24
9.16
9.44
13.18
5.26
6.29
8.48
5.10
6.99
6.34
5.61
35.78
17.66
23.34
16.34
16.15
15.78
18.79
39.99
62.69
50.64
61.06
63.37
63.96
59.41
13.31
14.36
17.31
11.09
9.60
9.00
10.28
98.44
99.77
99.76
99.59
98.79
98.63
98.84
Entries 62 to 67:
Composition (Data from Linda Pollak, USDA-ARS)
Results from screening S3 lines:
CH05015:N12-43-1-B-B protein 12.64 oil 5.38 starch 67.36 over 3 locations, high oil
CH05015:N12-50-1-B-B protein 11.35 oil 3.25 starch 70.98 over 3 locations, high starch
Results from a breeding program:
AR16035:S02-235-1-1-6-6-11-B
AR16035:S02-235-1-1-6-4-16-B
SCRO1:N1310-338-31-6-2-1-6-B
SCRO1:N1310-338-31-6-2-1-5-B
Check Material
B73
Mo17
B73 x Mo17
Pioneer 3394
Pioneer 3489
protein 17.4 oil 4.6 starch 65.0, High Protein
protein 9.8 oil 4.1 starch 67.0, Low Protein
protein 12.0 oil 7.0 starch 66.5, High Oil
protein 11.1 oil 3.4 starch 71.0, Low Oil
Public inbred
Public inbred
Public hybrid
Commercial Hybrid
Commercial Hybrid
Protein
11.9
11.9
12.9
11.4
9.1
Oil
3.4
3.3
3.8
3.3
4.0
Starch
71.8
71.6
70.4
71.5
71.9
Entries 68 to 69:
Silage Quality (Data from Jim Coors, University of Wisconsin)
Selections from an evaluation of source material for a breeding program:
DK212:N11a-2-1-1 DKXL212:N11a-2-1 x LH198 had 9.35 tons/acre forage yield, 23,541 lbs milk/acre
(DKXL212:N11a-2-1 was from Jim Hawk’s breeding program at University of Delaware).
CUBA117:S1520-156-1 CUBA117:S1520-156-1 x LH185 had 2714 lbs milk/ton due to low NDF and high
IVNDF.
GEM homepage: www.public.iastate.edu/~usda-gem
Page 11 of 12
USDA ARS GEM
Field Day 2001
Selected results from a silage evaluation of forage yield and quality of topcrosses of 10 GEM S3 lines and 13
experimental/check hybrids.
Dry
Entry
Matter Yield NDF IVD IVNDFD Protein Starch Milk/ton Milk/acre
%
t/a
%
%
%
%
%
lbs
lbs
DKXL212:N11a-2-1 x LH198
28.0
9.35
57.6
68.7
45.6
6.86
14.5
2501
23541
CUBA117:S1520-156-1 x
LH185
F657 (brown midrib)
P33A14A
GARST 8751
TMF113 (lfy)
NK4687 (lfy)
P35R58
DAIRYLAND STEALTH 1297
31.4
6.65
53.6
71.2
46.3
6.68
23.0
2714
18553
36.6
33.8
52.1
31.3
36.2
39.9
52.9
5.42
8.34
7.65
8.60
7.70
8.45
6.98
52.1
55.3
52.2
59.2
54.3
53.4
50.2
74.7
68.7
70.6
66.7
70.1
70.2
72.5
51.3
43.4
43.7
43.9
45.0
44.3
45.1
7.09
7.01
6.86
6.89
6.58
6.71
6.95
24.8
20.5
25.7
13.8
20.8
24.5
28.0
2926
2434
2417
2326
2533
2492
2511
16150
20398
18713
20344
19714
21457
17591
MEAN
CV (%)
LSD (0.05)
36.50
9.1
8.2
7.48
11.5
0.98
54.4
6.6
5.7
70.1
3.0
3.4
45.2
3.4
1.8
6.81
5.4
0.43
21.8
18.5
4.6
2539
5.5
237
19146
14.6
4106
Entry 70:
Amount of Grain Phosphorus (Data from Ken Russell, University of Nebraska)
Selection from an evaluation of source material for a breeding program (Ken Russell, University of Nebraska):
CHIS740:S1411a (mean=.211%, range 0.166-0.289%) low grain P
This compares to 0.26% for the Stiff-Stalk F2, which had the lowest reading of the 3 Corn Belt F2s. We cannot
say how statistically significant these differences are because we do not have a measurement of error for this
study. However, in an adjacent study where phosphorus concentration was measured among S1 families based
on seed from 3-4 bulked ears from each of 2 replications, the LSD for S1s was 0.08%.
Results from screening S3 lines
GEM homepage: www.public.iastate.edu/~usda-gem
Page 12 of 12