2008 Meeting | Understanding differential virulence within Fusarium vir...
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APS Abstract of Presentation
Understanding differential virulence within Fusarium virguliforme using
multiloci fingerprint analyses
G. C. MBOFUNG (1), T. C. Harrington (1), J. Steimel (1), X. Yang (1), S. S. Navi (1), L. F.
Leandro (1)
(1) Iowa State University
Phytopathology 98:S101
Sudden death syndrome, caused by Fusarium virguliforme, is presently the second most
damaging disease affecting soybean production in the Midwest. Although isolates of the
pathogen have consistently exhibited differing aggressiveness on susceptible soybean
cultivars, DNA sequence analyses of multiple loci have demonstrated a genetically
homogenous population in the USA. To further investigate the genetics behind this
variation in virulence, fingerprint analyses were conducted on isolates originating from
both Iowa and Minnesota. A total of 80 isolates were analyzed targeting four different
loci. Inter-simple sequence repeat primers M13, T3B, (GTG)(5), and (GACA)(5) yielded
identical fingerprint patterns for all isolates. Twelve RAPD primers resolved the isolates
into seven genotypes. The use of (CAT)(5) and (CAC)(5) as fingerprint probes also
delineated the isolates into five genotypes. About 80% of isolates were identical, while
isolates belonging to the other four groups differed depending on the locus under
consideration. The different groups did not correlate well to area of origin.
Representatives of each genotype were tested for aggressiveness on a susceptible
soybean variety. The correlation of aggressiveness on soybean with DNA polymorphisms
based on the different loci will be discussed.
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6/2/2008 2:24 PM