Associated Press 07-21-07 Ames scientist breaks down biofuel research By THE ASSOCIATED PRESS AMES — A laser beam bounces off a series of mirrors before diving into Emily Smith’s microscope, where it scatters as it hits the membrane walls of plant cells, producing a unique array of lightwaves. The waves appear as light and dark crescent patterns on a computer monitor next to the microscope. From them Smith hopes to identify which species of plants contain the optimum raw material for ethanol production. Ethanol manufacturers use corn now, but most experts see it as a transitional feed stock. They believe a plant with more fuel efficiency, possibly switchgrass or wood chips, eventually will replace corn as the chief ingredient in ethanol. For now, breaking down the sugars in such plants remains too expensive compared with using grain. But refineries in Emmetsburg and elsewhere hope to change that in the next few years. Smith, 31, is a research chemist and assistant professor at Iowa State University. She’s beginning a two-year study that could result in dramatic changes in Iowa agriculture. The plant material Smith will study will come from corn plants, as well as a variety of grasses and trees. The more sugar a plant has, the higher the energy potential, she explained. The more lignin, or woody connective tissue, the less energy. The trick is to identify a plant with the optimum combination of the ingredients needed for distilling ethanol. When Smith’s laser passes through sugary cellulose and hemicellulose tissues, the light scatters into patterns that create a fingerprint of sorts for the material. Higher concentrations of lignin result in a much different fingerprint. Optimal ethanol stock ‘‘will have a lot of cellulose and fairly little lignin,’’ Smith said. Before coming to Ames last August, Smith did research at the University of Arizona under a professor who studied how a particular protein interacts to contribute to cancer metastasis. Smith was recruited by ISU to do similar cellular research. She arrived in Ames with the idea of continuing to work with animal cells. ‘‘But after coming here, I realized it was a very wide-open opportunity, and there were so many people on campus with expertise in plants,’’ she said. She talked with agronomy professor Kenneth Moore and experts at ISU’s Plant Sciences Institute on how she might contribute and decided she could help determine which plants or parts of plants are best suited for ethanol production. With her chemistry background, Smith brings a fresh perspective to ethanol research, Moore said. ‘‘It’s really important to have bright, creative people like Emily involved,’’ he said. ‘‘Her tool can help us sort out the structural tissues in plants as they relate to recovering the sugars’’ needed to produce ethanol, Moore said. ‘‘She can prototype the cell wall structure we are looking for, and then we can go out and find that in a plant.’’ The research has the potential to dramatically change the ethanol industry. If Smith’s findings show that a particular type of grass is the best raw material for ethanol production, it could be the catalyst to spur the conversion of millions of acres of farmland from corn to grass production. That in turn would require the creation of new harvesting and handling equipment, new fertilization and cultivating techniques, Moore said. ‘‘It’s going to transfer agriculture in ways we can’t even imagine,’’ he said. But that’s getting a bit ahead of the game. Smith is just beginning her research under a grant to the Ames Laboratory from the U.S. Department of Energy. The first step, she said, is to establish controls that will determine which light patterns mean what in plant cell structures. The controls include translating wave patterns into mathematical formulas, which a computer can use to quickly make comparisons between different plants, or different parts of the same plant. Once the controls are in place, scientists can begin experiments to determine which species have the most potential as ethanol stock. They initially will test a variety of grasses and woods, including poplar and willow, two fast-growing trees. Another set of tests will determine ‘‘which tissues are more optimal for ethanol production, stems or leaves or roots,’’ Smith said. Eventually, the testing will include different growing conditions, including more or less sunlight and water availability, and they will determine the best time for harvest. If Smith’s laser-beam fingerprinting works for biofuel crops, it can be used to identify other plant materials that could be used to produce pharmaceuticals, plastics and just about anything else.