Remote sensing of soybean canopy to identify calcareous soils Natalia Rogovska and Alfred Blackmer, Dept. of Agronomy, Iowa State University Plot 1 Introduction Soybean plants often show symptoms of iron deficiency chlorosis on calcareous soils. Such soils often occur in complex spatial patterns, which are difficult to characterize. Plot 1 Plot 2 Plot 2 Observations of spatial patterns in plant size (and canopy cover) within fields as revealed by aerial photographs suggested that plant size may be correlated with carbonate concentrations and, therefore, that patterns in plant size may give some indication of spatial patterns in soil carbonate content. Especially in fields where soybeans were planted in rows 76 cm apart, spatial patterns in plant growth could be easily characterized by analysis of color, which is primarily determined by the amounts of soil showing between rows. As shown by example in Fig. 1a, the amount of soybean growth often ranged from complete canopy cover to no canopy cover due to death of soybean seedlings. Plot 3 Plot 3 Plot 4 Although spatial patterns in plant growth often resemble boundaries on soil map units, the spatial patterns in soybean growth occurred in much finer scales than soils are mapped. The patterns in soybean growth often showed continuous gradation rather than the distinct boundaries imposed by the normal categorical classification of soils into map units. In order to study calcareous soils it is important to map them correctly. The objective of this study was to understand how remote sensing of soybean canopy can be used to identify and map calcareous soils. Materials and methods Soybean fields that offer great variability in plant height and canopy closure as revealed by aerial images were selected in central Iowa. Aerial images of 3 fields (RGB and NIR) were taken in the mid July and georeferenced using ArcView GIS software. Green Normalized Difference Vegetation Index (GNDVI), which is related to the proportion of photosynthetically absorbed radiation, was calculated from NIR images using ArcView (Fig. 1b). 160 y=249.5-18.5X r2 = 0.86, p<0.0001 GNDVI 140 100 80 60 6 7 8 Stress Index 9 Plot 5 Flooded areas a Plot 5 b c Figure 1. Different levels of stress imposed on plants as revealed by a: aerial image, b: GNDVI, c: reclassification of GNDVI into different classes. GNDVI was then reclassified into different classes (usually six) or levels of plant growth (Fig.1c). Three to four soil samples were taken from different GNDVI classes and analyzed for pH and calcium carbonate equivalent (CCE). A newly developed Stress Index (SI), which combines effects of pH and CCE (Rogovska N. 2004. Soil pH and carbonate effects on soybean yield. M.S. thesis, Iowa State University), was calculated using the equation SI=pH+0.14CCE Results and discussions Regression analysis showed statistically significant (p<0.001) correlations between Stress Index and GNDVI in all three fields. The r2 ranged from 0.54 to 0.86 with a mean of 0.75. The graph and image show examples of analyses done at one field. 120 5 Plot 4 10 Remote sensing of plant canopies offers a promising way to select fields and sampling points within fields. This approach minimizes sampling problems caused by variation in soil properties that occur in complex spatial patterns within a field because remote sensing essentially classifies soils before samples are collected. Regression analyses between measured soil properties and amount of biomass, which closely relates to soybean yields, are used to define relationships between soybean yield and stresses imposed by calcareous and high pH soils.