Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Water Quality Assessment of Karingal Yalloc: Water Chemical and Macro Faunal Testing By Zara Aghajanyan 1 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Objective_____________________________________________________________ To define the current state of the Karingal Yalloc, a small urban creek in Eltham, Melbourne. By analyzing chemical and biological indicators of water pollution a baseline will be generated. Introduction_________________________________________________ _________ Nerreman Gateway was once a large block of farmland, owned by the Smith family. What once was a hobby farm has now been turned into a wetlands. A storm water fed creek, named Karingal Yalloc, runs through the block of land, parallel to Coolabindi Chase. When the land was still farmed a large dam was built to block the creek and to create a lake. As the dam was first of its kind it is currently heritage listed and cannot be changed. The creek is in an urban area and numerous factors, including the retardation of stream flow by the dam itself, degrade the water quality of the small creek. Pollution from stormwater drains, runoff from the surrounding neighborhood, anoxic conditions within the dam and heavy industrial pollution, such as the collapse of a road into the creek, degrade the water quality significantly. This report aims to generate the current water quality baseline of the creek and to relate it to past observations. This will further help in creating the Nillumbik Indigenous Plants Park along Karingal Yalloc. Brief History_______________________________________________ _________ Dam built in 1940 by Douglas Bernhard Smith Farming ceased by end of 20th century, urban settlement began Waterwatch along Karingal Yalloc began in 2001 Collapse of Sherbourne road into creek on Febuary 2005 Native reforestation along length of creek from Weidlich Rd to Gleneagle way, 2002 – 20 Lake dredged due to bad odour and invasive Cumbungi reed largely removed, March 2010 Application for the creation of the Nillumbik Indigenous Plants Park, 2015 Collapse of Sherbourne road into Karingal Yalloc in 2005. Photo courtesy of Julia Davis 2 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Methods ___________________ __ Four sampling locations were chosen along the creek, both upstream and downstream from the Smith dam. This was done to minimize bias and to get a full understanding of the health of the creek. Location 3 was located downstream from two stormwater drains and was chosen to give a better understanding of the amount of pollution in the storm water. Section 2 of Karingal Yalloc Sampling locations along creek Water chemistry The Thermo Fisher 90FLT Field Analyzer was used for accurate water chemistry measurements in the field. The analyzer came with numerous water proof probes that each measured a different parameter. The probes were left in shallow water for a few moments and the results were recorded on the analyzer box. This was repeated 3-4 times at each sampling spot to minimize error. Initially other field testing kits were used, such as the LaMotte D.O Kit, TDScan3 and the Waterwatch Turbidity Tube, but they were proven to be inefficient and unreliable, for this reason they are not included in this report. The Total Phosphorous was measured using the Merck Oxisolve/Aquaquant Test Kit. Previous Waterwatch Victoria reports on Karingal Yalloc were accessed and used to compare the current state of the creek to and use as reference. The Waterwatch Turbidity Tube relied heavily on interpretation and was deemed unreliable 3 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Macroinvertebrates Macroinvertebrates were manually collected using a long Dframe net by vigorously sweeping vegetation and debris in shallow water, such as reeds, fallen logs and tree roots. The sample was rinsed by pouring stream water through it or by sweeping open water to get rid of silt and mud particles. The contents of the net were emptied out onto a large plastic tray and stream water was poured on top to dislodge the organisms from the net. Using tweezers and plastic pipettes organisms were individually plucked from the tray and separated into ice cube trays for identification. The identification book by Gooderham and Tsyrlin (2002) of freshwater invertebrates was used to categorize the organisms under their respective SIGNAL score. SIGNAL stands for Stream Invertebrate Grade Number – Average Level. This method of rating helps to understand the amount of disturbance an ecosystem has undergone, the more polluted the water is – the more tolerant types of bugs will be present. The rating is as follows: Collecting macroinvertebrates from shallow grass roots Higher than 6: healthy habitat Between 5 and 6: mild pollution Between 4 and 5: moderate pollution Less than 4: severe pollution 4 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Results ___________________ __ Table 1. Collected water chemical parameters which aid to assess the current baseline of the creek and the amount of disturbance affecting it. Date: Location: Notes: 22/04/15 1a. Top of dam (Nerreman gateway) 20o C, 20% foliage, shade cover. 3pm Sample T (C) DO (ppm) 1.1 14.5 1.2 1.3 Location: Notes: EC pH Turb (NTU) TP (mg/L) 0.5 DO (% sat) 5 131 6.05 14-20 0.04 15 0.22-0.4 2-4 138 6.4 11 0.06 14.5 0.55 5 135 6.4 12-19 0.06 1b. Top of dam, opposite bank (Karingal drive) 10% foliage cover, still water 1.4 14.8 0.77 8 132 6.61 17-25 0.07 1.5 15.3 0.64 6 116 6.6 14-25 0.04 1.6 13.8 0.83 8 90 6.63 10-26 0.04 Location: 2. Bottom of dam, across Gleneagle Way Notes: 40% foliage, shade cover, running water 2.1 14.6 4.6 45 70 6.87 5 0.04 2.2 14.7 4.74 47 70 6.9 5-6 0.04 2.3 14.6 4.45 44 77 6.9 6.2 0.04 Location: Notes: Sample 3.1 3.2 3.3 3.4 Location: Notes: 4.1 4.2 4.3 3. Two estuaries combine (Weidlich road) 5-10% foliage, cloud cover, 3pm. Lots of gravel in creek bed. Running water. T, C DO DO EC pH Turb TP (ppm) (% sat) (NTU) (mg/L) 15.7 8-8.5 80-85 163 7.6 7-10 0.08 15.9 9.3 94 160 7.58 7-8.5 0.07 15.3 7.16 71 163 7.8 8-15 0.07 16.5 9.2 94 156 7.87 10-12 0.07 4. 50-70m down from estuaries (Ramptons road) 10-15% foliage cover. Cloud cover. Running water 15.3 4.7 47 131 7.28 15 4.8 48 115 7.4 15.1 5-5.5 50-55 124 7.6 5 5-10 6~8 0.05 0.05 0.05 5 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Figure 1. Visual representation of counted macroinvertebrates. Total count of EPT taxa: 4. General range of signal grades: ~1-3. See: Appendix 1. Figure 2. Past signal grade distribution with the most abundant grades (1-3). See: Appendix 3 6 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Figure 3 Dissolved oxygen saturation history of the lake above Smith dam on Nerreman Gateway. Likely correlation between temperature and DO saturation, higher DO concentrations due to lower temperatures. See Appendix 2 Figure 4 Slight positive correlation between rainfall and DO. R2 indicates how well the data fits the regression line. As it is very low, the correlation between the two parameters is very weak. 7 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Figure 5. Abundance of phosphate and ammonium in the past. Notable spike followed by decrease in concentration after dredging of dam in March 2010. 8 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Discussion ___________________ __ The Australian and New Zealand Guidelines for Fresh and Marine Water Quality (ANZECC) standards and the Waterwatch Victoria standard for freshwater environments will be used as reference points to compare the collected data. ANZECC (2000) guidelines for slightly disturbed and highly disturbed streams: Environment Phosphorous (mg/L) Nitrogen (mg/L) Lowland River 0.02 Freshwater Lake 0.005 0.02 0.01 DO (% sat) pH Min Max 85 110 8 90 110 8 Salinity Turbidity (µS/ cm) (NTU) 125 20-30 6-50 1-20 Waterwatch guidelines for freshwater streams: Parameter Conductivity (µS/ cm) Turbidity (NTU) pH Excellent <100 <15 6.0-7.5 Good <250 <17.5 5.5-6 or <8.0 Fair <500 <20 8.0-8.5 Phosphorous (mg/L) Ammonium (mg/L) <0.008 <0.05 <0.02 <0.1 <0.04 <0.2 Poor <750 <30 5.0-5.5 or 8.5-9.0 <0.08 <0.4 Degraded >750 >30 <5.0 or >9.0 >0.08 >0.4 The macroinvertebrate data immediately shows that the environment is heavily polluted as the SIGNAL scores generally range from 1-3 (Fig 1). Previous testing also had the same range of scores, with the upper limit occasionally being a score of 5 or 6 (Fig 2, Appendix 3).Though, in more recent time the amount of less resistant bugs have become more abundant. In just one month 4 specimens of EPT (Ephemeroptera, Plecoptera, Trichoptera) taxa were found, which is a sure sign that the conditions have improved as these indicator insects have SIGNAL scores of 8-10 (Gooderham and Tsyrlin 2002). This may correlate to the recent increase in native reforestation along Karingal Yalloc by the Nillumbik council (Appendix 4). Numerous shells of Ephemeroptera nymphs were found regularly at the sampling sites, which is also a sign of improving conditions. Above the Smith dam and along Nerreman gateway the dissolved oxygen (DO%) saturation was significantly more degraded than at other sections along the creek, ranging from 2-8 (Table 1). This is critically under saturated, as the lower limit for DO% in the ANZECC guidelines is 90. Both the retardation of stream flow by the dam and the invasive reeds upstream severely affect the DO saturation of the lake. Decreased stream flow would cease turbidation and aeration of the stream, whilst the invasive reeds choke the stream and end water circulation. As the Smith dam is heritage Common waterboatman (Corixidae), SIGNAL score: 3 9 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com listed (Victorian Heritage Database, 2010), one solution would be to increase the outflow from the system to increase stream flow. Excessive and repeated weeding of the area would keep the reeds under control and increase the thermal convection of water, which itself would create flow and aeration. Further reforestation can introduce more native plant species to the stream and increase the competition against the invasive reeds. A fountain or some boulders can increases the turbulent flow of the water and aid in aeration (IEPA 1997). It should also be mentioned that the DO% can be dependent on the temperature of the water (US EPA, 2012). Colder water can hold higher concentrations of oxygen (Fig 3). Decreased DO% can cause algal growth and cyanobacteria blooms which can further deplete the water body of oxygen and create anoxic conditions. Decrease in DO% may also reduce the redox potential and suspend heavy metals from sediments, which has adverse effects on the ecosystem. Further downstream and upstream the DO% ranges from poor to fair, which can be attributed to rainfall and urban pollution. The ammonium was too low to generate a reading, while the total phosphorous was above both guidelines. It ranged 0.04 -0.08, which is above all guidelines and can be attributed to either the storm water runoff or the past land use. Also previously within the Eltham area primarily septic tanks were used to store and treat wastewater. Septic run off would have also caused an increase in nutrient concentrations within the creek and in the surrounding sediment. In the past both ammonium and phosphorous have been quite high (Fig 5). This can be related to the invasive weeds within the water or algal blooms, which both decrease the water quality of the lake (EPA VIC, 2001). Increase in DO% and extensive weeding of invasive reeds will decrease the occurrence of harmful algal blooms. Increased nutrient concentration and a decrease in DO% in freshwater systems can be the catalyst to a self-sustaining toxic cycle. Nutrients can cause algal growth and cyanobacterial blooms which reduce light penetration into the water and hinder photosynthesis of aquatic plants. The increase of organic detritus and the resulting decay of organic material further decreases the DO concentration within the water, which smothers aquatic organisms – particularly fish (ANZECC 2000). In the past scum and bad odour have been noted below the dam wall, which could be due to toxic bacteria, such as cyanobacteria, that thrive in anoxic and nutrient rich environments. Scum below the dam wall. Photo courtesy of Julia Davis, Waterwatch The electrical conductivity (EC) indicates the amount of dissolved ions, such as salts, Calcium, Magnesium, Potassium, Chlorides and Bicarbonates in a water body. Increased conductivity in Karingal Yalloc can be attributed to storm water that feeds the creek (Fig 4) or runoff from the major roads surrounding it. The EC within all sections of the creek is relatively good according to the Waterwatch guidelines (<250), but slightly high according to ANZECC standards. Previously the EC has been poor to degraded according to Waterwatch and very degraded by ANZECC (Appendix 2). An increase in salinity may cause a release of heavy metals within the water body from surrounding sediment. This may drastically harm the ecosystem, affecting the anatomy and reproductive success of aquatic organisms (WATERSHEDSS, 1995). 10 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com The turbidity of all the sites is quite low, meaning the suspended particles within the water bodies are very low. This is a positive sign as increased turbidity impedes on photosynthesis within the creek which can lead to toxic environments. Previously the turbidity has been quite constant, ranging from 10-50, with occasional spikes during increased rainfall, which temporarily mobilizes solids. The collapse of Sherbourne road into the creek would have also negatively affected the turbidity. The measured pH was quite good, but in the past the pH has varied significantly (Appendix 2). This can be attributed to the acidity or alkalinity of the stormwater that feeds the creek, the amount of suspended heavy metals in the water or even the amount of physical rubbish left in the creek. Decrease in pH may cause the release of heavy metals from sediment into the water (WATERSHEDSS, 1995). As aforementioned, this has very negative impacts on the health of the ecosystem. Systematic and Random Errors _____ __ It should be note that numerous errors could have altered the collection of data. These could range from random, those that could not be controlled by the person collecting the sample, to systematic – errors that can be controlled. Some examples of these errors: Random Weather: increased rainfall, sunshine, shade cover Systematic Incorrect use of equipment Organisms: abundance depending on season Interpretation of samples: identification of bugs dependent on interpretation Surrounding environment: landslide, increased pollution from industrial development, increased rubbish Not enough repeated samples, to minimize bias Also the use of SIGNAL scores should not be used solely when analyzing freshwater environments. An ecological system could be in balance, but have more tolerant species that thrive in it. E.g. bugs that live in brine environments. Or be abundant in more sensitive organisms, but in a very niche habitat (Gooderham and Tsyrlin 2002). Conclusion ___________________ __ Karingal Yalloc can be labelled as a ‘highly disturbed creek’ (ANZECC, 2000). Stormwater drainage and urban runoff from the surrounding suburbs heavily influence the health of the creek. By further reforestation and weeding the condition of the creek can be greatly improved. Further increase of the output from the dam will also positively alter the chemical makeup of Karingal Yalloc and help to return the natural ecosystem to a balanced state. If increased output from dam is not possible, other forms of aeration must be implemented, such as a fountain. 11 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com References _______________ __ ANZECC (2000) Australian and New Zealand Guidelines for Fresh and Marine Water Quality, ‘National Water Quality Management Stategy. EPA VIC (2001) Nutrient objectives for rivers and streams – ecosystem protection | EPA Victoria. Viewed on 02/07/2015 at: < http://www.epa.vic.gov.au/our-work/publications/publication/2001/december/792-1> Gooderham, J., and Tsyrlin, E. (2002) The Waterbug Book, CSIRO Publishing, Australia Illinois EPA (1997) Lake aeration and circulation. Viewed on 02/06/2015 at <http://www.epa.state.il.us/water/conservation/lake-notes/lake-aeration.pdf> US EPA (1997) How do we measure the quality of our waters? Viewed on 02/07/2015 at: < http://water.epa.gov/learn/resources/measure.cfm> Victorian Heritage Database (2010) Smith Dam. Viewed on 06/07/2015 at: <http://vhd.heritage.vic.gov.au/places/result_detail/63471> WATERSHEDSS (1995) Precipitation of Heavy Metals from Wastewaters. Viewed on 06/07/2015 at: < http://www.water.ncsu.edu/watershedss/info/hmetals.html> Acknowledgements __________ __ A big thank you to my helpers Stephanie Eastmure, Catherine Comerford and Taron Naoroji. Thank you to the Friends of Karingal Yalloc, Alex Smith and Theodoros Papakonstantinou for organizing this opportunity. Special thanks to Ian Burns and Julia Davis for supplying extensive data and information on the history of the site. Also thank you to Karen Borton and the Nillumbik Shire Council for supplying the field equipment. 12 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Appendix 1: Macroinverterbate Count Date: Location: Notes: Name Odonata Diptera Tubificid worm Platyhelminthes Turbellarians Hirudinea (leach) Collembola (springtail) Stratiomyids Corixidae (waterboatmen) Location: Name Diptera Tubificid worm Collembola (springtail) Corixidae (waterboatmen) Plecoptera (stone fly) nymph Date: Location: Notes: Name Heteroptera (water striders) Platyhelminthes Turbellarians Tubificid worm Springtail Hirudinea (leach) Corixidae (waterboatmen) Diptera Ephemeroptera (mayfly) Odonata (damselfly) 4/2/2015 1a. Top of dam Sunny, partly cloud cover. 20-25oC Count 5 45 15 7 7 16 2 2 Signal Grade 3 3 2 2 1 1 2 2 1b. Top of dam, 10m up stream Count Signal Grade 17 3 10 2 8 1 6 2 1 10 12/2/2015 2. Below dam, across Gleneagle Way Warm, 25o C, light cloud cover Count 23 50+ 37 50+ 15 7 20 1 1 Signal Grade 2 2 2 1 1 2 3 9 3 13 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Location: Name 3. Two estuaries combine. (Weidlich road) Count Signal Grade Odonata 1 3 Acarina (freshwater mite) 6 3 Ephemeroptera 1 6 Platyhelminthes Turbellarians Gastropoda (fresh water snail) Diptera 6 2 18 2 50+ 1 Corixidae (waterboatmen) 3 3 Tubificid worm 9 2 Collembola (springtail) 15 2 Note: Abundance of abandoned shells of ephemeroptera nymphs. Date: Location: Notes: Name Odonata Corixidae (waterboatmen) Diptera Platyhelminthes Turbellarians Tubificid worm Acarina (freshwater mite) Ephemeroptera (mayfy) Heteroptera (water striders) Hirudinea (leach) 7/3/2015 4. Further downstream of estuaries (Ramptons road) Cloud cover, warm, mild wind. Count Signal Grade 1 3 7 2 4 3 50+ 2 26 2 2 6 1 9 12 2 3 1 14 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Appendix 2: Past Water Chemistry Data from Nerreman Gateway Date 09-2001 05-2006 06-2006 07-2006 08-2006 09-2006 10-2006 11-2006 12-2006 02-2007 03-2007 04-2007 05-2007 06-2007 07-2007 08-2007 09-2007 10-2007 11-2007 12-2007 02-2008 04-2008 05-2008 06-2008 07-2008 08-2008 09-2008 Ammonium (mg/L NH4+) 9.8 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A 0.2 N/A 0.2 0.15 0.2 0.01 0 0 0 0 0 0 0 0.01 0.08 DO (% SAT) 0 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A 39 74 53 35 39 41 32 37 41 54.45 37.96 53.59 73 53 55 DO (mg/L) 95.96 N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A 4 8 6 4 4 4 3 3.5 4 5.5 4 6 8 7 6 EC (µS/cm) 1280 N/A N/A N/A N/A N/A 1290 600 N/A N/A N/A N/A 360 240 730 580 440 360 490 580 180 300 350 470 370 440 340 pH 7.8 N/A N/A N/A N/A N/A 7.6 7.4 N/A N/A N/A N/A 6.9 7.1 7.1 8 6.5 7.4 6.6 6.5 8 7.1 7.2 7.2 7.7 7.5 7 Phosphate (mg/L) 0.0326 N/A N/A 0.06 0.05 0.06 0.06 0.15 0.1 0.07 0.03 0.02 0.03 0.07 0.03 0.05 0.125 0.08 0.1 0.08 0.05 0.15 0.07 0.1 0.08 0.06 0.06 T (water oC) 14 15 12 10.5 11.4 12 15.4 19.6 18.2 21 18.9 14.9 13.8 11.3 9.5 9.4 14.1 15.5 18 18 16 14.5 12.6 10 11 9.2 11 Turbidity (NTU) 12 35 10 30 15 10 10 20 20 10 10 10 10 60 40 10 10 20 15 10 15 10 20 10 15 10 10 Weather Cloudy Raining Sunny Cloudy/ windy Sunny/ cloudy Sunny/ windy Cloudy/ windy Sunny/ smoke haze Overcast/ windy Sunny/ windy Sunny Cloudy/ windy Cloudy Overcast/ windy Cloudy/ windy Cloudy/ windy Cloudy/ windy Sunny Cloudy, windy Overcast Sunny Sunny/cloudy Overcast Sunny/cloudy Sunny/cloudy/windy Sunny/cloudy/windy Rain (mm/mo) 40 50 45 51 33 34 23 26 54 30 52 22 52 50 51 22 8 12 74 43 20 31 12 12 47 60 29 15 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Date 10-2008 11-2008 12-2008 01-2009 01-2009 03-2009 04-2009 05-2009 06-2009 07-2009 08-2009 09-2009 10-2009 11-2009 12-2009 01-2010 02-2010 02-2010 03-2010 04-2010 05-2010 07-2010 08-2010 09-2010 10-2010 12-2010 01-2011 03-2011 04-2011 Ammonium (mg/L NH4+) 0.07 0 0.1 N/A 0 0.07 0.04 0 0.02 0 0 0.15 0.08 0.8 0 0.01 0.4 0.03 0.15 0.05 0.45 0.09 0.01 0.07 0.01 0 0 0 0.01 DO (% SAT) 38 41 42 36 41 36 106 36 37 55 72 28 37 34 44 35 36 35 47 42 27 57 72 39 42 66 39 33 50 DO (mg/L) 4 4 4 3.5 3.5 3.5 10 4 4 6 8 3 4 3 4 3 4 3 4 4 3 6 8 4 4 6 3.5 3 5 EC (µS/cm) 270 200 340 390 630 340 130 200 150 310 90 130 350 980 460 290 850 440 470 180 280 390 170 310 780 1080 1400 420 1200 pH 7.4 7.1 7.3 6.9 7.1 6.6 6.4 6.7 7.1 6.5 6.2 6.2 6.6 6.9 6.9 6.9 7.4 6.9 6.6 6.9 6.9 7.7 8.1 7.4 7.6 7.8 7.5 7.3 7.6 Phosphate (mg/L) 0.05 0.03 0.1 0.1 0.025 0.15 0.5 0.03 0.08 0 0.07 0.07 0.04 0.12 0.06 0.06 0.07 0.1 0.07 0.07 0.08 0.08 0.05 0.05 0.06 0.08 0.03 0.05 0.07 T (water oC) 12.2 15.7 17.8 16.5 23 16 17.8 10.5 12 11.4 10.5 12 11.6 20.9 20 22.8 9.9 22.2 23.4 17 11 12.4 10.1 13.6 17.5 19.3 20.9 20.1 14.9 Turbidity (NTU) 10 20 15 15 10 15 400 20 20 15 50 20 10 20 20 50 15 20 10 20 15 22 30 20 11 25 10 25 10 Weather Overcast Sunny/cloudy/windy Sunny/cloudy/windy Overcast/raining/windy Sunny Sunny/windy Raining Overcast/windy Sunny/cloudy Sunny/ windy Overcast/ windy Sunny/windy Sunny Sunny/windy Sunny/windy Sunny/windy Overcast Sunny/windy Sunny/windy Sunny/ windy Cloudy Cloudy/ windy Cloudy Cloudy/ windy Overcast/ windy Cloudy/ windy Overcast/ windy Cloudy Sunny/ windy Rain (mm/mo) 12 82 90 0 0 45 64 8 51 32 48 117 117 82 69 39 37 37 57 46 40 37 71 51 154 97 109 43 119 16 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Date 05-2011 07-2011 07-2011 09-2011 09-2011 10-2011 11-2011 12-2011 02-2012 03-2012 06-2012 07-2012 09-2012 10-2012 01-2013 02-2013 04-2013 05-2013 05-2013 07-2013 08-2013 08-2013 09-2013 10-2013 11-2013 12-2013 02-2014 03-2014 04-2014 05-2014 Ammonium (mg/L NH4+) 0.06 0.07 0.02 0.35 0.07 0.07 0 0.15 0.4 0.02 0.15 0.07 0.085 0.1 0.06 0.06 0 0.05 0.07 0.08 0.03 0.08 0 0 N/A 0.05 N/A 0.15 0.4 0.1 DO (% SAT) 68 54 95 88 56 78 69 43 35 33 55 50 56 67 38 51 31 76 88 47 47 93 79 48 65 56 33 31 30 34 DO (mg/L) 7 6 10 9 6 7 6 4 3 3 6 6 6 3 3.5 5 2.5 8 9 5 5 10 8 5 6 5 3 3 3 3.5 EC (µS/cm) 320 1190 250 560 1130 750 250 700 510 550 690 1200 560 500 1030 170 440 130 200 540 960 370 230 370 210 230 250 320 300 7.3 pH 7.3 7.7 7.2 7.3 7.7 7.5 7.8 7 6.8 6.9 7.1 7.3 7.3 7.2 7.1 6.3 6.9 6.9 7 7.2 6.9 7.8 6.6 71 6.3 7 5.9 6.2 6.8 7 Phosphate (mg/L) 0.04 0.07 0.025 0.1 0.05 0.125 0.05 0.085 0.1 0.07 0.07 0.08 0.06 1.36 0.04 0.06 0.09 0.07 0.07 0.07 0.06 0.04 0.03 0.03 0.07 0.15 N/A 0.12 0.15 0.14 T (water oC) 13.5 10.4 12.7 13.8 14.1 20.4 22.2 18.1 22.1 18.9 10.8 7.1 11.7 20.2 18.9 16.3 26.4 12.9 14 12.4 12.1 11.5 14.4 13 19.1 20.3 19.9 16.7 15 14 Turbidity (NTU) 50 15 15 27 9 40 100 14 14 9 13 9 13 9 9 21 13 19 30 10 13 24 9 13 14 30 30 19 10 9 Weather Cloudy/ windy Sunny/ windy Sunny Sunny/ windy Sunny/ windy Cloudy/ windy Raining/ windy Cloudy Windy Windy Cloudy/ windy Raining Overcast/ windy Sunny/ windy Cloudy/ windy Sunny/ windy Sunny Overcast/ windy Raining Cloudy/ windy Sunny/ windy Cloudy/ windy Cloudy/ windy Overcast/ windy Cloudy/ windy Sunny/ windy Raining Overcast/ windy Overcast/ windy Cloudy/ windy Rain (mm/mo) 62 47 47 87 87 58 139 161 78 61 54 75 35 36 17 70 23 35 62 40 58 69 61 50 72 18 19 30 31 36 17 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Appendix 3: Past Macroinvertebrate Counts, outliers have been highlighted Date 05-2001 06-2001 08-2001 09-2001 09-2001 03-2006 04-2006 05-2006 06-2006 08-2006 09-2006 10-2006 11-2006 12-2006 02-2007 03-2007 04-2007 05-2007 06-2007 07-2007 08-2007 09-2007 11-2007 12-2007 02-2008 04-2008 05-2008 06-2008 1 1 2 0 11 8 3 12 10 2 9 7 10 14 16 1 0 1 4 6 2 2 13 1 8 5 4 1 4 2 7 0 1 29 55 1 15 12 10 6 8 12 11 28 12 3 4 17 9 7 9 13 6 44 25 20 15 6 3 4 1 7 0 3 11 4 9 32 7 10 18 15 41 12 0 3 12 7 2 3 45 54 22 13 36 17 32 4 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Signal Grade 5 0 1 0 0 0 0 1 0 0 0 1 0 0 20 0 0 0 0 0 0 0 0 6 3 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 18 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Date 07-2008 08-2008 08-2008 10-2008 11-2008 12-2008 01-2009 01-2009 03-2009 04-2009 05-2009 06-2009 07-2009 08-2009 09-2009 11-2009 12-2009 01-2010 02-2010 02-2010 03-2010 04-2010 05-2010 07-2010 08-2010 09-2010 10-2010 12-2010 01-2011 02-2011 1 16 1 3 5 8 9 6 1 10 8 5 3 2 1 6 0 5 7 3 4 3 2 10 9 10 25 68 24 32 12 2 6 12 7 18 50 14 59 14 49 7 33 41 54 33 37 17 41 53 36 44 19 80 70 50 19 20 27 49 50 85 3 21 5 5 26 41 100 32 5 60 0 9 0 4 15 6 104 40 72 0 20 38 35 23 20 30 3 18 18 150 12 Signal Grade 4 5 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 1 0 4 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 10 6 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 3 8 0 0 0 0 0 0 0 0 0 1 0 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 19 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Date 03-2011 04-2011 05-2011 07-2011 07-2011 09-2011 10-2011 12-2011 02-2012 03-2012 03-2012 06-2012 07-2012 08-2012 09-2012 10-2012 02-2013 02-2013 04-2013 05-2013 07-2013 08-2013 09-2013 10-2013 11-2013 02-2014 03-2014 04-2014 05-2014 02-2015 02-2015 1 12 7 8 7 3 26 17 85 3 10 27 3 4 7 9 43 10 5 5 0 6 4 6 15 13 32 4 0 6 23 8 2 47 9 18 17 18 29 24 44 33 93 42 58 39 40 35 31 94 78 67 24 29 8 20 21 21 59 47 25 14 26 16 3 150 36 13 40 103 115 35 39 150 37 111 16 43 95 107 43 124 103 12 13 11 8 93 18 19 59 12 24 0 50 17 Signal Grade 4 5 0 0 0 1 0 0 0 0 0 0 0 10 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 1 0 0 0 7 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 8 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 9 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 20 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Appendix 4: Plants Inventory Nillumbik Indigenous Plants Park – species planted Courtesy of Friends of Karingal Yalloc 1. A. acinacea 32. E. ovata 2. A. dealbata 33. E. viminalis 3. A. genistifolia 34. Eleocharis acuta 4. A. implexa 35. Ficinia nodosa 5. A. mearnsii 36. Glycine latrobiana 6. A. melanoxylon 37. Goodenia ovata 7. A. paradoxa 38. Goodia lotifolia 8. A. pycnanthra 39. Hardenbergia violacea 9. A. verticulata 40. Indigofera australis 10. Acaena novae-zelandiae 41. Kennedia prostrata 11. Arthropodium strictum 42. Kunzea 12. Austrostipa mollis 43. Linum 13. Austrostipa pubinodis 44. Lomandra longifolia 14. Austrostipa scabra 45. Melicytus dentatus 15. Banksia serrata 46. Microlaena stipoides 16. Billarderia scandens 47. Olearia stellata 17. Bulbine bulbosa 48. Ozothamnus ferrugineus 18. Bursaria spinosa 49. Platylobium obtusangulum 19. Callistemon sieberi 50. Poa ballardieri 20. Carex appressa 51. Poa ensiformis 21. Carex tereticaulis 52. Pomaderris aspera 22. Cassinia aculeata 53. Pultenaea pendulata 23. Cassinia longifolia 54. Pultinaea daphnoides 24. Chrysocephalum semipapposum 55. Pultinaea gunnii 25. Clematis microphylla 56. Rubus parvifolius 26. Correa 57. Solanum laciniatun 27. Danthonia setacea 58. Themeda triandra 28. Dianella longifolia 59. Solanum lociniatum 29. Dianella revoluta 60. Themeda manda 30. Dichelachne 61. Vimianarea uncea 31. E. meliodora 21 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Current Plants Inventory Courtesy of Chantelle Ebony Roberts and Georgia Lloyd, Monash University 2015 Shrubs Scientific Name Acacia genistifolia. Acacia paradoxa Acacia suaveolens Acacia verticillata Bursaria spinosa Cassinia aculeata Cassinia arcuata Correa lawrenciana Dodonaea viscosa Goodenia ovata Grevillea rosmarinifolia Gynartix pulchella Hakea decurrens Helichrysum luteoalbum Hymenthera dentata Leptospermum continentale Leptospermum phylicoides Melicytus dentatus Olearia phlogopappa Olearia species Pomaderis lanigera Pomaderris aspera Prostanthera lasianthos Solanum naciatum Common Name Spreading wattle Hedge wattle Sweet Wattle Prickly Moses Sweet Bursaria Dogwood Drooping Cassinia Mountain Correa Giant hop bush Hop Goodenia Spider Flower Hemp bush Bushy needlewood Weed Tree violet Prickly tea tree Burgan Tree Violet Dusty Daisy bush Wooly Pomaderis Hazel Pomaderris Mint bush Kangaroo apple 22 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Grasses Scientific Name Acaena novae-zealandiae Actotheca calendula Anthoxanthum odoratum Austrostipa rudis Briza maxima Carex appressa Cirsium vulgare Delairea odorata Ehrhata erecta Microlaena stipoides Nassella neesiana Poa labillardieri Poa sieberiana Rytidosperma fulvum Rytidosperma racemosum Themeda triandra Common Name Bidi-bidi Cape dandelion Buffalo grass Veined spear-grass Quaking grass Tall sedge Spear thistle Cape ivy Panic veldtgras Weeping grass Chilean needle grass Tussock grass Grey tussock-grass Wallaby grass Wallaby grass Kangaroo grass Status Native Weed Weed Native Weed Native Weed Weed Weed Native Weed Native Native Native Native Native Common weeds found throughout: Actotheca calendula Allium triquetrum Anthoxanthum odoratum Briza maxima Cirsium vulgar Cynodon dactylon Delairea odorata Ehrhata erecta Helichrysum luteoalbum Poa annua Taraxacum officinale Veronica persica 23 Zara Aghajanyan 2015, Monash University, Victoria 3800, Australia, zaghaj@gmail.com Trees Scientific Name Allocasuarina littoralis Acacia mearnsii Acacia melanoxylon Eucalyptus dives Eucalyptus rubida Exocarpos cupressiformis Melaleuca armillaris Acacia pycnantha Acacia implexa Eucalyptus goniocalyx Eucalyptus viminalis Eucalyptus radiata Melaleuca styphelioides Eucayptus sideroxylon Eucalyptus macrorhyncha Acacia dealbata Eucalyptus ovata Melaleuca ericifolia Hakea salicifolia Eucalyptus melliodora Common Name Black Sheoak Black Wattle Blackwood Broad-leaved Peppermint Candlebark Cherry Ballart Giant Honey Myrtle Golden Wattle Lightwood Long-leaved Box/Bundy Manna Gum Narrow-leaved Peppermint Prickly-leaved Paperbark Red Irondbark Red Stringybark Silver Wattle Swamp Gum Swamp Paperbark Willow-leaf Hakea Yellow Box 24