AcroPrep™ Advance Filter Plates
for Neonatal Screening
Enhanced performance and improved consistency
ensure accurate neonatal screening results
High performance membrane – Effectively holds back fibers from dried blood spots which can interfere
with the optical density readings.
New outlet tip geometry – Minimizes sample leakage and loss during incubation steps, decreasing
the time required for sample retesting and second screens.
Optimized well design – Provides consistency in filtration times, ensuring reproduciblity lot after lot.
Sample cross contamination minimized – New outlet tip design reduces the presence of hanging
drops following filtration.
Low non-specific binding – Prevents target analytes from binding and interfering with assay performance.
100% visual inspection – Ensures each well has proper membrane placement and is free from debris.
Automation compatible – Manufactured in accordance with SBS guidelines, allowing plates to be run
in manual, semi-automated, and automated processes.
Applications
Neonatal screening of functional and genetic disorders
Sample preparation for neonatal screening assays such as BIOT, GALT and TGAL
Figure 1
Efficient Filtration Times with Optimized Membrane Configurations
Evacuation Time (sec)
50
45
100
80
60
40
Pall
Lot O
Lot M
Lot N
Lot K
Lot L
Lot I
Lot J
Lot H
Lot E
Lot F
Lot G
0
Lot C
20
Lot D
Critical to the performance of the
filter plate in the
diagnostic determiProtein Precipitation Occurs in Plate
nation of neonatal
screening disorders
Yes - PN 8060
No - PN 8079
Biotinidase Plates
Neonatal Screening Plates
is the performance
of the membrane.
The membrane configurations used in Pall’s AcroPrep Advance
filter plates have been optimized for the precipitation process,
whether it happens off-line or in the plate; thereby helping to
ensure complete sample transfer for analysis.
Sample Prep Method
120
Lot A
Lot B
Robust and consistent performance of the filter plates is a key
element to providing accurate screening results. Variability in
plate performance introduces the need for repeat testing and
additional costs, as well as delays in a diagnosis for the newborn.
The AcroPrep Advance filter plates have been optimized for
use in newborn screening tests and demonstrate reproducible
performance ensuring results that can be trusted.
Figure 2
AcroPrep Advance Filter Plates Provide Consistent
Biotinidase Response
Expected Biotinidase Response (%)
Performance
Plate
Competitor M
Fifteen lots tested of a competitor’s filter plates (shown in blue) were
found to decrease the expected biotinidase activity in the specimens.
Six lots (data pooled) of the AcroPrep Advance filter plate media (orange)
were evaluated for biotinidase response and consistently preserved the
full measure of enzyme activity in the specimens. All six lots demonstrated
100% biotinidase response. The AcroPrep Advance filter plates provide
the consistency needed for critical diagnostic screening assays for
newborns. Data generated in conjunction with Astoria-Pacific International.
Related Literature
PN 33569 Diagnostic Determination of Biotinidase Activity
Using AcroPrep Advance Filter Plates Application Note
40
35
30
Ordering Information
25
20
15
10
5
0
AcroPrep
Advance
Biotinidase Plates
AcroPrep
Advance
Neonatal Plates
Competitor M
Plates
Evacuation times of different filter plates (n=2) were tested for use in the
SPOTCHECK◆ Biotinidase Microplate assay. Both AcroPrep Advance
filter plates showed decreased processing time compared to Competitor
M allowing for increased throughput, especially on automated instrument
platforms. Data generated in conjunction with Astoria-Pacific International.
The AcroPrep Advance filter plate is a critical component in
the success of the biotinidase assay. Filter plates from another
manufacturer have been found to decrease the biotinidase
enzyme activity simply by being in contact with the specimens.
This effect varies from lot to lot and can lead to an increased
number of false positives (Figure 2). The resultant effect of false
positive results causes an emotional burden on families, a labor
burden on the laboratories, and a financial burden on the testing
and follow-up process.
AcroPrep Advance Filter Plates
Part Number
Description
8060*
For biotinidase assays
Pkg
10/pkg
8079*
10/pkg
For neonatal screening
*Available in Bulk Packs.
Complementary Products
Vacuum Manifold and Accessories
Part Number
Description
5017
Multi-well plate vacuum manifold
Pkg
1/pkg
5015
350 µL receiver plate spacer block
1/pkg
5016
Replacement accessory kit
(includes O-ring, gasket and allen wrench)
1/pkg
5028
Waste drain adaptor retainer
1/pkg
13157
Vacuum/pressure pump,
115 V, 60 Hz, single phase
1/pkg
Visit us on the Web at www.pall.com/oem
E-mail us at LabCustomerSupport@pall.com
Pall Life Sciences
600 South Wagner Road
Ann Arbor, MI 48103-9019 USA
1.800.521.1520 USA and Canada
(+)800.PALL.LIFE Outside USA and Canada
1.734.665.0651 phone
1.734.913.6114 fax
International Offices
Pall Corporation has offices and plants throughout the world in locations such as: Argentina, Australia, Austria,
Belgium, Brazil, Canada, China, France, Germany, India, Indonesia, Ireland, Italy, Japan, Korea, Malaysia, Mexico,
the Netherlands, New Zealand, Norway, Poland, Puerto Rico, Russia, Singapore, South Africa, Spain, Sweden,
Switzerland, Taiwan, Thailand, the United Kingdom, the United States and Venezuela. Distributors in all major
industrial areas of the world.
The information provided in this literature was reviewed for accuracy at the time of publication. Product data may
be subject to change without notice. For current information consult your local Pall distributor or contact Pall directly.
© 2010, Pall Corporation. Pall,
, and AcroPrep are trademarks of Pall Corporation. ® indicates
a trademark registered in the United States. Filtration.Separation.Solution.SM is a service mark of Pall
Corporation. ◆SPOTCHECK is a trademark of Astoria-Pacific International.
02/10, 1K, AA GN10.3392
PN33568