AcroPrep™ Advance Filter Plates
for Multiplexing Assays
Superior bead recovery and low levels of false positives
ensure assay reproducibility
Smooth well wall – Provides efficient bead recovery, ensuring reproducible results lot after lot.
High performance membrane – Does not trap microspheres in the membrane matrix.
Low levels of false positives – Features Supor® membrane with low non-specific binding properties
that minimize non-specific reactivity by the microspheres.
Plate redesign – Results in faster and more uiniform filtration rates across the plate.
New outlet tip geometry – Minimizes sample leakage and loss during incubation steps so that acquisition
times are not effected.
Low non-specific binding – Intrinsic plate and membrane properties prevent target analytes from binding
and interfering with assay performance.
100% visual inspection – Ensures each well has proper membrane placement and is free from debris.
Automation compatible – Manufactured in accordance with SBS guidelines, allowing plates to be run in
manual, semi-automated, and automated processes.
Applications
Bead-based multiplexing assays
Flow cytometry
Figure 3
Pall Supor Membrane Reduces the Occurrence of False
Positive Results
Performance
Figure 1
Supor Membrane Does Not Trap Microspheres
and Allows Efficient Bead Recovery
B: Competitor M membrane
The consistent membrane structure and smooth surface morphology
of Pall’s Supor membrane provides efficient recovery of microspheres.
The fibrous surface structure of the Competitor M membrane entraps
microspheres, making recovery difficult. Luminex xMAP◆ microspheres
were coated with a BSA solution and then diluted in PBS with 0.1% BSA
to 50,000 beads per mL. Images taken following filtration.
Average Weeping Wells
1000
100
10
1
Lot 1
Lot 2
Microsphere Lots
Competitor M
Lot 3
Pall Supor
4
The serological immunoassays were performed with multiple lots of
xMAP microspheres in both the Pall Supor and Competitor M filter
plates. The results from these filter plates were read with one Luminex
LX100 Instrument. The responses represent the reactivity toward
microspheres without proteins coupled to them to maximize the
indications of false positive “non-specific” reactivity by the microspheres.
The results represent the average of four replicates for each of the three
“positive” and one “negative” samples tested. In all lots of microspheres
tested, the Pall Supor filter plates exhibited a marked reduction in nonspecific reactivity than competitive plates. Data generated in conjunction
with Luminex Corporation.
2
Ordering Information
0
AcroPrep Advance Filter Plates
Part Number
Description
8049*
For multiplex assays
Pkg
10/pkg
6008019
0.2 µm Supor membrane
100/pkg
6008029
0.45 µm Supor membrane
100/pkg
Figure 2
AcroPrep Advance Exhibits Minimal Weeping During Incubations
Aqueous Solution
6
1 hr.
Average Weeping Wells
MFI Responses
A: Pall Supor membrane
10000
2 hr.
3 hr.
Time
AcroPrep Advance
8 hr.
24 hr.
Competitor M
*Available in bulk packs
Detergent Solution
8
Complimentary Products
6
4
2
0
1 hr.
2 hr.
3 hr.
Time
AcroPrep Advance
8 hr.
Vacuum Manifold and Accessories
Part Number
Description
5017
Multi-well plate vacuum manifold
Pkg
1/pkg
5015
350 µL receiver plate spacer block
1/pkg
5016
Replacement accessory kit
(includes O-ring, gasket and allen wrench)
1/pkg
24 hr.
Competitor M
Filter plates were filled with 200 µL of the indicated solutions and incubated
over receiver plates for 24 hours. Receiver plates were checked for the
presence of fluid at 1, 2, 3, 8, and 24 hour intervals. Three plates of
each variety were evaluated and the averages calculated. Aqueous
solution consisted of PBS, 0.8% BSA, 0.4% VitB12. Detergent solution
consisted of PBS, 0.8% BSA, 0.4% VitB12, 0.5% NP40.
5028
Waste drain adaptor retainer
1/pkg
13157
Vacuum/pressure pump,
115 V, 60 Hz, single phase
1/pkg
Visit us on the Web at www.pall.com/oem
E-mail us at LabCustomerSupport@pall.com
Pall Life Sciences
600 South Wagner Road
Ann Arbor, MI 48103-9019 USA
1.800.521.1520 USA and Canada
(+)800.PALL.LIFE Outside USA and Canada
1.734.665.0651 phone
1.734.913.6114 fax
International Offices
Pall Corporation has offices and plants throughout the world in locations such as: Argentina, Australia, Austria,
Belgium, Brazil, Canada, China, France, Germany, India, Indonesia, Ireland, Italy, Japan, Korea, Malaysia, Mexico,
the Netherlands, New Zealand, Norway, Poland, Puerto Rico, Russia, Singapore, South Africa, Spain, Sweden,
Switzerland, Taiwan, Thailand, the United Kingdom, the United States and Venezuela. Distributors in all major
industrial areas of the world.
The information provided in this literature was reviewed for accuracy at the time of publication. Product data may be
subject to change without notice. For current information consult your local Pall distributor or contact Pall directly.
© 2010, Pall Corporation. Pall,
, AcroPrep and Supor are trademarks of Pall Corporation. ® indicates
a trademark registered in the United States. Filtration.Separation.Solution.SM is a service mark of Pall
Corporation. ◆xMAP and Luminex are trademarks of Luminex Corporation.
02/10, 1K, AA GN10.3394
PN33570