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24.2 cm / 9.5"
17.2 cm / 6.8"
4. Ordering Information
Description
Part Number
PRC Column 5x50 KANEKA KanCapA, 1 mL
PRC05X050KANCAPA
PRC Column 8x100 KANEKA KanCapA, 5 mL
PRC08X100KANCAPA
PRC Column 11.3x50 KANEKA KanCapA, 5 mL
PRC11.3X050KANCAPA
Instructions For Use
Visit us at www.pall.com/biopharm
E-mail us at biopharm@pall.com
Corporate Headquarters
Port Washington, NY, USA
+1 800 717 7255 toll free (USA)
+1 516 484 5400 phone
biopharm@pall.com e-mail
European Headquarters
Fribourg, Switzerland
+41 (0)26 350 53 00 phone
LifeSciences.EU@pall.com e-mail
Asia-Pacific Headquarters
Singapore
+65 6389 6500 phone
sgcustomerservice@pall.com e-mail
International Offices
Pall Corporation has offices and plants throughout the world in locations such as:
Argentina, Australia, Austria, Belgium, Brazil, Canada, China, France, Germany,
India, Indonesia, Ireland, Italy, Japan, Korea, Malaysia, Mexico, the Netherlands,
New Zealand, Norway, Poland, Puerto Rico, Russia, Singapore, South Africa,
Spain, Sweden, Switzerland, Taiwan, Thailand, the United Kingdom, the United
States, and Venezuela. Distributors in all major industrial areas of the world. To
locate the Pall office or distributor nearest you, visit www.pall.com/contact.
The information provided in this literature was reviewed for accuracy at the time
of publication. Product data may be subject to change without notice. For
current information consult your local Pall distributor or contact Pall directly.
© 2016, Pall Corporation. Pall,
and Cadence are trademarks of Pall
Corporation. Filtration.Separation.Solution. is a service mark of Pall
Corporation. ® indicates a trademark registered in the USA. u KANEKA
KanCapA is a trademark of KANEKA CORPORATION. ÄKTA is a trademark of
GE Healthcare. KANEKA KanCapA is manufactured by KANEKA CORPORATION.
3/16, GN16.9926
USD 3090a
USD 3090a
PRC Prepacked Columns 1 mL and 5 mL
With KANEKA KanCapAu Protein A
Affinity Chromatography Sorbent
24.2 cm / 9.5"
1. Description and Main Properties
17.2 cm / 6.8"
Pall PRC chromatography columns have been developed for fast selectivity and
performance screening of chromatography sorbents. They guarantee optimal
and consistent performance:
l 1 mL and 5 mL prepacked columns, ready- and easy-to-use. The 5 mL
columns are available in two different bed heights, refer to Table 1.
l Direct connections to commonly used laboratory chromatography systems,
e.g., ÄKTAu system (see Table 1). The 11.3 x 50 mm column is particularly
suitable for connection to Pall’s Cadence™ BioSMB PD continuous multicolumn chromatography system
l High packing efficiency
l Fully scalable: once screened, the sorbent can be operated in the same
conditions at either laboratory, pilot or production scale
2. Instructions for Use
Table 1
Main Properties and Recommended Operating Conditions
KANEKA KanCapA Sorbent
Volume & bed dimensions
Base matrix
Particle size range
Ligand
Coupling chemistry
Dynamic binding capacity(1)
Chemical stability
Working pH range(2)
CIP condition(2)
Operational flow rate
Residence time
Delivery condition(3)
Storage condition(3)
Column
Outer dimensions
Materials of construction
Connections(4)
Maximum operating pressure
KANEKA KanCapA sorbent is designed for the purification of biomolecules
containing an Fc region of immunoglobulin and its derivatives from biological
samples and cell culture media. It is composed of highly cross-linked cellulose
beads and KANEKA’s proprietary alkaline resistant Protein A ligand. The cellulose base matrix has superior non-specific binding characteristics. KANEKA
KanCapA sorbent allows high flow rates during affinity purification of IgG at
development, pilot and commercial scale.
Benefits:
l Alkaline stability and long life cycle
l Mild pH elution
l High binding capacity
l High flow rate operation and easy scale up from pilot to process
An example of chromatographic conditions is detailed below. The optimal
conditions depend on the characteristics of feed material.
1 mL (5 mm I.D. x 50 mm)
5 mL (8 mm I.D. x 100 mm and 11.3 mm I.D. x 50 mm)
Highly cross-linked cellulose
65~85 μm
Recombinant Protein A (alkaline resistant)
Reductive amination
≥35 mg human polyclonal IgG/mL packed sorbent
Stable in solvents commonly used in affinity chromatography
pH 2~ pH 13
0.1~0.5 M sodium hydroxide, 6~8 M urea, 6 M guanidine hydrochloride
Up to 500 cm/hr (bed height: 20~25 cm)
≥3 min (4~6 min is recommended)
Slurry in 20% ethanol
Slurry in 20% ethanol at 1~10 °C
Table 2
Example of Chromatographic Conditions
Flow rate
Equilibration
Load
Wash
Elution
Strip
Neutralization
CIP
Re-equilibration
100-500 cm/hr(1) (a residence time of 4 to 6 minutes is recommended)
PBS, pH 7.4 (5 column volumes [CV])
IgG solution pH neutralized
PBS, pH 7.4 (5 CV)
Acetate buffer or citrate buffer, pH 3.5
1 M acetic acid (3 CV)
PBS, pH 7.4 (3 CV)
0.1 M sodium hydroxide(2) (15 minute contact time)
PBS, pH 7.4 (5 CV)
(1) The flow rate corresponding to a residence time of 4 to 6 minutes depends on the column length.
For columns of 5 cm height, 50-75 cm/hr flow rate gives 4-6 minutes of residence time. (2) 0.5 M
sodium hydroxide can also be used (refer to Table 1)
1 mL column: 10x128 mm
5 mL column: 10x164 mm and 16x124 mm
Body and end caps: Molded Polypropylene (PP)
17 μm frit: Polypropylene / Polyethylene (PP / PE)
Built-in 10-32 fittings
1 mL column: 20 bar g (290 psi g); 5 mL column: 30 bar g (435 psi g)
3. Thermal Stability and Storage
Temperature of use
Storage temperature
Storage solution between runs
(1) 5% dynamic binding capacity is determined by frontal analysis at 3 minute residence time.
(2) Alkaline conditions can be used for CIP but are unsuitable for long term exposure. (3) Prevent
from freezing (4) For HPLC/MPLC/ÄKTA systems: direct connection. For systems equipped with
2 to 30 °C
2 to 8 °C
20% ethanol
Column must never be frozen
Column is shipped at ambient temperature
¼-28 or M6 connections: use a standard 10-32 male/¼-28 or M6 female adaptor (not supplied).
2
USD 3090a
USD 3090a
www.pall.com/biopharm
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