5. Ordering Information
Description
Part Number
PRC Column 5x50 KANEKA KanCapA, 1 mL
PRC05X050KANCAPA
PRC Column 8x100 KANEKA KanCapA 5 mL
PRC08X100KANCAPA
Instructions For Use
USD 3090
PRC Prepacked Columns 1 mL and 5 mL
With KANEKA KanCapA Protein A Affinity
Chromatography Sorbent
u
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The information provided in this literature was reviewed for accuracy at the time of publication. Product data may be subject to
change without notice. For current information consult your local
Pall distributor or contact Pall directly.
© 2015, Pall Corporation.
and Pall are trademarks of Pall
Corporation. Filtration.Separation.Solution. is a service mark of
Pall Corporation. ® indicates a trademark registered in the USA.
u KANEKA KanCapA is a trademark of KANEKA CORPORATION.
ÄKTA is a trademark of GE Healthcare. KANEKA KanCapA is
manufactured by KANEKA CORPORATION.
9/15, GN15.9780
USD 3090
1. Description
Pall PRC chromatography columns have been developed for fast selectivity and
performance screening of chromatography sorbents. They guarantee optimal and
consistent performance:
• 5 mm I.D. x 50 mm bed height prepacked columns of 1 mL, and 8 mm I.D.x100 mm
bed height prepacked columns of 5 mL, ready- and easy-to-use
• Direct connections to commonly used laboratory chromatography systems, e.g.,
ÄKTAu systems (see Table 1)
• High packing efficiency
• Fully scalable: once screened, the sorbent can be operated in the same conditions
at either laboratory, pilot or production scale
KANEKA KanCapA sorbent is designed for the purification of biomolecules containing
an Fc region of immunoglobulin and its derivatives from biological samples and cell
culture media. It is composed of highly cross-linked cellulose beads and KANEKA’s
proprietary alkaline resistant Protein A ligand. The cellulose base matrix has superior
non-specific binding characteristics. KANEKA KanCapA sorbent allows high flow
rates during affinity purification of IgG at development, pilot and commercial scale.
Benefits:
• Alkaline stability and long life cycle
• Mild pH elution
• High binding capacity
• High flow rate operation and easy scale up from pilot to process
3. Instructions for Use
2. Main Properties
Table 1
Main Properties and Recommended Operating Conditions
An example of chromatographic conditions is detailed below. The optimal conditions
depend on the characteristics of feed material.
KANEKA KanCapA Sorbent
Table 2
Example of Chromatographic Conditions
Volume
1 mL (5 mm I.D. x 50 mm) or 5 mL ( 8 mm I.D. x 100 mm)
Base matrix
Highly cross-linked cellulose
Flow rate
100-500 cm/hr 1 (a residence time of 4 to 6 minutes is recommended)
Particle size range
65~85 μm
Equilibration
PBS, pH 7.4 (5 column volumes [CV])
Ligand
Recombinant Protein A (alkaline resistant)
Load
IgG solution pH neutralized
Coupling chemistry
Reductive amination
Dynamic binding capacity1
Wash
PBS, pH 7.4 (5 CV)
≥ 35 mg human polyclonal IgG/mL packed sorbent
Chemical stability
Stable in solvents commonly used in affinity chromatography
Elution
Acetate buffer or citrate buffer, pH 3.5
Working pH range2
pH 2~ pH 13
Strip
1 M acetic acid (3 CV)
CIP condition2
0.1~0.5 M sodium hydroxide, 6~8 M urea, 6 M guanidine hydrochloride
Neutralization
PBS, pH 7.4 (3 CV)
Operational flow rate
Up to 500 cm/hr (bed height: 20~25 cm)
CIP
0.1 M sodium hydroxide 2 (15 minute contact time)
Residence time
≥ 3 min (4~6 min is recommended)
Re-equilibration
PBS, pH 7.4 (5 CV)
Delivery condition3
Slurry in 20% ethanol
3
Storage condition
Slurry in 20% ethanol at 1~10 °C
Column
Outer dimensions
1 mL column: 10 x 128 mm ; 5 mL column: 10 x 164 mm
Materials of construction
Body and end caps: Molded PP (Polypropylene)
17 μm frit: PP / PE (Polypropylene / Polyethylene)
Connections4
Built-in 10-32 fittings
Maximum operating pressure
1 mL column: 20 bar g (290 psi g) ; 5 mL column: 30 bar g (435 psi g)
1
The flow rate corresponding to a residence time of 4 to 6 minutes depends on the column length.
For columns of 5 cm height, 50-75 cm/hr flow rate gives 4-6 minutes of residence time.
2
0.5 M sodium hydroxide can also be used (refer to Table 1)
4. Thermal Stability and Storage
Temperature of use
Storage temperature
2 to 30 °C
2 to 8 °C
Storage solution between runs
20% ethanol
1
5% dynamic binding capacity is determined by frontal analysis at 3 minute residence time.
2
Alkaline conditions can be used for CIP but are unsuitable for long term exposure. 3 Prevent
from freezing. 4 For HPLC/MPLC/ÄKTA systems: direct connection. For systems equipped with
¼-28 or M6 connections: use a standard 10-32 male/¼-28 or M6 female adaptor (not supplied).
2
Column must never be frozen
Column is shipped at ambient temperature
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3