University of Wisconsin-Stout
Student Dissemination Grant Proposal
Title: Participation at 2015 IFT Annual Meeting
(Research title: Production and Characterization of Lutein Nano-emulsion)
Submission Date: May 2nd, 2015
Project Period: July 11-July 14, 2015
To: Student Research Grant Office
From: Cindy Vang, undergraduate student of the Food Science and Technology Department
I. Summary
a. The purpose of this application is to apply for funds that would support the costs for
attending the 2015 IFT (Institute of Food Technologists) Annual Meeting & Food Expo
in Chicago, IL from J July 11-14, 2015. The objectives of attending the IFT are 1) to
present a research poster (title: Production and characterization of lutein nanoemulsion), 2) to botain feedback about my research from professional food science
group, and 3) to expand the knowledge of food science and professional networking
with people in academic and industry fields. I have a plan to attend scientific oral and
poster sessions and Food Expo to understand current food industry trends and to carry
out professional networking. If granted, the funds would be used for registration,
transportation, lodging and board.
b. Research Summary: Lutein, a lipid soluble bioactive compound, can play an important
role in eye health, including age-related macular degeneration and cataracts. Nanoemulsion technology incorporates lipid-soluble bioactive compounds, like lutein, in
emulsion and enables the lipid-soluble compounds in various food matrices, including
beverages. In addition, nano-emulsion can improve the functional stability of lutein,
which is sensitive to light and oxygen, and deteriorates easily during processing and
storage. The objectives of this study were to evaluate (1) the optimal conditions for
lutein nano-emulsion production, and (2) the physico-chemical properties of lutein
nano-emulsion. Primary emulsions were prepared with varying compositions of lutein,
water and surfactants including medium chain triglyceride (MCT), short-chain alcohols
(ethanol) and polyoxyethylene sorbitan monooleate (Tween 80). Primary emulsions
and water were mixed with 2.5:97.5 (w/w) and then nano-emulsions were produced
using the microfluidizer under high-pressure (25,000 psi) and 3 processing cycle
conditions added with 0.01% butylated hydroxyanisole and 0.05% sodium azide to
prevent oxidation and deterioration. Emulsion stability (particle size distribution and
zeta potential), droplet size and lutein contents of nano-emulsion were determined
using particle size analyzer, zeta potential analyzer, transmission electron microscopy,
and high performance liquid chromatography, respectively. Results showed that the
most stable primary emulsion was produced when lutein, MCT, Tween 80, ethanol and
water ratio was 0.1:35.2:5.9:11.8:47.0 (w/w). Lutein nano-emulsion appeared semitransparent with light yellow color and high fluidity. The average particle size of lutein
nano-emulsion was 65.8 nm and 77.9 nm at 0 and 7 days of storage, respectively. The
zeta potential was -12.45±1.69 and -11.44±2.70 at 0 and 7 days of storage, respectively.
In conclusion, lutein nano-emulsion was relatively stable during the 7-day storage and
has a high potential to apply in cold-beverage production. However, further study is
needed to improve the storage stability of lutein nano-emulsion to be used in beverages
that require longer storage time.
c. My Role: As an undergraduate student in Food Science and Technology program, I have
supported to supervisor (Dr. Eun Joo Lee). I have contributed and participated in the
project when the Dr. Lee has conducted the experimental design, searched reference
papers, analyzed data, and reported the results. Dr. Lee and I have submitted 2015
Student Research Grant proposal and then successfully granted. I made a research
poster using research results and then presented in 2015 UW-Stout Research Day
(April 29, 2015). The research abstract was submitted to national level conference
(2015 Institute of Food Technologist (IFT) Annual conference) and it was selected. So I
plan to present at the IFT annual meeting (July 11-14, 2015, Chicago, IL) as a main
presenter.
II. Mode of Dissemination
a. This research project will be disseminated at the 2015 Institute of Food Technologists
(IFT) Annual Meeting in Chicago, IL from July 11-14 as a poster presentation. IFT is the
largest national annual event for food scientists that bring respected food professionals
from around the world, including trendsetters from productive development to seniorlevel executives from the world’s top food companies. It is also expected to have 23,000
attendees. Each year the annual meeting invites researchers to share their research in a
poster presentation. My research abstract was submitted and was accepted to present
my poster (see attached conformation).
b. Presenting a research poster at the 2015 IFT Annual Meeting is significant due to the
large number of attendees. This will allow me to connect with professionals and receive
feedback and ideas while sharing my research.
c. I will be presenting my research as a poster presentation. This representation of my
research will be appropriate to explain best while referring to figures, tables, and
charts.
III. Purpose/Need for Dissemination
a. The significance of my research explores the different possibilities of creative product
development ideas that could potentially benefit human health and the field in nanotechnology. Use of skills and knowledge learned from this research project will be
beneficial for other food applications. Also, as an undergraduate at in the Food Science
program, building a reputation by presenting at a national-level conference will be
valuable for my academic career and my college, UW-Stout.
IV. Dissemination Objectives
a.
The objectives of this dissemination are as follows:
1. To present a research poster (title: Production and characterization of lutein nanoemulsion)
2. To obtain feedback about my research from professional food science group
3. To expand the knowledge of food science and professional networking with people
in academic and industry fields
V. Budget
SRG Request
Supplemental
Services and Supplies
Travel (via FOXWORLD
travel ***mandatory
travel agency)
Two night hotel stay
2 x $149.00 +tax= $346.84
Conference Registration
$125
Personal Vehicle Cost
308.8 miles x $0.32= $98.82
TOTAL
$540.66
a. Budget Justification: The cost to attend the 2015 IFT conference and Food Expo is total
$630.66. The IFT Annual Meeting is July 11-14 in Chicago, IL. I will be attending only
three days out of the four days so a two night stay in a hotel is required. A price of $149
for a hotel is very reasonable for hotel prices in Chicago and near the meeting.
Conference registration is $125 for students and entrance is required for my poster
presentation. I will take my personal vehicle to the conference since I will be located in
Verona, WI when the conference happens. A round trip, from Verona, WI to Chicago, IL
and back, will be a total of 308.8 miles ($0.32 per mile).
VI. References
Attwood, D., Mallon, C., Ktistis, G., & Taylor, C. J. (1992). A study on factors influencing the
droplet size in nonionic oil-in-water microemulsions. International Journal of
Pharmaceutics, 88, 417–422.
Davis B, Markey E, Busch A, Busch W. (2007). Determination of capsaicinoids in habanero
peppers by chemometric analysis of UV spectral data. J. Agr. Food Chem. 55: 5925-5933.
McClements, D. J., Decker, E. A., & Weiss, J. (2007). Emulsion-based delivery systems for
lipophilic bioactive components. Journal of Food Science, 72(8), R109–R124.
Yu W, Tabosa DE, Barrat ES, Fessi G, Devissaguet JP, Puisieux F. (1993). A novel approach to
the preparation of injectable emulsions by spontaneous emulsification process. Int. J.
Pharm. 89: 139-146.