Research Topics in Renewable Energy for 2016 Dosent / Lecturer: (Title, Initials & Surname) Prof KG Clarke Fakulteit / Faculty: Engineering Email: kclarke@sun.ac.za Tel: 021 808 4421 Departement / Department: Process Engineering Navorsingsarea / Area of Research: Bioprocess Engineering, Biotechnology Algemene beskrywing van navorsingsveld: (nie meer as 2 paragrawe nie): General description of research area (only 2 paragraphs): Heightened interest in diverting energy production from fossil fuels has targeted energy from biofuels (typically ethanol), obtained from renewable plant material, as a solution. Lignocellulosic plant material in particular has rapidly gained popularity in consideration of global food security with lignocellulosic hydrolysates, comprising mainly glucose and xylose, providing the feedstock of choice. The bioconversion of these hydrolysates to ethanol is recognised as a critical step in the lignocellulose to ethanol process and requires an efficient biocatalyst which is able to ferment C5 as well as C6 sugars. The bioconversion of C5/C6 sugars to ethanol using yeasts has been well documented. However, considerably less is known regarding the capabilities of other organisms to ferment these sugar mixtures. Yet the anaerobe Zymomonas mobilis has shown remarkably efficient glucose and xylose fermenting capabilities. The ultimate goal is to define a process for the bacterial bioconversion of C5 and C6 sugars to ethanol in terms of optimum bacterial growth, sugar utilisation and ethanol production kinetics. To date we have achieved a substantial (more than 5-fold increase) in ethanol production with Z. mobilis over its yeast counterparts, even those used in commercial production. This project will be directed towards the bioconversion of C5 and C6 sugars to ethanol by Z. mobilis under defined and controlled conditions in highly instrumented laboratory scale bioreactors with special emphasis on continuous operation strategies. Specifically, the potential of Z. mobilis for lignocellulosic hydrolysate bioconversion will be optimised through process intensification with a view to establishing this organism as a leading biocatalyst in the biofuel context. Lys van onderwerpe/List of topics: (Merk graad met X/Mark degree with X) Process intensification of biofuel from bacterial cultures M.Eng. M.Eng. Ph.D. (Structured) (Research) x Spesifieke voorvereistes / Specific requirements: BEng or BScEng in Process, Chemical, Biological, Biochemical or Biosystems Engineering or BSc Honours in Biotechnology, Biochemistry or Microbiology Befondsing beskikbaar / Funding available: Capital and running expenses available. Bursary required.