Application Note Preparation of a Glycerol Solution with the Pall Jet Mixer

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Application Note
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Preparation of a Glycerol Solution with the
Pall® Jet Mixer
Mixing system: Pall Jet Mixer
Mixing biocontainer: 200 L custom mixer biocontainer
Application mixing type: Liquid-liquid
The Pall Jet Mixer is one of the lightest and most compact single-use mixing systems. It
consists of a cubical shaped single-use biocontainer with a magnetically impelled turbine,
secured to the bottom of the biocontainer, working as a centrifugal pump.
The established 3D recirculation loop eliminates dead zones and ensures efficient and
fast mixing.
Introduction
Liquid-liquid homogenization is a common requirement in biopharmaceutical processing,
including after storage. Furthermore, a cubical biocontainer shape is generally considered
to be preferable from a storage efficiency (footprint) viewpoint. However, performing mixing
in a cubical biocontainer can be difficult; some mixing technologies can be prone to uneven
distribution within the mixing biocontainer, or can even introduce unwanted particulate
contamination. The Jet Mixer impeller was developed to overcome these challenges.
In this experiment, a Jet Mixer was used to prepare a 10% glycerol solution. The objective
was to compare the mixing efficiency when the dense solute was added to a mixed solvent
versus a quiescent solvent.
Experimental
A 200 L custom mixer biocontainer was filled with 180 L of DI water, and the Jet Mixer
impeller mixing speed set to 1000 rpm. After 5 minutes, 20 kg of glycerol was added to
the mixing biocontainer in one of the top corners. Solution homogeneity was monitored
via refractive index readings on samples taken from the top and the bottom of the mixing
biocontainer.
The experiment was repeated with the Jet Mixer impeller set to a speed of 0 rpm during
the glycerol addition, which was done with a pipette to deposit the denser glycerol on the
bottom of the mixing biocontainer. Once the glycerol had been added, the impeller speed
was ramped up to 1000 rpm over 60 seconds. This was intended to represent a worst
case scenario.
Results
Figures 1 and 2 show the solution homogeneity in the biocontainer during mixing. Even under the most unfavorable
conditions, a very high degree of homogeneity was attained within 13 minutes.
Figure 1
Mixing of 20 kg glycerol in 180 L water FROM REGIME
Figure 2
Mixing of 20 kg glycerol in 180 L water FROM REST
1.360
1.360
Bottom
Top
Bottom
Top
1.355
Refraction Index
Refraction Index
1.355
1.350
1.345
1.340
1.350
1.345
1.340
1.335
1.335
1.330
1.330
0
10
20
30
40
Time / Minutes
50
60
70
0
10
20
30
40
Time / Minutes
50
60
70
Conclusions
The Pall Jet Mixer is well suited for liquid-liquid applications where a high degree of final homogeneity is important.
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