Protocol for the Preparation of Zebrafish Embryos for LLO Analysis

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Protocol for the Preparation of Zebrafish Embryos for LLO Analysis
1) 150-200 4-7 day old embryos are collected in an 1.8 mL eppendorf tube
and all excess water is removed (if using 4 day old or younger embryos,
yolks are removed prior to preparation)
2) 1 mL of methanol at room temperature is added to the tube and followed
by brief sonication (low setting, 3 x 5 sec pulses).
3) This mixture is then transferred into a 15-mL conical tube and an
additional 10-12 mL of methanol is added.
4) Following thorough vortexing, the tube is sealed and sent at room
temperature.
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