ANIMAL BIOLOGY (1604) LABORATORY Week of 14 September Phylum Platyhelminthes & Phylum Nematoda Read pages 93-100, 107-110 in your lab manual before coming to lab. Objectives: • Recognize and distinguish between the three platyhelminthes classes. • Understand characteristics associated with different flatworm lifestyles. • Recognize the basic characteristics of nematodes. Phylum Platyhelminthes • Aquatic or parasitic flatworms • Soft-bodied, bilaterally symmetric • Triploblastic tissue (acoelomate) Class Turbellaria (planarians) • Mostly free-living, carnivorous, aquatic forms • Ciliated epidermis Planaria: whole mount slide (Fig. 7.2 & Atlas Fig. 6.2) Identify the following structures: • Head • Eye spots • Auricles • Gastrovascular cavity • Pharynx • Mouth Planaria: cross-section through pharyngeal region slide (Fig. 7.3 & Atlas Fig. 6.3) Identify the following structures: • Epidermis • Mesoderm • Gastrodermis • Pharynx • Pharyngeal cavity • Gastrovascular cavity Class Trematoda (flukes) • Parasitic with wide-range of invertebrate and vertebrate hosts • Suckers for attachment to host Fasciola: whole mount slide (Fig. 7.4 & Atlas Fig. 6.5) Identify the following structures and label the image below: • Oral sucker • Ventral sucker • Testes Below is an image of Clonorchis sinensis (Fig. 7.4 & Atlas Fig. 6.8-6.12) Identify the following structures and label the image below: • Oral sucker • Ventral sucker • Pharynx • Testes • Uterus • Gastrovascular cavity • Excretory pore What anatomical features of trematodes suggest adaptation to a parasitic lifestyle? Class Cestoda (tapeworms) • Parasitic in most vertebrate hosts • Complex lifecycle with intermediate and multiple hosts Taenia: composite slide (Fig. 7.5) Identify the following structures: • Scolex • Hooks • Suckers Lab Manual: Lab Atlas: • Neck • Immature proglottids • Mature proglottids • Gravid proglottids Fig. 7.1-7.5 Fig. 6.1-6.18 Review Questions All Questions Questions 4-6 pg. 100 pg. 106 Exercise 8-1: Nematode Anatomy Ascaris Dissection (see instructions: pg. 108-110; Fig. 8.1 & Atlas Fig. 9.2-9.8) Phylum Nematoda • Pseudocoelom with complete digestive tract • Usually dioecious • Free-living and parasitic members Ascaris: Identify the following structures: • Mouth • Pharynx • Pseudocoelom • Anus • Females • Males Exercise: Live cultures Obtain a sample from one of the three numbered containers at the front of the room. Identify the sample as containing cnidarians, turbellarians or nematodes and record your answer in the chart below, including a brief description of how the organisms move. Identify the organisms and observe movement for the other two containers. *Have your TA check your identifications. Container 1: Container 2: Container 3: Review Questions Questions 1-4 All Questions pg. 114 pg. 110 *Read pages 115-132 in your lab manual before coming to lab next week