DIELECTRIC PROPERTIES OF HONEYDEW MELONS AND CORRELATION WITH QUALITY Wen-chuan Guo1 , Stuart O. Nelson2, Samir Trabelsi2, and Stanley J. Kays3 Northwest Agriculture and Forestry University, College of Mechanical and Electronic Engineering, Yangling, China 712100 2 U. S. Department of Agriculture, Agricultural Research Service, Russell Research Center, P. O. Box 5677, Athens, Georgia 30604-5677, USA 3 The University of Georgia, Department of Horticulture, Athens, Georgia 30602, USA 1 Dielectric properties of three honeydew melon cultivars, grown and harvested to provide a range of maturities, were measured with an open-ended coaxial-line probe and impedance analyzer over the frequency range from 10 MHz to 1.8 GHz. Probe measurements were made on the external surface of the melons and also on tissue samples from the edible internal tissue. Moisture content and soluble solids content (SSC) were measured for internal tissue samples, and SSC (sweetness) was used as the quality factor for correlation with the dielectric properties. Individual dielectric constant and loss factor correlations with SSC were low, but a high correlation was obtained between the SSC and permittivity from a complex-plane plot of dielectric constant and loss factor, each divided by SSC, for both the external surface and internal tissue measurements. However, SSC prediction from the dielectric properties by these relationships was not as high as expected. Permittivity data (dielectric constant and loss factor) for the melons are presented graphically to show their relationships with frequency for external surface and internal tissue measurements. A dielectric relaxation for the external surface measurements, which is attributable to bound water and Maxwell-Wagner relaxations, is also illustrated. Coefficients of determination for complex-plane plots, moisture content and SSC relationship, and penetration depth are also shown graphically. Further studies are needed for determining the practicality of sensing melon quality from the dielectric properties. Submission Date: 10 October 2006 Acceptance Date: 19 June 2007 Publication Date: 15 August 2007 INTRODUCTION Rapid and reliable nondestructive determination of quality and related characteristics of agricultural products can be helpful to producers, handlers and processors, those marketing the produce, and consumers. Visible and physical characteristics of many Keywords: Dielectric properties, melons, quality, soluble solids, permittivity, dielectric relaxation 41-2-44 fresh fruits and vegetables are available for correlation with quality, and some of these, such as color, size, weight, density, elasticity, and firmness are used in automatic sorting of some produce into different categories for the market [Guo et al., 2001], [Kays and Paull, 2004]. For the honeydew melon, however, no useful characteristics of this type have been found for reliable correlation with quality. Electrical properties of fruits and vegetables known as dielectric properties have Journal of Microwave Power & Electromagnetic Energy Vol. 41, No. 2, 2007 been investigated in several studies. Seaman and Seals [1991] found that the values of microwave dielectric properties were different for the surface and internal tissues of fruits. Microwave dielectric properties of fresh fruits and vegetables have been considered for potential use in nondestructive sensing of quality factors, such as maturity in peaches and chilling injury in sweetpotatoes [Nelson, 1980]. Possible permittivity-based maturity indices were identified for tree-ripened peaches [Nelson et al., 1995], but much further research was needed to assess the practicality of such techniques. Other measurements provided dielectric properties data for fresh fruits and vegetables over the frequency range from 200 MHz to 20 GHz [Nelson et al., 1994] and over the range from 10 MHz to 1.8 GHz [Nelson, 2003]. Data for tree-ripened peaches over the 200-MHz to 20-GHz range indicated an interest in such data at frequencies below 200 MHz [Nelson et al., 1995]. Therefore, the frequency range of 10 MHz to 1.8 GHz was selected for dielectric spectroscopy measurements of honeydew melon tissue to learn whether differences in the dielectric properties might be correlated with quality [Nelson et al., 2006]. These same data were studied in relation to dielectric relaxation in complex systems and subjected to advanced mathematical analysis and curve fitting [Nigmatullin et al., 2006]. The best criterion for melon quality is the content of soluble solids [Dull et al., 1990], which are mostly sugars (i.e., 80-85%), and therefore a measure of sweetness. This requires the extraction of tissue samples from the melons and measurement of expressed juice with a refractometer, which has been calibrated to indicate percentage, by weight, of soluble solids. In the studies on honeydew melons, although correlations with soluble solids content (SSC) were not judged sufficiently high, a high correlation was found for a complex-planeplot of the dielectric constant and loss factor, each divided by SSC. In subsequent research, honeydew melons were grown and harvested with a range of maturities, and dielectric properties were measured for both the surface and internal tissues along with SSC. Results of the new studies are reported in this paper. MATERIALS AND METHODS Honeydew Melons Three cultivars of honeydew melons (Cucumis melo L. Inodorus group, ‘Early Dew’, ‘Honey Brew’, and ‘Rocio’) were grown at the University of Georgia Horticulture farm, Watkinsville, Georgia, USA, in a Cecil clay loam soil using standard melon culture methods [Boyham et al., 2006]. Plantings were made on June 8, 13, and 19, 2006, with seeds spaced 1 × 3 m. Fungicide applications were alternated between Bravo and Pristine at two-week intervals. Fertilization was based upon soil analysis and culture recommendations [Boyham et al., 2006]. Fruit of varying stages of maturity was harvested over a 5-week period for analysis. Honeydew melons not measured on the harvest day were stored at 4°C from time of harvest until time for measurements, which was not more than a few days. Measurements were taken on fourteen, twenty, and ten melons of the ‘Early Dew’, ‘Honey Brew’, and ‘Rocio‘ cultivars, respectively. Dielectric Properties Measurements The electrical measurements necessary for dielectric properties determination were obtained with a Hewlett-Packard1 85070B openended coaxial-line probe, and a Hewlett-Packard 4291A Impedance/Material Analyzer [Nelson et Mention of company or trade names is for purpose of description only and does not imply endorsement by the U. S. Department of Agriculture. 1 International Microwave Power Institute 41-2-45 Figure 1. Surface measurement of honeydew melon dielectric properties with open-ended coaxial-line probe and impedance analyzer. al., 2006]. Permittivities (dielectric constants and loss factors) were calculated with Agilent Technologies 85070D Dielectric Probe Kit Software, modified for use with the HP 4291A Analyzer by Innovative Measurement Solutions, which provided permittivity values from the reflection coefficient of the material in contact with the active tip of the probe [Blackham and Pollard, 1997]. Settings were made to provide measurements at 51 frequencies on a logarithmic scale from 10 MHz to 1.8 GHz. The 4291A Analyzer was calibrated with an open, short, and matched load prior to the calibration of the openended coaxial-line probe with measurements on air, a short-circuit block, and glass-distilled water at 25 °C. A personal computer was used to control the system and record resulting data. measurements were made with the probe in firm contact with the surface of the melons in the equatorial region at four points about 90° apart around the perimeter of the melon. Melons were supported and maintained in firm contact with the probe with a laboratory jack as shown in Figure 1. Samples for measurements on the internal melon tissue were all taken from an equatorial slice, about 4 cm thick, cut with a sharp knife from the center of the melon perpendicular to the proximal-to-distal (stem to blossom) axis. From this slice, three cylindrical core samples were cut for the dielectric properties measurements as shown in Figure 2. Three more samples adjacent to the first samples were taken for moisture content determination. Moisture contents were determined by drying the triplicate samples in disposable 57-mm aluminum weighing dishes that were placed in a forced-air drying oven for 24 hours at 70 °C. Upon removal from the oven, weighing dishes with samples were cooled in a desiccator over anhydrous CaSO4 prior to reweighing for determination of moisture loss. Samples were cut from the equatorial melon slice with a cylindrical cutter [Nelson, 2003] that provided right circular cylindrical samples of 18.6-mm diameter and 3-to 4-cm length. All permittivity measurements were taken at 24 °C. Measurement procedures Melons were removed from 4-°C storage the night before measurements were scheduled and washed with tap water to remove any dust or soil material and permitted to equilibrate to 24 °C for the measurements. Initial permittivity 41-2-46 Figure 2. Equatorial slice of honeydew melon and location from which cylindrical tissue samples were cut. Journal of Microwave Power & Electromagnetic Energy Vol. 41, No. 2, 2007 of the sample. Thus, six series of permittivity measurements were made for each tissue sample at 51 frequencies from 10 MHz to 1.8 GHz to be averaged for each melon. The four external surface measurements were also averaged in a similar way. Upon completion of the internal tissue permittivity measurements, each sample was placed in a 1-oz. (30-ml) glass jar with screwon cap and held from a few minutes to 3 hours for soluble solids content determination. The measurements for soluble solids were determined with an Atago Pallete Series Model PR101α digital refractometer. Melon tissue samples were 7 placed in a garlic press with cheesecloth patches Figure 3. Internal tissue sample measurement of several layers to strain the juice expelled for with open-ended coaxial-line probe. the refractometer measurements. Five readings were taken for each sample, and the composite Samples wereofsupported as shown in Figure 3 on than those of the surface measurements at the higher dielectric constants the internal tissues are greater soluble solids content determination for each an aluminum platform that was raised to bring melon was a mean of 15 readings. the sample into fiofrmthecontact theare openfrequencies. Loss factors internalwith tissues much higher than those of the surface measurements at the lower ended coaxial-line probe for the permittivity frequencies, but they are[Nelson, quite similar at the higher measurements 2003]. When thatend of the frequency range, thus illustrating the dominant measurement was completed, the sample was influence of ionic conduction theend, lowerand frequencies and the dipolar losses at the higher frequencies. removed, turned endatfor reinserted for permittivity measurements on the other end 160 140 Permittivity 120 Dielectric constant Loss factor 100 80 60 40 20 0 107 108 109 Frequency, Hz Figure 44 melons. melons. Figure4.4.Mean Meanpermittivity permittivityvalues valuesfor forexternal externalsurface surface measurements measurements on on 44 International Microwave Power Institute 600 41-2-47 10 10 10 Frequency, Hz Figure 4. Mean permittivity values for external surface measurements on 44 melons. 600 500 Permittivity 400 Dielectric constant Loss factor 300 200 100 0 107 108 109 Frequency, Hz Figure 5. Figure Mean permittivity values for values internalfor tissue measurements on 44 melonsonwith mean moisture content of 5. Mean permittivity internal tissue measurements 44 melons with mean 91.3%, wet basis,moisture and meancontent solubleofsolids content of 8.4% 91.3%, wet basis, and mean soluble solids content of 8.4%. RESULTS AND DISCUSSION Frequency Dependence of Permittivities of Honeydew Melons Mean values for all the permittivity measurements on the 44 honeydew melons are shown in Figures 4 and 5. Permittivity values and their frequency dependence are quite different for the external surface and internal tissue measurements. Although the dielectric constants behave similarly with frequency, those for the surface measurements are somewhat greater than the internal tissue measurements at the lower frequencies, and the dielectric constants of the internal tissues are greater than those of the surface measurements at the higher frequencies. Loss factors of the internal tissues are much higher than those of the surface measurements at the lower frequencies, but they are quite similar at the higher end of the frequency range, thus illustrating the dominant influence of ionic conduction at the lower frequencies and the 41-2-48 dipolar losses at the higher frequencies. When the data from Figure 5 are observed in a log-log plot, as shown in Figure 6, the linear relationship between the log of the dielectric loss factor and the log of frequency is evident at frequencies below 600 MHz, which is indicative of the dominance of ionic conductivity at the lower frequencies. Similar results were noted for the dielectric loss factors from tissue measurements on other fruits and some vegetables [Nelson, 2005]. However, data for the external surface permittivity measurements (Figure 4), reveal an overriding dielectric relaxation behavior. Judging from the frequency range for this relaxation, the behavior is likely explained by bound water and Maxwell-Wagner relaxations [Hasted, 1973]. Correlation Between Permittivity and Soluble Solids Content To study the correlations of the dielectric properties with the chosen quality factor, soluble Journal of Microwave Power & Electromagnetic Energy Vol. 41, No. 2, 2007 [Hasted, 1973]. . 1000 Permittivity Dielectric constant Loss factor 100 10 107 108 109 Frequency, Hz Figure 6. Log-log plot of the permittivity of internal tissues of honeydew melons. Figure 6. Log-log plot of the permittivity of internal tissues of honeydew melons. solids content (SSC), linear regressions of the were obtained at 1.8 GHz. dielectric constant ε ′ , loss factor ε ′′ and loss The high correlation shown in Figures 8 Correlation Between Permittivity and Soluble Solids Content tangent ( tan δ = ε ′′ / ε ′ ) on SSC were calculated and 9 leads one to expect that the dielectric To study correlations of the dielectric with the chosen quality soluble solidsthe content (SSC), for the the 51 frequencies from 10 MHzproperties to 1.8 GHz. properties might factor, be used to predict soluble Coefficients of determination, r2, for the dielectric solids content [Nelson et al., 2006]. Referring to linear constant regressions thefactor dielectric constant � � surface , loss factorFigure � �� and tangent ( tan � � � �� /of � the ) onstraight SSC were andof loss from the external 8 orloss Figure 9, the equation measurements were very low, less than 0.25 at line can be written as calculated for the 51 frequencies from 10 MHz to 1.8 GHz. Coefficients of determination, r2, for the dielectric all frequencies. Better correlations were found ε′ forand the loss dielectric of the internal ε ′′ low, less constant factor properties from the external surface tissue measurements were very (1) = a f −than k 0.25 at all frequencies. measurements, with highest r2 values at 1.8 GHz. s s ε ′ and 0.752 r2 values fordielectric Better Those correlations were were found0.675 for the properties of the internal tissue measurements, with highest r2 for tan δ . The regression of tan δ on SSC is where s represents soluble solids content, a f illustrated in Figure 7. In previous studies on honeydew melons is the slope of the line, and k is the ε ′ / s -axis intercept. Values for the regression constants a f [Nelson et al., 2006] a high correlation was found between the permittivity and SSC in a and k are provided by the regression calculation. complex-plane plot of ε ′′ / SSC and ε ′ / SSC at Therefore, solving (1) for s provides SSC in terms of the dielectric properties as 1.8 GHz such as those shown in Figures 8 and 9. Figure 8 shows results for the external surface a f ε ′ − ε ′′ measurements at 1.8 GHz on mean values for the s = (2) 44 honeydew melons in the work reported here, af k and Figure 9 shows results for the internal tissue measurements. Linear regressions of ε ′′ / SSC on ε ′ / SSC were calculated at all 51 frequencies For the melon data of Figure 8, a f = 0.2104 and k = 0.2239. For that of Figure 9, a f = 0.1198 for both the external surface and internal tissue and k = −3.4891. measurements. Results are shown in Figure 10 where the best coefficients of determination International Microwave Power Institute 41-2-49 0.21 0.20 Loss tangent 0.19 0.18 0.17 0.16 0.15 0.14 0.13 4 6 8 10 12 14 Soluble solids content, % Figure 7. Linear regression of loss tangent on soluble solids content for internal tissues of honeydew melons at 1.8 GHz, r2 = 0.752. 10 2.4 2.2 2.0 1.8 �''/SSC 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 2 4 6 8 10 12 �'/SSC Figure 8. Complex-plane plot for 1.8-GHz permittivity from external surface measurements Figure 8. Complex-plane plot for permittivity from surface measurements divided by soluble divided by 1.8-GHz soluble solids content forexternal honeydew melons, r2 = 0.954. solids content for honeydew melons, r2 = 0.954. 2.4 41-2-50 2.2 2.0 Journal of Microwave Power & Electromagnetic Energy Vol. 41, No. 2, 2007 �'/SSC Figure 8. Complex-plane plot for 1.8-GHz permittivity from external surface measurements divided by soluble solids content for honeydew melons, r2 = 0.954. 2.4 2.2 2.0 �''/SSC 1.8 1.6 1.4 1.2 1.0 0.8 4 6 8 10 12 14 16 �'/SSC Figure 9. Complex-plane plot for 1.8-GHz permittivity from 11 internal tissue measurements divided by soluble solids 953. for 1.8-GHz permittivity from internal tissue measurements content for honeydew melons, r2 = 0. plot Figure 9. Complex-plane divided by soluble solids content for honeydew melons, r2 = 0. 953. 1.0 Coefficient of determination 0.9 0.8 0.7 0.6 External surface measurements Internal tissue measurements 0.5 0.4 0.3 0.2 107 108 109 Frequency, Hz Fig.10. Coefficients of determination for linear regressions of � ' ' / SSC on � ' / SSC for 44 honeydew melons Figure 10. Coefficients of determination for linear regressions of ε ′′ / SSC on ε ′ / SSC for 44 honeydew melons. The high correlation shown in Figures 8 and 9 leads one to expect that the dielectric properties might be used to Microwave Power Institute 41-2-51 predict theInternational soluble solids content [Nelson et al., 2006]. Referring to Figure 8 or Figure 9, the equation of the straight line can be written as use the relationship illustrated in Figures 8 for more reliable prediction of SSC lies in the fact that neither � � nor � �� were individually well correlated with SSC. 14 Predicted SSC, % 12 10 8 6 4 2 2 4 6 8 10 12 14 Measured SSC,% Fig. 11 Predicted SSC values from permittivity measurements on internal tissues of 44 honeydew melons, r2 = 0. 748. Figure 11. Predicted SSC values from permittivity measurements on internal tissues of 44 honeydew melons, r2 = 0. 748. PenetrationDepth Soluble content, s, was calculated Since thesolids attenuation of electromagnetic waves in the melon tissue is important in attempting quality, the 2 to sense ε ′′ from Equation (2) for the 44 melons used in 2π ε ′ = attenuation 1is +described 1 the −by nepers/m penetration depth at the different frequencies is of interest. αThe attenuation (3) this study, both for the external surface measureλ0 2 ε′ mentsconstant, and the� internal tissue measurements, , in terms of the dielectric properties of for the material as follows: comparison with measured SSC. Predicted SSC where λ0 is the free-space wavelength. For did not compare very well for the exterior surattenuation in decibels, dB/cm = 0.08686 face measurements, r2 = 0.015. For the internal 2 � � � 2� � � �� Therefore, the attenuation to � 1 � �� × ��nepers/m. (3) � 1� nepers/m � better, tissue measurements, predicted SSC �was � � expected 2 � � � � � 0 be in honeydew melon tissue was � � r2 = 0.748 as illustrated in Figure 11. Thus the calculated with the measured dielectric constant opportunity for a nondestructive determination dB/cm = 0.08686 � nepers/m.from Therefore, the where �0 is the free-space wavelength. For attenuation in decibels, and loss factor data obtained the external from external surface measurements does not apsurface and internal tissue measurements pear promising. explanation for the inability attenuation to The be expected in honeydew melon tissue was calculated with the measured dielectric constant and loss to use the relationship illustrated in Figures 8 for all frequencies in the range used in these factor data obtained from the external surface and internal tissue measurements for all frequencies in the range used for more reliable prediction of SSC lies in the measurements. The depth of penetration, d p=1/(2α), based on the average measured fact that neither ε ′ nor ε ′′ were individually well permittivities is shown in Figure 12. At the lower correlated with SSC. frequencies, the depth of penetration is much greater, based on the permittivity values obtained Penetration Depth by the external surface probe measurements, than it is in the internal melon tissue. At microwave Since the attenuation of electromagnetic waves in the melon tissue is important in attempting to frequencies, the indicated depth of penetration is nearly the same for internal tissue and that near sense quality, the penetration depth at the different frequencies is of interest. The attenuation the surface of the melons. is described by the attenuation constant, α, in terms of the dielectric properties of the material as follows: Correlation Between Moisture Content and SSC Correlations of dielectric constant and loss factor with moisture content (wet basis) were 41-2-52 Journal of Microwave Power & Electromagnetic Energy Vol. 41, No. 2, 2007 melons. Penetration depth, cm 100 External surface measurements Internal tissue measurements 10 1 107 108 109 Frequency, Hz 14 based on permittivities calculated from external surface Figure 12. Dependence of penetration depth on frequency, Figure 12. Dependence of penetration depth on frequency, based on permittivities calculated probe measurements and probe measurements on internal tissues. from external surface probe measurements and probe measurements on internal tissues. 96 Moisture content, % 94 Correlation Between Moisture Content and SSC 92 Correlations of dielectric constant and loss factor with moisture content (wet basis) were low, r2 less than 0.2. 90 However, SSC was highly correlated with tissue moisture content in honeydew melons [Nelson et al., 2006]. For the 44 honeydew melons in this study, SSC was highly correlated with moisture content as shown in Figure 13. 88 86 4 6 8 10 12 14 SSC, % Figure 13. Linear Linearregression regression of moisture content, on soluble solidss,content, s, formelons, honeydew Fig. 13 of moisture content, M, on M, soluble solids content, for honeydew M � 99.5 � 0.985s , r2=0.972 melons, M=99.5-0.985s, r2=0.972. low,CONCLUSIONS r2 less than 0.2. However, SSC was highly CONCLUSIONS correlated with tissue moisture content in honMeasurements of dielectric constant and loss factor with an open-ended coaxial-line probe and impedance analyzer eydew melons [Nelson et al., 2006]. For the 44 Measurements of dielectric constant and loss on external surfaces and internal tissue of three cultivars of honeydew melons provided new permittivity data over a honeydew melons in this study, SSC was highly factor with an open-ended coaxial-line probe correlated with moisture content as shown in impedance analyzer on external surfaces range of maturities at frequencies from 10 MHz to 1.8 GHz atand 24 �C. Both the dielectric constant and loss factor of and Figure 13. internal tissues of three cultivars of honeydew internal tissues decreased regularly with increasing frequency showing the dominance of ionic conduction at lower melons provided new permittivity data over a range of maturities from 10 MHz frequencies and dipolar losses at the higher frequencies. Surface measurements revealedata frequencies prominent and overriding dielectric relaxation at lower frequencies and the characteristic decline in dielectric constant value with increasing frequency. At all frequencies in the range studied, individual correlations of dielectric constant and loss factor with International Microwave Power Institute 41-2-53 soluble solids content (SSC) (sweetness) were low, but coefficients of determination of about 0.7 were observed for the loss factor and loss tangent of internal tissues at 1.8 GHz. However, the complex-plane plot of dielectric to 1.8 GHz at 24 °C. Both the dielectric constant and loss factor of internal tissues decreased regularly with increasing frequency showing the dominance of ionic conduction at lower frequencies and dipolar losses at the higher frequencies. Surface measurements revealed a prominent and overriding dielectric relaxation at lower frequencies and the characteristic decline in dielectric constant value with increasing frequency. At all frequencies in the range studied, individual correlations of dielectric constant and loss factor with soluble solids content (SSC) (sweetness) were low, but coefficients of determination of about 0.7 were observed for the loss factor and loss tangent of internal tissues at 1.8 GHz. However, the complex-plane plot of dielectric constant and loss factor, each divided by SSC, provided a high correlation, r2 = 0.95 for both the external surface and internal tissue measurements. Prediction of SSC values from these relationships between SSC and the dielectric properties, however, was low with a coefficient of determination, r2, of 0.015 for surface measurements and 0.75 for internal tissue measurements. Reliability of SSC predictions from the dielectric properties was low because of the poor individual correlations of dielectric properties with SSC. Penetration depths for electromagnetic fields in these frequencies ranged from about 2 cm at the highest frequencies to about 100 cm and 15 cm for the surface and internal tissues, respectively, at 10 MHz. Further studies are needed to determine whether practically useful techniques can be developed for reliable prediction of quality from dielectric properties of the melons. REFERENCES Blackham, D.V. and Pollard, R.D. 1997. An improved technique for permittivity measurements using a coaxial probe. IEEE Transactions on Instrumentation and Measurement 46(5): 1093-1099. 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