Histology Lab 1
Introduction and Epithelium
Jun Zhou（周俊）, Ph.D & M.D
School of Medicine,Zhejiang University
Note:
Please come lab on time.
Find your seat according your lab number.
 Your final score is composed of four parts
1) Attendance and picture drawing:10%
2) Quiz: 15% (each quiz 5%)
3) Final lab test: 25%
4) Final written examination: 50%
How to pass this course:
Final score>60 and Final written exam>50
Lab facilities:
 1 Light electron microscope with computer
 1 case of slides
 1 atlas
 http://m-learning.zju.edu.cn
1.Students share these facilities
2.Do not take them out the laboratory with you.
3.Put them where they belong to when lab is over.
4.Do not left any rubbish after you.
5.No food in the lab.
6.Lecture and lab slides online:
m-learning.zju.edu.cn 2015 Histology and Embryology
Contents of Lab 1:
1. Introduction
2. Epithelium
1)Simple squamous epi (small intestine,stomach)
2)Simple cuboidal epi.(thyroid)
3)Simple columnar epi.(small intestine)(drawing)
4)Pseudostratified ciliated columnar epi.(trachea)
5)Stratified squamous epi.(esophagus)
6) Transitional epi.(bladder)
3.Lab report (content of lab , drawing)
Tissue Processing
1.Fixation: 10% Formalin
2.Dehydration:graded
concentrated ethyl alcohol
3.Clearing:xylene,infiltrate
with paraffin solvent.
Loss of fat due to ‘clearing’
Tissue Processing
4.Embedding
5.Sectioning
Tissue Processing
6.Staining
Common Stains: Hematoxylin & Eosin
Ross, 5th ed, fig 1.1
Common Stains
Hematoxylin and eosin ("H&E")
Wright’s stain
Relative Sizes in Histology
Standard Units of Measure
Relative Sizes in Histology
Standard Units of Measure
micrometer: 10-6 meter
nanometer:
10-9 meter
Changes in apparent shape,
due to orientation of sections
Ross, 5th ed, fig 1.11
Changes in apparent shape,
due to orientation of sections
Netter/Ciba
Junqueira fig 1-30
Epithelium
Epithelium
•No blood vessles, nourishment is by
diffusion
•Epithelium is innervated
•polarization
Trachea
Classification of Epithelia
•Number of cells
•Shape of surface cells
1. Simple
1. Squamous
2. Pseudostratified
2. Cuboidal
3. Columnar
3. Stratified
4. Transitional
Stomach (NO 25): mesothelium HE ×400
Simple squamous epi.
Small intestine (NO 1): endothelium HE × 400
Thyroid (NO 35): HE × 100
Thyroid: HE × 400
Simple cuboidal epi.
Small intestine (NO 1): villus HE × 400 (Drawing )
Simple columnar epi., Goblet cells, striated border (microvilli)
Trachea
Pseudostratified ciliated columnar epi
Trachea: HE × 400
Pseudostratified ciliated columnar epi.
Esophagus (No.2)
Stratified squamous epi.
Esophagus: HE × 100
Transitional epithelium
distended
contracted
Bladder
Bladder (NO33): HE × 40
Bladder: HE × 100
Transitional epi.
Bladder: HE × 400
Transitional epi.
The end!