Biology 22
Molecular Laboratory Report
1. Bacterial Transformation
2. Plasmid Isolation
3. RFLP Analysis
RESULTS ONLY due on April 21st, 2011
Discussion will be April 22nd in class
Guidelines for the Results
 Different science courses and scientific journals use varied approaches for
the presentation of experimental Results but this molecular laboratory report
will have only the presentation of the data but not a discussion of the data
within the Results section.
 The next few slides provide guidelines for preparing and submitting your
Results.
 The legends are not where data are discussed; simply present what the table
or figure presents.
 Again, the Result section is not the Discussion and must not include a
discussion of the results.
 Onto the specifics of the Results…
Guidelines for the Results
Include a Title Page
 Provide a descriptive title
 Your name
 Must include group numbers (not individual lab partners) for each experiment
Results
 All tables have titles and numbers above table
 All figures have legends and numbers below figure
(Discussion: in lab on April 22nd
What your Result Section must
include:
•
A Table that includes the Transformation results. The Table should be numbered and have a Title. The Title goes
above the Table.
•
The Calculation of your Transformation efficiency
– If you did not obtain any transformation; this should be mentioned in your discussion; however since
everyone observed results from a group who did have results from this experiment it is acceptable to use this
for your transformation efficiency.
•
A Photograph with a legend or caption of the isolated plasmid. The caption and figure number both are placed
below the figure.
•
A Photograph with a legend or caption of the RFLP. The legend or caption should be numbered and go beneath the
figure.
•
A graph of the Standard Curve with a legend or caption.
•
A Table (with title) that summarizes the sizes and distances migrated for the ladder
•
A Table (with title) that summarizes the sizes and distances migrated of the plasmid fragments from each appropriate
lane
•
Individual Maps for each restriction digest. Consider these maps collectively as a single figure so include a caption
with this figure.
•
Maps are not made for the ladder or for the uncut plasmid
Results: Transformation
• Table: results of 3 agar plates: number and color of colonies
• Calculation: of the Transformation efficiency
Results: plasmid isolation
The photograph of the plasmid isolation.
You should include a numbered legend or caption to this
photograph. The legend includes: what samples are present
on the gel, their “lane” number, and the type and percent gel.
Be sure and number the lanes of the gel.
Note: The gels were a 1.5% agarose
Results
 A photograph of the RFLP analysis with figure legend or caption
and number; the legend should be placed beneath the photograph.
 Be sure and number the lanes of the gel.
Results
Standard curve
DNA
bp
Distance migrated (cm)
Results
 A separate table that shows the size (base pairs) of fragments versus
distance migrated of your ladder (known) fragments. You may not be able
to identify all 9 fragments of your ladder but you should be able to identify
approximately 5 to 6 (or more) of these fragments.
 A table that shows the size (base pairs) versus distance migrated of your
unknown fragments
Provide MAP Results
maps for each of the restriction digests.
Examples of MAP Results
Alu1
 These are just
examples!
Total Size:
7000 bp
Lane 3
You will have third
map with both
enzymes and various
sizes shown.
Hae
2550
4500
Total size
7050 bp
Lane 4
Hae
Number of Maps
• Lane 3
• Map 1
• Lane 4
• Map 2
• Lane 5
• Map 3
A Discussion and data interpretation
will be “in-class”.
The End