Van Roekel IB BIO II 4/14/14 Used to calculate frequencies of alleles, genotypes, or phenotypes within a population Useful in determining how fast a population is changing or in predicting the outcomes of matings or crossings. Based off of Punnett squares and probabilities to model allele and genotype frequencies When looked at individually, the frequencies of alleles on chromosomes must add up to 1 p+q=1 p = frequency of dominant allele q = frequency of recessive allele p = 0.5 q = 0.5 T t T TT Tt t Tt tt We are interested in diploid organisms that carry two copies of any trait, so… (p+q)2 = 1 p2 + 2pq + q2 = 1 p2 = frequency of homozygous dominant q2 = frequency of homozygous recessive 2pq = frequency of heterozygote TT = p2 = ¼ tt = q2 = ¼ Tt = 2pq = ½ p2 + 2pq + q2 = 1 ¼ + ½ +1/4 = 1 T t T TT Tt t Tt tt What is the Hardy-Weinberg Equation and what does each variable represent? What does this equation tell us? (p+q)2 = 1, or p2 + 2pq + q2 = 1 p2 = homozygous dominant frequency q2 = homozygous recessive frequency 2pq = heterozygous frequency p = dominant allele frequency q = recessive allele frequency Used to calculate frequencies of alleles, genotypes, or phenotypes within a population Useful in determining how fast a population is changing or in predicting the outcomes of matings or crossings Populations being studies must be a large population, ideally infinite Random mating between individuals with the particular alleles being examined, aka the trait is autosomal There must be a constant allele frequency over time There is no allele specific mortality (sickle cell anemia) There are no mutations that could introduce new alleles There is no emigration or immigration which would alter allele frequency Consider a disease caused by a recessive allele t. The predicted allele frequency in a population in 10%, what is the frequency of the healthy allele in the population? Answer: 90% p+q=1 1-q = p 1-.10 = .90 A study examined 989 members of the previous population, it was found that 11 people had the disease. Calculate the allele frequency of the recessive allele t. Answer: 10.5% 11/989 = .011 = q2 = genotype frequency Square root of .011 = 0.105 = 10.5% = q Calculate the allele frequencies and genotype frequencies of the population in example 1. Calculate the number of carriers in 500 members of the population Allele Frequencies: q = 0.10 or 10% p= 0.90 or 90% Genotype Frequencies: q2 = .01 or 1% 2pq= 0.18 or 18% p2 = 0.81 or 81% Carriers = 90 people (500 x 18%) Using information from example 3, calculate the number of people in 500 members that do not suffer from the disease. Answer: 495 people p2 + 2pq = 81% + 18% = 99% 99% x 500 = 495 Use binomial nomenclature to name and classify organisms 1st word refers to the genus, 2nd word to the species, i.e. Homo Sapiens. Carolus (Carl) Linnaeus consolidated and popularized binomial nomenclature Reasons: ◦ ◦ ◦ ◦ Make sense of biosphere Identify unknown organisms Show evolutionary links Predict characteristics shared by members of a group Five Kingdoms ◦ Kingdom Plantae (plants) ◦ Kingdom Animalia (animals) ◦ Kingdom Fungi (fungi and molds) ◦ Kingdom Protoctista (protozoa and algae) ◦ Kingdom Prokaryote (bacteria) Within each kingdom, there are several subdivisions, called taxa Seven-level hierarchy of taxa: ◦ Kingdom ◦ Phylum ◦ Class ◦ Order ◦ Family ◦ Genus ◦ Species King Phillip Came Over For Good Soup Taxa Human Garden Pea Kingdom Animalia Plantae Phylum Chordata Angiospermae Class Mammalia Dicotyledoneae Order Primate Rosales Family Hominidae Papilionaceae Genus Homo Pisum Species sapiens sativum Feeding Habits (carnivore/herbivore) Habitat (land dwelling/aquatic) Daily activity (nocturnal/diurnal) Risk (harmless/venomous) Anatomy (vertebrates/invertebrates) System of classification must be clear, consistent, easily implemented and a general consensus to apply it. Four of the several types of plant phyla include: ◦ Bryophyta: short in stature such as moss ◦ Filicinophyta: ferns and horsetails ◦ Coniferophyta: coniferous, pine trees cedar, juniper, fir ◦ Angiospermophyta: all plants that make flowers and have seeds surrounded by fruit Vegetative Characteristics such as leave types and stems ◦ Bryophytes: non-vascular, lack vascular transport tissue such as xylem or phloem ◦ Filicinophyta: vascular plants, small leaves ◦ Conifers: vascular, all produce woody stems and leaves are needles or scales ◦ Angiosperms: vascular and have flowers and fruit Reproductive characteristics ◦ Bryophytes: produce spores (microscopic reproductive structures) transported by rain water ◦ Filicinophytes: produce using spores in a similar manner ◦ Conifer: use wind to help reproduce by pollination, produce seed cones with seed scales ◦ Angiosperms: produce seeds, rely on birds, insects, and mammals to transport pollen. Sexual organ is flower, fruit is enlarged ovary Six of many animal phyla include: All listed phyla are invertebrates ◦ Proifera: sponges ◦ Cnidaria: sea jellies (jellyfish), coral polyps, and others ◦ Platyhelminthes: flatworms ◦ Annelida: segmented worms ◦ Mollusca: snails, clams, octopi, etc… ◦ Atrhtropoda: insects, spiders, crustaceans, etc… Porifera: ◦ Simple marina animals that are sessile (stuck) ◦ Feed by pumping water through tissues and filtering out food ◦ No muscle, nerve tissues, or internal organs Cnidaria: ◦ Very Diverse: Coaral, sea anamones, jellyfish, hydra, Portuguese man-of-war ◦ All have stinging cells called nematocysts ◦ Some sessile, some free swimming, some both ◦ Gastric pouch for digestion Platyhelminthes: ◦ Flatworms with one body cavity, gut with one opening for food to enter and waste to leave ◦ No heart, no lungs ◦ Exchange gas by diffusion ◦ Example: Tapeworms Annelida: ◦ Segmented worms such as earthworms, leeches, and polychaetes ◦ Bodies divided into sections separated by rings ◦ Have gastric tracts, w/ mouth at one end and anus at opposite Mollusca: ◦ Aquatic animals, snails, clams, octopi ◦ Shell produced with calcium ◦ Non-segmented bodies Arthropoda: ◦ Hard exoskeleton made with chitin, segmented bodies, and limbs (walking, swimming, eating) ◦ Insects, spiders, scorpions, crustaceans such as crab and shrimp ◦ Live in most habitats throughout world ◦ Vary in size Used to help identify which order, genus, and species an organism is by using observable characteristics In General: ◦ Look at first section of key which has a pair of sentences ◦ Look at the organism to see if particular characteristics are present ◦ If answer is yes, to go end of line/next section that contain a new pair of statements to examine ◦ If answer is no, go to second statement just below it and follow that one, should it be true ◦ Continue this until the end of the line has a name, not a number and if each question was answered correctly, should be your organism. Example in book, pg. 149 1. a. Organism is living........................................................go to 4. 1. b. Organism is nonliving..................................................go to 2. 2. a. Object is metallic........................................................go to 3. 2. b. Object is nonmetallic..................................................ROCK. 3. a. Object has wheels......................................................BICYCLE. 3. b. Object does not have wheels......................................TIN CAN. 4. a. Organism is microscopic...................................PARAMECIUM. 4. b. Organism is macroscopic............................................go to 5. 5. a. Organism is a plant.....................................................go to 6. 5. b. Organism is an animal.................................................go to 8. 6. a. Plant has a woody stem..............................................go to 7. 6. b. Plant has a herbaceous stem.................................DANDELION. 7. a. Tree has needle like leaves.....................................PINE TREE. 7. b. Tree has broad leaves............................................OAK TREE. 8. a. Organism lives on land................................................go to 9. 8. b. Organism lives in water...............................................CLAM. 9. a. Organism has 4 legs or fewer......................................go to 10. 9. b. Organism has more than 4 legs...................................ANT. 10 a. Organism has fur........................................................go to 11. 10 b. Organism has feathers................................................ROBIN. 11 a. Organism has hooves.................................................DEER. 11 b. Organism has no hooves............................................MOUSE. Vocabulary can be challenging and technical Make sure using the right key, no key can identify all the species Making a Dichotomous Key ◦ Start by putting things in groups by identical characteristics ◦ Invent statements that divide things into created groups