UNIT 3 The transmission of traits WHAT IS A TEST CROSS? Biology and Geology 4. Secondary Education. UNIT 3 What is a test cross? The additional experiment which Mendel carried out, to prove that alleles come in pairs and separate at the moment when gametes form, consisted of crossing F1 (yellow seeds) with the pure paternal line of green seeds (recessive homozygote). Let’s take the yellow seed to be Aa. In this case, 50% green seeds and 50% yellow seeds are obtained. If the yellow seed had been AA, all of the seeds would have been yellow (Aa). Biology and Geology 4. Secondary Education. Yellowamarilla seed F1F1 Semilla Aa Green Semillaseed verde x a aa a A Aa Aa aa aa a