The Dollar Store, Duct Tape and Creativity: Maintaining an Undergraduate Research Program on a Shoestring Budget Anna Yeung-Cheung, Ph.D., Nancy Todd, Ph.D., and Wendy McFarlane, Ph.D. Department of Biology, Biochemistry and Environmental Studies, Manhattanville College, Purchase, New York Abstract Abstract Title Manhattanville College is a small liberal arts college founded in 1917. There is a strong emphasis on teaching at the college, but faculty are also required to develop their scholarship to achieve reappointment, promotion and tenure. The Biology major incorporates a research sequence as its capstone project. Each year, 10-25 students conduct research with their mentors to produce potentially publishable papers, and many students travel to conferences to present their research. Funding for research has traditionally been limited, although more funds have been allocated in recent years. As a result, the faculty have had to be creative about developing, implementing and conducting research projects. With such limited support, we have been surprisingly successful at producing quality research and well-trained graduates who have gone on to successful careers of their own! Examples of Research Activities and Projects We try to reuse and recycle common everyday products Food preference in the Asian Shore Crab Angelica Scaglione ‘10 Kept the crabs from cannibalizing each other by using baby food containers collected by her mentor Total Cost: $0.00 Nutrient Substrates and Development of Dermestes maculatus Kirsten Betsill ’11 Nicole ‘11 Yasmin Reyes ’12 Glad plastic containers, bridal tulle and duct tape Total Cost: $200.00 Funded by TriBeta Grant Manuscript in prep. Background Sometimes we build or create components of our research projects Manhattanville College has an amazingly diverse mix of 1700 undergraduate students from more than 30 states and 50 countries, as well as ~1,000 graduate students. The Department of Biology has 5 full time professors, several adjunct professors, and 40-50 major students each year. Our capstone experience includes a 3-semester research project in which students work closely with a research mentor. This sequence includes: Bio 3099 Research Seminar (Junior Fall semester, 2 credits) Bio 3499 Senior Research (Senior Fall semester, 2 credits) Bio 3998 Senior Evaluation (Senior Spring semester, 2 credits) At the end of this sequence, students write a potentially publishable research paper and give a final presentation to their mentors and peers. Our current research budget for up to 25 seniors a year is only $7000.00, and we have limited amount of funding for students to present their work at conferences. Prior to 2008, we had no research line in our budget.. We have had some success with obtaining small research grants ($250-$3000), but the overall cost of research in terms of materials and space is not completely covered. As a result, we have had to be creative with our projects and seek out alternative sources of materials for these projects. Hand-made electromagnetic coil to examine the effects of 60hz EM fields on plant growth Total Cost: Growlights, wire, seeds Total Cost: $175.00 We have developed many relationships with nearby parks and associations We encourage “Field” studies to use the natural resources around our location: Hudson River, Mamaroneck Harbor, Long Island Sound, Appalachian Trail, Black Forest, Marshlands Conservancy, Sheldrake Environmental Center to name a few. We have an Environmental Park with Ecoclassroom and Ecomachine for purifying water on campus It provides an excellent area of research within Environmental Studies, reducing the need to travel or pay for research permits for study purposes. The impact of water quality on early embryonic development of the green sea urchin (Strongylocentortus drobachiensis) Stephen Corvini and Janyll Perez ’12 Stephen is studying Physical Therapy at NY Medical College Total Cost: $50.00 Maryke Van Leewen ‘01 B.S. and M.S. in Nursing, Johns Hopkins University Total Cost: $80.00 ENVIRONMENTAL/MICROBIOLOGY LAB AFTER Many students have the opportunity to present their research at scientific conferences, and some projects are ultimately published. Senior Research Projects 2008-2012 99 Papers and Presentations Presentations at National Science Conferences 2008-2012 19 presentations Presentations at Undergraduate Conferences 2008-2012 51 presentations Scientific Publications by Undergraduates 2007-2012 9 publications 3 manuscripts currently in review 2 currently in preparation On many occasions, the student’s research project has been the deciding factor in their admission to graduate and medical schools. Our graduates have been very successful in their careers, and we have been able to keep track of 111 graduates since 1999. Total Cost: $200.00 A Mini-Gunderboom filter to test efficacy of Gunderboom technology for filtering water in the lab, built in Hong Kong 1 gallon milk jugs, panty hose, paperclips and an airstone ENVIRONMENTAL/MICROBIOLOGY LAB BEFORE Graduate Activities 1999-2012 Jake Fried ‘08 Completed NYU College of Dentistry Used an old refrigerator to keep urchin larvae alive, developed new method for oxygenation in the unit GENERAL BIOLOGY LAB AFTER GENERAL BIOLOGY LAB BEFFORE Undergraduate Scholarship Alyssa is teaching 6 Grade Life Science in the Bronx, NY New egg collecting apparatus Total Cost: $50.00 Recent renovations and lab upgrades have resulted in a well-equipped tissue culture laboratory to conduct in vitro lab projects Cell lines are frozen to save for future use rather than additional purchases, and some cell lines are donated from local research laboratories. Renovations of the teaching laboratories have also provided additional space for research Yeung-Cheung, A. K., N. M. Benevento and D. M. Pavel. 2009. An evaluation of the effectiveness of the Gunderboom® in protecting waters from bacteria. Journal of Environmental Health 71(8) 42-27 A new breeding and egg collection protocol for Danio rerio, with notes on variability in hatching time Nancy Todd, Hiroshi Osaka and Alyssa Fico ‘12 Paper under review in Zebrafish th Sometimes, we reuse/recycle items from home or other projects! Total Cost: $30.00 Teamwork There are often pairs or teams of students working on different aspects of the same project. The effect of the Gunderboom® water filter in bacteria levels in Mamaroneck Harbor – Total Cost: $2000.00, funded by a grant from Gunderboom® Nicole Benevento, Pretima Persad and Donatella Pavel ’10 Crystal Noujaim ’07 Ph.D. in Physical Therapy from New York Medical College, 2010 How do we do it? We find and purchase nonchemical supplies through local retail stores or on EBAY. Some research projects are collaborations between 2 professors and another institution Mame Cisse ’12 NY Medical College and Manhattanville Total cost to us: $0.00 Recent Improvements Exercise stress and muscle physiology Students exercised mummichog fish to exhaustion in a bucket, then froze tissues for biochemical analysis Tara Pisano & Alex Braverman ’11 Tara is at Wagner College of Nursing Thomen, A. ‘08, W.J. McFarlane, and N.E. Todd. Testing the efficacy of a small-scale Ecomachine to improve water quality and allow for wetland restoration. Manuscript in prep Total Cost: $300.00 Acknowledgements We recently started a composting project with reused wood pallets and food scraps from the campus cafeteria which was used to start an organic garden on campus. Organized by Dr. Christopher Pappas and students Environmental Jobs in the field Health Science Jobs in the field Research Labs Teachers Actress Medical School Dental School Osteopathic School Optometry School 5 18 10 11 1 7 5 3 2 Physicians Assistant Programs Nursing Programs Speech Language Pathology Programs Chiropractic Homeopathic Medicine Ph.D. Programs Ph.D. Programs in Molecular Biology Ph.D. Physical Therapy M.S. Programs Bioinformatics Education Cell and Molecular Biology Biomedical Sciences Nutrition Special Education Public Health Epidemiology Environmental Studies 2 7 1 1 1 4 2 4 1 6 2 3 2 2 5 1 1