Chapter One Themes in the Study of Life Characteristics of Life Life can’t be summed up into a simple, one-sentence explanation How do you know that a tree is alive and a rock is not? All living organisms share certain characteristics Do you remember what some of these are? Reproduce Contain DNA Adapt to environment Grow and develop Made up of cells Have definite lifespan Utilizes energy Responds to stimuli Levels of life Life is organized into many structural levels. Biological structural levels build upon the level below it cells tissues organs systems Individual organism populations communities ecosystems Biosphere *And cells of course are made up from even smaller parts. Each Level of Biological Organization Has Emergent Properties Continued Order is all around us. Notice the pattern and order in this sunflower. Emergent properties within these levels manifest themselves as a result of dynamic and unique interactions between components Understanding Biological Organization and its Many Levels is Fundamental to the Study of Life Scientists cannot fully explain a higher level of order from a critical analysis of it partsFor example: what good is the handle of a hammer without the head? Holism = disrupting a living system interferes with the meaningful explanation of its processes But, biological structural levels build upon the level below it, so scientists cannot critically analyze living systems without taking them apart Reductionism = reducing complex systems to simpler components that are more manageable to study Cells are an Organism’s Basic Units of Structure and Function The Cell Theory = all living things consist of cells & cells come from other cells Prokaryotic cells = bacteria / NO true nucleus Eukaryotic cells = all other organisms / membranebound nucleus and endomembrane system In order for a species to continue, organisms must reproduce. DNA makes it possible for the heritable information to be passed on. DNA - deoxyribonucleic acid Encoded biological instructions Substance of genes Units of inheritance DNA structure Long chain made of four nucleotides Adenine, cytosine, guanine, and thymine Specific sequential arrangements encode the precise information in a gene Structure and Function are Found at all Levels DNA encodes a message which dictates the growth and development of the organism Each cell, organelle, organ, etc. has its own function or duty to perform. In multicellular organisms, genes are turned on or off in order to specialize the functions of the cells Form fits function Analyzing a biological structure clues us to what it does and how it works Knowing function of a structure provides insight about its construction Understand your subject or specimen Cells may be the units of organisms but it is the organism that is the unit of life It is not enough to merely learn what parts make up an organism, but how it grows, interacts, lives, and responds. Questions to ask about any organism to be studied: What kind of organism is it? Where does it live? How does it acquire nutrients and other resources from the environment? How is the organism equipped for its way of life? Diversity and Unity Nearly 2 million species of living things have been identified, but it is estimated there are between 5 - 30 million total species on Earth ~260,000 plants ~50,000 animals ~750,000 insects Everything has its own niche and purpose in life (diversity and uniqueness), but every living creature needs interaction and support to serve a greater purpose (unity) Taxonomy With so many types of organisms, a system of classification was necessary Carolus Linnaeus Bionomial nomenclature Taxonomy - naming and classifying species Kingdom Phylum Class Order Family Genus Species KINGDOM Animalia PHYLUM Chordata CLASS Mammalia Figure 22-1(1) Page 422 ORDER Carnivora FAMILY Felidae GENUS Felis SPECIES Felis catus Figure 22-1(2) Page 422 6 Kingdom vs. Domains The six kingdom system of taxonomy categorizes organisms based on cell structure and nutrition The domain system on the other hand is based on molecular differences There are 3 domains: Domain Archaebacteria - contains the archaea kingdom Domain Eubacteria - contains the eubacteria kingdom Domain Eukarya - contains kingdoms animalia, protista, fungi, and plantae *Phylogeny will be discussed when we get to evolution unit Domain Eubacteria (bacteria) Figure 22-2 Page 423 Domain Archaea The three domains Common ancestor of all living organisms Domain Eukarya Kingdoms There are 6 Kingdoms: Archaea - extreme bacteria; more related to euks Eubacteria (Monera) - typical bacteria; prokaryotic; peptidoglycan cell walls Protista - single-celled, aquatic, euk Fungi - multi-celled; euk, decomposers Plantae - photosynthetic; euk; multi-celled Animalia - consumers; euk; multi-celled; 9 phyla Eubacteria Archaebacteria Protista Plantae Protista Figure 22-3 Page 424 Animalia Common ancestor of all eukaryotes The six-kingdom system of classification Common ancestor of all living organisms Fungi Bacteria We’ll study in depth during microbiology and DNA technology Protista Protists produce 70% of our oxygen, but not all of them are photosynthetic Vary in size, structure, mode of locomotion, and reproduction Endosymbiont Theory: the accepted belief is that mitochondria and chloroplasts originated from symbiotic relationships between early protists and bacteria Chloroplasts - from photosynthetic prokaryotes Mitochondria - from aerobic prokaryotes Divided into 8 major groups: Excavates, opisthokonts, discicristates, alveolates, heterokonts, cercozoa, amoebozoa, and plants NO- you don’t need to memorize these The plant category also includes plants and algae Opisthokonts include members of the animal kingdom and fungi kingdom as well too confusing of a system Fungi Most are filamentous; made up of hyphae forming an aggregation called mycelium Most have cell walls of chitin Most produce by spores (produced sexually or asex) 5 phyla are identified (but only 4 true phyla exist) Chytridiomycetes (most primitive) -only fungi with flagella Zygomycetes - black bread molds Ascomycetes - sac fungi (yeasts, mildews, colored molds, morels and truffles) Basidiomycetes - mushrooms, rust, smut, and puff balls Some fungi play an important ecological role Mycorrhizae - a type of fungus which decomposes organic material in soil Benefit plants by increasing their absorptive surface area Roots supply fungus with sugars, amino acids, and other organic substances Scientists have measured movement of organic materials from one tree species to another without with Plants You’ll learn everything you ever wanted to know about plants before we do photosynthesis in a few weeks. Animalia Animals can be classified according to: body symmetry - radial or bilateral symmetry or asymmetrical body cavities: coelomates, acoelomates, pseudocoelomates Coelom : a fluid-filled cavity lined with mesoderm evolutionary relationships based on molecular data (DNA, rRNA, and Hox genes) Animal Phyla 1. Porifera - sponges Aquatic, mostly marine, typically asymmetrical, multicellular but do not have tissues, regenerative capabilities 2. Cnidarians - hydras, anemones, coral, jellyfish Radially symmetrical, stinging cells called nematocysts Diploblastic (only 2 germ layers) Animal Phyla Acoelomates: 3. Platyhelminthes - flatworms (flukes, tapeworms) No body cavity, bilaterally symmetrical, simple nervous system with ganglia Triploblastic (endoderm, mesoderm, ectoderm) Animal Phyla Pseudocoelomates: 4. Nematodes - round worms (hookworms, pinworms, Trichina) All parasitic, lack a circulatory system, covered by cuticle, false body cavity between mesoderm and endoderm Triploblastic (endoderm, mesoderm, ectoderm) Animal Phyla Coelomates: I - Protostomes 5. Molluska - squid, snails, clams, oysters Soft body (usually covered with a shell), foot for locomotion, visceral mass contains body organs, reduced coelom, mantle secretes shell (if present), open circulatory system (except in cephalopods octopus, squid, nautilus), triploblastic 6. Annelida - segmented worms (earthworm & leech) Segmentation, setae, closed circulatory system, triploblastic Animal Phyla Coelomates: I - Protostomes 7. Arthropoda - crustaceans, trilobites, centipedes, arachnids, insects Exoskeletons, paired, jointed appendages, open circulatory system, nervous system of brain, ladder-like nerve cords and often compound eyes Aquatic arthropods have gills while terrestrial arthropods have book lungs or trachea Triploblastic (endoderm, mesoderm, ectoderm) Animal Phyla Coelomates: II - Deuterostomes 8. Echinodermata - sea stars, sea lilies, crinoids, sand dollars Larval stage is bilaterally symmetrical and adult form is radially symmetrical Nerve net, no excretory organs, endodermis, calcareous plates covered by epidermis, tube feet Triploblastic (endoderm, mesoderm, ectoderm) Animal Phyla Coelomates: II - Deuterostomes 9. Chordata - vertebrates and invertebrates At some point in life cycle, all chordates have: Notochord (flexible supporting rod), dorsal hollow nerve cord, pharyngeal slits, and post anal tail Coelomates with bilateral symmetry, endoskeleton, closed circulatory system with ventral heart Triploblastic (endoderm, mesoderm, ectoderm) Animal Phyla A closer look at vertebrates: 2 pairs of appendages, closed circulatory system with ventral heart, vertebral column of bone or cartilage, anterior cranium, paired kidneys, complete digestive tract with large digestive glands Divided into 3 classes of fish (osteichthyes, chondrichthyes, agnatha) and 4 classes of tetrapods (amphibians, reptiles, birds, mammals) Germ Layers We’ve used the term triploblastic, but what does it mean? 3 germ or embryonic layers: Ectoderm - becomes outer covering and nervous sytem Mesoderm - becomes most internal organs Endoderm - becomes lining of the gastrointestinal tract Quantifying Biology Biology is the study of any and/or all of these living organisms we just mentioned Biology does not only involve observations, but includes quantifying, manipulating, and analyzing data in order to draw conclusions Review the Scientific Method: Observations, Problem/Question, Hypothesis, Experiment and Data Collection, Analysis and Conclusions, Repetition and Sharing Let’s focus briefly on manipulating and interpreting data Data Collection All data collected should be accurately measured or counted Should also be recorded carefully and neatly Data tables should be done with ruled lines and with appropriate titles and units Statistical Analysis Correctly manipulating data enables scientists to demonstrate significance of data and to validate claims When you learn of a claim that a scientist is making, don’t you want to know what the supporting data is? Statistics Terminology Mean = arithmetic average (sensitive to outlying data) Median = the value that splits the number of data entries in half (not sensitive to outlying data) Range = the spread of the data from least to greatest Standard deviation = a calculated value indicating how much the data varies around the mean value Error bar = vertical reference lines on graphical distribution of data that illustrates points of std deviations To calculate Standard Deviation manually: Take EACH data point and subtract the mean value Square each of these values and add them up Divide this sum by (the number of data entries minus 1) Then, take the square root of the answer The result is the Standard Deviation and is denoted with a +/- sign because it falls on either side of the mean value Standard Deviation using the TI Calculators: Hit <STAT> and <1> to get to the lists screen In the L1 column, enter your data Hit <STAT> again Choose CALC and then 1-Var Stats Hit <ENTER> It gives you the mean, the sum of the values, the sum of the squared differences, and the standard deviation (Sx) (If you scroll down, it also gives you the min and maximum values and the median) t-test = a test used to deduce the significance of the difference between two sets of data On your TI Calculator: Hit <STAT> and <EDIT> to enter your lists in column L1 and column L2 Hit <STAT> again and scroll over to <TESTS> Choose <4> for two sample T test Make sure the two column heading appear from your data Scroll down and choose calculate The t value and df (degrees of freedom) are given Now, use your table to determine acceptance Correlation When a correlation exists between two sets of data, this does not establish that there is a causal relationship between the two variables being examined