The study of cells All living things are composed of cells Cells are basic units of life New cells come from pre-existing cells BUT…. How did the very first cell get here???? Viruses lack typical cell structure, use host cells to reproduce Mitochondria and chloroplasts have their own genetic material/can reproduce independent of cell Small Simple No Nucleus No membrane bound organelles Then what do they have? Cell membrane Cytoplasm DNA: Circular, not in nucleus Ribosomes Bacteria Prokaryotic Structure cytoplasm with ribosomes DNA cell membrane More complex Larger Plant cells DNA in nucleus in long strands Contain membrane bound organelles Specialized compartment with a specific function Animal cells Fungi (Yeast) Oh where, oh where do we find cell organelles? Cytoplasm (protoplasm): The fluid inside a cell, but outside the nucleus “Holds” organelles in place Site of cellular chemical reactions Cyclosis: Streaming movement of cytoplasm Cytoplasm Cytoplasm Nucleus: Controls cell processes Contains hereditary information (DNA) Nucleolus: Ribosome formation Nucleus Nucleolus Nucleolus Nucleus Mitochondria Release energy from food to make useable energy (ATP) Outer and inner membranes Inner membrane folded (Increased surface area) Found in: Animal cells Plant cells Contain their own DNA! Mitochondria Mitochondria Chloroplasts Site of photosynthesis Convert light energy to chemical bond energy Only in plant cells Contain their own DNA! Chloroplasts Animal cells don’t have chloroplasts!!! Ribosomes Site of protein synthesis Can be free in cytoplasm or attached to endoplasmic reticulum Ribosomes Ribosomes Endoplasmic reticulum “ER” A system of membranous sacs Assembles components of cell membrane Protein modification Rough endoplasmic reticulum Protein synthesis Rough: Ribosomes RER Smooth endoplasmic reticulum Contains enzymes needed for cell Ex. enzymes for lipid synthesis Smooth: No ribosomes SER Rough ER Smooth ER Smooth ER Rough ER Golgi Who? apparatus, complex, bodies Marks proteins for use in cell or export (adds address w/proteins) Produces digestive enzymes Golgi body Golgi body assorted vesicles Golgi body smooth ER rough ER Chains are assembled on ribosomes in cytoplasm. DNA instructions for building protein chains leave the nucleus and enter the cytoplasm. Lysosome Packaged by Golgi body Membranous sac filled with digestive enzymes “Suicide Sacs” Lysosomes Lysosome Lysosome Vacuole Cell storage: Water Food Wastes Plants: One large central vacuole Animals: Few small vacuoles Vacuoles are crucial in single-celled organisms to maintain homeostasis Vacuole Cell Wall Provides support Provides shape Made up mostly of cellulose Animal cells don’t have! Cell wall Animal cells don’t have cell walls!!! Centriole Used in cell division Animal cells only Plant cells don’t have centrioles!!! Centriole Cilia: Locomotion Moving substances across surface of cell Flagella: Locomotion Cilia Plasma membrane/cell membrane Plant AND animal cells Controls what goes in and out of cell Protection Support Cell membrane Cell membrane Lipid Bilayer • Main component of cell membranes lipid bilayer fluid fluid Cell (Plasma) Membrane Structure ☼ 2 layers of phospholipids (lipids with phosphate groups attached) ☼ Proteins - embedded in lipid layers- for transport or receptors ☼ Carbohydrates - attached to surface proteins or lipids – markers ☼ Lipids & Proteins can move along membrane = “Fluid” FLUID MOSAIC MODEL of Cell Membrane Receptors recognize substances (hormones etc.) and allow them to enter cell Animal Cells Plant Cells Cell Walls NO YES Chloroplasts NO YES Centrioles YES NO 1 or 2 100 + Golgi Bodies Vacuoles Many Small Few Large Cell wall Chloroplasts Cell membrane Vacuole Mitochondria Ribosomes Lysosome Golgi body Nucleus Nucleolus Smooth endoplasmic reticulum Rough endoplasmic reticulum Cytoplasm Golgi body Cilia Cell membrane Centrioles Smooth endoplasmic reticulum Lysosomes Free ribosomes Mitochondria Nucleolus Rough endoplasmic reticulum Nucleus Cytoplasm Staining Techniques Applying stain makes cell structures more visible Can be useful in identifying organisms Example: Methylene blue Organelle Cell Tissue Organ Organ System Organism